Gene Details:

• MSU gene ID: None
• RAPdb gene ID: None
• Gene Symbol: OsmiR535
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• The multiple roles of OsmiR535 in modulating plant height, panicle branching and grain shape.
• Furthermore, OsmiR535 overexpression imposed great influence in panicle architecture, such as more but shorter panicles, and fewer primary/secondary panicle branches.
• Through quantitative real-time PCR analyses, we further revealed that OsmiR535 overexpression repressed the expression of OsSPL7/12/16, as well as the OsSPLs downstream panicle related genes, including OsPIN1B, OsDEP1, OsLOG and OsSLR1.
• Taken together, our findings suggest that OsmiR535 multiply modulates plant height, panicle architecture and grain shape possibly by regulating OsSPLs genes in rice.
• Moreover, OsmiR535 overexpression increased the grain length, but did not affect grain width.
• Expectedly, OsmiR535 overexpression in rice reduced plant height by decreasing the 1st and 2nd internode length.
• In addition, the overexpression of OsmiR535 significantly reduced the survival rate of rice seedlings during PEG and dehydration post-stress recovery.
• OsmiR535 is involved in regulating the cold-stress response, modulating plant development, and determining panicle architecture and grain length.
• However, the role that OsmiR535 plays in plant responses to drought and salinity are elusive.
• Moreover, our practical application of CRISPR/Cas9 mediated genome editing created a homozygous 5 bp deletion in the coding sequence of OsmiR535, demonstrating that OsmiR535 could be a useful genetic editing target for drought and salinity tolerance and a new marker for molecular breeding of Oryza sativa.
• With transgenic and CRISPR/Cas9 knockout system techniques, our results verified that either inhibition or knockout of OsmiR535 in rice could enhance the tolerance of plants to NaCl, ABA, dehydration and PEG stresses.
• In the current study, molecular and genetic engineering techniques were used to elucidate the possible role of OsmiR535 in response to NaCl, PEG(Poly ethylene glycol), ABA(Abscisic acid), and dehydration stresses.
• Our results demonstrated that OsmiR535 negatively regulates the stress response in rice.
• Our results showed that OsmiR535 is induced under stressed conditions as compared to control.

Function-related keywords:

• panicle ,  grain ,  grain-length ,  architecture ,  branching ,  height ,  plant-height ,  internode-length ,  panicle-architecture ,  grain-width ,  seedlings ,  plant-development ,  drought ,  salinity ,  tolerance ,  ethylene ,  stress ,  breeding ,  abscisic-acid ,  stress-response ,  ER-stress

Literature:

• The multiple roles of OsmiR535 in modulating plant height, panicle branching and grain shape .  DOI:  10.1016/j.plantsci.2019.02.002 ;  PMID:  31128716
• OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa .  DOI:  10.3390/plants9101337 ;  PMID:  33050518

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: