Information report for g08490
Gene Details
Functional Annotation
- Ubiquitin-specific protease, which together with UBP12 deubiquitinates DA1, DAR1 and DAR2, hence reducing their peptidase activity. Works upstream of DA1, DAR1 and DAR2 to restrict their protease activity and hence fine-tune plant growth and development.
- Encodes a homolog of the mammalian protein CstF77, a polyadenylation factor subunit. RNA 3′end鈥損rocessing factor of antisense FLC transcript. Mediates silencing of the floral repressor gene FLC.
- Encodes calcium dependent protein kinase 6 (CPK6), a member of the Arabidopsis CDPK gene family. CDPKs contain an intrinsic Ca2+-activation domain with four EF hand Ca2+-binding sites. CDPKs protein kinases have been proposed to function in multiple plant signal transduction pathways downstream of [Ca2+]cyt elevations, thus transducing various physiological responses. CPK6 is expressed in both guard cells and mesophyll cells. Functions in guard cell ion channel regulation. ABA and Ca(2+) activation of slow-type anion channels and, interestingly, ABA activation of plasma membrane Ca(2+)-permeable channels were impaired in independent alleles of single and double cpk3cpk6 mutant guard cells. Furthermore, ABA- and Ca(2+)-induced stomatal closing were partially impaired in these cpk3cpk6 mutant alleles. The protein kinase CPK6 is shown in biochemical assays to be directly activated by elevations in calcium concentrations in the physiological range (Laanements et al., 2013 PlantPhys.; PMID: 23766366). These data correlate with the in vivo function of CPK6 in Ca2+ and ABA activation of S-type anion channels (Mori et al., 2006 PLoS Biol.; PMID: 17032064) and the ability of CPK6 to mediate ABA activation of SLAC1 (Brandt et al., 2012 PNAS; PMID: 22689970).
Homologous
- Arabidopsis thaliana: AT3G11910
- Brassica napus: BnaC09G0582400ZS, BnaA10G0266500ZS, GSBRNA2T00111112001, BnaC05G0483000ZS, BnaA05G0429700ZS, BnaC02G0024600ZS, BnaA02G0022900ZS, BnaA03G0024600ZS, BnaC03G0029800ZS
- Brassica oleracea: Bo9g176000, Bo5g135050, Bo2g007280
- Brassica rapa: Bra009210, Bra028727, Bra005900
Sequences
gDNA Sequence
- >g08490
TATACTAGTCAGTCACTTGAATTGACTGGCAACGTTTTACATTGTCCACCTATCAGCATTCAGCAAGGTCAACAATAATCCTATTGCCAAATGTGGGAAACGAGGTTTGAAACGGGAACATTTTACATTTTCCTTCACTTGTTCTATCCTGTTGTTGTAGTTGCTGCAGACGAGAATGAAGGGATCGGGTATGCAAGTTTCAACAGAGCATCGCTAAAATTGGATCTTAGATCATCAACTCCTTGCATTCAGGTTCCCAACAAAACAAAAGGTATATCTTTTCTAAGCTATAATCTATCGGTTTAGATCTCTCTGTTTTGTATAGACATTTAGGAAAAGCTTCCCTTTGAAACTGCTCTTAAACTTTGATGTTCTCTCTTGTGACCTTTTTTATCGAGACTTAAGTATTTCACTGTATTTTGCACTCAGTGTGAAAGTACTACGAGGAAGAAACTCTTCTCAATGGCTTCTGACAATCATGATTTTCCTCAGCCTATGGAAGGTAAGGTTTTCGTGTTTAATTGACAATTCGATCTGGTTATTCTATTCTCGTCTGTGTTCTACTTTGATTGGTTTGATGTAGATGCTTTAGCACTTGTTGATAGTGTTGGGTTGTAGAATTCATGTTTTCACAGTGCGCTAGGGTTTTGTCAATTTTTGGGTTGTGGATTTCTTAACGATTGTTGATGCATTTGTAGTTGCCCAAACTGAGCCTGCTGCTACCGCGGTGGAGAATCCGCCACCCGAGGATCCTCCAACTCTGAAATTCACGTGGACCATCCCAATTTTCACTAGGCTCAATACCAGGAAGCATTATTCTGATGTATTTGTATGTTGGCGGTTATAAATGGTAATGGCGTATACACTTTTTGATGCGCTTTTTTGCAATTGATATCCTGATGAGCATTTTACTGGTTGACTGATTTTTTTCTCTTGCAGGCGTTTAATGATTTTTCCCAAAGGAAACAATGTCGACCATTTGTCAATGTACTTGGATGTTGCTGACGGCCTAAATTTGCCGTATGGCTATGAGCAGATATTCACAGTTCTGTCTGGCTGTAGTGAACCAAGTCAACAGCCGATATTCCATCAGAAAGGGTATCTTAGTTTATTAATTTTGTTCACACATATATAAAATTAGGAGTTACAGCAGATATTCACAGTTCTGTCTGGCTGTAGTGAACCAAGTCAACTGCATCTATTAATTTTGTTCACACATATAAATTTCTGAATTTCAGATGTTTATTTCTTGGAGTTATAGTTTTGGATTTGTAAAGGGAGTATGTGGTAGACTTTGAAATGGAGTCAAGCTACCGCTTTCTTAAACATTGACCATGCCATACCTGAAAACTTTATTGAAAATGATTAATTATTAATATGTGTTTTACTCTTTTGAACTTTCTAAACAGCCAATGACTTTTGTTATCTGGTAAGTAGATGCAGTTTTTACTGAGTTAACTATCTTTGCGCGTGGCTGCAGGACGCAACATCAGCTCAATGCAAGAGAAAGCGATTGGGGGCTTACATCATTCATGCCTCTCAGGGGGCTTTATGATATATTTAGTGAATGACACTGTTCTGATTGAAGCTGAAGTTGCTGTCAAAAAAAGAGATTGGTTTTGGTGGACTAAAAAACCAAGGTGCTACCTGTTGCATGAATTCTCTCCTGCAGACTTTATACAGCATACCTTACTTCAGAAAGGTTGGATGCATGTCTTGTTTTCTTTTTCTATTTGCTTCATCATGAGTTCACTAAATTCTAGAGAAAGACATCTGAATAATGCATATTACTAGTTGATTTTTTTTTTATGTGTTTAGTTATCTGCTCTAAGAGATATTACTAGTTGGGATTATTCTTGTATTAGAGCAACAACTGTTATAGTTTCAGATAAGCACTTCTTATTTAGATATGGCATCTTGAATTGGAAAATATGAGGATACAAAATACCATTTTTTTTGCTCTGGTGTGCAGTTAGAAAGTACCCCACGATAGCCCTGTCAAACTGTGATGATTCCTGTCTTCGTATCAAATTCCTTGGAAACGTTTAATGGTTGTTTGGGAAGGTTTGGTGGTAAGTAACTAACTTATTTAGTTTGTTATTTTGTAGCTGGTGCGTTTAACCAGAAAACTTTTCGTACACTAGAAAAACCAAAAGAAGACGAGTTTACTATGGAGCTGTAAGTACCTTTATGGACAACTTTGTTTCGTTGATTACAGTATTGGAATTCGTAATGCTTCTACTTTCAGGTCAAAGCTGCACACTTATGATGATGTTGACGATAGAGTGGCTGAGAAGCTTGGCCTTGATGATCCATCGAAACAGAGACTTACATCTCACAATACCTTTGACTCCCAGCAACCCAAGCCTCAGCCAATCAAATACCGTGGAGTAGATCATCTTTCAGATATGTTAGTTCACTATAATCAGGTGATTGACATTGGTTTGAGTGAACTTACATATTCTTTTTTTATATTATTATTCTAATGCATAAATATTGCATATTATGAATTTTTCTTCTTTTTGCAGACGTCTGACATTTTGTATTATGAAGTTCTGGATATTCCTCTTCCAGAATTGCAAGGTCCTAAGACTCTAAAAGTGGCTTGTATACCTTGTTGTAGTTACATCGTGATTGACTTGACTGCTACAAATATCAATCTGTGTGGAAAAACAATTCCTCGTAGTTGGCATCAAATTATACACGGACAGACATGTACAATATTTAAGAATAACCGGTTGACAAAAAAAAAAAAAAAAAGAATAACCGAGAAGAACAAAAGAAATAAAATCCAGCAAAATAATTTTTAATTTTTTTTTGACAAAATTTAAAGTATCAAACTGAAAGTGAGGAGGCTTTACAGAATAAAAGAAAAGGTTTTTTTCAGCAGAAAAAAAGAAGAAGAAATTTAATTTCCAAGATTTTCAATATCCAGTAGGGCTCTTTAGAGTAGAGATACTTTGGTTACCTTTTAGAGATTAGATAAAAAGTCTTTGTTCAAAGTATATTTTTCTTGAAACAAAGAGGGAAATTAAAACGCCAATTAGAAAAGATTCATTTCTACAAACATCAATTTTGTATATGGAAATCATTTGAAATCAGAAAATGAGTTTTTGACTTTTGACTGAAGGAAAAACATACAAAGAATATAAAATTTGTGAATATAGAAAGCAATGTAATATTTACCATAAAAAGTTTTATGCATGTACGAATCCTCATTACCACATGTACCTTACCTATCATCCTATAAATTTTCTCTCTTGATGTTTTCACTTTAACGTGAAAGAAGAAGAAGATAAAATTGTTCTTTCTTCGATAATCACCCACAAGAATATGTGTATTTCTTGGCTAAATGGCAAGAAAAATGGATATGTGGCTTCAATTAAGAAGTGGACTTATTCAGGGTATTACAAGACTAGTGGATGCTTCTCAATCGGGAAATATAGCAGCAACGGCTCTCTCAACTCATTCTCACATAGAGTCCAACGGACAATATGTGAGAAAGGATTGGTGCACATATATCCGAGAAGAGGCAATATTTGGGCTCTTTATGAAAACTGGTCACCATCTTGGGATATTTCAACTTCAGTAGAAGAAATGAACAAGTATGAAATGGTAGAAGTCCTTAAAGATTTCGACGAAGAGAGAGGTGTGACAGTTGTGCCGCTTGTTAAACTCTCAGGATTTAAACTTTAATTTTAAGTCACAACCGTCACACATCCTTCTTCATCAAAATCTTGAAGCACTTCAACCACTTCATACTTGTCCACAACTTCTTGGGGGAGTTGAACTGTCCCAAGATGTTGATCAGTTT
CDS Sequence
- >Ma01_g08490
ATGTGGGAAACGAGGTTTGAAACGGGAACATTTTACATTTTCCTTCACTTGTTCTATCCTGTTGTTGTAGTTGCTGCAGACGAGAATGAAGGGATCGGGTATGCAAGTTTCAACAGAGCATCGCTAAAATTGGATCTTAGATCATCAACTCCTTGCATTCAGTGTGAAAGTACTACGAGGAAGAAACTCTTCTCAATGGCTTCTGACAATCATGATTTTCCTCAGCCTATGGAAGTTGCCCAAACTGAGCCTGCTGCTACCGCGGTGGAGAATCCGCCACCCGAGGATCCTCCAACTCTGAAATTCACGTGGACCATCCCAATTTTCACTAGGCTCAATACCAGGAAGCATTATTCTGATGTATTTGTATGTTGGCGGCGTTTAATGATTTTTCCCAAAGGAAACAATGTCGACCATTTGTCAATGTACTTGGATGTTGCTGACGGCCTAAATTTGCCGTATGGCTATGAGCAGATATTCACAGTTCTGTCTGGCTGTAGTGAACCAAGTCAACAGCCGATATTCCATCAGAAAGGGACGCAACATCAGCTCAATGCAAGAGAAAGCGATTGGGGGCTTACATCATTCATGCCTCTCAGGGGGCTTTATGATATATTTACTGGTGCGTTTAACCAGAAAACTTTTCGTACACTAGAAAAACCAAAAGAAGACGAGTTTACTATGGAGCTGTCAAAGCTGCACACTTATGATGATGTTGACGATAGAGTGGCTGAGAAGCTTGGCCTTGATGATCCATCGAAACAGAGACTTACATCTCACAATACCTTTGACTCCCAGCAACCCAAGCCTCAGCCAATCAAATACCGTGGAGTAGATCATCTTTCAGATATGTTAGTTCACTATAATCAGACGTCTGACATTTTGTATTATGAAGTTCTGGATATTCCTCTTCCAGAATTGCAAGGGTATTACAAGACTAGTGGATGCTTCTCAATCGGGAAATATAGCAGCAACGGCTCTCTCAACTCATTCTCACATAGAGTCCAACGGACAATATGTGAGAAAGGATTGGTGCACATATATCCGAGAAGAGGCAATATTTGGGCTCTTTATGAAAACTGGTCACCATCTTGGGATATTTCAACTTCAGTAGAAGAAATGAACAAGTATGAAATGGTAGAAGTCCTTAAAGATTTCGACGAAGAGAGAGGTGTGACAGTTGTGCCGCTTGTTAAACTCTCAGGATTTAAACTTTAA
Protein Sequence
- >Ma01_g08490
MWETRFETGTFYIFLHLFYPVVVVAADENEGIGYASFNRASLKLDLRSSTPCIQCESTTRKKLFSMASDNHDFPQPMEVAQTEPAATAVENPPPEDPPTLKFTWTIPIFTRLNTRKHYSDVFVCWRRLMIFPKGNNVDHLSMYLDVADGLNLPYGYEQIFTVLSGCSEPSQQPIFHQKGTQHQLNARESDWGLTSFMPLRGLYDIFTGAFNQKTFRTLEKPKEDEFTMELSKLHTYDDVDDRVAEKLGLDDPSKQRLTSHNTFDSQQPKPQPIKYRGVDHLSDMLVHYNQTSDILYYEVLDIPLPELQGYYKTSGCFSIGKYSSNGSLNSFSHRVQRTICEKGLVHIYPRRGNIWALYENWSPSWDISTSVEEMNKYEMVEVLKDFDEERGVTVVPLVKLSGFKL