Gene Details:

Functional Descriptions:

  • Here, we isolated and characterized the rice OsFIGNL1 (OsFidgetin-like 1) gene, encoding a conserved AAA-ATPase, and explored its function and importance in male meiosis and pollen formation.
  • The rice OsFIGNL1 mutant exhibited normal vegetative growth, but failed to produce seeds and displayed pollen abortion phenotype.
  • Phenotypic comparisons between the wild-type and OsFIGNL1 mutant demonstrated that OsFIGNL1 is required for anther development, and that the recessive mutation of this gene causes male sterility in rice.
  • Taken together, our results suggest that OsFIGNL1 plays an important role in regulation of male meiosis and anther development.
  • Complementation and CRISPR/Cas9 experiments demonstrated that wild-type OsFIGNL1 is responsible for the male sterility phenotype.
  • Male meiosis in the OsFIGNL1 mutant exhibited abnormal chromosome behavior, including chromosome bridges and multivalent chromosomes at diakinesis, lagging chromosomes, and chromosome fragments during meiosis.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os12g25720.1
    AGCAGAGACGCGCGGCGAAAGGAAAAAAAAAGAAAACAAAAAAGAAAAACACGCGAAATGGCGGAGCAGTCTCACGCCGGCGACGGCGGCGGCGGCGGCGCGGGCTCCGGGGAGCCGACGAACTGGAGGAAGGAGGCGGACGATAGGCTGCGGCGGCTCCACTCCCTCCAGTTCGGCGCGGACGTCGCGCTGAAGGGCAAGGACCCCGCGGGGGCGCAGGTGCTCGCGCTCCGCCTCCTCGGCTTCCTCGACTCGCAGGCCCTCCCCGGCGACGGCGGCGCGGCGGGCCACGAGGCCAGCTTCGTCGCGCCCATCCGCGCCGCGGCGTCATCCAGCGTCGCCGCCGCCATCCGCGCCCGCGCCGGGAGGTCAGACAGTGCTGTGTTTAAGCTTGCAGAAAAGGATGTTGGTTGTGTTTTTGCAAAGACAGGAGAAGTTAATATTGAAAAGATCAAGTGTTCAAAGTATTTTCAAGCACTTCTTCAGAAATCCAAAGGACATGCTGCTGAACAAATGAAGACCACTGATTGCCAAGAGTCCACCATTGAAGAAGGTCCGCATGTAGAGGAAAACTCAACTGATATGGAAAATGAGAAGCTTAGCATCAGGGCTTCAAAATTAGTAATGCAAAGAAAACTAACATCACTGCGTAGCCATAAGCCCCTGAAGGCAAATGTTGTACAAGATGGGAATATGTTCAAATCAGTGAGTAACATATCTAATGAGAGTGTTGCTGTCGAAAATGGAGTCAGAACAAATCATACTGATAACAAGTATACTGCTTATATGGATCTTGAAGATGATGACAGACCCCGTGGACTGTTGCAGAATGCAAAGCGAAAGCATGCAGGATTCAGAAGCCCAATCTGCGAACATGCAAATTCTCCATTAAGTAATGATGAAGCCGATGCCCCTGCCAATGAGTTTGTGACTGCCAGGACAAAGTTGGAGATGGATTCTGTGCAAAAGTATGGGCATAATGGTACTCAAGGTGCTTCTGTATCCCCACAATGTGATAACAACCCAAATAACCGGAATTATGGTGTAAGGCCAACCTGGAATTCTCGTCGTGGATTACGTGGTAATTTTGTTCCTCCTATCAGAAATAATGGAGGATCCACTTCCAATATGACCTCACGGGTCATTGGAAAAAATGATGATTCAATGGGAGATTCAACAAGAAAATGGTCTGTATTTCCATCTTGATCATATGATACCATTTGAATTTACTTTTATCTTCATGTTATTGTAACAATTTACATGACATTTACATAGCTATTTATCTCGTTGCTTTTCTTATGTTTAGCATGACTACATCTTTTCCTTTTTTTTCTAAGACTCAGATTTATTGGTACTAGAAGCAAAGTACATGAGATACATTTCTTTGACTTCATTGCCATAATGTGTTTTTTGTTTTGCAATTGAGATGACTAAGATTATCACTATCATATTCAGTATGATTGCCTTTCTTACATCTGTTTGGTGATTTGGACACTGGTTTGCATTTTTTGCTGGGTATCCTTGTTGTAGACGTTGCTTTAAAGTCAATGTAGTGTGTTAAAGAACTAGGTAAATTTCTGGATGGCAATATTACCTGTCACTTAACAATTTCAGAACAAGAGTATGAATTGATCAAGTGGATATAGCAGCATTAGTATGATAATAGATATTTGCTAAATGTG
CDS Sequence
  • >LOC_Os12g25720.1
    ATGGCGGAGCAGTCTCACGCCGGCGACGGCGGCGGCGGCGGCGCGGGCTCCGGGGAGCCGACGAACTGGAGGAAGGAGGCGGACGATAGGCTGCGGCGGCTCCACTCCCTCCAGTTCGGCGCGGACGTCGCGCTGAAGGGCAAGGACCCCGCGGGGGCGCAGGTGCTCGCGCTCCGCCTCCTCGGCTTCCTCGACTCGCAGGCCCTCCCCGGCGACGGCGGCGCGGCGGGCCACGAGGCCAGCTTCGTCGCGCCCATCCGCGCCGCGGCGTCATCCAGCGTCGCCGCCGCCATCCGCGCCCGCGCCGGGAGGTCAGACAGTGCTGTGTTTAAGCTTGCAGAAAAGGATGTTGGTTGTGTTTTTGCAAAGACAGGAGAAGTTAATATTGAAAAGATCAAGTGTTCAAAGTATTTTCAAGCACTTCTTCAGAAATCCAAAGGACATGCTGCTGAACAAATGAAGACCACTGATTGCCAAGAGTCCACCATTGAAGAAGGTCCGCATGTAGAGGAAAACTCAACTGATATGGAAAATGAGAAGCTTAGCATCAGGGCTTCAAAATTAGTAATGCAAAGAAAACTAACATCACTGCGTAGCCATAAGCCCCTGAAGGCAAATGTTGTACAAGATGGGAATATGTTCAAATCAGTGAGTAACATATCTAATGAGAGTGTTGCTGTCGAAAATGGAGTCAGAACAAATCATACTGATAACAAGTATACTGCTTATATGGATCTTGAAGATGATGACAGACCCCGTGGACTGTTGCAGAATGCAAAGCGAAAGCATGCAGGATTCAGAAGCCCAATCTGCGAACATGCAAATTCTCCATTAAGTAATGATGAAGCCGATGCCCCTGCCAATGAGTTTGTGACTGCCAGGACAAAGTTGGAGATGGATTCTGTGCAAAAGTATGGGCATAATGGTACTCAAGGTGCTTCTGTATCCCCACAATGTGATAACAACCCAAATAACCGGAATTATGGTGTAAGGCCAACCTGGAATTCTCGTCGTGGATTACGTGGTAATTTTGTTCCTCCTATCAGAAATAATGGAGGATCCACTTCCAATATGACCTCACGGGTCATTGGAAAAAATGATGATTCAATGGGAGATTCAACAAGAAAATGGTCTGTATTTCCATCTTGA
Protein Sequence
  • >LOC_Os12g25720.1
    MAEQSHAGDGGGGGAGSGEPTNWRKEADDRLRRLHSLQFGADVALKGKDPAGAQVLALRLLGFLDSQALPGDGGAAGHEASFVAPIRAAASSSVAAAIRARAGRSDSAVFKLAEKDVGCVFAKTGEVNIEKIKCSKYFQALLQKSKGHAAEQMKTTDCQESTIEEGPHVEENSTDMENEKLSIRASKLVMQRKLTSLRSHKPLKANVVQDGNMFKSVSNISNESVAVENGVRTNHTDNKYTAYMDLEDDDRPRGLLQNAKRKHAGFRSPICEHANSPLSNDEADAPANEFVTARTKLEMDSVQKYGHNGTQGASVSPQCDNNPNNRNYGVRPTWNSRRGLRGNFVPPIRNNGGSTSNMTSRVIGKNDDSMGDSTRKWSVFPS*