Information report for OsNDPK2;WSL12
Gene Details
|
|
Functional Descriptions
- Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol.
- Three different programs for predicting subcellular targeting indicated that OsNDPK1 and NP922751 were non-organellar, OsNDPK2 plastidic, and OsNDPK3 mitochondrial.
- The WSL12 protein localized to the chloroplast.
- These results indicate that the OsNDPK2 encoded by WSL12 plays an important role in chloroplast development and chlorophyll biosynthesis by regulating the expression levels of related genes.
- Map-based cloning revealed that the WSL12 locus encoded OsNDPK2, one of the three nucleoside diphosphate kinases (OsNDPKs).
- The WSL12 mutant showed higher superoxide anion level and enhanced sensitivity to abscisic acid (ABA) and salinity.
- In addition, WSL12 also affects the response to abiotic stress, such as ABA and salinity in rice, and is beneficial to molecular breeding of stress tolerance.
- The transcription pattern of many genes involved in chlorophyll biosynthesis, ABA synthesis, light signaling pathway, reactive oxygen species-scavenging pathway and the other two OsNDPKs was altered in the WSL12 mutant.
- We isolated a rice mutant named YSS2 (young seedling stripe2) with a striated seedling phenotype beginning from leaf 2 of delayed plant growth.
- Real-time RT-PCR analysis demonstrated that YSS2 transcripts accumulated highly in L4 sections at the early leaf development stage.
- Chlorotic YSS2 seedlings have decreased pigment contents and impaired chloroplast development.
- Expression levels of genes associated with Chl biosynthesis and photosynthesis in YSS2 were mostly decreased, but genes involved in chloroplast biogenesis were up-regulated compared to the wild type.
- Our overall data suggest that YSS2 has important roles in chloroplast biogenesis.
- The YSS2 allele encodes a NDPK2 (nucleoside diphosphate kinase 2) protein showing high similarity to other types of NDPKs.
Functional Keywords
- mitochondria , chloroplast , development , map-based-cloning , salinity , tolerance , abiotic-stress , ABA , stress , breeding , biotic-stress , abscisic-acid , stress-tolerance , reactive-oxygen-species , chloroplast-development , leaf , seedlings , growth , leaf-development , seedling , photosynthesis , Chl-biosynthesis , Kinase , plant-growth
Literature and News
- Physiological and biochemical characterization of three nucleoside diphosphate kinase isozymes from rice (Oryza sativa L.) . DOI: 10.1271/bbb.110285 ; PMID: 21897044
- Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 (OsNDPK1) in rice plants upon infection with bacterial pathogens . DOI: ; PMID: 15650338
- White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativa L.) . DOI: 10.1007/s11032-016-0479-6 ; PMID: 27212889
- A Young Seedling Stripe2 phenotype in rice is caused by mutation of a chloroplast-localized nucleoside diphosphate kinase 2 required for chloroplast biogenesis . DOI: 10.1590/1678-4685-GMB-2016-0267 ; PMID: 28863212
Gene Resources
- UniProt: Q0IMS5
- EMBL: AK070054, AP014968, DP000011
- AlphaFoldDB: Q0IMS5
- EnsemblPlants: Os12t0548300-01
- Gramene: Os12t0548300-01
- KEGG: dosa:Os12g0548300, osa:4352460
- Orthologous matrix: LLNWECC
- InterPro: IPR001564, IPR023005, IPR034907
Sequences
cDNA Sequence
- >LOC_Os12g36194.1
CTCTGCTCCTGCTCATCATCTTCTTCTTCTCCGCTCTCCCTAATCCAAAAAAAGGCGGCTGCTTTGGTCGCTCGCTCGCTCGCGCGGACGTTGGAGGAGAAGCAGCTACCGGGATGGACGCCATGGCCGTGCTCGCGAGGACCTCCCGCCCCGCCCCGACCCTCCTCGCCGCGACCTCCCCCGCGGTGTCCCGGCGCCCGGCGGCCGTCTCCTTCGCCGCCGCCGCCTCCCCGGGGTCCCGCGGCCGCGTCGCGCTGAGCGCCGCGTGGGGCGGGAGGGCGGCCAGGGGGCGCGTCTCCGCCGCCGGCCGCATCGTCGCGTCGTCGGTTGAGCAATCCTATATTATGATCAAGCCTGATGGTGTTCAGCGAGGCCTGGTTGGCGAGATTATTTCCCGTTTCGAGAAGAAAGGCTTTGTGTTGAAGGGCTTAAAGCTTTTTCAGTGCCCCAAGGACTTGGCACAGGAGCACTACAAGGATTTGAAGGAAAAACCTTTCTTTCCCGGTCTGATCGAGTACATAACTTCTGGCCCTGTTGTTTGCATGGCTTGGGAGGGGAATGGTGTTGTTGCATCAGCTCGCAAGTTAATAGGAGCTACTAATCCCCTTCAAGCTGAACCAGGGACCATACGGGGCGATCTTGCAGTTCAAACAGGAAGGAATGTTGTCCATGGAAGTGACAGTCCTGATAACGGCAAGCGTGAAATCGCTCTATGGTTCAAAGAAGGCGAACTCTGCGAATGGGAATCTGTTCTGACACCATGGCTTGTAGAGTAATGCTCCCAGATCAGTGCAAGAACTGGATGCAGTGCTTGTTCTTGATTTGAGGGGTTATGTATGAGTTCAGATCAGCAGAGTAAAGTAGCTTATACCAAAGCTCGGCCTTCTCATGACTGTACACCCTGACATTCCCCTTTTTTGTAATACCCAACATTCCCTTTTTTGCGTTTGACCGATTACTCTCTGTTGTCAAATCTCCTGGCATGTCTGTAATTGGTTCAGAACAATACCAGCAAAGCTCCAACTTCCAAGTCATTATGCAGATGAATGCGTTCAAAATTATCTCAGGCAGTTGACCGATGACTGATTGTCACTGTTGTACTATAGCTCCCAGCAGAGGTGAACTCGTGCAGAGTTGCAGAGTTCATTTGGCTGATGAGCCATATCTTGATCAGCAGTTATGAAATTGTCACCGTATTGGTGTTCAACCCACAAATGAGGAGGCTAAGGGATACTCGTTTGATCTTTTTTGGTGCTTGCATTTCTTGAGATAATGTTTTACTGCTTGATATTTCATCCCAGAAGCTCTCATTTGTACCTCAGCCTCTCAACACCTGAAAGTCTGGACACTGAAGGCATTGATAATTTTCTTACTTCATAGCCGAAAATAAATAAAATCTCAAAATCAAGACTATAGGTGAAAAACCAGCTAAGTGCTTGTGTTTGAGATTAGCCATAAAGCATATTGGCTCAACATGCGAAATTAAATCTGGAGCGTTGGGCACACTCAGGTAATTGTGGGGACTTGGGCCGTGCCGGTCTAGCACGGCATGGCCCATCGTGCTTCGTGCCGGCCC
CDS Sequence
- >LOC_Os12g36194.1
ATGGACGCCATGGCCGTGCTCGCGAGGACCTCCCGCCCCGCCCCGACCCTCCTCGCCGCGACCTCCCCCGCGGTGTCCCGGCGCCCGGCGGCCGTCTCCTTCGCCGCCGCCGCCTCCCCGGGGTCCCGCGGCCGCGTCGCGCTGAGCGCCGCGTGGGGCGGGAGGGCGGCCAGGGGGCGCGTCTCCGCCGCCGGCCGCATCGTCGCGTCGTCGGTTGAGCAATCCTATATTATGATCAAGCCTGATGGTGTTCAGCGAGGCCTGGTTGGCGAGATTATTTCCCGTTTCGAGAAGAAAGGCTTTGTGTTGAAGGGCTTAAAGCTTTTTCAGTGCCCCAAGGACTTGGCACAGGAGCACTACAAGGATTTGAAGGAAAAACCTTTCTTTCCCGGTCTGATCGAGTACATAACTTCTGGCCCTGTTGTTTGCATGGCTTGGGAGGGGAATGGTGTTGTTGCATCAGCTCGCAAGTTAATAGGAGCTACTAATCCCCTTCAAGCTGAACCAGGGACCATACGGGGCGATCTTGCAGTTCAAACAGGAAGGAATGTTGTCCATGGAAGTGACAGTCCTGATAACGGCAAGCGTGAAATCGCTCTATGGTTCAAAGAAGGCGAACTCTGCGAATGGGAATCTGTTCTGACACCATGGCTTGTAGAGTAA
Protein Sequence
- >LOC_Os12g36194.1
MDAMAVLARTSRPAPTLLAATSPAVSRRPAAVSFAAAASPGSRGRVALSAAWGGRAARGRVSAAGRIVASSVEQSYIMIKPDGVQRGLVGEIISRFEKKGFVLKGLKLFQCPKDLAQEHYKDLKEKPFFPGLIEYITSGPVVCMAWEGNGVVASARKLIGATNPLQAEPGTIRGDLAVQTGRNVVHGSDSPDNGKREIALWFKEGELCEWESVLTPWLVE*