Gene Details:

• MSU gene ID: None
• RAPdb gene ID: None
• Gene Symbol: OsPUL
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• The expression of the OsPUL gene was maintained at a significant level during the entire grain filling period and in the late stage of seed germination, which coincided with its involvement in starch anabolism and catabolism.
• The OsPUL gene maintained a relatively high expression level during the entire period of seed development, and peaked in the middle and late stages.
• Characterization of expression of the OsPUL gene encoding a pullulanase-type debranching enzyme during seed development and germination in rice.
• In addition, the 1177-bp OsPUL promoter sequence was cloned, and some endosperm-specific motifs such as the GCN4 and AACA motifs were observed to exist in this region.
• The data from both histochemical and fluorometric analyses showed that the OsPUL promoter was capable of driving the target gene to have a high level of endosperm-specific expression.
• Furthermore, the seed germination experiment showed that the OsPUL promoter actively functions in the late stage of seed germination.
• In the present study, we performed a quantitative real-time PCR (Q-PCR) analysis of the expression pattern of the OsPUL gene encoding a pullulanase-type DBE in different tissues as well as in seeds at different developmental stages.

Function-related keywords:

• starch ,  grain-filling ,  seed-development ,  branching ,  endosperm ,  seed-germination ,  seed ,  grain

Literature:

• Characterization of expression of the OsPUL gene encoding a pullulanase-type debranching enzyme during seed development and germination in rice .  DOI:  10.1016/j.plaphy.2009.02.001 ;  PMID:  19251430
• Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1-isoamylase2 hetero-oligomer from rice endosperm .  DOI:  10.1007/s00425-006-0331-z ;  PMID:  16953433
• Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis .  DOI:  10.1093/pcp/pcr058 ;  PMID:  21551159
• Sequence variations in OsAGPase significantly associated with amylose content and viscosity properties in rice (Oryza sativa L.) .  DOI:  10.1017/S0016672312000390 ;  PMID:  22950899

Related News:

Gene Resources:

• NCBI ID: AB012915
• UniProt accessions:

Sequences: