Information report for OsHRZ1
Gene Details
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Functional Descriptions
- Here we report the identification of new iron regulators in rice, designated Oryza sativa Haemerythrin motif-containing Really Interesting New Gene (RING)- and Zinc-finger protein 1 (OsHRZ1) and OsHRZ2.
- OsHRZ1, OsHRZ2 and their Arabidopsis homologue BRUTUS bind iron and zinc, and possess ubiquitination activity.
- OsHRZ1 and OsHRZ2 are susceptible to degradation in roots irrespective of iron conditions.
- These results suggest that OsHRZ1 and OsHRZ2 are iron-binding sensors that negatively regulate iron acquisition under conditions of iron sufficiency.
- Many jasmonate-inducible genes were also negatively regulated by the ubiquitin ligases OsHRZ1 and OsHRZ2 and positively regulated by the transcription factor IDEF1.
- C1 (OE) rice plants modify the expression of the putative iron-sensing factors OsHRZ1 and OsHRZ2 and enhance the expression of OsIRO2 under copper excess, which suggests a role of copper transport in iron signaling.
- Transgenic rice plants with repressed expression of OsHRZ1 and OsHRZ2 (HRZ knockdown lines) are tolerant to low iron availability and accumulate iron in shoots and seeds under both iron-sufficient and -deficient conditions without a growth penalty.
- Two iron-binding ubiquitin ligases, OsHRZ1 and OsHRZ2, negatively regulate such iron deficiency responses in rice (Oryza sativa).
Literature and News
- Iron-binding haemerythrin RING ubiquitin ligases regulate plant iron responses and accumulation . DOI: 10.1038/ncomms3792 ; PMID: 24253678
- Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots . DOI: 10.1007/s11103-016-0486-3 ; PMID: 27143046
- Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice (Oryza sativa L.) . DOI: 10.1007/s11103-017-0622-8 ; PMID: 28631167
- Rice HRZ ubiquitin ligases are crucial for response to excess iron . DOI: 10.1111/ppl.12698 ; PMID: 29655221
- Simultaneous Enhancement of iron Deficiency Tolerance and Iron Accumulation in Rice by Combining the Knockdown of OsHRZ Ubiquitin Ligases with the Introduction of Engineered Ferric-chelate Reductase . DOI: 10.1186/s12284-022-00598-w ; PMID: 36315339
Gene Resources
Sequences
cDNA Sequence
- >LOC_Os12g44380.3
TGTGGTCTTTGTTATGCCTTTGCATTCTATGGGAAACACCTGATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAAGAATATTGCTACATATAAATTGTCGGCCATTCTTTGCAATTCGACTCATAAGAGGCACTCGGAACGCTATGCAGTGCATGGGGGAATTGTATATTATCTCCGAATCAAGAAGGGGATAATGCTTGCTTTCTCCATGAGCTATTTTTGCCTTTTTCATGCCGGATCATCATATGCTGTCGTACATTGGATGATCTTATGCTGTTGTACATTGGATGTTGGTCATTTGTAGAGATACTAGTGAATAAAAGTTGCAGGAGTTGGTTCACTCGAGAAAATTCTGGTCAGTATGTCGTCCATCTGCTGCACGACAGCAGTTAGGAGCCGAATAGCATGTCCATGGGTTTTCATCAAATGTTGTATCATCATTTGTTTTTTGATACGTTCAGACGGCTTCAGTGCTGTGTGAATATATATGTATGGAATATATCGAGAAAATGTTGTTGTAACATTTGGTTTTTGATCGTTGAGACGA - >LOC_Os12g44380.2
TGTGGTCTTTGTTATGCCTTTGCATTCTATGGGAAACACCTGATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAAGAATATTGCTACATATAAATTGTCGGCCATTCTTTGCAATTCGACTCATAAGAGGCACTCGGAACGCTATGCAGTGCATGGGGGAATTGTATATTATCTCCGAATCAAGAAGGGGATAATGCTTGCTTTCTCCATGAGCTATTTTTGCCTTTTTCATGCCGGATCATCATATGCTGTCGTACATTGGATGATCTTATGCTGTTGTACATTGGATGTTGGTCATTTGTAGAGATACTAGTGAATAAAAGTTGCAGGAGTTGGTTCACTCGAGAAAATTCTGGTCAGTATGTCGTCCATCTGCTGCACGACAGCAGTTAGGAGCCGAATAGCATGTCCATGGGTTTTCATCAAATGTTGTATCATCATTTGTTTTTTGATACGTTCAGACGGCTTCAGTGCTGTGTGAATATATATGTATGGAATATATCGAGAAAATGTTGTTGTAACATTTGGTTTTTGATCGTTGAGACGA - >LOC_Os12g44380.1
