Gene Details:

Functional Descriptions:

  • Differential screening of a stage-specific cDNA library of Indica rice has been used to identify two genes expressed in pre-pollination stage panicles, namely OSIPA and OSIPK coding for proteins similar to expansins/pollen allergens and calcium-dependent protein kinases (CDPK), respectively.
  • Northern analysis and in situ hybridizations indicate that OSIPA expresses exclusively in pollen while OSIPK expresses in pollen as well as anther wall.
  • Promoter of OSIPA is active during the late stages of pollen development and remains active till the anthesis, whereas, OSIPK promoter is active to a low level in developing anther till the pollen matures.
  • Furthermore, it has been demonstrated that OsCPK29 interacts in vitro as well as in vivo with the MADS68 transcription factor which is a known regulator of pollen development.
  • OsCPK29 interacts with MADS68 to regulate pollen development in rice.
  • Therefore, phenotypic observations and molecular studies suggest that OsCPK29 is an important regulator of pollen development in rice.
  • Here, we report the functional characterization of a novel OsCPK29 from rice, which is mainly expressed during pollen maturation stages of the anther.
  • OsCPK29 knockdown rice plants exhibit reduced fertility, set fewer seeds, and produce collapsed non-viable pollen grains that do not germinate.
  • OsCPK29 exclusively localizes in the nucleus, and its N-terminal variable domain is responsible for retaining it in the nucleus.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os12g12860.1
    ATTTCCTTCCATCTCTCTCTCTCCTCCTCCTCTCTTGCCCTAGAGAGAGAGAGAGAGAGGGAGGGGGCCGCAAGAGAGAGAGAGATCGATCACCGGCCGCCCTCCGGAGCTCGCGCGCTCGTTCGTTCGTTCGTTCGATCATTCGTACGTCCACAACAATTCCGGCGACGCGACCGTCGCCGTACGTTTGCGGTGGCGCGGCAGCGTCGTCGGCGGTGAAGAAGAAGAAGAAGAAGATCGAAGAAGAGGAAGAGGATGGGCAACTGCTGCGTGTCCCGGCCGTCCGGCGCCGACAAGCGGCGGCGCTGCGGCAGCTCCACCGCCCCGCACACCCGCGGCGGCAGGCGGGTGATCGGCGCCGCCAACATGCGGTGCCTCTCCACGGTCTCCTCCGTGTCGGACGCGGCGCGCGCCGTGATGTCGAACGAGCCGGCGACCGTGCTGGGCAACAGCGGCAGCAGCGGCAACGGCGGCGTGATGGCGGCGGAGGAGATGCTGCGGCGGTACGAGATCGGGGAGGAGCTGGGTCGGGGGGAGTTCGGGGTGACGCGGCGGTGCCGGGACGCGGTGACGGGGGAGCGGCTGGCGTGCAAGTCGATCAGCAAGCGGAAGCTCCGGAGCAGCGTGGACGTGGAGGACGTGCGGCGGGAGGTGGCCATCATGCGGTCGCTGCCGGCGCACGCCAACGTGGTGAGGCTCCGGGAGGCGTTCGAGGACGCCGACGCCGTCCACCTCGTCATGGAGGTCTGCGAGGGCGGCGAGCTCTTCGACCGCATCGTCGCCCGCGGCCACTACACCGAGCGCGCCGCCGCCGCCGTCATGCGCACCATCATGGACGTCGTCCAGCACTGTCACAAGAACGGCGTCATGCATCGGGACCTCAAACCTGAGAACTTTCTGTATGCCAATGCATCTGAGAACTCCCCTCTCAAGGTCATCGACTTCGGCCTCTCAGTGTGCTTCAAACCTGGTGCAAGATTCAATGAGATTGTTGGATCTCCCTACTACATGGCACCAGAAGTCCTGAAGAGAAACTATGGACAAGAAATAGACATATGGAGTGCAGGAGTCATACTGTACATCTTGCTATGTGGTGTTCCTCCTTTCTGGGCCGAGACCGACGAAGGGATCGCACAGGCCATCATCCGGTCACATATCGACTTCCAAAGAGAGCCTTGGCCAAAGGTGTCAGATAATGCCAAGGATCTTGTAAGGAGGATGCTTGATCCAAACCCATATACTCGGTTGACGGCTCAACAGGTTCTAGAGCATCCTTGGATACAGAATGCAAGTGCAGCTCCCAACATTCCTCTGGGAGAAGCAGTCAGGTCCAGGCTGAAGCAATTCACTGTTATGAACAAGTTCAAGAAGAAGGCCTTACTTGTGGTGGCGGAGTACTTACCAACCGAAGAGCTGGATGCAATCAGGGAGCTGTTCAATATGTTGGACACCAAAAAGAAGGGGCACTTGACACTAGAAGAGCTCAGAAAGGGACTGCAAGTGATAGGGCACAACATCCATGACACAGATGTCGATATGCTCATGGAAGCTGCAGACATAGATGGAAATGGCATACTGGATTGCAAGGAGTTTGTGACAGTATCCATTCACCTGAAGAAAATCCGTAGCGATGAGCACCTTCCGAAGGTGTTCAGCTTCTTCGACAAGAACGGCAGCGGGTACATAGAGATCGAAGAGTTGAAAGAAGCCCTCTCCCCAAGAGGTGACCAGAAGTCGATCGACGACATATTTCTGGATGTTGACATAGACAAGGATGGGAAGATAAGCTACGAGGAGTTTGAGTTAATGATGTCAGCCGGAATGGACTGGAGGAATGCATCCCGGCAATACTCGAGAGCAGTTTACAACACTCTCAGCCGCAAGATATTCAAGGAAGTGTCCCTGAAGCTTGATCACAGTGGTCCACTTGTTGCAGCAGGAAAATAGCAACCAGCTGTTAACTAAGTGATGTGTTCTCTACATGCAATGCTCACTGCCTCACTCATAACTGAGAGAATGTGAAAAACATGGCGCAGTTTTCCTTGTTCGCGTATTGGTCTCCACATTTTTGGGATGCAAAGGTACCACGGGGTTCAGTGACCCTGAATGTTTCAGATGCAGATGTCAAAGAGCAGGCTTAAATACACCCTTTCTCTTTCTCTGAAAACAGAGGGAAAAAAAGAAAGAAAGAGTTGTATCCTTCTGACTTTGAAGTCACTCTCCATGTTTGTTAATGTAAAACCAGAGGTAGGCCGAATCTCATATTGTTCAACACAGGAGGCAGCAATGCCCTGTACCACTAACCGCTTTGGTGCAAATTCTCCGCGGCCGCACAGTAAGGGCCAAAAAAAGAAAGAAGCAATAATATAAGATTGTTCAATTCAACTAAAAGGGAAAAAAAGTAGTTCGCAATTGAAATTATCTGCA
CDS Sequence
  • >LOC_Os12g12860.1
