Gene Details:

Functional Descriptions:

  • The phenotypes of fern-like were fully reversed in complemented lines, indicating SlFERN is the correct candidate.
  • Plant expression vectors containing the full-length coding sequence (CDS) of SlFERN (Solyc06g075000) or mtHD (AT5G60335) fused with the GFP gene were transiently expressed in epidermal cells of N. benthamiana leaves.
  • Our work revealed that a single base mutation occurs in SlFERN, leading to a Cys-to-Tyr conversion and a reduced level of FERN protein.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Solyc06g075000.3.1
    GATCCGTATACAGCTGAAAAGTGTGGCATAGATCTTTATTTTTTTTTTAAAACACACATAACCCGCCGCTAGTAAACCTTAGTGTATAAAAGCCTGTAAACCACTCTAATGTAAGCTACATTAAGCAAATTTTGTGCGATAGATTCGATTCAGAAGACACTAAGGAGAAATTGATCTCGAGTCATTTATATAATAATTATCCTTTATACCGTCATCCGAAGAATATCCGAAGAATAAATTTGTTTTTTTTGACAACTAAAATAATAATTTTCCATACATTTAAAGTTGGAGTGATTTTTATGTAGTTATAAAAGACTAAAATGATCGATTTTATGACCTATCTATTGGTACAAAGAACTAAGGATATTCCAAGCCTCAGACTGCACACAAACTGTTTGATGATTTGTTTCAAAGAGCCCTCACATTCAGATACTTAATTGTTCTTTATCTGTTCGTGATCAAAAAAATCTTGAAGTGCGATCAGTTAACTGTATCATTGGATCTTTACCTACAGGTGGAAGCTGGTACCCTACCTGCTTTACCACATTGAAGACGAATTGTCCATTGTCTTTGAAGTTTGTGGAAGATGCTTATGAAACGTCTCTTTTCATCTTCACATGTCTTTTCCTCGTCCTCCGCTTCTTCGAATTTGCTTAAAATCGGAAGCGTTTTGAAGCAAGCTAGGACATTTGCAGATGATGATGTTCTTGGTTATTCTAAGCTGACCCATGACGCCAATCCATTGCACTTTGATGCTGAATGCGCCAAGAATGCTGGTTTCACTGACTGCCTTGTTCCTGGGATGCTTGTTGCTTCCTTATTTCCCAGGATTATTGCTGCCCATTTTCCAGGAGCTATCTATGTTTCACAAACTTTGCACTTCAAATTACCTGTTTATATAGGAGACGAGATCATTGCTGAGGTACAGGCAGTAAGCATCAGACAAATCAAAAACAAGTACATCGCAAAGCTGTCAACAAAATGTATCAAACGTGATGGTCCTCTGGTTATAGATGGTGAAGCAACAGCAATGTTACCATCTCTGGTCATGGAACCACCAAACTTGGAAATTACTTCCAGCTAATCGGTAACACCTTTGCTTGTCAGTTAAAACATTCTAGAGCTGCACGAGCTTCTCTTAAGAGAAATACGAAGGGAATTTCCCTGCGTATTGCCTGGATCATTTGCATCAACAAGTTCTACAGCATAGATGTCATTCAGTTCAAAGTCTTTTAAGTTTTCCTAGTGATTAGCAAATGCTCATACTTTGTTCTTACTGATCGATGTTAGATTTGACTCTGGTACTTGCTGCCAAACAAATGTCCTTGCACTAAAACTGTCATTTGAGCATAAAATCTTTATGGAGTCTTGAATTTCGATTCCTTTTGACTCCACCCAATAGAGATATATTCTGCGTAGTCGTTACTTATCATATTAATTAACATGTATTTTTCACATGAATTTATCATTTTATTATGTCCAATTAACAGCAAAAAATGACTAAGTTTGCAATACATGTCTTGCACACTTCTAACAGTTCACTTGCACAGGTGATCAACTTGAAATTTTGAAGCTCCTGTCTAACTAACTTTTTCTTCCAAACTCTAAATTCTCTTTATCTTTTGTACTGTCAATGATCAAACAAGTAAACCACAGAAGAAACAGTTCATAAACTTCACGGAAAGGCATGTGAATAACTTAAGATTTTATTAATCTTAGAGGGAGTAAGTTCAAAGCTAATCCCACTAGCGATGTACTTTTTATCTGAGCTCACATTACAGCTAGAGAACCAGTTGAAAATAACATGGCCTTCTCTTCTAGTCCTTGAAGC
CDS Sequence
  • >Solyc06g075000.3.1
    ATGCTTATGAAACGTCTCTTTTCATCTTCACATGTCTTTTCCTCGTCCTCCGCTTCTTCGAATTTGCTTAAAATCGGAAGCGTTTTGAAGCAAGCTAGGACATTTGCAGATGATGATGTTCTTGGTTATTCTAAGCTGACCCATGACGCCAATCCATTGCACTTTGATGCTGAATGCGCCAAGAATGCTGGTTTCACTGACTGCCTTGTTCCTGGGATGCTTGTTGCTTCCTTATTTCCCAGGATTATTGCTGCCCATTTTCCAGGAGCTATCTATGTTTCACAAACTTTGCACTTCAAATTACCTGTTTATATAGGAGACGAGATCATTGCTGAGGTACAGGCAGTAAGCATCAGACAAATCAAAAACAAGTACATCGCAAAGCTGTCAACAAAATGTATCAAACGTGATGGTCCTCTGGTTATAGATGGTGAAGCAACAGCAATGTTACCATCTCTGGTCATGGAACCACCAAACTTGGAAATTACTTCCAGCTAA
Protein Sequence
  • >Solyc06g075000.3.1
    MLMKRLFSSSHVFSSSSASSNLLKIGSVLKQARTFADDDVLGYSKLTHDANPLHFDAECAKNAGFTDCLVPGMLVASLFPRIIAAHFPGAIYVSQTLHFKLPVYIGDEIIAEVQAVSIRQIKNKYIAKLSTKCIKRDGPLVIDGEATAMLPSLVMEPPNLEITSS*