Gene Details:
- MSU gene ID: LOC_Os12g29220
- RAPdb gene ID: Os12g0476200
- Gene Symbol: Xa25 OsSWEET13
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Transformation of the dominant Xa25 into a resistant rice line carrying the recessive Xa25 abolished its resistance to PXO339.
- A new dominant gene for bacterial blight resistance in rice, Xa25(t), was identified from Minghui 63, a restorer line for a number of rice hybrids that are widely cultivated in China.
- The Xa25, localized in the centromeric region of chromosome 12, mediates race-specific resistance to Xoo strain PXO339 at both seedling and adult stages by inhibiting Xoo growth.
- Here we report the characterization of another recessive resistance gene, Xa25, for Xoo resistance.
- The expression of dominant Xa25 but not recessive Xa25 was rapidly induced by PXO339 but not other Xoo strain infections.
- Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae.
- In this study, we identified candidate resistance alleles by mining a rice diversity panel for mutations in the promoter of OsSWEET13 and OsSWEET14, which are direct targets of three major TALEs PthXo2, PthXo3 and AvrXa7.
- Transactivation analyses showed that an abscisic acid (ABA)-responsive transcription factor OsbZIP72 directly binds to the promoters of OsSWEET13 and OsSWEET15 and activates their expression.
- Taken together, the results showed that the higher expressions of OsSWEET13 and OsSWEET15 genes, induced by binding of an ABA-responsive transcription factor OsbZIP72 to the promoters, potentially modulate sucrose transport and distribution in response to the abiotic stresses.
- Sucrose transport in response to drought and salt stress involves ABA-mediated induction of OsSWEET13 and OsSWEET15 in rice.
- Expression analyses of SWEET genes and histochemical analysis of
-glucuronidase-reporter transgenic plants suggested that OsSWEET13 and OsSWEET15 are major SWEET transporters regulating the sucrose transport and levels in response to the abiotic stresses.
Function-related keywords:
- resistant , blight , growth , bacterial-blight , seedling , xoo , resistance , transcription-factor , drought , salt , abiotic-stress , salt-stress , stress , biotic-stress , abscisic-acid , sucrose
Literature:
- New gene for bacterial blight resistance in rice located on chromosome 12 identified from minghui 63, an elite restorer line . DOI: 10.1094/PHYTO.2002.92.7.750 ; PMID: 18943271
- A paralog of the MtN3/saliva family recessively confers race-specific resistance to Xanthomonas oryzae in rice . DOI: 10.1111/j.1365-3040.2011.02391.x ; PMID: 21726237
- Natural variations in the promoter of OsSWEET13 and OsSWEET14 expand the range of resistance against Xanthomonas oryzae pv. oryzae . DOI: 10.1371/journal.pone.0203711 ; PMID: 30212546
- Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice . DOI: 10.1016/j.molp.2019.08.006 ; PMID: 31493565
- Broad-spectrum resistance to bacterial blight in rice using genome editing . DOI: 10.1038/s41587-019-0267-z ; PMID: 31659337
- Sucrose transport in response to drought and salt stress involves ABA-mediated induction of OsSWEET13 and OsSWEET15 in rice . DOI: 10.1111/ppl.13210 ; PMID: 32940908
