Information report for OsmiR535
Gene Details
Functional Descriptions
- The multiple roles of OsmiR535 in modulating plant height, panicle branching and grain shape.
- Furthermore, OsmiR535 overexpression imposed great influence in panicle architecture, such as more but shorter panicles, and fewer primary/secondary panicle branches.
- Through quantitative real-time PCR analyses, we further revealed that OsmiR535 overexpression repressed the expression of OsSPL7/12/16, as well as the OsSPLs downstream panicle related genes, including OsPIN1B, OsDEP1, OsLOG and OsSLR1.
- Taken together, our findings suggest that OsmiR535 multiply modulates plant height, panicle architecture and grain shape possibly by regulating OsSPLs genes in rice.
- Moreover, OsmiR535 overexpression increased the grain length, but did not affect grain width.
- Expectedly, OsmiR535 overexpression in rice reduced plant height by decreasing the 1st and 2nd internode length.
- In addition, the overexpression of OsmiR535 significantly reduced the survival rate of rice seedlings during PEG and dehydration post-stress recovery.
- OsmiR535 is involved in regulating the cold-stress response, modulating plant development, and determining panicle architecture and grain length.
- However, the role that OsmiR535 plays in plant responses to drought and salinity are elusive.
- Moreover, our practical application of CRISPR/Cas9 mediated genome editing created a homozygous 5 bp deletion in the coding sequence of OsmiR535, demonstrating that OsmiR535 could be a useful genetic editing target for drought and salinity tolerance and a new marker for molecular breeding of Oryza sativa.
- With transgenic and CRISPR/Cas9 knockout system techniques, our results verified that either inhibition or knockout of OsmiR535 in rice could enhance the tolerance of plants to NaCl, ABA, dehydration and PEG stresses.
- In the current study, molecular and genetic engineering techniques were used to elucidate the possible role of OsmiR535 in response to NaCl, PEG(Poly ethylene glycol), ABA(Abscisic acid), and dehydration stresses.
- Our results demonstrated that OsmiR535 negatively regulates the stress response in rice.
- Our results showed that OsmiR535 is induced under stressed conditions as compared to control.
Functional Keywords
- panicle , grain , grain-length , architecture , branching , height , plant-height , internode-length , panicle-architecture , grain-width , seedlings , plant-development , drought , salinity , tolerance , ethylene , stress , breeding , abscisic-acid , stress-response , ER-stress
Literature and News
Gene Resources
Sequences
cDNA Sequence
- >LOC_Os12g44380.3
