Gene Details:
- Gene ID: Solyc03g025560
- Gene Symbol: SlCPT3
- Gene Name: cis-prenyltransferase 3
- Genome: Tomato Genome version SL4.0
- Species: Solanum lycopersicum
Functional Descriptions:
- RNAi-mediated suppression of one member of the tomato CPT family (SlCPT3) resulted in a ~60% decrease in dolichol content.
- Co-expression of SlCPT3 and SlCPTBP in yeast and in E. coli confirmed that dolichol synthase activity strictly requires both proteins.
- Organelle isolation and in vivo localization of fluorescent protein fusions showed that both SlCPT3 and SlCPTBP localize to the ER, the site of dolichol accumulation and synthesis in eukaryotes.
- SlCPT3, interacts with the tomato LEW1 ortholog. Both of these proteins were required to fully compliment the yeast rer2∆ mutant, restore microsomal CPT activity, and dolichol synthesis.
Function-related keywords:
Literature:
- A two-component enzyme complex is required for dolichol biosynthesis in tomato. DOI: 10.1111/tpj.12859 ; PMID: 25899081
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Solyc03g025560.4.1
TTAGTTCTCTTGTTTGTATTTGTATTTGTATTTTGGTCAAAAGCAGAGATCTATCTCTAAACTACATGTGTTAGAGGAGAGACGGGAGAAGAGTCTGCCGCCGACGTTGATTCCTCTAACATCATCAGCCGGCACTGTTTTCTGATCACAACTCAAAGGGTTGATTTTGGAGCATGATCAAGTATTAATTCGAGGGTTGATATGGAAGGTGTGAACGGTAAAAAAGTGGGGCATTTATGTGAAAATATTAGCAGCTTTGTTCGCCAATGTATATTTTCTATACTTAGTGTGGGTCCTGTTCCTAGTCACATTGCTTTTATCATGGATGGAAACCGTAGATATTCTAAGAAACAGAACTTGCTTGATGGGAATGGACATAGAGCGGGATTTTCAGCTCTTATCAATATGCTAAAATATTGTTACGAGCTTGGTGTAAAATATATAACAGTTTATGCATTCAGCATTGATAACTTCAAGCGAAGGCCTGAAGAAGTTGTATCACTAATGAAACTGATGCAGGAAAAAATTGATGAATTAACAAAAGAGGAGAGCATTGTAAACCGCCTTGGTATTAGAATTTACTTTCAAGGAAACCTTAAACTTTTGAGTGACCATGTCAGGTTAGCCGCCGAGAGGGCTATGGTAAAGACATCAGGTAATTCAAAAGCTATATTGTCTATTTGTGTTCTTACCTTATAGCAGAAGAGAACAGGGGTTAGGCCTTCAGCATTGTATCATTTAGCAAAGCATTGAACATCAGCTGAGATGAACTTTTGAAGCCATATGTAGATTTCATCTCCTCTAACTACTTCGATGCTCTGTTTTGTAGTACCACTGAGAATGATTGAAGGTGAAGGGGACATGAAAACCAATTGTGATTCTTGATTCTATCTCAACCAAGTTGTTCAGATAACACTACGAGTTTTTTGTTCAATTGCATCAGTATCTTTTTA
CDS Sequence
- >Solyc03g025560.4.1
ATGGAAGGTGTGAACGGTAAAAAAGTGGGGCATTTATGTGAAAATATTAGCAGCTTTGTTCGCCAATGTATATTTTCTATACTTAGTGTGGGTCCTGTTCCTAGTCACATTGCTTTTATCATGGATGGAAACCGTAGATATTCTAAGAAACAGAACTTGCTTGATGGGAATGGACATAGAGCGGGATTTTCAGCTCTTATCAATATGCTAAAATATTGTTACGAGCTTGGTGTAAAATATATAACAGTTTATGCATTCAGCATTGATAACTTCAAGCGAAGGCCTGAAGAAGTTGTATCACTAATGAAACTGATGCAGGAAAAAATTGATGAATTAACAAAAGAGGAGAGCATTGTAAACCGCCTTGGTATTAGAATTTACTTTCAAGGAAACCTTAAACTTTTGAGTGACCATGTCAGGTTAGCCGCCGAGAGGGCTATGGTAAAGACATCAGGTAATTCAAAAGCTATATTGTCTATTTGTGTTCTTACCTTATAG
Protein Sequence
- >Solyc03g025560.4.1
MEGVNGKKVGHLCENISSFVRQCIFSILSVGPVPSHIAFIMDGNRRYSKKQNLLDGNGHRAGFSALINMLKYCYELGVKYITVYAFSIDNFKRRPEEVVSLMKLMQEKIDELTKEESIVNRLGIRIYFQGNLKLLSDHVRLAAERAMVKTSGNSKAILSICVLTL*