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os12g29220.1
AAGAGTTTTCAGCCAACACATTGAACTCTTCTTCAGAGCTCTCCCTTCCCTCCACAAAGGGGGTCTAGGGTTAGAGTGTGTGTGTCTGTGACAAGTTCCAAGCTAGCAACAACAAGCTCAATTCCTTGCTTGTTTGCTTCCATATTACACTACATCTCTTCCCTTCAATTACCCCCCTTTTAGCACACAAAAATGGCTGGCCTGTCCCTGCAGCATCCCTGGGCTTTTGCCTTCGGCCTCCTTGGCAACCTCATATCCTTCACGACCTATCTGGCACCAATCCCGACGTTCTACCGGATCTACAAGAGCAAGTCGACGGAGGGGTTCCAGTCGGTGCCGTACGTGGTGGCGCTCTTCAGCGCCATGCTGTGGATCTTCTACGCGCTGATCAAGTCCAACGAGGCCCTCCTCATCACCATCAACGCCGCCGGTTGCGTCATCGAGACCATCTACATCGTCATGTACCTCGCCTACGCCCCCAAGAAGGCCAAGGTGTTCACGACGAAGATCCTGCTGCTGCTGAACGTGGGGGTGTTCGGGGTGATCCTGCTGCTGACGCTGCTGCTCTCCCATGGCGAGCAGCGCGTCGTCTCCCTCGGCTGGGTCTGCGTCGCCTTCTCCGTCAGCGTCTTTGTCGCGCCGCTCAGCATCATCAAGCGAGTGATCCAGTCGAGGAGCGTGGAGTACATGCCCTTCTCCCTCTCCCTCACGCTCACCCTCAGCGCCGTCGTCTGGTTCCTCTACGGCCTTCTCATCAAGGACAAATACGTCGCGCTTCCCAACATCCTGGGCTTCACATTCGGTGTGGTCCAGATGGGGCTCTACGTGTTCTACATGAACGCGACGCCGGTGGCCGGCGAGGGGAAAGAAGGGAAGGGGAAGCTGGCGGCGGCGGAGGAGCTCCCCGTCGTCGTCAACGTCGGCAAGCTCGCCGCCGCCACGCCCGACAGGAGCACCGGCGCCGTGCACGTGCACCCAGTCCCGAGGAGCTGCGCGGCGGAGGCGGCGGCGGCCGAGCCGGAGGTGCTCGTCGACATTCCGCCGCCGCCGCCGCCGCGCGCCGTCGAGGTGGCCGCCGTGTAGGGTCCCCGGCCGGTCAACGCGTGCTTGCATGGGCCATGCACGTGTGCAGTACCACGTGCCACGTACACTTTAATATGAACTCCAGGGCAAACTTGGAGGAGACAAGCTACCACCAATTAATTAACTTAATTATTATTACTCATCTTTCCCAAAATATAATTACTTTTAGCCTCTAAAATTTATCTCAAAATATAGCAACTTCTTCACCTATATTCTCTTATAAAACAATCGTAACCTTCCATTAATTAAATTTCTTACATATATATTTCTCCTCTTATCAATCAATCCCAACTGCCTTTTTTTTTCATTTTATTCCTGTAACTTTCGTAATATATCCGTATATCCTATCTAAAACTTATTATATTTTGGGATGGTGATGGTATTATATATCTTTCTTGCATGCCATGCAACTAGAGACGGGGGAAGCTATGTGTAATGTATGTGCATGTGTTTCTCTGGTGTTGGTTTCCGCTTCTGTTGTATTCATTTGTATCGGTCAATGGGTATCTATTATTCCTAATTAGTTCCTTGTACTTGTAATGTATGTCAAAATTAATAAAAATGTAATCGATAAATATCTCTTCTATCAATTGTGAAATACTCATGCACACATGATCTCTTATT
CDS Sequence
- >LOC_Os12g29220.1
ATGGCTGGCCTGTCCCTGCAGCATCCCTGGGCTTTTGCCTTCGGCCTCCTTGGCAACCTCATATCCTTCACGACCTATCTGGCACCAATCCCGACGTTCTACCGGATCTACAAGAGCAAGTCGACGGAGGGGTTCCAGTCGGTGCCGTACGTGGTGGCGCTCTTCAGCGCCATGCTGTGGATCTTCTACGCGCTGATCAAGTCCAACGAGGCCCTCCTCATCACCATCAACGCCGCCGGTTGCGTCATCGAGACCATCTACATCGTCATGTACCTCGCCTACGCCCCCAAGAAGGCCAAGGTGTTCACGACGAAGATCCTGCTGCTGCTGAACGTGGGGGTGTTCGGGGTGATCCTGCTGCTGACGCTGCTGCTCTCCCATGGCGAGCAGCGCGTCGTCTCCCTCGGCTGGGTCTGCGTCGCCTTCTCCGTCAGCGTCTTTGTCGCGCCGCTCAGCATCATCAAGCGAGTGATCCAGTCGAGGAGCGTGGAGTACATGCCCTTCTCCCTCTCCCTCACGCTCACCCTCAGCGCCGTCGTCTGGTTCCTCTACGGCCTTCTCATCAAGGACAAATACGTCGCGCTTCCCAACATCCTGGGCTTCACATTCGGTGTGGTCCAGATGGGGCTCTACGTGTTCTACATGAACGCGACGCCGGTGGCCGGCGAGGGGAAAGAAGGGAAGGGGAAGCTGGCGGCGGCGGAGGAGCTCCCCGTCGTCGTCAACGTCGGCAAGCTCGCCGCCGCCACGCCCGACAGGAGCACCGGCGCCGTGCACGTGCACCCAGTCCCGAGGAGCTGCGCGGCGGAGGCGGCGGCGGCCGAGCCGGAGGTGCTCGTCGACATTCCGCCGCCGCCGCCGCCGCGCGCCGTCGAGGTGGCCGCCGTGTAG
Protein Sequence
- >LOC_Os12g29220.1
MAGLSLQHPWAFAFGLLGNLISFTTYLAPIPTFYRIYKSKSTEGFQSVPYVVALFSAMLWIFYALIKSNEALLITINAAGCVIETIYIVMYLAYAPKKAKVFTTKILLLLNVGVFGVILLLTLLLSHGEQRVVSLGWVCVAFSVSVFVAPLSIIKRVIQSRSVEYMPFSLSLTLTLSAVVWFLYGLLIKDKYVALPNILGFTFGVVQMGLYVFYMNATPVAGEGKEGKGKLAAAEELPVVVNVGKLAAATPDRSTGAVHVHPVPRSCAAEAAAAEPEVLVDIPPPPPPRAVEVAAV*