TGTGGTCTTTGTTATGCCTTTGCATTCTATGGGAAACACCTGATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAAGAATATTGCTACATATAAATTGTCGGCCATTCTTTGCAATTCGACTCATAAGAGGCACTCGGAACGCTATGCAGTGCATGGGGGAATTGTATATTATCTCCGAATCAAGAAGGGGATAATGCTTGCTTTCTCCATGAGCTATTTTTGCCTTTTTCATGCCGGATCATCATATGCTGTCGTACATTGGATGATCTTATGCTGTTGTACATTGGATGTTGGTCATTTGTAGAGATACTAGTGAATAAAAGTTGCAGGAGTTGGTTCACTCGAGAAAATTCTGGTCAGTATGTCGTCCATCTGCTGCACGACAGCAGTTAGGAGCCGAATAGCATGTCCATGGGTTTTCATCAAATGTTGTATCATCATTTGTTTTTTGATACGTTCAGACGGCTTCAGTGCTGTGTGAATATATATGTATGGAATATATCGAGAAAATGTTGTTGTAACATTTGGTTTTTGATCGTTGAGACGA - >LOC_Os12g44380.2
TGTGGTCTTTGTTATGCCTTTGCATTCTATGGGAAACACCTGATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAAGAATATTGCTACATATAAATTGTCGGCCATTCTTTGCAATTCGACTCATAAGAGGCACTCGGAACGCTATGCAGTGCATGGGGGAATTGTATATTATCTCCGAATCAAGAAGGGGATAATGCTTGCTTTCTCCATGAGCTATTTTTGCCTTTTTCATGCCGGATCATCATATGCTGTCGTACATTGGATGATCTTATGCTGTTGTACATTGGATGTTGGTCATTTGTAGAGATACTAGTGAATAAAAGTTGCAGGAGTTGGTTCACTCGAGAAAATTCTGGTCAGTATGTCGTCCATCTGCTGCACGACAGCAGTTAGGAGCCGAATAGCATGTCCATGGGTTTTCATCAAATGTTGTATCATCATTTGTTTTTTGATACGTTCAGACGGCTTCAGTGCTGTGTGAATATATATGTATGGAATATATCGAGAAAATGTTGTTGTAACATTTGGTTTTTGATCGTTGAGACGA - >LOC_Os12g44380.1
TGTGGTCTTTGTTATGCCTTTGCATTCTATGGGAAACACCTGATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAAGAATATTGCTACATATAAATTGTCGGCCATTCTTTGCAATTCGACTCATAAGAGGCACTCGGAACGCTATGCAGTGCATGGGGGAATTGTATATTATCTCCGAATCAAGAAGGGGATAATGCTTGCTTTCTCCATGAGCTATTTTTGCCTTTTTCATGCCGGATCATCATATGCTGTCGTACATTGGATGATCTTATGCTGTTGTACATTGGATGTTGGTCATTTGTAGAGATACTAGTGAATAAAAGTTGCAGGAGTTGGTTCACTCGAGAAAATTCTGGTCAGTATGTCGTCCATCTGCTGCACGACAGCAGTTAGGAGCCGAATAGCATGTCCATGGGTTTTCATCAAATGTTGTATCATCATTTGTTTTTTGATACGTTCAGACGGCTTCAGTGCTGTGTGAATATATATGTATGGAATATATCGAGAAAATGTTGTTGTAACATTTGGTTTTTGATCGTTGAGACGA
CDS Sequence
- >LOC_Os12g44380.3
ATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAA - >LOC_Os12g44380.2
ATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAA - >LOC_Os12g44380.1
ATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAA
Protein Sequence
- >LOC_Os12g44380.3
MQTLHSEGMIVLFVMEMLEFHENDPRRTRIANAYFSLFMALGNILGYATGAYSGWYKIFPFTVTPSCSISCANLKSAFLLDIIILVVTTCITVASVQEPQSFGSDEADHPSTEQEAFLWELFGSFRYFTLPVWMVLIVTALTWIGWFPFILFDTDWMGREIYRGSPDDPSITQSYHDGVRMGSFGLMLNSVLLGFTSIVLEKLCRKWGAGLVWGVSNILMALCFVAMLVITYVAKNMDYPPSGVPPTGIVIASLVVFTILGAPLAITYSIPYAMAASRVENLGLGQGLAMGILNLAIVIPQVIVSLGSGPWDQLFGGGNAPAFAVAAAASFIGGLVAILGLPRARIASRRRGHR* - >LOC_Os12g44380.2
MQTLHSEGMIVLFVMEMLEFHENDPRRTRIANAYFSLFMALGNILGYATGAYSGWYKIFPFTVTPSCSISCANLKSAFLLDIIILVVTTCITVASVQEPQSFGSDEADHPSTEQEAFLWELFGSFRYFTLPVWMVLIVTALTWIGWFPFILFDTDWMGREIYRGSPDDPSITQSYHDGVRMGSFGLMLNSVLLGFTSIVLEKLCRKWGAGLVWGVSNILMALCFVAMLVITYVAKNMDYPPSGVPPTGIVIASLVVFTILGAPLAITYSIPYAMAASRVENLGLGQGLAMGILNLAIVIPQVIVSLGSGPWDQLFGGGNAPAFAVAAAASFIGGLVAILGLPRARIASRRRGHR* - >LOC_Os12g44380.1
MQTLHSEGMIVLFVMEMLEFHENDPRRTRIANAYFSLFMALGNILGYATGAYSGWYKIFPFTVTPSCSISCANLKSAFLLDIIILVVTTCITVASVQEPQSFGSDEADHPSTEQEAFLWELFGSFRYFTLPVWMVLIVTALTWIGWFPFILFDTDWMGREIYRGSPDDPSITQSYHDGVRMGSFGLMLNSVLLGFTSIVLEKLCRKWGAGLVWGVSNILMALCFVAMLVITYVAKNMDYPPSGVPPTGIVIASLVVFTILGAPLAITYSIPYAMAASRVENLGLGQGLAMGILNLAIVIPQVIVSLGSGPWDQLFGGGNAPAFAVAAAASFIGGLVAILGLPRARIASRRRGHR*