Information report for SAPK9;OsSAPK9
Gene Details
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Functional Descriptions
- We have shown recently that three members of the rice SnRK2 protein kinase family, SAPK8, SAPK9 and SAPK10, are activated by ABA signal as well as by hyperosmotic stress.
- Stress-Activated Protein Kinase OsSAPK9 Regulates Tolerance to Salt Stress and Resistance to Bacterial Blight in Rice.
- Moreover, in vivo and in vitro experiments demonstrated that OsSAPK9 forms a protein complex with the molecular chaperones OsSGT1 and OsHsp90, and transgenic plants overexpressing OsSGT1 exhibited decreased tolerances to salt stress and significantly increased resistance levels to bacterial blight.
- This study confirms that OsSAPK9 functions as a positive regulator of salt-stress responses and disease resistance through its interaction with OsSGT1 in rice.
- Thus, OsSAPK9 may function as a center node regulator of salt-stress responses and disease-resistance pathways through its interaction with OsSGT1 in rice.
- When OsSAPK9 or OsbZIP20 are knocked out in rice, ABA-mediated antioxidant and GS/GOGAT activity enhancement under high-NH4+ stress disappear, and the two mutants are more sensitive to high-NH4+ stress compared with their wild types.
- Using RNA sequencing and quantitative real-time PCR approaches, we ascertain that two genes, OsSAPK9 and OsbZIP20, are induced both by high NH4+ and by ABA.
Functional Keywords
- ABA , resistance , disease , disease-resistance , blight , bacterial-blight , salt , tolerance , salt-stress , stress , Kinase , protein-kinase , stress-response , node
Literature and News
- Abscisic acid-activated SNRK2 protein kinases function in the gene-regulation pathway of ABA signal transduction by phosphorylating ABA response element-binding factors . DOI: 10.1111/j.1365-313X.2005.02583.x ; PMID: 16359387
- Differential activation of the rice sucrose nonfermenting1-related protein kinase2 family by hyperosmotic stress and abscisic acid . DOI: 10.1105/tpc.019943 ; PMID: 15084714
- Stress-Activated Protein Kinase OsSAPK9 Regulates Tolerance to Salt Stress and Resistance to Bacterial Blight in Rice . DOI: 10.1186/s12284-019-0338-2 ; PMID: 31712918
- Endogenous ABA alleviates rice ammonium toxicity by reducing ROS and free ammonium via regulation of the SAPK9-bZIP20 pathway . DOI: 10.1093/jxb/eraa076 ; PMID: 32064504
