Information report for SPL11
Gene Details
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Functional Descriptions
- Expression analysis indicated that SPL11 is induced in both incompatible and compatible rice-blast interactions.
- The rice (Oryza sativa) E3 ligase SPOTTED LEAF11 (SPL11) negatively regulates programmed cell death and disease resistance.
- The rice U-box/ARM E3 ubiquitin ligase SPL11 negatively regulates programmed cell death (PCD) and disease resistance, and controls flowering time through interacting with the novel RNA/DNA binding KH domain protein SPIN1.
- These results suggest that RBS1 is a new negative regulator of flowering time that itself is positively regulated by SPIN1 but negatively regulated by SPL11 in rice.
- SPIN1, a K homology domain protein negatively regulated and ubiquitinated by the E3 ubiquitin ligase SPL11, is involved in flowering time control in rice.
- In vitro ubiquitination assay indicated that the SPL11 protein possesses E3 ubiquitin ligase activity that is dependent on an intact U-box domain, suggesting a role of the ubiquitination system in the control of plant cell death and defense.
- We found that spl2, spl7 and SPL11 mutant cells accumulated increased amounts of H(2)O(2) in response to rice blast fungal elicitor.
- We demonstrate here that SPL11 also regulates flowering via interaction with SPIN1 (for SPL11-interacting protein1), a Signal Transduction and Activation of RNA family member.
- SPL11 mutants have delayed flowering under long-day conditions.
- Our data are consistent with a model in which SPIN1 acts as a negative regulator of flowering that itself is negatively regulated by SPL11, possibly via ubiquitination.
- The rice (Oryza sativa) spotted leaf11 (SPL11) mutant was identified from an ethyl methanesulfonate-mutagenized indica cultivar IR68 population and was previously shown to display a spontaneous cell death phenotype and enhanced resistance to rice fungal and bacterial pathogens.
Functional Keywords
- blast , disease , disease-resistance , flowering-time , defense , leaf , flower , cell-death
Literature and News
- RBS1, an RNA binding protein, interacts with SPIN1 and is involved in flowering time control in rice . DOI: 10.1371/journal.pone.0087258 ; PMID: 24498057
- Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-box/armadillo repeat protein endowed with E3 ubiquitin ligase activity . DOI: 10.1105/tpc.104.025171 ; PMID: 15377756
- Regulatory mechanisms of ROI generation are affected by rice spl mutations . DOI: 10.1093/pcp/pcj074 ; PMID: 16816407
