Information report for OsPLS3;OsGCNT
Gene Details
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Functional Descriptions
- A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence.
- OsPLS3 regulates the onset of leaf senescence in rice.
- Through map-based cloning strategy, we determined that 22-bp deletion in the OsPLS3 gene encoding a domain of unknown function 266 (DUF266)-containing protein, a member of GT14-like, underlies the premature leaf senescence phenotype in the OsPLS3 mutant.
- The OsPLS3 mRNA levels progressively declined with the age-dependent leaf senescence in wild-type rice, implying a negative role of OsPLS3 in regulating leaf senescence.
- Our results indicated that the DUF266-containing gene OsPLS3 plays an important role in the onset of leaf senescence, in part through cytokinin and ethylene signaling in rice.
- Key message The OsPLS3 locus was isolated by map-based cloning that encodes a DUF266-containing protein.
- Physiological analysis, and histochemical staining and transmission electron microscopy assays indicated that the OsPLS3 mutant accumulated higher levels of ethylene and reactive oxygen species than its wild type.
- Compared to the wild type IR64, the spotted-leaf mutant spl21 exhibited loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence associated genes, which indicated that OsGCNT regulates premature leaf senescence.
- High throughput RNA sequencing analysis provided further evidence for the biological effects of loss of OsGCNT function on cell death, premature leaf senescence and enhanced disease resistance in rice.
- Thus, we demonstrated that the novel OsGCNT regulated rice innate immunity and immunity-associated leaf senescence probably by changing the jasmonate metabolic pathway.
- Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice.
- oryzae, up-regulation of pathogenesis-related genes and increased level of jasmonate which suggested that OsGCNT is a negative regulator of defense response in rice.
Functional Keywords
Literature and News
- A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence . DOI: 10.1007/s11103-018-0758-1 ; PMID: 30117035
- Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice . DOI: 10.1186/s12870-018-1489-9 ; PMID: 30382816
- Mutation in a putative glycosyltransferase-like gene causes programmed cell death and early leaf senescence in rice . DOI: 10.1186/s12284-019-0266-1 ; PMID: 30758674
Gene Resources
- UniProt: Q2QM36
- EMBL: AK243349, AP014968, DP000011
- AlphaFoldDB: Q2QM36
- EnsemblPlants: Os12t0618800-01
- Gramene: Os12t0618800-01
- KEGG: osa:4352812
- Orthologous matrix: KGCTHFT
- InterPro: IPR003406, IPR044174
- PANTHER: PTHR31042, PTHR31042:SF151
- OrthoDB: Q2QM36
- SWISS-MODEL: Q2QM36
- eggNOG: ENOG502QV1G
Sequences
cDNA Sequence
- >LOC_Os12g42420.1