TGTGGTCTTTGTTATGCCTTTGCATTCTATGGGAAACACCTGATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAAGAATATTGCTACATATAAATTGTCGGCCATTCTTTGCAATTCGACTCATAAGAGGCACTCGGAACGCTATGCAGTGCATGGGGGAATTGTATATTATCTCCGAATCAAGAAGGGGATAATGCTTGCTTTCTCCATGAGCTATTTTTGCCTTTTTCATGCCGGATCATCATATGCTGTCGTACATTGGATGATCTTATGCTGTTGTACATTGGATGTTGGTCATTTGTAGAGATACTAGTGAATAAAAGTTGCAGGAGTTGGTTCACTCGAGAAAATTCTGGTCAGTATGTCGTCCATCTGCTGCACGACAGCAGTTAGGAGCCGAATAGCATGTCCATGGGTTTTCATCAAATGTTGTATCATCATTTGTTTTTTGATACGTTCAGACGGCTTCAGTGCTGTGTGAATATATATGTATGGAATATATCGAGAAAATGTTGTTGTAACATTTGGTTTTTGATCGTTGAGACGA
CDS Sequence
- >LOC_Os12g44380.3
ATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAA - >LOC_Os12g44380.2
ATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAA - >LOC_Os12g44380.1
ATGCAAACATTACATTCAGAAGGCATGATTGTGCTTTTTGTCATGGAAATGCTGGAGTTCCACGAGAATGACCCAAGGAGGACTCGGATAGCTAATGCTTACTTCTCATTGTTCATGGCCCTGGGAAACATACTTGGATATGCCACTGGAGCATACAGTGGCTGGTACAAGATATTCCCGTTCACCGTTACTCCATCATGTAGCATCAGCTGTGCCAACCTCAAGTCTGCCTTTCTACTTGATATTATCATTTTGGTGGTCACTACATGCATCACTGTAGCATCAGTGCAAGAGCCTCAATCCTTTGGAAGTGATGAAGCAGATCACCCTAGCACAGAACAGGAAGCTTTCCTCTGGGAACTTTTTGGATCATTCCGGTACTTTACATTACCGGTTTGGATGGTTTTGATTGTTACTGCCCTCACATGGATTGGATGGTTTCCATTTATCCTCTTTGATACCGATTGGATGGGTCGAGAGATCTATCGTGGAAGTCCAGATGATCCAAGTATAACTCAGAGCTATCATGATGGTGTGAGAATGGGTTCTTTTGGTCTGATGCTGAACTCGGTCCTTCTTGGATTCACTTCTATTGTACTAGAGAAGTTATGTCGGAAGTGGGGAGCTGGACTGGTGTGGGGTGTCTCCAATATCCTAATGGCATTGTGCTTTGTGGCAATGCTTGTAATAACATATGTGGCAAAGAATATGGATTATCCACCTAGTGGAGTACCACCAACCGGCATTGTCATTGCTTCCCTGGTAGTTTTTACAATTTTAGGAGCGCCCCTGGCGATCACGTACAGTATACCATATGCAATGGCTGCTAGTCGGGTTGAAAATCTGGGACTTGGCCAAGGTCTAGCAATGGGCATTCTTAATTTGGCTATTGTCATACCACAGGTTATTGTGTCACTGGGTAGCGGGCCCTGGGACCAACTGTTTGGTGGTGGCAATGCACCAGCCTTTGCAGTGGCTGCTGCTGCATCTTTTATCGGTGGGCTGGTGGCTATTCTGGGCCTTCCACGAGCCCGCATTGCATCAAGGAGGAGAGGTCACCGATAA
Protein Sequence
- >LOC_Os12g44380.3
MQTLHSEGMIVLFVMEMLEFHENDPRRTRIANAYFSLFMALGNILGYATGAYSGWYKIFPFTVTPSCSISCANLKSAFLLDIIILVVTTCITVASVQEPQSFGSDEADHPSTEQEAFLWELFGSFRYFTLPVWMVLIVTALTWIGWFPFILFDTDWMGREIYRGSPDDPSITQSYHDGVRMGSFGLMLNSVLLGFTSIVLEKLCRKWGAGLVWGVSNILMALCFVAMLVITYVAKNMDYPPSGVPPTGIVIASLVVFTILGAPLAITYSIPYAMAASRVENLGLGQGLAMGILNLAIVIPQVIVSLGSGPWDQLFGGGNAPAFAVAAAASFIGGLVAILGLPRARIASRRRGHR* - >LOC_Os12g44380.2
MQTLHSEGMIVLFVMEMLEFHENDPRRTRIANAYFSLFMALGNILGYATGAYSGWYKIFPFTVTPSCSISCANLKSAFLLDIIILVVTTCITVASVQEPQSFGSDEADHPSTEQEAFLWELFGSFRYFTLPVWMVLIVTALTWIGWFPFILFDTDWMGREIYRGSPDDPSITQSYHDGVRMGSFGLMLNSVLLGFTSIVLEKLCRKWGAGLVWGVSNILMALCFVAMLVITYVAKNMDYPPSGVPPTGIVIASLVVFTILGAPLAITYSIPYAMAASRVENLGLGQGLAMGILNLAIVIPQVIVSLGSGPWDQLFGGGNAPAFAVAAAASFIGGLVAILGLPRARIASRRRGHR* - >LOC_Os12g44380.1
MQTLHSEGMIVLFVMEMLEFHENDPRRTRIANAYFSLFMALGNILGYATGAYSGWYKIFPFTVTPSCSISCANLKSAFLLDIIILVVTTCITVASVQEPQSFGSDEADHPSTEQEAFLWELFGSFRYFTLPVWMVLIVTALTWIGWFPFILFDTDWMGREIYRGSPDDPSITQSYHDGVRMGSFGLMLNSVLLGFTSIVLEKLCRKWGAGLVWGVSNILMALCFVAMLVITYVAKNMDYPPSGVPPTGIVIASLVVFTILGAPLAITYSIPYAMAASRVENLGLGQGLAMGILNLAIVIPQVIVSLGSGPWDQLFGGGNAPAFAVAAAASFIGGLVAILGLPRARIASRRRGHR*