    ATGGGCAACTGCTGCGTGTCCCGGCCGTCCGGCGCCGACAAGCGGCGGCGCTGCGGCAGCTCCACCGCCCCGCACACCCGCGGCGGCAGGCGGGTGATCGGCGCCGCCAACATGCGGTGCCTCTCCACGGTCTCCTCCGTGTCGGACGCGGCGCGCGCCGTGATGTCGAACGAGCCGGCGACCGTGCTGGGCAACAGCGGCAGCAGCGGCAACGGCGGCGTGATGGCGGCGGAGGAGATGCTGCGGCGGTACGAGATCGGGGAGGAGCTGGGTCGGGGGGAGTTCGGGGTGACGCGGCGGTGCCGGGACGCGGTGACGGGGGAGCGGCTGGCGTGCAAGTCGATCAGCAAGCGGAAGCTCCGGAGCAGCGTGGACGTGGAGGACGTGCGGCGGGAGGTGGCCATCATGCGGTCGCTGCCGGCGCACGCCAACGTGGTGAGGCTCCGGGAGGCGTTCGAGGACGCCGACGCCGTCCACCTCGTCATGGAGGTCTGCGAGGGCGGCGAGCTCTTCGACCGCATCGTCGCCCGCGGCCACTACACCGAGCGCGCCGCCGCCGCCGTCATGCGCACCATCATGGACGTCGTCCAGCACTGTCACAAGAACGGCGTCATGCATCGGGACCTCAAACCTGAGAACTTTCTGTATGCCAATGCATCTGAGAACTCCCCTCTCAAGGTCATCGACTTCGGCCTCTCAGTGTGCTTCAAACCTGGTGCAAGATTCAATGAGATTGTTGGATCTCCCTACTACATGGCACCAGAAGTCCTGAAGAGAAACTATGGACAAGAAATAGACATATGGAGTGCAGGAGTCATACTGTACATCTTGCTATGTGGTGTTCCTCCTTTCTGGGCCGAGACCGACGAAGGGATCGCACAGGCCATCATCCGGTCACATATCGACTTCCAAAGAGAGCCTTGGCCAAAGGTGTCAGATAATGCCAAGGATCTTGTAAGGAGGATGCTTGATCCAAACCCATATACTCGGTTGACGGCTCAACAGGTTCTAGAGCATCCTTGGATACAGAATGCAAGTGCAGCTCCCAACATTCCTCTGGGAGAAGCAGTCAGGTCCAGGCTGAAGCAATTCACTGTTATGAACAAGTTCAAGAAGAAGGCCTTACTTGTGGTGGCGGAGTACTTACCAACCGAAGAGCTGGATGCAATCAGGGAGCTGTTCAATATGTTGGACACCAAAAAGAAGGGGCACTTGACACTAGAAGAGCTCAGAAAGGGACTGCAAGTGATAGGGCACAACATCCATGACACAGATGTCGATATGCTCATGGAAGCTGCAGACATAGATGGAAATGGCATACTGGATTGCAAGGAGTTTGTGACAGTATCCATTCACCTGAAGAAAATCCGTAGCGATGAGCACCTTCCGAAGGTGTTCAGCTTCTTCGACAAGAACGGCAGCGGGTACATAGAGATCGAAGAGTTGAAAGAAGCCCTCTCCCCAAGAGGTGACCAGAAGTCGATCGACGACATATTTCTGGATGTTGACATAGACAAGGATGGGAAGATAAGCTACGAGGAGTTTGAGTTAATGATGTCAGCCGGAATGGACTGGAGGAATGCATCCCGGCAATACTCGAGAGCAGTTTACAACACTCTCAGCCGCAAGATATTCAAGGAAGTGTCCCTGAAGCTTGATCACAGTGGTCCACTTGTTGCAGCAGGAAAATAG
Protein Sequence
  • >LOC_Os12g12860.1
    MGNCCVSRPSGADKRRRCGSSTAPHTRGGRRVIGAANMRCLSTVSSVSDAARAVMSNEPATVLGNSGSSGNGGVMAAEEMLRRYEIGEELGRGEFGVTRRCRDAVTGERLACKSISKRKLRSSVDVEDVRREVAIMRSLPAHANVVRLREAFEDADAVHLVMEVCEGGELFDRIVARGHYTERAAAAVMRTIMDVVQHCHKNGVMHRDLKPENFLYANASENSPLKVIDFGLSVCFKPGARFNEIVGSPYYMAPEVLKRNYGQEIDIWSAGVILYILLCGVPPFWAETDEGIAQAIIRSHIDFQREPWPKVSDNAKDLVRRMLDPNPYTRLTAQQVLEHPWIQNASAAPNIPLGEAVRSRLKQFTVMNKFKKKALLVVAEYLPTEELDAIRELFNMLDTKKKGHLTLEELRKGLQVIGHNIHDTDVDMLMEAADIDGNGILDCKEFVTVSIHLKKIRSDEHLPKVFSFFDKNGSGYIEIEELKEALSPRGDQKSIDDIFLDVDIDKDGKISYEEFELMMSAGMDWRNASRQYSRAVYNTLSRKIFKEVSLKLDHSGPLVAAGK*