TCCTCTCCTCTCCTCTCCTCTCCTCTTCTTCTCCCTCCAAAAATCTCCTCCTCCGCTTCACTCGCTCCCCTTCCCTCCATTCCATTCCCTTCCTCTTCGCCCGCCGCGAGATCGGTAGACCAAATTAACCACACTCGCGCCGCCATTCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAGTTGAGCTGCTTGTCCCGAGCTCTGCTCGTTTCTTGGCGTCTTCTTTCTTCTTCTTCCTCCTCGGTGCGGTTGCGGTTTCGTTTTCGTTTTCTTTCCCCTTTTTCTGTCGGTTAATTCGGCGACGTCTGATCCGCCATGAGAGCGAGCAGTTTGTTCACTCAGTTCCCCTTCGTCTGATACTGATCCCTCCTCACTCTCTGCTCGATTTCTCATCGTCTCTGTAAGCAAGATCCGATCCCCGGAGGCCAAAACCCCTCCCGAATCGCGTGGTTCTTGTTTGTTCTAATCATCCTTCGATTGGGTTGGGTTGAGCAATCGATTGCGTTGTTGTTCTTGCTGATCCGAAGATCCAAAGGAGGAGGAGGAGCTTGAGGTTGAAATTTCTCGCCGGAGATGGCGCTGCCGCACGCCGCCTCGCCGTACGTGCTGTCGCTGCTGCTGCTGCTCTCCATCCCGGCGGTGTTCCTCCTCGCGCCGCGCCTCCTCCCGCCCAAGACGCTCCCCTCCATCCCCGACGCCGACGAGACCGACGACCTCGCCCTCTTCCGCCGCGCCGTCCTCCTCTCCGCCGCCCCGGACTCCTCCTCCGCCTCCGCCGGCGCCGCCTCCCTCTTCGGGCGGCGGCCGCAGCCCAAGGTGGCGTTCCTCTTCCTGACCAACTCCGACCTCGTCTTCTCCCCGCTCTGGGAGAAGTACTTCGCCGGGAACCACCACCTCCTCAACCTCTACATCCACGCCGACCCCAGCGCCGCCGTCGACCTCCCCGCCACCGCCTCCTTCCGCGGCCACGTCATCCGCGGCACCAAGGCGACGGCGCGCGCCTCGGCCACGCTCATCTCCGCCGCGCGCCGCCTCCTCGCCACCGCCCTCCTCGACGACCCATCGAACCACTTCTTCGCCCTCCTCTCCCAGTCCTGCATCCCCCTCCACCCTTTCCCCACCTTCTACCGCACCCTCCTCTCCGACAGCGACAACAATGGCGGCTCGCCTCGCCGCCCCCGCCGCCGCCGCAGCTTCATCGAGATCCTCGACAACGAGCCGACGCTCCACGACCGCTACTACGCCCGCGGCGACGACGTGATGCTACCCGAGGTGCCCTACGACAGCTTCCGCGTCGGCTCGCAGTTCTTCGTGCTCGTGCGCCGCCACGCCGTCATGGTGGTGAGGGATCGGAGGCTGTGGAACAAGTTCAAGCTGCCATGCCTGACCAAGAGGAAGGACTCGTGCTACCCGGAGGAGCACTACTTCCCGACGCTGCTCGACATGCAGGATCCGCAGGGATGCACCAAGTTCACGCTCACCAGGGTCAATTGGACCGACTCCGTCGACGGCCACCCGCACACCTACCGCCCCGACGAGGTCTCCGGCGAGCTCATCAGGGAGCTCAGGAAGTCCAATGGGACTCACTCCTACATGTTCGCGCGCAAATTCGCGCCCGATTGCCTCAAGCCACTGATGGAGATCGCCGACTCCGTCATCCTGCGAGACTAGATGAATGATGCTGTGTTTGCAAGAGATGAATGCAATGAGGGCTGAGATGTGATGAACAAGCATAAGCTGATCCATCCATACCCTTGCAGATTCTCCAGAGAGTACAAACCAGGACCAAGCCTTGGTTTGCTTTGTTTTCTTCAACTGCACTCGTCAAAAAGATGGTGCTTCTCGAGAGAGCATGATGGTTGGTGTTGGTGGCAAAAGGGCCCCAGCTGCCTCCTATGTGAGTATTTTTCTCTGTATCTTTTGTTTTCCCTTTCGAATTTGAGCTCAAGTGGTGGCCAAAATGAGGAGGGGAGAAGGTTGTTGGCACGTAATTAGAGTAGTAGTTAGCATGATCATCCTATGTATTACATGGAGGAAAATCTGTCGATACATGCAGTGATGGTTTGTAGTGTATGTTTATATACAATATGTCAATATCAAGCCCTTTTTTTCGTCCGAAATGTATCGTTTGTATCATGGAAAACCTA
CDS Sequence
- >LOC_Os12g42420.1
ATGGCGCTGCCGCACGCCGCCTCGCCGTACGTGCTGTCGCTGCTGCTGCTGCTCTCCATCCCGGCGGTGTTCCTCCTCGCGCCGCGCCTCCTCCCGCCCAAGACGCTCCCCTCCATCCCCGACGCCGACGAGACCGACGACCTCGCCCTCTTCCGCCGCGCCGTCCTCCTCTCCGCCGCCCCGGACTCCTCCTCCGCCTCCGCCGGCGCCGCCTCCCTCTTCGGGCGGCGGCCGCAGCCCAAGGTGGCGTTCCTCTTCCTGACCAACTCCGACCTCGTCTTCTCCCCGCTCTGGGAGAAGTACTTCGCCGGGAACCACCACCTCCTCAACCTCTACATCCACGCCGACCCCAGCGCCGCCGTCGACCTCCCCGCCACCGCCTCCTTCCGCGGCCACGTCATCCGCGGCACCAAGGCGACGGCGCGCGCCTCGGCCACGCTCATCTCCGCCGCGCGCCGCCTCCTCGCCACCGCCCTCCTCGACGACCCATCGAACCACTTCTTCGCCCTCCTCTCCCAGTCCTGCATCCCCCTCCACCCTTTCCCCACCTTCTACCGCACCCTCCTCTCCGACAGCGACAACAATGGCGGCTCGCCTCGCCGCCCCCGCCGCCGCCGCAGCTTCATCGAGATCCTCGACAACGAGCCGACGCTCCACGACCGCTACTACGCCCGCGGCGACGACGTGATGCTACCCGAGGTGCCCTACGACAGCTTCCGCGTCGGCTCGCAGTTCTTCGTGCTCGTGCGCCGCCACGCCGTCATGGTGGTGAGGGATCGGAGGCTGTGGAACAAGTTCAAGCTGCCATGCCTGACCAAGAGGAAGGACTCGTGCTACCCGGAGGAGCACTACTTCCCGACGCTGCTCGACATGCAGGATCCGCAGGGATGCACCAAGTTCACGCTCACCAGGGTCAATTGGACCGACTCCGTCGACGGCCACCCGCACACCTACCGCCCCGACGAGGTCTCCGGCGAGCTCATCAGGGAGCTCAGGAAGTCCAATGGGACTCACTCCTACATGTTCGCGCGCAAATTCGCGCCCGATTGCCTCAAGCCACTGATGGAGATCGCCGACTCCGTCATCCTGCGAGACTAG
Protein Sequence
- >LOC_Os12g42420.1
MALPHAASPYVLSLLLLLSIPAVFLLAPRLLPPKTLPSIPDADETDDLALFRRAVLLSAAPDSSSASAGAASLFGRRPQPKVAFLFLTNSDLVFSPLWEKYFAGNHHLLNLYIHADPSAAVDLPATASFRGHVIRGTKATARASATLISAARRLLATALLDDPSNHFFALLSQSCIPLHPFPTFYRTLLSDSDNNGGSPRRPRRRRSFIEILDNEPTLHDRYYARGDDVMLPEVPYDSFRVGSQFFVLVRRHAVMVVRDRRLWNKFKLPCLTKRKDSCYPEEHYFPTLLDMQDPQGCTKFTLTRVNWTDSVDGHPHTYRPDEVSGELIRELRKSNGTHSYMFARKFAPDCLKPLMEIADSVILRD*