Gene Details:

Functional Descriptions:

  • Abscisic acid levels in tomato ovaries are regulated by LeNCED1 and SlCYP707A1.
  • An important regulator of ABA biosynthesis in tomato is 9-cis-epoxy-carotenoid dioxygenase (LeNCED1), whose mRNA level in ovaries is reduced after pollination. The increased catabolism is likely caused by strong induction of one of four newly identified putative (+)ABA 8′-hydroxylase genes.
  • Because fruit set is induced by GA and auxin, we were interested in the effect of these hormones on the activity of both, the LeNCED1 gene and the SlCYP707A1 gene.
  • Gibberellin and auxin application have different effects on the LeNCED1 and SlCYP707A1 gene expression. The crosstalk between auxins, gibberellins and ABA during fruit set is discussed.
  • Over-expression of LeNCED1 in tomato (Solanum lycopersicum L.) with the rbcS3C promoter allows recovery of lines that accumulate very high levels of abscisic acid and exhibit severe phenotypes.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc07g056570.1.1
    ATGGCAACTACTACTTCACATGCCACAAATACATGGATTAAGACTAAGTTGTCAATGCCATCATCAAAGGAGTTTGGTTTTGCATCAAACTCTATTTCTCTACTCAAAAATCAACATAATAGGCAAAGTCTCAACATTAATTCCTCTCTTCAAGCTCCACCTATACTTCATTTTCCTAAACAATCTTCAAATTATCAAACACCAAAGAATAATACAATTTCACACCCAAAACAAGAAAACAACAACTCCTCTTCTTCTTCAACTTCCAAGTGGAATTTAGTGCAGAAAGCAGCAGCAATGGCTTTAGATGCTGTAGAAAGTGCTTTAACTAAACATGAACTTGAACACCCTTTGCCGAAAACAGCCGACCCACGAGTCCAGATTTCTGGGAATTTTGCTCCGGTACCGGAAAATCCAGTCTGTCAATCTCTTCCGGTCACCGGAAAAATACCCAAATGTGTTCAAGGCGTTTACGTTCGAAACGGAGCTAACCCTCTTTTTGAACCAACCGCCGGACACCATTTCTTCGACGGCGACGGTATGGTTCACGCCGTTCAATTCAAAAATGGGTCGGCTAGTTACGCTTGCCGTTTCACTGAAACAGAGAGGCTTGTTCAAGAAAAAGCTTTGGGTCGCCCTGTTTTCCCTAAAGCCATTGGTGAATTACATGGTCACTCTGGAATTGCAAGGCTTATGCTGTTTTACGCTCGTGGGCTCTTCGGACTTGTTGATCACAGTAAAGGAACTGGTGTTGCAAACGCCGGTTTAGTCTATTTCAATAACCGATTACTTGCTATGTCTGAAGATGATTTGCCTTACCATGTAAAGGTAACACCCACCGGCGATCTTAAAACAGAGGGTCGATTCGATTTCGACGGCCAGCTAAAATCCACCATGATAGCTCACCCAAAGCTCGACCCAGTTTCCGGTGAGCTATTTGCTCTTAGCTACGATGTGATTCAGAAGCCATACCTCAAGTACTTCAGATTTTCAAAAAATGGGGAAAAATCAAATGATGTTGAAATTCCAGTTGAAGACCCAACAATGATGCATGATTTCGCAATTACTGAGAACTTCGTCGTCATTCCTGATCAACAAGTCGTTTTCAAGATGTCTGAAATGATCCGTGGAGGTTCACCGGTGGTTTACGACAAGAACAAAGTTTCCCGATTTGGTATTCTGGATAAGTACGCGAAAGATGGGTCTGATTTGAAATGGGTTGAAGTACCTGATTGTTTCTGTTTCCACCTCTGGAATGCTTGGGAAGAAGCAGAAACAGATGAAATCGTTGTAATTGGTTCATGTATGACACCACCAGACTCCATTTTCAATGAATGTGATGAAGGGCTAAAGAGTGTTTTATCCGAAATCCGTCTCAATTTGAAAACAGGGAAATCAACAAGAAAATCCATAATCGAAAACCCGGATGAACAAGTGAATTTAGAAGCTGGAATGGTGAACCGAAACAAACTCGGAAGGAAAACAGAGTATGCTTATTTGGCTATCGCTGAACCATGGCCAAAAGTTTCTGGTTTTGCAAAAGTAAACCTGTTCACCGGTGAAGTTGAGAAATTCATTTATGGTGACAACAAATATGGTGGGGAACCTCTTTTTTTACCAAGAGACCCCAACAGCAAGGAAGAAGACGATGGTTATATTTTAGCTTTCGTTCACGATGAGAAAGAATGGAAATCAGAACTGCAAATTGTTAACGCAATGAGTTTGAAGTTGGAGGCAACTGTGAAGCTTCCATCAAGAGTTCCTTATGGATTTCATGGAACATTCATAAACGCCAATGATTTGGCAAATCAGGCATGA
CDS Sequence
  • >Solyc07g056570.1.1
    ATGGCAACTACTACTTCACATGCCACAAATACATGGATTAAGACTAAGTTGTCAATGCCATCATCAAAGGAGTTTGGTTTTGCATCAAACTCTATTTCTCTACTCAAAAATCAACATAATAGGCAAAGTCTCAACATTAATTCCTCTCTTCAAGCTCCACCTATACTTCATTTTCCTAAACAATCTTCAAATTATCAAACACCAAAGAATAATACAATTTCACACCCAAAACAAGAAAACAACAACTCCTCTTCTTCTTCAACTTCCAAGTGGAATTTAGTGCAGAAAGCAGCAGCAATGGCTTTAGATGCTGTAGAAAGTGCTTTAACTAAACATGAACTTGAACACCCTTTGCCGAAAACAGCCGACCCACGAGTCCAGATTTCTGGGAATTTTGCTCCGGTACCGGAAAATCCAGTCTGTCAATCTCTTCCGGTCACCGGAAAAATACCCAAATGTGTTCAAGGCGTTTACGTTCGAAACGGAGCTAACCCTCTTTTTGAACCAACCGCCGGACACCATTTCTTCGACGGCGACGGTATGGTTCACGCCGTTCAATTCAAAAATGGGTCGGCTAGTTACGCTTGCCGTTTCACTGAAACAGAGAGGCTTGTTCAAGAAAAAGCTTTGGGTCGCCCTGTTTTCCCTAAAGCCATTGGTGAATTACATGGTCACTCTGGAATTGCAAGGCTTATGCTGTTTTACGCTCGTGGGCTCTTCGGACTTGTTGATCACAGTAAAGGAACTGGTGTTGCAAACGCCGGTTTAGTCTATTTCAATAACCGATTACTTGCTATGTCTGAAGATGATTTGCCTTACCATGTAAAGGTAACACCCACCGGCGATCTTAAAACAGAGGGTCGATTCGATTTCGACGGCCAGCTAAAATCCACCATGATAGCTCACCCAAAGCTCGACCCAGTTTCCGGTGAGCTATTTGCTCTTAGCTACGATGTGATTCAGAAGCCATACCTCAAGTACTTCAGATTTTCAAAAAATGGGGAAAAATCAAATGATGTTGAAATTCCAGTTGAAGACCCAACAATGATGCATGATTTCGCAATTACTGAGAACTTCGTCGTCATTCCTGATCAACAAGTCGTTTTCAAGATGTCTGAAATGATCCGTGGAGGTTCACCGGTGGTTTACGACAAGAACAAAGTTTCCCGATTTGGTATTCTGGATAAGTACGCGAAAGATGGGTCTGATTTGAAATGGGTTGAAGTACCTGATTGTTTCTGTTTCCACCTCTGGAATGCTTGGGAAGAAGCAGAAACAGATGAAATCGTTGTAATTGGTTCATGTATGACACCACCAGACTCCATTTTCAATGAATGTGATGAAGGGCTAAAGAGTGTTTTATCCGAAATCCGTCTCAATTTGAAAACAGGGAAATCAACAAGAAAATCCATAATCGAAAACCCGGATGAACAAGTGAATTTAGAAGCTGGAATGGTGAACCGAAACAAACTCGGAAGGAAAACAGAGTATGCTTATTTGGCTATCGCTGAACCATGGCCAAAAGTTTCTGGTTTTGCAAAAGTAAACCTGTTCACCGGTGAAGTTGAGAAATTCATTTATGGTGACAACAAATATGGTGGGGAACCTCTTTTTTTACCAAGAGACCCCAACAGCAAGGAAGAAGACGATGGTTATATTTTAGCTTTCGTTCACGATGAGAAAGAATGGAAATCAGAACTGCAAATTGTTAACGCAATGAGTTTGAAGTTGGAGGCAACTGTGAAGCTTCCATCAAGAGTTCCTTATGGATTTCATGGAACATTCATAAACGCCAATGATTTGGCAAATCAGGCATGA
Protein Sequence
  • >Solyc07g056570.1.1
    MATTTSHATNTWIKTKLSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA*