Gene Details:

Functional Descriptions:

  • Targeted disruption of tomato chromoplast-specific lycopene β-cyclase (CYC-B) gene promotes early accumulation of lycopene in fruits and enhanced postharvest cold tolerance.
  • CYC-B knockout lines exhibited indeterminate growth and increased lycopene content in the fruit, overcoming the existing genetic linkage between these traits.
  • High-lycopene tomato genotypes containing knockout mutations within the lycopene catabolic CYC-B gene include the spontaneous old-gold (og) or old-gold crimson (ogc) mutations, and the chemically-induced mutation A949G.
  • CRISPR/Cas9 to generate knockout variants of CYC-B (Solyc06g074240) in two indeterminate elite tomato genotypes (LT16 and LT46) harbouring wild-type (WT) SP alleles

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Solyc06g074240.3.1
    ATGGAAACTCTTCTCAAGCCTTTTCCATCTCTTTTACTTTCCTCTCCTACACCCTATAGGTCTATTGTCCAACAAAATCCTTCTTTTCTAAGTCCCACCACCAAAAAAAAATCAAGAAAATGTCTTCTTAGAAACAAAAGTAGTAAACTTTTTTGTAGCTTTCTTGATTTAGCACCCACATCAAAGCCAGAGTCTTTAGATGTTAACATCTCATGGGTTGATCCTAATTCGAATCGGGCTCAATTCGACGTGATCATTATCGGAGCTGGCCCTGCTGGGCTCAGGCTAGCTGAACAAGTTTCTAAATATGGTATTAAGGTATGTTGTGTTGACCCTTCACCACTCTCCATGTGGCCAAATAATTATGGTGTTTGGGTTGATGAGTTTGAGAATTTAGGACTGGAAGATTGTTTAGATCATAAATGGCCTATGACTTGTGTGCATATAAATGATAACAAAACTAAGTATTTGGGAAGACCATATGGTAGAGTTAGTAGAAAGAAGCTGAAGTTGAAATTGTTGAATAGTTGTGTTGAGAACAGAGTGAAGTTTTATAAAGCTAAGGTTTGGAAAGTGGAACATGAAGAATTTGAGTCTTCAATTGTTTGTGATGATGGTAAGAAGATAAGAGGTAGTTTGGTTGTGGATGCAAGTGGTTTTGCTAGTGATTTTATAGAGTATGACAGGCCAAGAAACCATGGTTATCAAATTGCTCATGGGGTTTTAGTAGAAGTTGATAATCATCCATTTGATTTGGATAAAATGGTGCTTATGGATTGGAGGGATTCTCATTTGGGTAATGAGCCATATTTAAGGGTGAATAATGCTAAAGAACCAACATTCTTGTATGCAATGCCATTTGATAGAGATTTGGTTTTCTTGGAAGAGACTTCTTTGGTGAGTCGTCCTGTTTTATCGTATATGGAAGTAAAAAGAAGGATGGTGGCAAGATTAAGGCATTTGGGGATCAAAGTGAAAAGTGTTATTGAGGAAGAGAAATGTGTGATCCCTATGGGAGGACCACTTCCGCGGATTCCTCAAAATGTTATGGCTATTGGTGGGAATTCAGGGATAGTTCATCCATCAACAGGGTACATGGTGGCTAGGAGCATGGCTTTAGCACCAGTACTAGCTGAAGCCATCGTCGAGGGGCTTGGCTCAACAAGAATGATAAGAGGGTCTCAACTTTACCATAGAGTTTGGAATGGTTTGTGGCCTTTGGATAGAAGATGTGTTAGAGAATGTTATTCATTTGGGATGGAGACATTGTTGAAGCTTGATTTGAAAGGGACTAGGAGATTGTTTGACGCTTTCTTTGATCTTGATCCTAAATACTGGCAAGGGTTCCTTTCTTCAAGATTGTCTGTCAAAGAACTTGGTTTACTCAGCTTGTGTCTTTTCGGACATGGCTCAAACATGACTAGGTTGGATATTGTTACAAAATGTCCTCTTCCTTTGGTTAGACTGATTGGCAATCTAGCAATAGAGAGCCTTTGA
