Information report for BPH26;BPH18
Gene Details
|
|
Functional Descriptions
- Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52.
- The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (brown planthopper resistance 26) and BPH25.
- Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein.
- BPH26 mediated sucking inhibition in the phloem sieve element.
- The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf sheath, especially in phloem cells where the BPH attacks.
- Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest.
- However, these two genes have remarkable sequence differences and the independent NILs showed differential BPH resistance with different expression patterns of plant defense-related genes, indicating that BPH18 and BPH26 are functionally different alleles.
- Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein.
- The BPH18 proteins were widely localized to the endo-membranes in a cell, including the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and prevacuolar compartments, suggesting that BPH18 may recognize the BPH invasion at endo-membranes in phloem cells.
- BPH18 has two NBS domains, unlike the typical NBS-LRR proteins.
Functional Keywords
- resistance , map-based-cloning , brown-planthopper , phloem , leaf , vascular-bundle , insect , R-protein
Literature and News
- Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52 . DOI: 10.1038/srep05872 ; PMID: 25076167
- Erratum: Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest . DOI: 10.1038/srep36688 ; PMID: 27834403
Gene Resources
- PANTHER: PTHR23155, PTHR23155:SF1114
- SUPFAM: SSF52058, SSF52540
- Gene3D: 1.10.10.10, 1.10.8.430, 3.80.10.10
Homologs
- Saccharum spontaneum: Sspon.02G0022550-1A
- Setaria italica: Seita.6G229200
Sequences
cDNA Sequence
- >LOC_Os12g37280.1
ATGTGGGCAGGCAGCACAAAAGGGGTGCGGGTAGCTGTGGCAGCACCCCCTTCTTCCCCATCTACAGCCAAGGCATTTCAGCAACAGCAGCTCCACACACATGGTCAACACCGAGGTTCCTCTGCTCCCTCGCCACCCCATCGAACGCCCGAACCTGTTGCTCCCGTATGGCACCTACGACCCCATGGCTCAACGCGGGCGACGGTGAACTCATGGCAGAGGTGGGATGGGTGGCTTCAAACTCACAATGGAAGTGGTGAGATCAACGGCGAGATCCACAACCTCAACACCGCTTGCATCCACTATCTCTGTCACGGTGGGGGTGGGGGTAAATCGAACGGACGCATAGGGGTATTTAAGGAGTCAGTAAATTTGGATCAAGAAGATCTTGAGTTGATTGAAGAAGCAAAACTGATTCTAAAGAAGTGCAAAGGACTTCCCCTCGCAATTGTCACCATAGGTGGTTTCTTGGCAAGCCGGCCCAAAACTGCTTTGGAGTGGAGAAAATTGAATGAGCATATTAGTGCAGAGTTGGAGACAAACCTAGAGCTTGAGGCCATAAGAGCTGTCCTTAATATAAGCTACGATGGATTACCCTATCACCTCAAGTCTTGCTTCTTGTACCTGTCCATCTTTCCTCAAGATGACAAGATTAGCCGGAAACGTTTGGTTCACGATATCATGCGCGAGATAGCCATCTCGAAGTCAAAGGAGGAAAATCTTGTTCTTAGACTCGAAGGGGGTCGTAGGCTACACAATCATGACACAGTTCGGCATCTTTCCATTACAAACAGCAGCGAGGACTGGGAGACAGATGTCGGTGAATTGAAGACAACAGTAGACATGTCCCGAATAAGATCATTAACAGTGTTTGGGGAGTGGAGGCCATTTTTCATTTCTGACAAGATGAGGTTGTTGCGTGTGCTCGACTTGGAAGACACAAAAGATGTACGTAATCACCATATTAAGCAAATAGGGGAGCTTCTTCACCTTAGATACCTTTCTCTAAGAGGATGTATGCGCATTGCTTACCTGCCTGATTCTTTGGGTAACCTAAGGCAACTGGAGACACTAGATGTCAGAGATACGTTCATACTCAGGTTGCCAAAGACCATCACTAATCTTCGCAAGCTAAAGTATCTCCGTGCAATTGTAGACAAAATCACCTATGAAGGAATAGCAGAGGAACTACCAGAGAAAAACAGCAAAAAGTTTTTTTCTGTCCTTGCCGCGCTCAGCCGCCTAGAATCATTGTCGCTGCTCTCGAAGGGAAAGCCAGGTCTCTGCGGCTGTCTAGATGCTCAAGAAAAGTTTTCACCACCTAAGGATGTCAAGAGCCTGAAGCTGCAAGGCAACCTGGTTGAGTTGCCAAAATGGATCAAGCAGCTCAACAATCTCGTGAAGCTGAAGCTATCAGAAACAATGCTAAAGGATCATGATGCTGCTATACAAGTCCTTGGTATGCTACCAAACCTGACCATCCTATGCCTGTCGCGTGAGTCGTTTCACTCGCTTGAGGGTGAAGAACTCAATTTCTCGGAGGGATCTTTCAAAAGCCTGGTGGTTCTCGAGCTCAACTTCAGTGGGAGCAAATGTGTCAAGTTTGAACAAGGAGCGTTCCTCAATCTTGAGCTACTGCTGCTTTCAGTTTACTATGAAGAAGTTGAAACTAAGTTCTCTGGGCTAGAATTTCTCCAAAGCATCAAGGAAGTCCAGATCGATGGTTATTGCCCAAATAGGAAAGGATTGAAGAAAGACTTGCTGCCGCCTGGAGCGGAATCATTGTCGCTGCTCTCGAAGGGGGAGCCAGGTCTCTGGGGTTGTCTTGATGCTGATGAAGAGTTTTCACCACCTATGAATCTCAAGAGCCTGAAGCTGCAAGGCAACCTGGTTGAGTTGCCAAAATGGATCAGGCAGCTCAACAATCTCGTGAAGCTGAAGCTATCAGAAACAATGCTAAAGGATCATAATGCTACTATACAAGTCCTTGGTGAGCTAAGAAACCTGACCATCCTATGCCTGTCGCGCGAGCCGTTTCACTCGCTTGAGGGTGGCGAACTCAATTTCTCGGAGGGATCTTTCATAAGCCTGGTGGTTCTCGAGCTCCACTTCGGTGGGAGCAAATGTGTCAAGTTTGAACAAGGAGCGTTCCTCAATCTTGAGCTACTGCTGCTTTCAGTTTACTATGAAGAAGTTGAAACTAAGTTCTCTGGGCTAGAATTTCTCCCAGGAATCAAGGAAGTCCGGCTCCATGGTGAATTTTATGCCAGAAACGAGCAATCCGCACCAAGATTGAAAGAGGACTTGCTGGCACAGCTTTCTGAGAATCCAAAGAAACCAATCCTGAAGACTAGCGGGTGTTTTTAG
CDS Sequence
- >LOC_Os12g37280.1
ATGTGGGCAGGCAGCACAAAAGGGGTGCGGGTAGCTGTGGCAGCACCCCCTTCTTCCCCATCTACAGCCAAGGCATTTCAGCAACAGCAGCTCCACACACATGGTCAACACCGAGGTTCCTCTGCTCCCTCGCCACCCCATCGAACGCCCGAACCTGTTGCTCCCGTATGGCACCTACGACCCCATGGCTCAACGCGGGCGACGGTGAACTCATGGCAGAGGTGGGATGGGTGGCTTCAAACTCACAATGGAAGTGGTGAGATCAACGGCGAGATCCACAACCTCAACACCGCTTGCATCCACTATCTCTGTCACGGTGGGGGTGGGGGTAAATCGAACGGACGCATAGGGGTATTTAAGGAGTCAGTAAATTTGGATCAAGAAGATCTTGAGTTGATTGAAGAAGCAAAACTGATTCTAAAGAAGTGCAAAGGACTTCCCCTCGCAATTGTCACCATAGGTGGTTTCTTGGCAAGCCGGCCCAAAACTGCTTTGGAGTGGAGAAAATTGAATGAGCATATTAGTGCAGAGTTGGAGACAAACCTAGAGCTTGAGGCCATAAGAGCTGTCCTTAATATAAGCTACGATGGATTACCCTATCACCTCAAGTCTTGCTTCTTGTACCTGTCCATCTTTCCTCAAGATGACAAGATTAGCCGGAAACGTTTGGTTCACGATATCATGCGCGAGATAGCCATCTCGAAGTCAAAGGAGGAAAATCTTGTTCTTAGACTCGAAGGGGGTCGTAGGCTACACAATCATGACACAGTTCGGCATCTTTCCATTACAAACAGCAGCGAGGACTGGGAGACAGATGTCGGTGAATTGAAGACAACAGTAGACATGTCCCGAATAAGATCATTAACAGTGTTTGGGGAGTGGAGGCCATTTTTCATTTCTGACAAGATGAGGTTGTTGCGTGTGCTCGACTTGGAAGACACAAAAGATGTACGTAATCACCATATTAAGCAAATAGGGGAGCTTCTTCACCTTAGATACCTTTCTCTAAGAGGATGTATGCGCATTGCTTACCTGCCTGATTCTTTGGGTAACCTAAGGCAACTGGAGACACTAGATGTCAGAGATACGTTCATACTCAGGTTGCCAAAGACCATCACTAATCTTCGCAAGCTAAAGTATCTCCGTGCAATTGTAGACAAAATCACCTATGAAGGAATAGCAGAGGAACTACCAGAGAAAAACAGCAAAAAGTTTTTTTCTGTCCTTGCCGCGCTCAGCCGCCTAGAATCATTGTCGCTGCTCTCGAAGGGAAAGCCAGGTCTCTGCGGCTGTCTAGATGCTCAAGAAAAGTTTTCACCACCTAAGGATGTCAAGAGCCTGAAGCTGCAAGGCAACCTGGTTGAGTTGCCAAAATGGATCAAGCAGCTCAACAATCTCGTGAAGCTGAAGCTATCAGAAACAATGCTAAAGGATCATGATGCTGCTATACAAGTCCTTGGTATGCTACCAAACCTGACCATCCTATGCCTGTCGCGTGAGTCGTTTCACTCGCTTGAGGGTGAAGAACTCAATTTCTCGGAGGGATCTTTCAAAAGCCTGGTGGTTCTCGAGCTCAACTTCAGTGGGAGCAAATGTGTCAAGTTTGAACAAGGAGCGTTCCTCAATCTTGAGCTACTGCTGCTTTCAGTTTACTATGAAGAAGTTGAAACTAAGTTCTCTGGGCTAGAATTTCTCCAAAGCATCAAGGAAGTCCAGATCGATGGTTATTGCCCAAATAGGAAAGGATTGAAGAAAGACTTGCTGCCGCCTGGAGCGGAATCATTGTCGCTGCTCTCGAAGGGGGAGCCAGGTCTCTGGGGTTGTCTTGATGCTGATGAAGAGTTTTCACCACCTATGAATCTCAAGAGCCTGAAGCTGCAAGGCAACCTGGTTGAGTTGCCAAAATGGATCAGGCAGCTCAACAATCTCGTGAAGCTGAAGCTATCAGAAACAATGCTAAAGGATCATAATGCTACTATACAAGTCCTTGGTGAGCTAAGAAACCTGACCATCCTATGCCTGTCGCGCGAGCCGTTTCACTCGCTTGAGGGTGGCGAACTCAATTTCTCGGAGGGATCTTTCATAAGCCTGGTGGTTCTCGAGCTCCACTTCGGTGGGAGCAAATGTGTCAAGTTTGAACAAGGAGCGTTCCTCAATCTTGAGCTACTGCTGCTTTCAGTTTACTATGAAGAAGTTGAAACTAAGTTCTCTGGGCTAGAATTTCTCCCAGGAATCAAGGAAGTCCGGCTCCATGGTGAATTTTATGCCAGAAACGAGCAATCCGCACCAAGATTGAAAGAGGACTTGCTGGCACAGCTTTCTGAGAATCCAAAGAAACCAATCCTGAAGACTAGCGGGTGTTTTTAG
Protein Sequence
- >LOC_Os12g37280.1
MWAGSTKGVRVAVAAPPSSPSTAKAFQQQQLHTHGQHRGSSAPSPPHRTPEPVAPVWHLRPHGSTRATVNSWQRWDGWLQTHNGSGEINGEIHNLNTACIHYLCHGGGGGKSNGRIGVFKESVNLDQEDLELIEEAKLILKKCKGLPLAIVTIGGFLASRPKTALEWRKLNEHISAELETNLELEAIRAVLNISYDGLPYHLKSCFLYLSIFPQDDKISRKRLVHDIMREIAISKSKEENLVLRLEGGRRLHNHDTVRHLSITNSSEDWETDVGELKTTVDMSRIRSLTVFGEWRPFFISDKMRLLRVLDLEDTKDVRNHHIKQIGELLHLRYLSLRGCMRIAYLPDSLGNLRQLETLDVRDTFILRLPKTITNLRKLKYLRAIVDKITYEGIAEELPEKNSKKFFSVLAALSRLESLSLLSKGKPGLCGCLDAQEKFSPPKDVKSLKLQGNLVELPKWIKQLNNLVKLKLSETMLKDHDAAIQVLGMLPNLTILCLSRESFHSLEGEELNFSEGSFKSLVVLELNFSGSKCVKFEQGAFLNLELLLLSVYYEEVETKFSGLEFLQSIKEVQIDGYCPNRKGLKKDLLPPGAESLSLLSKGEPGLWGCLDADEEFSPPMNLKSLKLQGNLVELPKWIRQLNNLVKLKLSETMLKDHNATIQVLGELRNLTILCLSREPFHSLEGGELNFSEGSFISLVVLELHFGGSKCVKFEQGAFLNLELLLLSVYYEEVETKFSGLEFLPGIKEVRLHGEFYARNEQSAPRLKEDLLAQLSENPKKPILKTSGCF*