Information report for Adk-a;OsAK3
Gene Details
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Functional Descriptions
- In order to examine the enzyme activity of the protein encoded by the rice cDNA, Adk-a was cloned into an expression vector, pUC119, which was introduced into Escherichia coli strain CV2, a temperature-sensitive mutant of adenylate kinase.
- We found that the transformant carrying the rice Adk-a gene in the sense orientation recovered cell growth at non-permissive high temperature (42 degrees C) and expressed enzyme activities higher than the untransformed CV2 and the transformant possessing Adk-a cDNA in the antisense orientation.
- Two types of genes (Adk-a, and Adk-b) encoding for adenylate kinase (AK, EC 2.7.4.3.) were isolated from the cDNA library constructed from poly(A)+ RNA of rice (Oryza sativa L.).
- CONCLUSIONS: Our study reveals a novel BR signaling component OsAK3 in the regulation of grain length, and provides novel clues for uncovering the potential functions of OsAK3 in rice growth and development.
- In addition, RNA-Seq data indicate that OsAK3 is involved in a variety of biological processes that regulate BR signaling and grain development in rice.
- An Adenylate Kinase OsAK3 Involves Brassinosteroid Signaling and Grain Length in Rice (Oryza sativa L.).
- OsAK3 encodes an adenylate kinase, which regulates grain size by controlling cell expansion of rice spikelet glume.
- Among these mutants, mutant OsAK3 presents shorter grain length and dwarfing phenotype.
- Overexpression of OsAK3 resulted in longer grain length.
- Lamina inclination, coleoptile elongation and root inhibition experiments showed that the OsAK3 mutant was less sensitive to exogenous brassinolide (BL) treatment.
Functional Keywords
- temperature , growth , adenylate-kinase , development , Kinase , spikelet , grain , grain-length , grain-size , root , brassinosteroid , BR , Brassinosteroid , BR-signaling , lamina , kinase , Brassinosteroid-Signaling
Literature and News
- Molecular characterization of cDNA encoding for adenylate kinase of rice (Oryza sativa L.) . DOI: 10.1046/j.1365-313x.1992.t01-1-00999.x ; PMID: 1302636
- An Adenylate Kinase OsAK3 Involves Brassinosteroid Signaling and Grain Length in Rice (Oryza sativa L.) . DOI: 10.1186/s12284-021-00546-0 ; PMID: 34962599
Gene Resources
- UniProt: Q08479
- EMBL: AB041773, AK059754, AK069870
- AlphaFoldDB: Q08479
- EnsemblPlants: Os12t0236400-01