CDS Sequence
  • >Solyc06g074240.3.1
    ATGGAAACTCTTCTCAAGCCTTTTCCATCTCTTTTACTTTCCTCTCCTACACCCTATAGGTCTATTGTCCAACAAAATCCTTCTTTTCTAAGTCCCACCACCAAAAAAAAATCAAGAAAATGTCTTCTTAGAAACAAAAGTAGTAAACTTTTTTGTAGCTTTCTTGATTTAGCACCCACATCAAAGCCAGAGTCTTTAGATGTTAACATCTCATGGGTTGATCCTAATTCGAATCGGGCTCAATTCGACGTGATCATTATCGGAGCTGGCCCTGCTGGGCTCAGGCTAGCTGAACAAGTTTCTAAATATGGTATTAAGGTATGTTGTGTTGACCCTTCACCACTCTCCATGTGGCCAAATAATTATGGTGTTTGGGTTGATGAGTTTGAGAATTTAGGACTGGAAGATTGTTTAGATCATAAATGGCCTATGACTTGTGTGCATATAAATGATAACAAAACTAAGTATTTGGGAAGACCATATGGTAGAGTTAGTAGAAAGAAGCTGAAGTTGAAATTGTTGAATAGTTGTGTTGAGAACAGAGTGAAGTTTTATAAAGCTAAGGTTTGGAAAGTGGAACATGAAGAATTTGAGTCTTCAATTGTTTGTGATGATGGTAAGAAGATAAGAGGTAGTTTGGTTGTGGATGCAAGTGGTTTTGCTAGTGATTTTATAGAGTATGACAGGCCAAGAAACCATGGTTATCAAATTGCTCATGGGGTTTTAGTAGAAGTTGATAATCATCCATTTGATTTGGATAAAATGGTGCTTATGGATTGGAGGGATTCTCATTTGGGTAATGAGCCATATTTAAGGGTGAATAATGCTAAAGAACCAACATTCTTGTATGCAATGCCATTTGATAGAGATTTGGTTTTCTTGGAAGAGACTTCTTTGGTGAGTCGTCCTGTTTTATCGTATATGGAAGTAAAAAGAAGGATGGTGGCAAGATTAAGGCATTTGGGGATCAAAGTGAAAAGTGTTATTGAGGAAGAGAAATGTGTGATCCCTATGGGAGGACCACTTCCGCGGATTCCTCAAAATGTTATGGCTATTGGTGGGAATTCAGGGATAGTTCATCCATCAACAGGGTACATGGTGGCTAGGAGCATGGCTTTAGCACCAGTACTAGCTGAAGCCATCGTCGAGGGGCTTGGCTCAACAAGAATGATAAGAGGGTCTCAACTTTACCATAGAGTTTGGAATGGTTTGTGGCCTTTGGATAGAAGATGTGTTAGAGAATGTTATTCATTTGGGATGGAGACATTGTTGAAGCTTGATTTGAAAGGGACTAGGAGATTGTTTGACGCTTTCTTTGATCTTGATCCTAAATACTGGCAAGGGTTCCTTTCTTCAAGATTGTCTGTCAAAGAACTTGGTTTACTCAGCTTGTGTCTTTTCGGACATGGCTCAAACATGACTAGGTTGGATATTGTTACAAAATGTCCTCTTCCTTTGGTTAGACTGATTGGCAATCTAGCAATAGAGAGCCTTTGA
Protein Sequence
  • >Solyc06g074240.3.1
    METLLKPFPSLLLSSPTPYRSIVQQNPSFLSPTTKKKSRKCLLRNKSSKLFCSFLDLAPTSKPESLDVNISWVDPNSNRAQFDVIIIGAGPAGLRLAEQVSKYGIKVCCVDPSPLSMWPNNYGVWVDEFENLGLEDCLDHKWPMTCVHINDNKTKYLGRPYGRVSRKKLKLKLLNSCVENRVKFYKAKVWKVEHEEFESSIVCDDGKKIRGSLVVDASGFASDFIEYDRPRNHGYQIAHGVLVEVDNHPFDLDKMVLMDWRDSHLGNEPYLRVNNAKEPTFLYAMPFDRDLVFLEETSLVSRPVLSYMEVKRRMVARLRHLGIKVKSVIEEEKCVIPMGGPLPRIPQNVMAIGGNSGIVHPSTGYMVARSMALAPVLAEAIVEGLGSTRMIRGSQLYHRVWNGLWPLDRRCVRECYSFGMETLLKLDLKGTRRLFDAFFDLDPKYWQGFLSSRLSVKELGLLSLCLFGHGSNMTRLDIVTKCPLPLVRLIGNLAIESL*