Gene Details:
- MSU gene ID: LOC_Os12g03150
- RAPdb gene ID: Os12g0125000
- Gene Symbol: OsMYB60
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Downregulation of TL by RNA interference (RNAi) and overexpression of OsMYB60 resulted in twisted leaf blades in transgenic rice.
- This suggests that TL may play a cis-regulatory role on OsMYB60 in leaf morphological development.
- We further discovered that a C2H2 transcription factor, OsZFP7, is an OsMYB60 binding partner and involved in leaf development.
- Quantitative reverse-transcription PCR showed that the loss-of-function of OsMYB60 led to downregulation of wax biosynthetic genes, leading to reduced amounts of total wax components on leaf surfaces under normal conditions.
- Taken together, these results demonstrate that OsMYB60 contributes to enhancing rice resilience to drought stress by promoting cuticular wax biosynthesis on leaf surfaces.
- Here, we show that the MYB transcription factor OsMYB60 positively regulates cuticular wax biosynthesis and this helps rice (Oryza sativa) plants tolerate drought stress.
- Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis.
- Compared with the wild type (japonica cultivar 'Dongjin'), OsMYB60 null mutants (OsMYB60-1 and OsMYB60-2) exhibited increased drought sensitivity, with higher chlorophyll leaching and faster rates of water loss.
Function-related keywords:
- leaf , transcription-factor , leaf-development , development , drought , stress , chlorophyll , drought-stress , drought-sensitivity , water-loss , cuticular-wax-biosynthesis , wax-biosynthesis
Literature:
- A novel antisense long noncoding RNA, TWISTED LEAF, maintains leaf blade flattening by regulating its associated sense R2R3-MYB gene in rice . DOI: 10.1111/nph.15023 ; PMID: 29411384
- Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis . DOI: 10.1111/tpj.15947 ; PMID: 35984735
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os12g03150.1
TGTTGACAGAAAGAGAAAGCATCCTACATACAGACAGATCGATCGATCGATCGATAACCAGATCAACTGACTTGCTAGAAAGTGAAAGTAAATCATCTCATCGATCGATCAGAAAGTGGCTTACATCAGAGATAGCAAGTGAAAAGGAGCTCACTCACTCCCAAAGTCCCCAGTCTCCTAGCTATATATATCTCTACACTCAAGCACACTGTCGGAAGCTCAGGTAGCACAGAGAGAGACGACCGATCGATCGATCAGCTAGCTGCAGCTTCCAGAAAGAGTAGTTAGCTCGATCGATCGATCCGGGAAGGATCAGAGTTAATTAGTGAGTGAGGAATTGTAGCCATGGGCCGGCCGCCGTGCTGCGACAAGGAGGGGATCAAGAAGGGGCCATGGACGCCCGAGGAGGACATCATCCTCGTCTCCTACATCCAAGAACACGGCCCCGGCAACTGGAGATCGGTTCCCATCAACACTGGGCTGATGAGGTGCAGCAAGAGCTGCAGGCTGCGGTGGACGAACTACCTGCGGCCGGGGATAAAGCGCGGCAACTTCACGGCGCACGAGGAGGGGATCATCGTCCACCTCCAGTCGCTTCTCGGCAACCGCTGGGCCGCCATCGCCTCCTACCTCCCCCAGCGCACCGACAACGACATCAAGAACTACTGGAACACCCACCTCAAGAAGAAGCTCGCCGCCGCCGCCAACTCCACTTCATCCTCCAACCGCCACCCCATCTTCGCCGACGCCACCTTCCCCTCCGCCGCCGGCGGTAGCTACACGGTGAGCAGCAACTCCGATGTCACCCAGATGGCAGCCATTGCTAGGCGCTCCCCCTTCGCCGACTGCCCATCCTCCTCATACGCCTCCAGCATGGACAACATCTCCAAGCTGCTCGACGGCTTCATGAAGACCAACTCCCCGTCGCCGCCGCCGCCACCGCTGCAGCACTATGACAGCGGCTATTACGACGACGTCAAGCCCGCCGTCGATGTCGGCGGCAACCCCTTGCTGTCGTCCTTCGACTGCATGTCCGGCGCCGACCTGGACTGCTGCTTCGACGTTCATCAGCAGCACCAGCAGCAGCAGCCGGCGTCGTTCATGGAGTACGGCGGCTATGGCGGCGGCTACGGCGACGAGAGCAAGCAGCAGCTGATGAACCAGGCAGCGCCGCCGCTGTCATCGATCGAGAAGTGGCTGTTCGACGAGGCGGCGGCGGAGCAGGTCGCCGACCTCATGGACCTCTCCGACGGTTGCTGCTCCGTCCCAATGATGTTTTAATTAGCTCAAGTAATGAAAGAAAACACTGTCACATTCGATACGTATACTGTGCACATCAATAATTAATTTTACATTAATTAGCTAGATGCATGTCTAGGTTAAACTATTGTTGTTACTATACTTGAAGCTAGAAGATTTGTTTTATTTGTAGTTTTGGATGTGTGACCGGTTGTACGTGTGTCACTGGCATCAAACGTCAGCACAAGAATAAGAGATATCAACAGGAATTAACAGGTGTGATTATATATGTAAGCATGATGTAACTCATCAAGATTATTTCTTATTCATTTTTTCGAATGAATTCATAAAATTATTTCC
CDS Sequence
- >LOC_Os12g03150.1
ATGGGCCGGCCGCCGTGCTGCGACAAGGAGGGGATCAAGAAGGGGCCATGGACGCCCGAGGAGGACATCATCCTCGTCTCCTACATCCAAGAACACGGCCCCGGCAACTGGAGATCGGTTCCCATCAACACTGGGCTGATGAGGTGCAGCAAGAGCTGCAGGCTGCGGTGGACGAACTACCTGCGGCCGGGGATAAAGCGCGGCAACTTCACGGCGCACGAGGAGGGGATCATCGTCCACCTCCAGTCGCTTCTCGGCAACCGCTGGGCCGCCATCGCCTCCTACCTCCCCCAGCGCACCGACAACGACATCAAGAACTACTGGAACACCCACCTCAAGAAGAAGCTCGCCGCCGCCGCCAACTCCACTTCATCCTCCAACCGCCACCCCATCTTCGCCGACGCCACCTTCCCCTCCGCCGCCGGCGGTAGCTACACGGTGAGCAGCAACTCCGATGTCACCCAGATGGCAGCCATTGCTAGGCGCTCCCCCTTCGCCGACTGCCCATCCTCCTCATACGCCTCCAGCATGGACAACATCTCCAAGCTGCTCGACGGCTTCATGAAGACCAACTCCCCGTCGCCGCCGCCGCCACCGCTGCAGCACTATGACAGCGGCTATTACGACGACGTCAAGCCCGCCGTCGATGTCGGCGGCAACCCCTTGCTGTCGTCCTTCGACTGCATGTCCGGCGCCGACCTGGACTGCTGCTTCGACGTTCATCAGCAGCACCAGCAGCAGCAGCCGGCGTCGTTCATGGAGTACGGCGGCTATGGCGGCGGCTACGGCGACGAGAGCAAGCAGCAGCTGATGAACCAGGCAGCGCCGCCGCTGTCATCGATCGAGAAGTGGCTGTTCGACGAGGCGGCGGCGGAGCAGGTCGCCGACCTCATGGACCTCTCCGACGGTTGCTGCTCCGTCCCAATGATGTTTTAA
Protein Sequence
- >LOC_Os12g03150.1
MGRPPCCDKEGIKKGPWTPEEDIILVSYIQEHGPGNWRSVPINTGLMRCSKSCRLRWTNYLRPGIKRGNFTAHEEGIIVHLQSLLGNRWAAIASYLPQRTDNDIKNYWNTHLKKKLAAAANSTSSSNRHPIFADATFPSAAGGSYTVSSNSDVTQMAAIARRSPFADCPSSSYASSMDNISKLLDGFMKTNSPSPPPPPLQHYDSGYYDDVKPAVDVGGNPLLSSFDCMSGADLDCCFDVHQQHQQQQPASFMEYGGYGGGYGDESKQQLMNQAAPPLSSIEKWLFDEAAAEQVADLMDLSDGCCSVPMMF*