Gene Details:

• MSU gene ID: LOC_Os12g42420
• RAPdb gene ID: Os12g0618800
• Gene Symbol: OsPLS3 OsGCNT
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence.
• OsPLS3 regulates the onset of leaf senescence in rice.
• Through map-based cloning strategy, we determined that 22-bp deletion in the OsPLS3 gene encoding a domain of unknown function 266 (DUF266)-containing protein, a member of GT14-like, underlies the premature leaf senescence phenotype in the OsPLS3 mutant.
• The OsPLS3 mRNA levels progressively declined with the age-dependent leaf senescence in wild-type rice, implying a negative role of OsPLS3 in regulating leaf senescence.
• Our results indicated that the DUF266-containing gene OsPLS3 plays an important role in the onset of leaf senescence, in part through cytokinin and ethylene signaling in rice.
• Key message The OsPLS3 locus was isolated by map-based cloning that encodes a DUF266-containing protein.
• Physiological analysis, and histochemical staining and transmission electron microscopy assays indicated that the OsPLS3 mutant accumulated higher levels of ethylene and reactive oxygen species than its wild type.
• Compared to the wild type IR64, the spotted-leaf mutant spl21 exhibited loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence associated genes, which indicated that OsGCNT regulates premature leaf senescence.
• High throughput RNA sequencing analysis provided further evidence for the biological effects of loss of OsGCNT function on cell death, premature leaf senescence and enhanced disease resistance in rice.
• Thus, we demonstrated that the novel OsGCNT regulated rice innate immunity and immunity-associated leaf senescence probably by changing the jasmonate metabolic pathway.
• Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice.
• oryzae, up-regulation of pathogenesis-related genes and increased level of jasmonate which suggested that OsGCNT is a negative regulator of defense response in rice.

Function-related keywords:

• leaf ,  leaf-senescence ,  senescence ,  map-based-cloning ,  ethylene ,  cytokinin ,  reactive-oxygen-species ,  resistance ,  defense ,  jasmonate ,  defense-response ,  disease ,  disease-resistance ,  cell-death ,  immunity ,  innate-immunity

Literature:

• A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence .  DOI:  10.1007/s11103-018-0758-1 ;  PMID:  30117035
• Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice .  DOI:  10.1186/s12870-018-1489-9 ;  PMID:  30382816
• Mutation in a putative glycosyltransferase-like gene causes programmed cell death and early leaf senescence in rice .  DOI:  10.1186/s12284-019-0266-1 ;  PMID:  30758674

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: