Gene Details:

Functional Descriptions:

  • We isolated a pollen-preferential gene, RICE IMMATURE POLLEN 1 (RIP1), from a T-DNA insertional population of japonica rice that was trapped by a promoterless beta-glucuronidase (GUS) gene.
  • Semi-quantitative reverse transcription-PCR (RT-PCR) analyses confirmed that the RIP1 transcript was abundant at the late stages of pollen development.
  • Microscopic analysis demonstrated that development in the RIP1 pollen was delayed, starting at the early vacuolated stage.
  • These results indicate that RIP1 is necessary for pollen maturation and germination.
  • Therefore, RIP1 is probably a nuclear protein that may form a functional complex with other proteins and carry out essential cellular and developmental roles during the late stage of pollen formation.
  • Rice Immature Pollen 1 (RIP1) is a regulator of late pollen development.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os12g03822.1
    TCCAAGTTCCAACCTCCGCCCTCTCTTCTCTTCTCCTCTCTCCTTCTCCCCGAGCTGCCCCTCTTCTCTTCCTCCGATGGGTCGCAAGATCAAAGTCAAGAAGAAGAAGGCATCGAGCAAGAAAGCCGAAGCTTCATCGTCTAGGGTGCCTTCTTGCCCAGCCAAGGTATGGCAACCTGGTGTGGATACACTGGAAGAAGGGGAGGAGCTCCAGTTCGATCCTCAGGCTTACAATTATCTCAGAGGGTTTAACATTGGCTGGCCGTGCTTGAGTTTCGATGTTGTGCGGGATCAACTTGGACTCGTGCGATCGGAATTCCCACATACATTATATGGCGTTGCTGGAACTCAGGCTGAGAGGGCTTCATGGAATTATATAGGCATTTTCAAGATTTGCAACATAAATGGGAAGAAGCGGGAACCTATACCAGCATCAGCAATTGATGGCGACAGTGATATGGATAGTGAAAGTAGTAGTGATGAAGAAGATGAAGCAGCTAATGAAGACACAATGCCCATTTTACATTTAAAAAAGGTGGCTCATGCGGGGTGTGTAAATCGTATTCGCTCTATGAATCAGGAACCACATATATGTGCAACATGGGGAGACACAGGTCATGTTCAGGTGTGGGACTTCAGCTCCTTCCTTAATTCATTAGCAGAGTCTGGAGCAGTTGCACACAACGAAGACGACAGAATCCATAATCACGTACCTGTGAAAATATTTGGTGGCCATAAAGATGAAGGCTATGCAATTGATTGGAGTCCGCTTGTTACTGGAAGACTTGTTTCTGGTGATTGCAATAAATGCATCCACCTCTGGGAGCCAACTTCAAACAGTTGGAATGTCGATACAAATCCATTTGTAGGACACACTGCAAGTGTTGAAGATTTGCAGTGGAGTCCCACAGAAGCCGACATATTTGCCTCTTGTTCAGCCGACAGAACAATATCTATCTGGGATATACGGACAGGGAAGAAACCTTGTATTTCTGTTAGGGCTCACAATGCTGACGTGAATGTCATCTCATGGAACCGGCTCGCTAGCTGTATGATAGCTTCAGGATGCGATGATGGAAGTTTCTCAATTCGTGATCTTAGATTAATCAAGGATGACTCCTTGGTCGCCCATTTTGAATACCACAAGCACCCGATTACATCTGTGGAATGGAGCCCACATGAACCGTCAACACTAGCTGTATCATCAGCTGACCATCAACTGACAATTTGGGATCTTTCATTGGAAAAAGATGCTGAAGAGGAGGCAGAGTTCAGGGCAAGGATGAGAGAACAGGCTGATGCCCCTGAAGATTTACCCCCACAACTTCTCTTTGTTCATCAGGGCCAGAAAGACTTGAAAGAATTGCACTGGCATCCACAGATACCTAGTATGATCATATCTACGGCAGCTGATGGATTCAATATGTTGATGCCCAGCAACATCGATACAACCATTCGAGAAGCTGACGCATAGATTTCTACAGTAGTTGTTTATCTGAATCAAGGTTTCATGGGTTTTTTTTTTTTTTGAAGTGGATGAGTGTAATGATACTGCATGTCTACAAGTATCAGTTTTTGATAGCTTGTATCCTAGTTTCGTTGTCATTCATAGTGGTAGGTTCTCAGTTTTGCACTGATGTCGCCCGAATGAGGATAATCAAAAGTATCTCGATCAACTGAAATGACATGTTATATTCTACAAGATAAGATTAATAATTGTGCATCTCTGTGTTTGCATTAGGTAATATTCACCTCGATCGTTTGTATATTTTTGCCTGATAATTAAGTTGGATATTAGGTCCATCGGCCAG
  • >LOC_Os12g03822.2
    TCCAAGTTCCAACCTCCGCCCTCTCTTCTCTTCTCCTCTCTCCTTCTCCCCGAGCTGCCCCTCTTCTCTTCCTCCGATGGGTCGCAAGATCAAAGTCAAGAAGAAGAAGGCATCGAGCAAGAAAGCCGAAGCTTCATCGTCTAGGGTGCCTTCTTGCCCAGCCAAGGTATGGCAACCTGGTGTGGATACACTGGAAGAAGGGGAGGAGCTCCAGTTCGATCCTCAGGCTTACAATTATCTCAGAGGGTTTAACATTGGCTGGCCGTGCTTGAGTTTCGATGTTGTGCGGGATCAACTTGGACTCGTGCGATCGGAATTCCCACATACATTATATGGCGTTGCTGGAACTCAGGCTGAGAGGGCTTCATGGAATTATATAGGCATTTTCAAGATTTGCAACATAAATGGGAAGAAGCGGGAACCTATACCAGCATCAGCAATTGATGGCGACAGTGATATGGATAGTGAAAGTAGTAGTGATGAAGAAGATGAAGCAGCTAATGAAGACACAATGCCCATTTTACATTTAAAAAAGGTGGCTCATGCGGGGTGTGTAAATCGTATTCGCTCTATGAATCAGGAACCACATATATGTGCAACATGGGGAGACACAGGTCATGTTCAGGTGTGGGACTTCAGCTCCTTCCTTAATTCATTAGCAGAGTCTGGAGCAGTTGCACACAACGAAGACGACAGAATCCATAATCACGTACCTGTGAAAATATTTGGTGGCCATAAAGATGAAGGCTATGCAATTGATTGGAGTCCGCTTGTTACTGGAAGACTTGTTTCTGGTGATTGCAATAAATGCATCCACCTCTGGGAGCCAACTTCAAACAGTTGGAATGTCGATACAAATCCATTTGTAGGACACACTGCAAGTGTTGAAGATTTGCAGTGGAGTCCCACAGAAGCCGACATATTTGCCTCTTGTTCAGCCGACAGAACAATATCTATCTGGGATATACGGACAGGGAAGAAACCTTGTATTTCTGTTAGGGCTCACAATGCTGACGTGAATGTCATCTCATGGAACCGGCTCGCTAGCTGTATGATAGCTTCAGGATGCGATGATGGAAGTTTCTCAATTCGTGATCTTAGATTAATCAAGGATGACTCCTTGGTCGCCCATTTTGAATACCACAAGCACCCGATTACATCTGTGGAATGGAGCCCACATGAACCGTCAACACTAGCTGTATCATCAGCTGACCATCAACTGACAATTTGGGATCTTTCATTGGAAAAAGATGCTGAAGAGGAGGCAGAGTTCAGGGCAAGGATGAGAGAACAGGCTGATGCCCCTGAAGATTTACCCCCACAACTTCTCTTTGTTCATCAGGGCCAGAAAGACTTGAAAGAATTGCACTGGCATCCACAGATACCTAGTATGATCATATCTACGGCAGCTGATGGATTCAATATGTTGATGCCCAGCAACATCGATACAACCATTCGAGAAGCTGACGCATAGATTTCTACAGTAGTTGTTTATCTGAATCAAGGTTTCATGGGTTTTTTTTTTTTTTGAAGTGGATGAGTGTAATGATACTGCATGTCTACAAGTATCAGTTTTTGATAGCTTGTATCCTAGTTTCGTTGTCATTCATAGTGGTAGGTTCTCAGTTTTGCACTGATGTCGCCCGAATGAGGATAATCAAAAGTATCTCGATCAACTGAAATGACATGTTATATTCTACAAGATAAGATTAATAATTGTGCATCTCTGTGTTTGCATTAGGTAATATTCACCTCGATCGTTTGTATATTTTTGCCTGATAATTAAGTTGGATATTAGGTCCATCGGCCAG
CDS Sequence
  • >LOC_Os12g03822.1
    ATGGGTCGCAAGATCAAAGTCAAGAAGAAGAAGGCATCGAGCAAGAAAGCCGAAGCTTCATCGTCTAGGGTGCCTTCTTGCCCAGCCAAGGTATGGCAACCTGGTGTGGATACACTGGAAGAAGGGGAGGAGCTCCAGTTCGATCCTCAGGCTTACAATTATCTCAGAGGGTTTAACATTGGCTGGCCGTGCTTGAGTTTCGATGTTGTGCGGGATCAACTTGGACTCGTGCGATCGGAATTCCCACATACATTATATGGCGTTGCTGGAACTCAGGCTGAGAGGGCTTCATGGAATTATATAGGCATTTTCAAGATTTGCAACATAAATGGGAAGAAGCGGGAACCTATACCAGCATCAGCAATTGATGGCGACAGTGATATGGATAGTGAAAGTAGTAGTGATGAAGAAGATGAAGCAGCTAATGAAGACACAATGCCCATTTTACATTTAAAAAAGGTGGCTCATGCGGGGTGTGTAAATCGTATTCGCTCTATGAATCAGGAACCACATATATGTGCAACATGGGGAGACACAGGTCATGTTCAGGTGTGGGACTTCAGCTCCTTCCTTAATTCATTAGCAGAGTCTGGAGCAGTTGCACACAACGAAGACGACAGAATCCATAATCACGTACCTGTGAAAATATTTGGTGGCCATAAAGATGAAGGCTATGCAATTGATTGGAGTCCGCTTGTTACTGGAAGACTTGTTTCTGGTGATTGCAATAAATGCATCCACCTCTGGGAGCCAACTTCAAACAGTTGGAATGTCGATACAAATCCATTTGTAGGACACACTGCAAGTGTTGAAGATTTGCAGTGGAGTCCCACAGAAGCCGACATATTTGCCTCTTGTTCAGCCGACAGAACAATATCTATCTGGGATATACGGACAGGGAAGAAACCTTGTATTTCTGTTAGGGCTCACAATGCTGACGTGAATGTCATCTCATGGAACCGGCTCGCTAGCTGTATGATAGCTTCAGGATGCGATGATGGAAGTTTCTCAATTCGTGATCTTAGATTAATCAAGGATGACTCCTTGGTCGCCCATTTTGAATACCACAAGCACCCGATTACATCTGTGGAATGGAGCCCACATGAACCGTCAACACTAGCTGTATCATCAGCTGACCATCAACTGACAATTTGGGATCTTTCATTGGAAAAAGATGCTGAAGAGGAGGCAGAGTTCAGGGCAAGGATGAGAGAACAGGCTGATGCCCCTGAAGATTTACCCCCACAACTTCTCTTTGTTCATCAGGGCCAGAAAGACTTGAAAGAATTGCACTGGCATCCACAGATACCTAGTATGATCATATCTACGGCAGCTGATGGATTCAATATGTTGATGCCCAGCAACATCGATACAACCATTCGAGAAGCTGACGCATAG
  • >LOC_Os12g03822.2
    ATGGGTCGCAAGATCAAAGTCAAGAAGAAGAAGGCATCGAGCAAGAAAGCCGAAGCTTCATCGTCTAGGGTGCCTTCTTGCCCAGCCAAGGTATGGCAACCTGGTGTGGATACACTGGAAGAAGGGGAGGAGCTCCAGTTCGATCCTCAGGCTTACAATTATCTCAGAGGGTTTAACATTGGCTGGCCGTGCTTGAGTTTCGATGTTGTGCGGGATCAACTTGGACTCGTGCGATCGGAATTCCCACATACATTATATGGCGTTGCTGGAACTCAGGCTGAGAGGGCTTCATGGAATTATATAGGCATTTTCAAGATTTGCAACATAAATGGGAAGAAGCGGGAACCTATACCAGCATCAGCAATTGATGGCGACAGTGATATGGATAGTGAAAGTAGTAGTGATGAAGAAGATGAAGCAGCTAATGAAGACACAATGCCCATTTTACATTTAAAAAAGGTGGCTCATGCGGGGTGTGTAAATCGTATTCGCTCTATGAATCAGGAACCACATATATGTGCAACATGGGGAGACACAGGTCATGTTCAGGTGTGGGACTTCAGCTCCTTCCTTAATTCATTAGCAGAGTCTGGAGCAGTTGCACACAACGAAGACGACAGAATCCATAATCACGTACCTGTGAAAATATTTGGTGGCCATAAAGATGAAGGCTATGCAATTGATTGGAGTCCGCTTGTTACTGGAAGACTTGTTTCTGGTGATTGCAATAAATGCATCCACCTCTGGGAGCCAACTTCAAACAGTTGGAATGTCGATACAAATCCATTTGTAGGACACACTGCAAGTGTTGAAGATTTGCAGTGGAGTCCCACAGAAGCCGACATATTTGCCTCTTGTTCAGCCGACAGAACAATATCTATCTGGGATATACGGACAGGGAAGAAACCTTGTATTTCTGTTAGGGCTCACAATGCTGACGTGAATGTCATCTCATGGAACCGGCTCGCTAGCTGTATGATAGCTTCAGGATGCGATGATGGAAGTTTCTCAATTCGTGATCTTAGATTAATCAAGGATGACTCCTTGGTCGCCCATTTTGAATACCACAAGCACCCGATTACATCTGTGGAATGGAGCCCACATGAACCGTCAACACTAGCTGTATCATCAGCTGACCATCAACTGACAATTTGGGATCTTTCATTGGAAAAAGATGCTGAAGAGGAGGCAGAGTTCAGGGCAAGGATGAGAGAACAGGCTGATGCCCCTGAAGATTTACCCCCACAACTTCTCTTTGTTCATCAGGGCCAGAAAGACTTGAAAGAATTGCACTGGCATCCACAGATACCTAGTATGATCATATCTACGGCAGCTGATGGATTCAATATGTTGATGCCCAGCAACATCGATACAACCATTCGAGAAGCTGACGCATAG
Protein Sequence
  • >LOC_Os12g03822.1
    MGRKIKVKKKKASSKKAEASSSRVPSCPAKVWQPGVDTLEEGEELQFDPQAYNYLRGFNIGWPCLSFDVVRDQLGLVRSEFPHTLYGVAGTQAERASWNYIGIFKICNINGKKREPIPASAIDGDSDMDSESSSDEEDEAANEDTMPILHLKKVAHAGCVNRIRSMNQEPHICATWGDTGHVQVWDFSSFLNSLAESGAVAHNEDDRIHNHVPVKIFGGHKDEGYAIDWSPLVTGRLVSGDCNKCIHLWEPTSNSWNVDTNPFVGHTASVEDLQWSPTEADIFASCSADRTISIWDIRTGKKPCISVRAHNADVNVISWNRLASCMIASGCDDGSFSIRDLRLIKDDSLVAHFEYHKHPITSVEWSPHEPSTLAVSSADHQLTIWDLSLEKDAEEEAEFRARMREQADAPEDLPPQLLFVHQGQKDLKELHWHPQIPSMIISTAADGFNMLMPSNIDTTIREADA*
  • >LOC_Os12g03822.2
    MGRKIKVKKKKASSKKAEASSSRVPSCPAKVWQPGVDTLEEGEELQFDPQAYNYLRGFNIGWPCLSFDVVRDQLGLVRSEFPHTLYGVAGTQAERASWNYIGIFKICNINGKKREPIPASAIDGDSDMDSESSSDEEDEAANEDTMPILHLKKVAHAGCVNRIRSMNQEPHICATWGDTGHVQVWDFSSFLNSLAESGAVAHNEDDRIHNHVPVKIFGGHKDEGYAIDWSPLVTGRLVSGDCNKCIHLWEPTSNSWNVDTNPFVGHTASVEDLQWSPTEADIFASCSADRTISIWDIRTGKKPCISVRAHNADVNVISWNRLASCMIASGCDDGSFSIRDLRLIKDDSLVAHFEYHKHPITSVEWSPHEPSTLAVSSADHQLTIWDLSLEKDAEEEAEFRARMREQADAPEDLPPQLLFVHQGQKDLKELHWHPQIPSMIISTAADGFNMLMPSNIDTTIREADA*