Gene Details:

Functional Descriptions:

  • The accumulation of the OsFAD3 mRNA in leaf tissues remained at quite low levels, both at normal growth temperatures and at chilling temperatures.
  • The ALA contents in the seeds of endoplasmic reticulum (ER)-localized GmFAD3-1 and OsFAD3 overexpression lines increased from 0.
  • Homologous OsFAD3 is more active than GmFAD3-1 in catalysing LA conversion to ALA in rice seeds.
  • Similar temperature responses of the OsFAD3 gene were observed both in chilling- tolerant and in chilling-intolerant rice cultivars.
  • In root tissues, a high level of the OsFAD3 mRNA was observed at 15 degrees C and 20 degrees C, with its level decreasing markedly at temperatures below 10 degrees C.
  • Expression of the OsFAD3 cDNA in tobacco hairy root tissues and subsequent analysis of fatty acid compositions demonstrated the activity of the microsome omega-3 fatty acid desaturase.
  • The OsFAD3 mRNA was abundant in root tissues, but was hardly detectable in leaves.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os12g01370.1
    GGGCAGAAGGCAACACAGGAGGAGGAGGAGCTTGGTTTCTGGGTTGGGTTGGGTTTGGTTGCGTTGCGGCCCATCCATCGCATTCGCGTTCGCAGGCACAAACCAAACACCTCCCCTCAAATTTCTCTTTCTTGTCTCTTCTCCCTTTCCGCCTCGCCTCGCCTCGCTGCCTGCGCTTCACAAATCACAAATCGGAATCAGATCCACCACGACACGGCGGCGGCAATGGCGGCGTCGGCGACCCAGGAGGCGGACTGCAAGGCTTCCGAGGACGCCCGTCTCTTCTTCGACGCCGCCAAGCCCCCGCCCTTCCGCATCGGCGACGTCCGCGCCGCCATCCCCGCCCACTGCTGGCGCAAGACCCCCCTCCGCTCCCTCTCCTACGTCGCCCGCGACCTCCTCATCGTCGCCGCCCTCTTCGCCGCAGCCGCCACTCGGATTGACGTCTCCGTCGCCTGGGCGGCCTGGCCGCTCTACTGGGCGGCGCAGGGCACCATGTTCTGGGCGCTCTTCGTCCTCGGCCACGACTGTGGGCACGGGAGCTTCTCCGACAGCGCCATGCTCAACAACGTCGTCGGCCACCTCCTGCACTCCTTCATCCTCGTCCCCTACCATGGATGGAGAATCAGCCACAGAACACACCATCAGAACCACGGTCATATCGAGAAGGACGAGTCCTGGCACCCGATCACCGAGAAATTGTACCGGAAGCTGGAGACGCGCACCAAGAAACTGCGCTTTACATTACCATTTCCATTGCTTGCCTTCCCTGTCTACCTCTGGTACAGAAGTCCGGGCAAGACCGGATCTCACTTTCTGCCGAGCAGCGATCTGTTCAGCCCCAAGGAGAAGAGTGACGTGATTGTCTCCACCACCTGCTGGTGCATCATGATTTCGTTGCTCGTCGCCTTGGCGTGCGTGTTTGGGTCTGTTCCGGTGCTCATGCTATACGGTGTCCCATATCTTGTATTCGTGATGTGGCTTGATTTGGTGACTTATCTGCACCACCACGGTCACAATGACCTTCCCTGGTATCGTGGAGAGGAATGGAGCTACCTCCGCGGTGGCCTGACAACGGTGGATCGGGACTACGGGTGGATCAACAACATCCACCATGACATTGGCACCCATGTCATCCACCACCTTTTCCCCCAAATTCCTCATTATCACCTCGTAGAAGCAACAAAGGCAGCAAGGCCAGTGCTGGGCAGATACTACAGGGAGCCAGAGAAGTCAGGTCCGCTGCCACTTCACCTGTTTGGCGTTCTGCTGAGGAGCTTGAGAGTTGATCACTTTGTCAGCGACGTCGGGGATGTTGTTTACTACCAAACAGATCACAGCTTGAACGGCACTGACTGGGCCGAAGATGCTAAGCACAAGTGAAAAATTAACTTGAAAATTATTGGAGTCACCGTGTACTAGTAGCTTAGTAAGTGAAGAATGGATTGTTTTCCTGATAGATAGCATATCCATCATAGCATGAACTGCTGAAAGAAAGAGAAATAATAAGCAGCAAGGGCTACTTACCTGCTCAAAAGCATGCGGCCCTGCAGAACACAGGGATTCATTGATCTGTTGGATCACCATATTCAGATCACTAAACAAGCATTGTCCAATTGTTGTTGTAGCTAGCGGGTCGTTCAGTTTTGATTAGTTCTGAAAGTGTCCAAGTTATACCGTGTAACCCTTTGGAGTTTGCCAGGCAATTCAATGTCTGGAGTTTGTATGTGCCATTGAGCAGACAATTATGATCCGAATTAAGCTCTGCACCTTAATTTTTCCATACTGAATCACTCACAATGGAGTTTATTTGC
CDS Sequence
  • >LOC_Os12g01370.1
    ATGGCGGCGTCGGCGACCCAGGAGGCGGACTGCAAGGCTTCCGAGGACGCCCGTCTCTTCTTCGACGCCGCCAAGCCCCCGCCCTTCCGCATCGGCGACGTCCGCGCCGCCATCCCCGCCCACTGCTGGCGCAAGACCCCCCTCCGCTCCCTCTCCTACGTCGCCCGCGACCTCCTCATCGTCGCCGCCCTCTTCGCCGCAGCCGCCACTCGGATTGACGTCTCCGTCGCCTGGGCGGCCTGGCCGCTCTACTGGGCGGCGCAGGGCACCATGTTCTGGGCGCTCTTCGTCCTCGGCCACGACTGTGGGCACGGGAGCTTCTCCGACAGCGCCATGCTCAACAACGTCGTCGGCCACCTCCTGCACTCCTTCATCCTCGTCCCCTACCATGGATGGAGAATCAGCCACAGAACACACCATCAGAACCACGGTCATATCGAGAAGGACGAGTCCTGGCACCCGATCACCGAGAAATTGTACCGGAAGCTGGAGACGCGCACCAAGAAACTGCGCTTTACATTACCATTTCCATTGCTTGCCTTCCCTGTCTACCTCTGGTACAGAAGTCCGGGCAAGACCGGATCTCACTTTCTGCCGAGCAGCGATCTGTTCAGCCCCAAGGAGAAGAGTGACGTGATTGTCTCCACCACCTGCTGGTGCATCATGATTTCGTTGCTCGTCGCCTTGGCGTGCGTGTTTGGGTCTGTTCCGGTGCTCATGCTATACGGTGTCCCATATCTTGTATTCGTGATGTGGCTTGATTTGGTGACTTATCTGCACCACCACGGTCACAATGACCTTCCCTGGTATCGTGGAGAGGAATGGAGCTACCTCCGCGGTGGCCTGACAACGGTGGATCGGGACTACGGGTGGATCAACAACATCCACCATGACATTGGCACCCATGTCATCCACCACCTTTTCCCCCAAATTCCTCATTATCACCTCGTAGAAGCAACAAAGGCAGCAAGGCCAGTGCTGGGCAGATACTACAGGGAGCCAGAGAAGTCAGGTCCGCTGCCACTTCACCTGTTTGGCGTTCTGCTGAGGAGCTTGAGAGTTGATCACTTTGTCAGCGACGTCGGGGATGTTGTTTACTACCAAACAGATCACAGCTTGAACGGCACTGACTGGGCCGAAGATGCTAAGCACAAGTGA
Protein Sequence
  • >LOC_Os12g01370.1
    MAASATQEADCKASEDARLFFDAAKPPPFRIGDVRAAIPAHCWRKTPLRSLSYVARDLLIVAALFAAAATRIDVSVAWAAWPLYWAAQGTMFWALFVLGHDCGHGSFSDSAMLNNVVGHLLHSFILVPYHGWRISHRTHHQNHGHIEKDESWHPITEKLYRKLETRTKKLRFTLPFPLLAFPVYLWYRSPGKTGSHFLPSSDLFSPKEKSDVIVSTTCWCIMISLLVALACVFGSVPVLMLYGVPYLVFVMWLDLVTYLHHHGHNDLPWYRGEEWSYLRGGLTTVDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATKAARPVLGRYYREPEKSGPLPLHLFGVLLRSLRVDHFVSDVGDVVYYQTDHSLNGTDWAEDAKHK*