Gene Details:

Functional Descriptions:

  • Mutation of the rice TCM12 gene encoding 2,3-bisphosphoglycerate-independent phosphoglycerate mutase affects chlorophyll synthesis, photosynthesis and chloroplast development at seedling stage at low temperatures.
  • The inheritance behaviour and function of TCM12 were then analysed thorough map-based cloning, transgenic complementation and subcellular localisation.
  • In addition, TCM12 was constitutively expressed, and its disruption resulted in down-regulation of some genes associated with chlorophyll biosynthesis and photosynthesis at low temperatures (20 °C).
  • The thermo-sensitive chlorosis phenotype was caused by a single nucleotide mutation (T→C) on the fifth exon of TCM12 (LOC_Os12g35040) encoding iPGAM, localised to both nucleus and membranes.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os12g35040.1
    GAAGAGAGTGAAAAAAAAAAAAAGCAAAAGCGGCGACGCCACACTAGCCAGCGGCGGAGGCGGTGGAGAGGAGAGGAGAGGGGAGAGGGGGGAAGCAAAGCGCGAGCGGCGATGGCGGCGAGGAGGAGCAGGGTGGCGTTCGTGCTGGTGGACGGGATCGGGGACGTGAGCATCCCGTCGCTCCGGGGGCGGACGCCGCTGGAGGCGGCGGCGGCGCCGGGGCTGGACGCGGTGGCGGCGGCCGGGGTGGCGGGGGCTCATGGACCCCGTCGAGCCGGGGCTCGCCTGCGGGAGCGACACGGCGCACCTCTCCCTCCTCGGGTACGACCCCCGCGTGTACTACCGCGGCCGCGGCGCGTTCGAGTCCATGGGCGCCGGCCTCGCCATGGCCCCCGGCGACATCGCCTTCAAGTCAAACTTTGCTACGTTGGATGAAAGCACGGGGATCATTGTCAGCAGAAGGGCGGATCGACACTTTGAGGAGGAAGGCCCGATACTCTGTGCTGCATTGGATGGGTTGAAGCTCCCATCGTTTCCTGAATATGAAGTGAGAGTCAGATATGCTACTGAACACAGGTGTGGTGTGGTTGTGAAAGGACCGAGGCTGAGTGGGAACATTTCTGGAACTGATCCATTGAAGGACAACCGCTTGCATCTAAAGGCTGAACCTTTGGATGATTCAGAAGAAGCTAAAAACACGGCAGCTGTGGTCAATGAGCTTTCTAAGGAGATTACACGTATACTGGTGTCCCATCCTATCAATGCACAGCGCTCTGCCGAGGGGAAGAACATTGCAAATGTTGTGTTATTGCGAGGCTGTGGTATCCGGATTGAGGTTCCTGCCTTCGAAACCAAGCATGGACTTGCACCATGCATGGTGGCTCCTACAAAGATCATAGCTGGGCTGGGGCTCTCACTGGGCATTGATATCCTTGAAGCACCTGGAGCAACTGGAGACTATCGAACTCTCTTGACTTCCAAAGCTAAAGCTATAGCCAAGGCACTGTCTGCTCCTCTGGATACGCCACCTCGTGTTTTTGTGCCTGGAGAGGATGAATATAAATCTGGAAAGGAAAATGGATATGACTTTGGGTTCTTGCACATAAAGGCCATCGATGATGCCGGTCATGATAAGGCTGTCAAGCTTAAGGTACTAGGCCTAGAGGCTGTTGATCGGGCTATCGTTCAGCTTGCAAGGCTCCTGTGGGAAGCTGAAAAGTCTGGGAAATACCAGTATTTCCTTTGTGTCACCGGAGACCACTCTACTCCAGTTGAGTACGGTGACCACAGTTTTGAACCCGTTCCTTTTGTGTTGTGCCGACTTAGAGATTTCGTAGGAGCTATGGGGGAGGACAATGTTATCAACTTCCCCCTGGACGATTTCCTGCTTCCTTCAGTTAAATCTGGAGAAGATGTGGCAGAAAACATAGATGTAGCTGATCGCAAGTCTGATCAGCGCAAAGCTTTCAGTGGCGATTCTGTCTTTGAATTCAACGAGATTGCCGCTGCAAGGGGCTGCCTTGGACGTTTCCCTGGAAGTGAGATGATGGGCATCATCAAGAAGTTCATCAAAGCGAAGAACGATTAGTGCCAGTTCAACATACTGACGTTTGTGTAATCTGTTGACCTTGAGGCTTCCATGTCTGTAATAATTGCAAACAAGGATGTATTCAGAGAACCAGCTCAGTCTGAGATGAATAAGGCTTCTCATGGATCTTGAATCTTGCACTGAAAAGCATAAATAATCTGACCTGCTGTGTACATTTACCAAG
CDS Sequence
  • >LOC_Os12g35040.1
    ATGGACCCCGTCGAGCCGGGGCTCGCCTGCGGGAGCGACACGGCGCACCTCTCCCTCCTCGGGTACGACCCCCGCGTGTACTACCGCGGCCGCGGCGCGTTCGAGTCCATGGGCGCCGGCCTCGCCATGGCCCCCGGCGACATCGCCTTCAAGTCAAACTTTGCTACGTTGGATGAAAGCACGGGGATCATTGTCAGCAGAAGGGCGGATCGACACTTTGAGGAGGAAGGCCCGATACTCTGTGCTGCATTGGATGGGTTGAAGCTCCCATCGTTTCCTGAATATGAAGTGAGAGTCAGATATGCTACTGAACACAGGTGTGGTGTGGTTGTGAAAGGACCGAGGCTGAGTGGGAACATTTCTGGAACTGATCCATTGAAGGACAACCGCTTGCATCTAAAGGCTGAACCTTTGGATGATTCAGAAGAAGCTAAAAACACGGCAGCTGTGGTCAATGAGCTTTCTAAGGAGATTACACGTATACTGGTGTCCCATCCTATCAATGCACAGCGCTCTGCCGAGGGGAAGAACATTGCAAATGTTGTGTTATTGCGAGGCTGTGGTATCCGGATTGAGGTTCCTGCCTTCGAAACCAAGCATGGACTTGCACCATGCATGGTGGCTCCTACAAAGATCATAGCTGGGCTGGGGCTCTCACTGGGCATTGATATCCTTGAAGCACCTGGAGCAACTGGAGACTATCGAACTCTCTTGACTTCCAAAGCTAAAGCTATAGCCAAGGCACTGTCTGCTCCTCTGGATACGCCACCTCGTGTTTTTGTGCCTGGAGAGGATGAATATAAATCTGGAAAGGAAAATGGATATGACTTTGGGTTCTTGCACATAAAGGCCATCGATGATGCCGGTCATGATAAGGCTGTCAAGCTTAAGGTACTAGGCCTAGAGGCTGTTGATCGGGCTATCGTTCAGCTTGCAAGGCTCCTGTGGGAAGCTGAAAAGTCTGGGAAATACCAGTATTTCCTTTGTGTCACCGGAGACCACTCTACTCCAGTTGAGTACGGTGACCACAGTTTTGAACCCGTTCCTTTTGTGTTGTGCCGACTTAGAGATTTCGTAGGAGCTATGGGGGAGGACAATGTTATCAACTTCCCCCTGGACGATTTCCTGCTTCCTTCAGTTAAATCTGGAGAAGATGTGGCAGAAAACATAGATGTAGCTGATCGCAAGTCTGATCAGCGCAAAGCTTTCAGTGGCGATTCTGTCTTTGAATTCAACGAGATTGCCGCTGCAAGGGGCTGCCTTGGACGTTTCCCTGGAAGTGAGATGATGGGCATCATCAAGAAGTTCATCAAAGCGAAGAACGATTAG
Protein Sequence
  • >LOC_Os12g35040.1
    MDPVEPGLACGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMAPGDIAFKSNFATLDESTGIIVSRRADRHFEEEGPILCAALDGLKLPSFPEYEVRVRYATEHRCGVVVKGPRLSGNISGTDPLKDNRLHLKAEPLDDSEEAKNTAAVVNELSKEITRILVSHPINAQRSAEGKNIANVVLLRGCGIRIEVPAFETKHGLAPCMVAPTKIIAGLGLSLGIDILEAPGATGDYRTLLTSKAKAIAKALSAPLDTPPRVFVPGEDEYKSGKENGYDFGFLHIKAIDDAGHDKAVKLKVLGLEAVDRAIVQLARLLWEAEKSGKYQYFLCVTGDHSTPVEYGDHSFEPVPFVLCRLRDFVGAMGEDNVINFPLDDFLLPSVKSGEDVAENIDVADRKSDQRKAFSGDSVFEFNEIAAARGCLGRFPGSEMMGIIKKFIKAKND*