- SPIN1, a K homology domain protein negatively regulated and ubiquitinated by the E3 ubiquitin ligase SPL11, is involved in flowering time control in rice . DOI: 10.1105/tpc.108.058610 ; PMID: 18586868
Gene Resources
- NCBI ID: AY652590
Sequences
cDNA Sequence
- >LOC_Os12g38210.1
CCCCACTATTTACCATTCTGCCACTAGCCGAGGTCTCCTCCTCCTCTTCTTCTTCCCGCTGCTTCGCCTCGCGCTCGCCATGGCCGGCGACCGAGCGGAGGAGGAGGAGGGGGAGGCGCCGCCTCCGGAGGCGCGTGCCGCGGCGGCGGTGGAGAGGGTGGCGGCGGATTCGGCTGCTGGGGCCCTTCGTGGAGGAGCTCCGGGAGCGGAGGCGGGGGGAGGGGGAGGGGGAGGAGGAGGAGCGGGCGCTGGCGCCGCTCGCCGACGCGCTCGAGGCGGCGCTCGCGCTGCTCCGGCTCGGCCGCGAGGGGAGCAGGATCTCCTTGGTTCTTGAGAGGGACAGCGTAATGAAAAAGTTTCAGGGAGTAATTTTGCAGCTGGAACAAGCTCTGTGCGACATTCCGTACAATGAATTGGATATATCAGATGAAGTTAGAGAGCAGGTTGAGTTAGTGCACGCACAGCTCAAAAGAGCAAAAGAACGGATTGATATGCCTGACGATGAATTCTACAATGATCTACTATCTGTATATGACAAGAACTATGACCCAAGTGCAGAACTGGCTATTCTTGGAAGATTGTCAGAAAAGCTACACTTGATGACAATCACCGATCTCACGCAAGAATCACTCGCCTTACATGAGATGGTCGCATCCGGCGGTGGTCAGGACCCAGGAGAACACATTGAGAGAATGTCAATGCTACTGAAGAAGATTAAGGATTTTGTGCAAACACAGAACCCTGACATGGGGCCTCCTATGGCTTCCAGGGTATTGGATTCAAATGGGGATTCAAGGCCTATAACCATTCCTGACGAGTTCCGATGTCCAATTTCTCTTGAGCTGATGAAGGATCCTGTTATAGTGTCTACAGGGCAGACATATGAGCGGGCATGCATTGAGAAATGGATAGCATCAGGCCATCATACCTGCCCAACCACGCAACAGAAGATGTCAACCTCAGCATTGACGCCAAACTATGTCCTTAGAAGTCTCATTTCCCAGTGGTGTGAAACCAATGGGATGGAGCCACCAAAGCGCTCAACCCAGCCTAACAAGCCTACGCCTGCATGCTCTTCGAGTGAACGTGCTAACATCGATGCTCTGCTATCTAAGTTATGCTCTCCAGACACTGAGGAGCAGAGATCAGCTGCTGCAGAGCTTCGCCTCCTTGCAAAGCGGAATGCAAACAACCGGATTTGCATTGCTGAGGCAGGTGCCATTCCCTTGCTATTGAGTCTGCTGTCCTCATCTGACCTACGGACTCAAGAACATGCAGTTACTGCCCTTCTGAACCTCTCCATACATGAGGACAATAAGGCAAGCATCATATCATCTGGTGCTGTCCCAAGCATAGTCCATGTTCTGAAGAATGGCAGCATGGAGGCACGGGAGAATGCTGCGGCCACACTTTTTAGTCTCTCAGTGATTGATGAATATAAAGTGACAATTGGAGGGATGGGGGCTATCCCTGCGCTTGTAGTGCTGTTAGGCGAGGGTAGCCAGCGGGGGAAGAAGGATGCAGCAGCAGCTCTCTTCAACTTGTGTATATACCAAGGGAACAAGGGACGAGCGATTCGAGCTGGCCTTGTGCCACTCATCATGGGGCTGGTGACCAACCCCACAGGAGCGCTCATGGATGAGGCTATGGCAATACTCTCCATACTATCCAGCCACCCTGAGGGCAAGGCAGCGATTGGGGCGGCAGAGCCTGTCCCTGTGCTTGTGGAGATGATCGGAAGCGGAACCCCGAGAAATCGAGAGAATGCTGCGGCTGTCATGCTACATCTGTGCAGTGGAGAGCACCATCTTGTGCATTTAGCTCGTGCGCAGGAGTGCGGGATCATGGTCCCGTTGCGGGAGCTGGCTTTGAACGGCACCGATAGGGGGAAGAGGAAGGCTGTGCAGCTGCTCGAGCGGATGAGCAGATTCCTAGTCCAGCAACAAGAGGAACAGGAGTCCCAGTCGCAAGCCTCCGCGCAGGTCCCTCCTCAGGCCACCCCTGAGCAGGTCCCGGAAAATGACATCCCTGAACAATTGGACAGCCCAGCGTCTCAATACCCTATGGTTGTATGAACTCGATGATGAGCGAGTCATCGTCGCAATCCTATCAAATGTCGGATTGAGTAGCATCCTGGTCACTTTGCATTCTGCTCATGATTCATGGCATGCCGACCAATTGAGGTTTTTCCACAAGCTTGAACTATAAGGTTTCCATGCTTGTGTGCTGAAACTCCCAGGTTGTGAGAATTTGTTTAGGCAGCCCAGGATGTTGAGATAGATGTGTACAGTTTCTTAAACATGCAGCTGCTATATGTAGACAACATAATGCAAGGTTTTCCCATGTATATCCTGAAGACTGTAATACCATATGTAAAAGAGAAGTTTCAAATGCGGATTTGAGCAGTAAATCTGATGGCTTTACTACTAGTAACTACTCCCTCTGTCCAAAAAGACTCAATTCCTGAGTTTTTTTGTCTAACGTTTGATTGTTCGTCTTATATGAATTTTTTTTTATAA
CDS Sequence
- >LOC_Os12g38210.1
ATGAAAAAGTTTCAGGGAGTAATTTTGCAGCTGGAACAAGCTCTGTGCGACATTCCGTACAATGAATTGGATATATCAGATGAAGTTAGAGAGCAGGTTGAGTTAGTGCACGCACAGCTCAAAAGAGCAAAAGAACGGATTGATATGCCTGACGATGAATTCTACAATGATCTACTATCTGTATATGACAAGAACTATGACCCAAGTGCAGAACTGGCTATTCTTGGAAGATTGTCAGAAAAGCTACACTTGATGACAATCACCGATCTCACGCAAGAATCACTCGCCTTACATGAGATGGTCGCATCCGGCGGTGGTCAGGACCCAGGAGAACACATTGAGAGAATGTCAATGCTACTGAAGAAGATTAAGGATTTTGTGCAAACACAGAACCCTGACATGGGGCCTCCTATGGCTTCCAGGGTATTGGATTCAAATGGGGATTCAAGGCCTATAACCATTCCTGACGAGTTCCGATGTCCAATTTCTCTTGAGCTGATGAAGGATCCTGTTATAGTGTCTACAGGGCAGACATATGAGCGGGCATGCATTGAGAAATGGATAGCATCAGGCCATCATACCTGCCCAACCACGCAACAGAAGATGTCAACCTCAGCATTGACGCCAAACTATGTCCTTAGAAGTCTCATTTCCCAGTGGTGTGAAACCAATGGGATGGAGCCACCAAAGCGCTCAACCCAGCCTAACAAGCCTACGCCTGCATGCTCTTCGAGTGAACGTGCTAACATCGATGCTCTGCTATCTAAGTTATGCTCTCCAGACACTGAGGAGCAGAGATCAGCTGCTGCAGAGCTTCGCCTCCTTGCAAAGCGGAATGCAAACAACCGGATTTGCATTGCTGAGGCAGGTGCCATTCCCTTGCTATTGAGTCTGCTGTCCTCATCTGACCTACGGACTCAAGAACATGCAGTTACTGCCCTTCTGAACCTCTCCATACATGAGGACAATAAGGCAAGCATCATATCATCTGGTGCTGTCCCAAGCATAGTCCATGTTCTGAAGAATGGCAGCATGGAGGCACGGGAGAATGCTGCGGCCACACTTTTTAGTCTCTCAGTGATTGATGAATATAAAGTGACAATTGGAGGGATGGGGGCTATCCCTGCGCTTGTAGTGCTGTTAGGCGAGGGTAGCCAGCGGGGGAAGAAGGATGCAGCAGCAGCTCTCTTCAACTTGTGTATATACCAAGGGAACAAGGGACGAGCGATTCGAGCTGGCCTTGTGCCACTCATCATGGGGCTGGTGACCAACCCCACAGGAGCGCTCATGGATGAGGCTATGGCAATACTCTCCATACTATCCAGCCACCCTGAGGGCAAGGCAGCGATTGGGGCGGCAGAGCCTGTCCCTGTGCTTGTGGAGATGATCGGAAGCGGAACCCCGAGAAATCGAGAGAATGCTGCGGCTGTCATGCTACATCTGTGCAGTGGAGAGCACCATCTTGTGCATTTAGCTCGTGCGCAGGAGTGCGGGATCATGGTCCCGTTGCGGGAGCTGGCTTTGAACGGCACCGATAGGGGGAAGAGGAAGGCTGTGCAGCTGCTCGAGCGGATGAGCAGATTCCTAGTCCAGCAACAAGAGGAACAGGAGTCCCAGTCGCAAGCCTCCGCGCAGGTCCCTCCTCAGGCCACCCCTGAGCAGGTCCCGGAAAATGACATCCCTGAACAATTGGACAGCCCAGCGTCTCAATACCCTATGGTTGTATGA
Protein Sequence
- >LOC_Os12g38210.1
MKKFQGVILQLEQALCDIPYNELDISDEVREQVELVHAQLKRAKERIDMPDDEFYNDLLSVYDKNYDPSAELAILGRLSEKLHLMTITDLTQESLALHEMVASGGGQDPGEHIERMSMLLKKIKDFVQTQNPDMGPPMASRVLDSNGDSRPITIPDEFRCPISLELMKDPVIVSTGQTYERACIEKWIASGHHTCPTTQQKMSTSALTPNYVLRSLISQWCETNGMEPPKRSTQPNKPTPACSSSERANIDALLSKLCSPDTEEQRSAAAELRLLAKRNANNRICIAEAGAIPLLLSLLSSSDLRTQEHAVTALLNLSIHEDNKASIISSGAVPSIVHVLKNGSMEARENAAATLFSLSVIDEYKVTIGGMGAIPALVVLLGEGSQRGKKDAAAALFNLCIYQGNKGRAIRAGLVPLIMGLVTNPTGALMDEAMAILSILSSHPEGKAAIGAAEPVPVLVEMIGSGTPRNRENAAAVMLHLCSGEHHLVHLARAQECGIMVPLRELALNGTDRGKRKAVQLLERMSRFLVQQQEEQESQSQASAQVPPQATPEQVPENDIPEQLDSPASQYPMVV*