Gene Details:

Functional Descriptions:

  • Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol.
  • Three different programs for predicting subcellular targeting indicated that OsNDPK1 and NP922751 were non-organellar, OsNDPK2 plastidic, and OsNDPK3 mitochondrial.
  • The WSL12 protein localized to the chloroplast.
  • These results indicate that the OsNDPK2 encoded by WSL12 plays an important role in chloroplast development and chlorophyll biosynthesis by regulating the expression levels of related genes.
  • Map-based cloning revealed that the WSL12 locus encoded OsNDPK2, one of the three nucleoside diphosphate kinases (OsNDPKs).
  • The WSL12 mutant showed higher superoxide anion level and enhanced sensitivity to abscisic acid (ABA) and salinity.
  • In addition, WSL12 also affects the response to abiotic stress, such as ABA and salinity in rice, and is beneficial to molecular breeding of stress tolerance.
  • The transcription pattern of many genes involved in chlorophyll biosynthesis, ABA synthesis, light signaling pathway, reactive oxygen species-scavenging pathway and the other two OsNDPKs was altered in the WSL12 mutant.
  • We isolated a rice mutant named YSS2 (young seedling stripe2) with a striated seedling phenotype beginning from leaf 2 of delayed plant growth.
  • Real-time RT-PCR analysis demonstrated that YSS2 transcripts accumulated highly in L4 sections at the early leaf development stage.
  • Chlorotic YSS2 seedlings have decreased pigment contents and impaired chloroplast development.
  • Expression levels of genes associated with Chl biosynthesis and photosynthesis in YSS2 were mostly decreased, but genes involved in chloroplast biogenesis were up-regulated compared to the wild type.
  • Our overall data suggest that YSS2 has important roles in chloroplast biogenesis.
  • The YSS2 allele encodes a NDPK2 (nucleoside diphosphate kinase 2) protein showing high similarity to other types of NDPKs.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os12g36194.1
    CTCTGCTCCTGCTCATCATCTTCTTCTTCTCCGCTCTCCCTAATCCAAAAAAAGGCGGCTGCTTTGGTCGCTCGCTCGCTCGCGCGGACGTTGGAGGAGAAGCAGCTACCGGGATGGACGCCATGGCCGTGCTCGCGAGGACCTCCCGCCCCGCCCCGACCCTCCTCGCCGCGACCTCCCCCGCGGTGTCCCGGCGCCCGGCGGCCGTCTCCTTCGCCGCCGCCGCCTCCCCGGGGTCCCGCGGCCGCGTCGCGCTGAGCGCCGCGTGGGGCGGGAGGGCGGCCAGGGGGCGCGTCTCCGCCGCCGGCCGCATCGTCGCGTCGTCGGTTGAGCAATCCTATATTATGATCAAGCCTGATGGTGTTCAGCGAGGCCTGGTTGGCGAGATTATTTCCCGTTTCGAGAAGAAAGGCTTTGTGTTGAAGGGCTTAAAGCTTTTTCAGTGCCCCAAGGACTTGGCACAGGAGCACTACAAGGATTTGAAGGAAAAACCTTTCTTTCCCGGTCTGATCGAGTACATAACTTCTGGCCCTGTTGTTTGCATGGCTTGGGAGGGGAATGGTGTTGTTGCATCAGCTCGCAAGTTAATAGGAGCTACTAATCCCCTTCAAGCTGAACCAGGGACCATACGGGGCGATCTTGCAGTTCAAACAGGAAGGAATGTTGTCCATGGAAGTGACAGTCCTGATAACGGCAAGCGTGAAATCGCTCTATGGTTCAAAGAAGGCGAACTCTGCGAATGGGAATCTGTTCTGACACCATGGCTTGTAGAGTAATGCTCCCAGATCAGTGCAAGAACTGGATGCAGTGCTTGTTCTTGATTTGAGGGGTTATGTATGAGTTCAGATCAGCAGAGTAAAGTAGCTTATACCAAAGCTCGGCCTTCTCATGACTGTACACCCTGACATTCCCCTTTTTTGTAATACCCAACATTCCCTTTTTTGCGTTTGACCGATTACTCTCTGTTGTCAAATCTCCTGGCATGTCTGTAATTGGTTCAGAACAATACCAGCAAAGCTCCAACTTCCAAGTCATTATGCAGATGAATGCGTTCAAAATTATCTCAGGCAGTTGACCGATGACTGATTGTCACTGTTGTACTATAGCTCCCAGCAGAGGTGAACTCGTGCAGAGTTGCAGAGTTCATTTGGCTGATGAGCCATATCTTGATCAGCAGTTATGAAATTGTCACCGTATTGGTGTTCAACCCACAAATGAGGAGGCTAAGGGATACTCGTTTGATCTTTTTTGGTGCTTGCATTTCTTGAGATAATGTTTTACTGCTTGATATTTCATCCCAGAAGCTCTCATTTGTACCTCAGCCTCTCAACACCTGAAAGTCTGGACACTGAAGGCATTGATAATTTTCTTACTTCATAGCCGAAAATAAATAAAATCTCAAAATCAAGACTATAGGTGAAAAACCAGCTAAGTGCTTGTGTTTGAGATTAGCCATAAAGCATATTGGCTCAACATGCGAAATTAAATCTGGAGCGTTGGGCACACTCAGGTAATTGTGGGGACTTGGGCCGTGCCGGTCTAGCACGGCATGGCCCATCGTGCTTCGTGCCGGCCC
CDS Sequence
  • >LOC_Os12g36194.1
    ATGGACGCCATGGCCGTGCTCGCGAGGACCTCCCGCCCCGCCCCGACCCTCCTCGCCGCGACCTCCCCCGCGGTGTCCCGGCGCCCGGCGGCCGTCTCCTTCGCCGCCGCCGCCTCCCCGGGGTCCCGCGGCCGCGTCGCGCTGAGCGCCGCGTGGGGCGGGAGGGCGGCCAGGGGGCGCGTCTCCGCCGCCGGCCGCATCGTCGCGTCGTCGGTTGAGCAATCCTATATTATGATCAAGCCTGATGGTGTTCAGCGAGGCCTGGTTGGCGAGATTATTTCCCGTTTCGAGAAGAAAGGCTTTGTGTTGAAGGGCTTAAAGCTTTTTCAGTGCCCCAAGGACTTGGCACAGGAGCACTACAAGGATTTGAAGGAAAAACCTTTCTTTCCCGGTCTGATCGAGTACATAACTTCTGGCCCTGTTGTTTGCATGGCTTGGGAGGGGAATGGTGTTGTTGCATCAGCTCGCAAGTTAATAGGAGCTACTAATCCCCTTCAAGCTGAACCAGGGACCATACGGGGCGATCTTGCAGTTCAAACAGGAAGGAATGTTGTCCATGGAAGTGACAGTCCTGATAACGGCAAGCGTGAAATCGCTCTATGGTTCAAAGAAGGCGAACTCTGCGAATGGGAATCTGTTCTGACACCATGGCTTGTAGAGTAA
Protein Sequence
  • >LOC_Os12g36194.1
    MDAMAVLARTSRPAPTLLAATSPAVSRRPAAVSFAAAASPGSRGRVALSAAWGGRAARGRVSAAGRIVASSVEQSYIMIKPDGVQRGLVGEIISRFEKKGFVLKGLKLFQCPKDLAQEHYKDLKEKPFFPGLIEYITSGPVVCMAWEGNGVVASARKLIGATNPLQAEPGTIRGDLAVQTGRNVVHGSDSPDNGKREIALWFKEGELCEWESVLTPWLVE*