- Rice OsPUB16 modulates the ‘SAPK9-OsMADS23-OsAOC’ pathway to reduce plant water-deficit tolerance by repressing ABA and JA biosynthesis . DOI: 10.1371/journal.pgen.1010520 ; PMID: 36441771
Gene Resources
- UniProt: Q75V57
- EMBL: AB125310, AK069697, AP008218
- AlphaFoldDB: Q75V57
- EnsemblPlants: Os12t0586100-01
- Gramene: Os12t0586100-01
- KEGG: dosa:Os12g0586100
- Orthologous matrix: MQPKAWG
- InterPro: IPR000719, IPR008271, IPR011009
- PANTHER: PTHR24343, PTHR24343:SF326
- SUPFAM: SSF56112
- PROSITE: PS00107, PS00108, PS50011
- Gene3D: 1.10.510.10, 3.30.200.20
- OrthoDB: Q75V57
- SWISS-MODEL: Q75V57
- Conserved Domain Database: cd14662
- eggNOG: KOG0583
Homologs
- Panicum virgatum: Pavir.9NG208400
- Setaria italica: Seita.9G169200
- Setaria viridis: SEVIR_9G167300v2
Sequences
cDNA Sequence
- >LOC_Os12g39630.1
GCGGGAGCAAAAGGAGAAAAAAAAACAACAGCGAGAAAAAAAAAATCAAGAACACGAGCAAGCGAGAGGGGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCACGAGATCGGAGGAGGAGGAGGAGGAAGAAGAGGGGAGGAGGTGGCGTGTTGAGTCGGATCGAGGTTTGCGTTTGTGTTCGTTTGGTCGCCGGCGGAAGCGATGGAGAGGGCGGCGGCGGGGCCGCTGGGGATGGAGATGCCGATAATGCACGACGGTGACAGGTACGAGCTGGTGAAGGAGATCGGGTCGGGGAACTTCGGCGTCGCCCGCCTCATGCGCAACCGCGCCTCCGGCGACCTCGTCGCCGTCAAGTACATCGACCGCGGCGAGAAGATTGACGAGAACGTGCAGAGGGAGATCATCAACCACAGGTCGCTGCGCCACCCCAACATCATCCGATTCAAGGAGGTTATTCTGACGCCGACGCATCTCGCGATCGTCATGGAGTACGCCTCCGGCGGCGAGCTCTTCGAGCGCATCTGCAGCGCCGGCCGCTTCAGCGAGGACGAGGCTCGTTTCTTCTTCCAGCAGCTGATATCTGGAGTTAGCTACTGCCATTCCATGCAAGTATGCCATCGTGACTTAAAGCTGGAGAACACTCTGCTAGATGGAAGTACTGCTCCTCGCTTGAAGATATGTGACTTTGGTTACTCGAAGTCATCGGTTCTTCATTCACAACCAAAATCAACAGTTGGAACTCCAGCTTATATTGCTCCAGAAGTTTTGCTCAAGAAAGAATACGATGGAAAGATTGCCGATGTTTGGTCATGCGGTGTGACGCTCTACGTGATGTTGGTTGGCGCATACCCTTTCGAGGATCCTGAAGATCCCAAGAACTTCAGAAAGACAATTCAGAAAATATTGGGTGTTCAGTACTCAATTCCAGACTATGTCCACATATCTCCGGAGTGCCGCGATCTCATTACGAGGATTTTTGTTGGCAACCCAGCTAGTAGGATCACCATGCCTGAGATAAAGAACCACCCATGGTTCATGAAGAACATCCCGGCTGACCTCATGGATGATGGCATGGTTAGCAATCAGTACGAGGAGCCTGACCAGCCGATGCAGAATATGAACGAGATCATGCAGATACTGGCAGAAGCAACAATTCCAGCAGCAGGCACCAGTGGAATCAACCAGTTCTTGACTGACAGCCTTGACCTCGACGACGACATGGAGGATATGGACTCGGACCTTGACCTTGACATTGAGAGCAGCGGAGAGATCGTATATGCCATGTAATCATCTCAACATTTGCAATGAAACTGCACTGAAAATCAGCTTGTTTCAGAGCAGGTAGAGCTCCAGAAGAGATCTGTGTTCAGGCTACTAGGAAGCAGGCTCTGCTGCCTATTAGTTCTTGAGTCTGAATTTCTGATGAACCATACTGCAGCTGAGTTGCAGCAGATTGTGTAGTATGTAATTATGTATGTTGTGCCAAAAATTTAGCACCGGATGATATCATGCAAGACTGTTTCTTGGGAACACTGAGAAATGAGAAGCATTGTATGGAACCTAGTTCTGTATTGTATTAAACAGAGAGACAGAAGAGATGTATTGGTTAATGTATTTGCTATGTGTATGTAAATATGCTTTGTGCCAAGAAGAAAAAATCTCTATTAATTCTATGGTTCTATTTTCTCAGCAAGCTTTGGCTGCTGCTGGAGAGTCAGTTGCATGAACACTGAACATGTCAAAATTGCAGAGTGGATTGTTCCTCCATCCTGTATTAGACAGGGAAAAATATACACATCATCTGTACAAAGGTGAGCTTGGAGCCAAAAGCATCCAGGAACATCAGTCTACAAGCCAGTCCAGGTTATAACACTCTTCTGCTACCTGAACCCGGAGGTCTGGGATGACCTGCTCAGCTTCCCTCCACAGCAACGCCGTCTCCTCGTCGCACACGACGTGCCCGAGGGACTTGAGCGATGAGGACGACCTCGGGAGGCTCCTCAGTCGCGAGCATTCTCGCATGTCGATCTTCTCCAGGCTTGTCAGGTGACCGAGCTCCTCAGGGAGGTCCGTCAGGTTGACACACTGCGAAATGTCGAGGTATTTCAGCCTCTTCAGGCTGCATACCGAAGGCGGGAGCCGCCACAGAGCCGGGCAGGCGTATACCCTTAGGATGCTCAGGCAATGCAGCTTGCCCAGCTCATATGGAAGCTCTGTGAGGTCATGGCAGTTTGAGATGGAGATGCTCTCCAGGGAACTGATTTCACATATGCTAGATGGTAGCTCCTTCAGGTCTATGCAGTGGTCAATGGTGAGGTTGGATAGGCGCGGGAATGTCATCGAGAGATCCACCTTTGATCCTCTCAGACTGTTGGTCAGCTCACAGAGGACAAGGGATATCTTGCGCAGGTTCTTCAGTGGGATTGTGGTTTTTGGTAGCGGTGGAAGTGTGATCTTCTCAAGCCAAAGGCTCCTCAGGTCACTGAGCGTGGTGAAGGCGGATAGGTTATCAAGGGTTGCACTGATGGTGCCATAGTTGATCAGCACCAGGGCTTTCAAGTTCTGCATGGTTGCAATGAATGGTGGGAGATAGTATACACTTGACGCGAAGTTGAGGATAAGCACTTCGGCCTTGGGGAAGCTCATCTGGAACCAGTCAGATTCTTTCATCTCAC
CDS Sequence
- >LOC_Os12g39630.1
ATGGAGAGGGCGGCGGCGGGGCCGCTGGGGATGGAGATGCCGATAATGCACGACGGTGACAGGTACGAGCTGGTGAAGGAGATCGGGTCGGGGAACTTCGGCGTCGCCCGCCTCATGCGCAACCGCGCCTCCGGCGACCTCGTCGCCGTCAAGTACATCGACCGCGGCGAGAAGATTGACGAGAACGTGCAGAGGGAGATCATCAACCACAGGTCGCTGCGCCACCCCAACATCATCCGATTCAAGGAGGTTATTCTGACGCCGACGCATCTCGCGATCGTCATGGAGTACGCCTCCGGCGGCGAGCTCTTCGAGCGCATCTGCAGCGCCGGCCGCTTCAGCGAGGACGAGGCTCGTTTCTTCTTCCAGCAGCTGATATCTGGAGTTAGCTACTGCCATTCCATGCAAGTATGCCATCGTGACTTAAAGCTGGAGAACACTCTGCTAGATGGAAGTACTGCTCCTCGCTTGAAGATATGTGACTTTGGTTACTCGAAGTCATCGGTTCTTCATTCACAACCAAAATCAACAGTTGGAACTCCAGCTTATATTGCTCCAGAAGTTTTGCTCAAGAAAGAATACGATGGAAAGATTGCCGATGTTTGGTCATGCGGTGTGACGCTCTACGTGATGTTGGTTGGCGCATACCCTTTCGAGGATCCTGAAGATCCCAAGAACTTCAGAAAGACAATTCAGAAAATATTGGGTGTTCAGTACTCAATTCCAGACTATGTCCACATATCTCCGGAGTGCCGCGATCTCATTACGAGGATTTTTGTTGGCAACCCAGCTAGTAGGATCACCATGCCTGAGATAAAGAACCACCCATGGTTCATGAAGAACATCCCGGCTGACCTCATGGATGATGGCATGGTTAGCAATCAGTACGAGGAGCCTGACCAGCCGATGCAGAATATGAACGAGATCATGCAGATACTGGCAGAAGCAACAATTCCAGCAGCAGGCACCAGTGGAATCAACCAGTTCTTGACTGACAGCCTTGACCTCGACGACGACATGGAGGATATGGACTCGGACCTTGACCTTGACATTGAGAGCAGCGGAGAGATCGTATATGCCATGTAA
Protein Sequence
- >LOC_Os12g39630.1
MERAAAGPLGMEMPIMHDGDRYELVKEIGSGNFGVARLMRNRASGDLVAVKYIDRGEKIDENVQREIINHRSLRHPNIIRFKEVILTPTHLAIVMEYASGGELFERICSAGRFSEDEARFFFQQLISGVSYCHSMQVCHRDLKLENTLLDGSTAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLLKKEYDGKIADVWSCGVTLYVMLVGAYPFEDPEDPKNFRKTIQKILGVQYSIPDYVHISPECRDLITRIFVGNPASRITMPEIKNHPWFMKNIPADLMDDGMVSNQYEEPDQPMQNMNEIMQILAEATIPAAGTSGINQFLTDSLDLDDDMEDMDSDLDLDIESSGEIVYAM*