Gene Details:
- MSU gene ID: LOC_Os12g10184
- RAPdb gene ID: None
- Gene Symbol: PGL12
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- We also showed that the transcript levels of several nuclear- and plastid-encoded genes associated with chloroplast development and photosynthesis were significantly altered in PGL12 mutants.
- Our results indicate that PGL12 is a new PLS-type PPR protein required for proper chloroplast development and 16S rRNA processing in rice.
- The PGL12 mutant had significantly reduced chlorophyll contents and increased sensitivity to changes in temperature.
- In this study, we identified and characterized a rice mutant, pale-green leaf12 (PGL12); at the seedling stage, PGL12 mutants had yellow-green leaves, which gradually turned pale green as the plants grew.
- Map-based cloning of PGL12, including a transgenic complementation test, confirmed the presence of a base substitution (C to T), generating a stop codon, within LOC_Os12g10184 in the PGL12 mutant.
Function-related keywords:
- chloroplast , temperature , seedling , development , map-based-cloning , photosynthesis , R-protein , chloroplast-development
Literature:
- PALE-GREEN LEAF12 Encodes a Novel Pentatricopeptide Repeat Protein Required for Chloroplast Development and 16S rRNA Processing in Rice . DOI: 10.1093/pcp/pcy229 ; PMID: 30508149
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os12g10184.1
ATGGCGATGGCGTCCTCCTCCCTCGCCGCCGCAAACCACCCCCTCCTCCCCTCCAAGCCGCTCTCCTGCCCCAAGCCCCTCACCCGCGCGACGCCCCTCTCCCGCGCGCTCCACGCCGGCGCCGCCGCGGCCGCGCGGGGCGCTTCGTCGTCGCAGGCGGAGCTCCGGCCGGACTCCAAGAACGCGCCCGCGCTGTCCGCGGAGCTCCGGAGGCTGGCGCGCGTCGGGAGGCTCCCCTCCGCGCTCGCCCTCCTCGACCACCTCTCCCACCGCGGTGTCCCCGCCACGGCCTCGGCCTTCGCCGCGCTGCTCACCGCGTGCCGGTCGCTCGCCCACGCGCGCCAGGTCCACGCGCACCTCCGCGTCCACGGCCTCGACTCCAACGAGTTCCTGCTCGCCAGGCTCGTCGAGCTCTACCTCGCGCTCGGCGCCCGGGAGGACGCGCGCAAGGTGCTCGACGGGTTGCCCCGGGCGAGCTCCTTCTCGTGGAACGCGCTCCTCCACGGGCACGTGCGCCGGGGACGCGGCCAGGCGGGCGCCGCCGTCGTGGATGGGTTCGCAGAGATGCGCGCCGCGGGCGCCGACGCCAACGAGTACACGTATGGCTGCGTCCTCAAGTCCATCTCCGGGAGCGCCGCGCCGTCCATGGCCATGGCCACCGCCACGCACGCGATGCTGATCAAGAACGCCTACGCGGGCGCTCCCAGGATGCTCATGACGGGGCTCATGGACGTGTACTTCAAGTGCGGGAAGGTGAAGCCTGCGGTTATGGTGTTCGAGGAAATGCCAGAGAGGGATGTGGTTGCGTGGGGTGCAGTGATTGCCGGGTTTGCGCATAAGGGGATGAAGAGGGAGGCGCTGGAGCATTTCCGGTGGATGGTGGAGGATGGGATCAAGGTGAACTCTGTGGTGCTTACGTCAGTTGTGCCTGTCATCGGCGAGTTGCGTGCCTGGAACTTGGGTAGGGAGATCCATGGTTTTGTGCTGAAGAAGTTCCAACATCGTAAGGATGTTGGAAACATTCAGGCAGGGTTGGTAGATATGTACTGCAAGTGTGGCGATATGGTCTCTGGGAGACGGGTGTTCTATAGCGCTAAGAAGAGGAATGTTGTCTCATGGACGGCACTGATGTCTGGCTATGCATCCAATGGCAGGCCTGACCAGGCGCTGAGGTGCATACTTTGGATGCAACAAGAAGGAATCAGACCGGATCTTATAGCCGTGGGGACTGTGCTCCCAGTATGTACGAAGTTGAAAGCATTGAGGGAAGGGAAAGAGCTTCACGCATATGCCTTGCGGAGGTGGTTCCTGCCAAATATTTCCTTGTGCACTTCACTGATCACTATGTATGGTACGTGCAGTCACTCGGATTACTCTCAACGGGTGTTCCATGTCATGGACAAGAAGACTGTACAAGCTTGGACAGCATTGGTCGATGCGTACCTAAAGAATGGTGATCCTTCAACTGCTGTTGATGTGTTCAGATCAATGCTGCTCTCAAACCGCCGGCCTGATGCTGTTGCAATCAGCAGGATGTTGAGTGCCTGCCGTGACATTGGGGCATCAAAACTTGGCAAGGAATTGCATGGTCAAGCGTTAAAGTTGCGGATGGAACCTCTCCCCCTAGTTGCAGCAGGGCTAGTTAGCATGTATGGCACATGTGGAGACCTCAAGGCAGCGCAACGGGTTTTCAACCGGATTGAGTCCAAGGGATCCCTTACTTGCACTGCCATAATAGAAGCTTACGCAATCAACCAGAGACACAAGGAGGCACTGGAACTCTTTGCTTGGATGCTGTCAAACAGGTTTGTTCCAAATAATGGCACCTTTGATGTGTTGCTGAGAATCTGTGAAGCGGCAGGGTTGCATGATGAAGCTCTTCAGATTTTCAACTCCATGGTGCAAGAATACAATCTGGAAGCGTCTGAACATAATTTTGACTGCATTATACGGCTTCTCACTGCTGCAGACAGGACTTCTGAAGCACATCGGTTTGCTGATCTGAAAGCTACTCTGTTTAATTTATCAACTCCCTCTTTGAATTGCTAACAGCAGTAAATCTGTATATAACGCCACATTTGATTTGTTGAAATGACTGATACTCCTTTTCTATTGTCTGAAAAGGTAAGCTTGGTATTCGTTTTTCAAGAAAAGATTAAATATCAATGGCATGCTAAAGTTAGTTA
CDS Sequence
- >LOC_Os12g10184.1
ATGGCGATGGCGTCCTCCTCCCTCGCCGCCGCAAACCACCCCCTCCTCCCCTCCAAGCCGCTCTCCTGCCCCAAGCCCCTCACCCGCGCGACGCCCCTCTCCCGCGCGCTCCACGCCGGCGCCGCCGCGGCCGCGCGGGGCGCTTCGTCGTCGCAGGCGGAGCTCCGGCCGGACTCCAAGAACGCGCCCGCGCTGTCCGCGGAGCTCCGGAGGCTGGCGCGCGTCGGGAGGCTCCCCTCCGCGCTCGCCCTCCTCGACCACCTCTCCCACCGCGGTGTCCCCGCCACGGCCTCGGCCTTCGCCGCGCTGCTCACCGCGTGCCGGTCGCTCGCCCACGCGCGCCAGGTCCACGCGCACCTCCGCGTCCACGGCCTCGACTCCAACGAGTTCCTGCTCGCCAGGCTCGTCGAGCTCTACCTCGCGCTCGGCGCCCGGGAGGACGCGCGCAAGGTGCTCGACGGGTTGCCCCGGGCGAGCTCCTTCTCGTGGAACGCGCTCCTCCACGGGCACGTGCGCCGGGGACGCGGCCAGGCGGGCGCCGCCGTCGTGGATGGGTTCGCAGAGATGCGCGCCGCGGGCGCCGACGCCAACGAGTACACGTATGGCTGCGTCCTCAAGTCCATCTCCGGGAGCGCCGCGCCGTCCATGGCCATGGCCACCGCCACGCACGCGATGCTGATCAAGAACGCCTACGCGGGCGCTCCCAGGATGCTCATGACGGGGCTCATGGACGTGTACTTCAAGTGCGGGAAGGTGAAGCCTGCGGTTATGGTGTTCGAGGAAATGCCAGAGAGGGATGTGGTTGCGTGGGGTGCAGTGATTGCCGGGTTTGCGCATAAGGGGATGAAGAGGGAGGCGCTGGAGCATTTCCGGTGGATGGTGGAGGATGGGATCAAGGTGAACTCTGTGGTGCTTACGTCAGTTGTGCCTGTCATCGGCGAGTTGCGTGCCTGGAACTTGGGTAGGGAGATCCATGGTTTTGTGCTGAAGAAGTTCCAACATCGTAAGGATGTTGGAAACATTCAGGCAGGGTTGGTAGATATGTACTGCAAGTGTGGCGATATGGTCTCTGGGAGACGGGTGTTCTATAGCGCTAAGAAGAGGAATGTTGTCTCATGGACGGCACTGATGTCTGGCTATGCATCCAATGGCAGGCCTGACCAGGCGCTGAGGTGCATACTTTGGATGCAACAAGAAGGAATCAGACCGGATCTTATAGCCGTGGGGACTGTGCTCCCAGTATGTACGAAGTTGAAAGCATTGAGGGAAGGGAAAGAGCTTCACGCATATGCCTTGCGGAGGTGGTTCCTGCCAAATATTTCCTTGTGCACTTCACTGATCACTATGTATGGTACGTGCAGTCACTCGGATTACTCTCAACGGGTGTTCCATGTCATGGACAAGAAGACTGTACAAGCTTGGACAGCATTGGTCGATGCGTACCTAAAGAATGGTGATCCTTCAACTGCTGTTGATGTGTTCAGATCAATGCTGCTCTCAAACCGCCGGCCTGATGCTGTTGCAATCAGCAGGATGTTGAGTGCCTGCCGTGACATTGGGGCATCAAAACTTGGCAAGGAATTGCATGGTCAAGCGTTAAAGTTGCGGATGGAACCTCTCCCCCTAGTTGCAGCAGGGCTAGTTAGCATGTATGGCACATGTGGAGACCTCAAGGCAGCGCAACGGGTTTTCAACCGGATTGAGTCCAAGGGATCCCTTACTTGCACTGCCATAATAGAAGCTTACGCAATCAACCAGAGACACAAGGAGGCACTGGAACTCTTTGCTTGGATGCTGTCAAACAGGTTTGTTCCAAATAATGGCACCTTTGATGTGTTGCTGAGAATCTGTGAAGCGGCAGGGTTGCATGATGAAGCTCTTCAGATTTTCAACTCCATGGTGCAAGAATACAATCTGGAAGCGTCTGAACATAATTTTGACTGCATTATACGGCTTCTCACTGCTGCAGACAGGACTTCTGAAGCACATCGGTTTGCTGATCTGAAAGCTACTCTGTTTAATTTATCAACTCCCTCTTTGAATTGCTAA
Protein Sequence
- >LOC_Os12g10184.1
MAMASSSLAAANHPLLPSKPLSCPKPLTRATPLSRALHAGAAAAARGASSSQAELRPDSKNAPALSAELRRLARVGRLPSALALLDHLSHRGVPATASAFAALLTACRSLAHARQVHAHLRVHGLDSNEFLLARLVELYLALGAREDARKVLDGLPRASSFSWNALLHGHVRRGRGQAGAAVVDGFAEMRAAGADANEYTYGCVLKSISGSAAPSMAMATATHAMLIKNAYAGAPRMLMTGLMDVYFKCGKVKPAVMVFEEMPERDVVAWGAVIAGFAHKGMKREALEHFRWMVEDGIKVNSVVLTSVVPVIGELRAWNLGREIHGFVLKKFQHRKDVGNIQAGLVDMYCKCGDMVSGRRVFYSAKKRNVVSWTALMSGYASNGRPDQALRCILWMQQEGIRPDLIAVGTVLPVCTKLKALREGKELHAYALRRWFLPNISLCTSLITMYGTCSHSDYSQRVFHVMDKKTVQAWTALVDAYLKNGDPSTAVDVFRSMLLSNRRPDAVAISRMLSACRDIGASKLGKELHGQALKLRMEPLPLVAAGLVSMYGTCGDLKAAQRVFNRIESKGSLTCTAIIEAYAINQRHKEALELFAWMLSNRFVPNNGTFDVLLRICEAAGLHDEALQIFNSMVQEYNLEASEHNFDCIIRLLTAADRTSEAHRFADLKATLFNLSTPSLNC*