- Gramene: Os12t0236400-01
- KEGG: dosa:Os12g0236400
- Orthologous matrix: HYKVDAA
- InterPro: IPR000850, IPR006259, IPR007862
Homologs
- Sesamum indicum: SIN_1016656
- Solanum lycopersicum: Solyc09g007170.3
Sequences
cDNA Sequence
- >LOC_Os12g13380.1
GGTTGTCACCGTCTCACCAGAAACGAAACAAAAAGCCCCACCACCTAAACCTCCTCGATCCGACCGAGACTCCTCCATTTCAGCGGCGCACGCGGAGAGCACGCGACGCGAGTTCGTCGACGAACAAGGCTAGTGCAGTAGTTGTTGCTGCGGAGATGGCGGCGAACCTGGAGGACGTGCCGTCGATGGAGCTGATGACGGAGCTGCTCCGCCGCATGAAGTGCAGCTCCAAGCCCGACAAGCGCGTCATCCTCGTCGGTCCACCTGGCTGCGGAAAGGGAACACAGTCACCGCTGATTAAGGATGAATTTTGCTTGTGCCATTTAGCCACTGGTGATATGTTGAGGGCTGCAGTGGCTGCTAAAACTCCACTTGGGATTAAGGCTAAAGAAGCTATGGACAAGGGAGAGCTTGTTTCTGATGACTTGGTTGTTGGGATTATTGATGAAGCCATGAAGAAAACTTCATGCCAGAAAGGTTTTATCCTTGATGGTTTCCCTAGAACTGTTGTTCAAGCACAGAAGCTTGATGAAATGTTGGCCAAACAAGGTACTAAGATTGACAAGGTTCTAAATTTTGCAATTGATGATGCAATACTGGAAGAACGAATTACCGGTCGTTGGATCCACCCATCAAGTGGTAGATCTTATCATACAAAATTTGCTCCTCCTAAGACTCCTGGACTTGATGATGTTACTGGAGAACCCTTAATTCAAAGGAAAGATGACACAGCTGCAGTATTGAAGTCAAGGCTTGAAGCCTTCCACGTACAAACTAAGCCTGTGATTGACTACTACACCAAGAAGGGCATAGTCGCAAATCTGCATGCCGAAAAACCACCAAAGGAAGTGACTGTTGAGGTCCAGAAAGCCCTATCATAAACTGCCAGTTCTTCAGCCTATAAACATGACATGTGCCGAAAACCCCTTACACTGGGTTTTCTAGATGTTTAAACTGCTGCCAACACCATGTGGTTTTCATTTTGATTTGCTGTATGGCGCAATTGCTCCATTTTATCACAGTTTTCGCTTCACAGTCTGAAATTTGTCAAAATAATTTGTTGGAATGAGGAAAGAAGTTTTGTATACCAACACCGATATAATTACATCAAGTCAATCTTGACAGGGTATTCCTTCTGTTCATCTTAATTTTGGAATTGATGCCTTTGTGGTG
CDS Sequence
- >LOC_Os12g13380.1
ATGGCGGCGAACCTGGAGGACGTGCCGTCGATGGAGCTGATGACGGAGCTGCTCCGCCGCATGAAGTGCAGCTCCAAGCCCGACAAGCGCGTCATCCTCGTCGGTCCACCTGGCTGCGGAAAGGGAACACAGTCACCGCTGATTAAGGATGAATTTTGCTTGTGCCATTTAGCCACTGGTGATATGTTGAGGGCTGCAGTGGCTGCTAAAACTCCACTTGGGATTAAGGCTAAAGAAGCTATGGACAAGGGAGAGCTTGTTTCTGATGACTTGGTTGTTGGGATTATTGATGAAGCCATGAAGAAAACTTCATGCCAGAAAGGTTTTATCCTTGATGGTTTCCCTAGAACTGTTGTTCAAGCACAGAAGCTTGATGAAATGTTGGCCAAACAAGGTACTAAGATTGACAAGGTTCTAAATTTTGCAATTGATGATGCAATACTGGAAGAACGAATTACCGGTCGTTGGATCCACCCATCAAGTGGTAGATCTTATCATACAAAATTTGCTCCTCCTAAGACTCCTGGACTTGATGATGTTACTGGAGAACCCTTAATTCAAAGGAAAGATGACACAGCTGCAGTATTGAAGTCAAGGCTTGAAGCCTTCCACGTACAAACTAAGCCTGTGATTGACTACTACACCAAGAAGGGCATAGTCGCAAATCTGCATGCCGAAAAACCACCAAAGGAAGTGACTGTTGAGGTCCAGAAAGCCCTATCATAA
Protein Sequence
- >LOC_Os12g13380.1
MAANLEDVPSMELMTELLRRMKCSSKPDKRVILVGPPGCGKGTQSPLIKDEFCLCHLATGDMLRAAVAAKTPLGIKAKEAMDKGELVSDDLVVGIIDEAMKKTSCQKGFILDGFPRTVVQAQKLDEMLAKQGTKIDKVLNFAIDDAILEERITGRWIHPSSGRSYHTKFAPPKTPGLDDVTGEPLIQRKDDTAAVLKSRLEAFHVQTKPVIDYYTKKGIVANLHAEKPPKEVTVEVQKALS*