Gene Details:
- MSU gene ID: LOC_Os11g45740
- RAPdb gene ID: Os11g0684000
- Gene Symbol: JAmyb OsJAmyb OsMYB21
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- JAmyb overexpression in transgenic Arabidopsis improved tolerance to high-salinity stress during seed germination, seedling growth, and root elongation.
- In this study, we identified a rice R2R3-type MYB transcription factor gene, JAmyb, as a gene whose overexpression causes tolerance to high salinity.
- Several transcription factors involved in the jasmonate (JA)-mediated stress response are also regulated by JAmyb.
- Role of the rice transcription factor JAmyb in abiotic stress response.
- JAmyb has been reported to be associated with disease response.
- In rice seedlings, JAmyb expression was induced by high-salinity and high-osmotic stresses and reactive oxygen species (ROS), suggesting that JAmyb is responsible for abiotic stress response.
- Microarray analysis showed that the overexpression of JAmyb stimulates the expression of several defense-associated genes, some of which have been predicted to be involved in osmotic adjustment, ROS removal, and ion homeostasis.
- Our observations suggest that JAmyb plays a role in JA-mediated abiotic stress response in addition to biotic stress response in rice.
- After inoculation with the blast isolate Hoku1, the expression of OsJAmyb was more rapidly and strongly induced in the seedlings of blast-resistant Heikezijing compared with that in the blast-susceptible landrace Suyunuo.
- The expression of OsJAmyb gene was detected in the root, stem, leaf, sheath, immature panicle and flowering panicle of rice, with highest expression in the leaf.
- The OsJAmyb overexpressing transgenic lines (T3 generation) in Suyunuo showed increased resistance to blast infection with less lesion number in inoculated leaves compared with non-transgenic control plants.
- The results indicate that OsJAmyb might be involved in the resistance to blast infection in rice.
- In this study we identified one MYB transcription factor gene OsJAmyb from rice landrace Heikezijing, which encodes a protein with 283 amino-acid residues belonging to R2R3-type MYB transcription factor family.
- Through combined analysis of differential expression and annotations of the predicted genes within qBBR11-4 between two sets of rice accessions selected based on haplotypes and disease phenotypes, we identified the transcription factor OsMYB21 as the candidate gene for qBBR11-4.
- Genome-Wide Association Mapping and Gene Expression Analysis Reveal the Negative Role of OsMYB21 in Regulating Bacterial Blight Resistance in Rice.
- The OsMYB21 overexpressing plants exhibited decreased resistance to bacterial blight, accompanied with down-regulation of several defense-related genes compared with the wild-type plants.
- Conclusion: The results suggest that OsMYB21 negatively regulates bacterial blight resistance in rice, and this gene can be a promising target in rice breeding by using the gene editing method.
Function-related keywords:
- root , transcription-factor , growth , jasmonate , disease , seedling , salinity , homeostasis , seed , seed-germination , salinity-stress , abiotic-stress , defense , seedlings , leaf , resistance , panicle , blast , lesion , blight , bacterial-blight , breeding
Literature:
- Role of the rice transcription factor JAmyb in abiotic stress response . DOI: 10.1007/s10265-012-0501-y ; PMID: 22847900
- OsJAMyb, a R2R3-type MYB transcription factor, enhanced blast resistance in transgenic rice . DOI: 10.1016/j.pmpp.2015.04.008
- Genome-Wide Association Mapping and Gene Expression Analysis Reveal the Negative Role of OsMYB21 in Regulating Bacterial Blight Resistance in Rice . DOI: 10.1186/s12284-021-00501-z ; PMID: 34185169
Related News:
Gene Resources:
- NCBI ID: AAK08983
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os11g45740.1
AGCTGCACCCTTGCATCTTCTCTCTCTCTCTCTTCTTCTTCTTCTTCTTCTCCATATCTCCTACTCCTCGTGAAGATCGATCGACCATCGGCAATTTCATTCGGTAATAGTTAAGCTAAGATCAAATCAAGATTGGCGAAACGATGGAGATGGTGCTGCAGAGGACGAGCCACCACCCGGTGCCCGGGGAGCAGCAGGAGGCGGCGGCGGAGCTGTCGTCGGCTGAGCTCCGGCGAGGGCCGTGGACCGTCGATGAGGACCTCACCCTCATCAATTACATCTCTGATCACGGCGAGGGCCGCTGGAACGCACTCGCACGCGCCGCCGGTCTGAAGAGGACTGGGAAGAGCTGCCGGCTCCGGTGGCTGAACTATCTCCGGCCGGATGTGAAGCGCGGCAACTTCACCGCAGAGGAGCAGCTGCTCATCCTCGACCTCCACTCCCGATGGGGCAACCGATGGTCCAAGATAGCACAACATTTGCCTGGGAGGACCGACAACGAGATCAAGAACTACTGGAGGACCAGAGTGCAAAAGCATGCCAAGCAACTCAATTGTGATGTCAACAGCAAGAGGTTCAAGGATGCCATGAAGTACCTATGGATGCCTCGCCTTGCCGAGCGCATCCATGCCAGGGCTGGCGCTGTTGATGATAGCGGAGACTACAGCAACAACGACTTATCATGTGTATCTGGTGTAACAATGGCCACTGTTGCTAATTGTTTTGATGGCTCTCCGAGCATGGTGACTAGCTCATCTTCCGATTCGTTCACGTCGGAGTCACAAGATTTGAAGAAGATTAACTTACATGTGCATGGTGATGATGAGAAGATGAACTCTGAAGACTGGATGCAGGAGGTGGACCATGAGTTTTGGTCTACAGAGATTCAGCCTAATAATGAACAGTTTCAGGACCAACAGCTTAACGGTTGGGTGCAAGGTTTTTCTGAGGGTTTGTCCGAAACTCTATGGAGTCTAGAGGACATATGGAAGATGCAATAAATTTTACATTTACAAAGGACATATTTGTAAAATAGAATAGAGAGACATCAAGGAAGGCAAAGAGAAAAAGTTCTTTAGTCATATACTGTACGTTGAGACACTTAAGTTCTTTGCCAACTTCAATGTTTTTTTTCTTTTATTTTTGCTGCCTCTTTCGATCCCTGAAGGGGTTTTTGCCTTCTAGATGTCTAGGGGTTCAAAGAGGACCAGAGGAGATATATATGTGTTTGGATATGTTGATATATGAGCTGATATATGCCTTACTATATATATACTCCAGAATTGCACTGAGGATTACATATCCAATTCAGACATGCAAACCTTACCAGTTGCAATATTGTAAATTACATGTCCAATTTTGTAGTTTCATCTTCCTCATCATAAAAAAATTATGTTGTGTC
CDS Sequence
- >LOC_Os11g45740.1
ATGGAGATGGTGCTGCAGAGGACGAGCCACCACCCGGTGCCCGGGGAGCAGCAGGAGGCGGCGGCGGAGCTGTCGTCGGCTGAGCTCCGGCGAGGGCCGTGGACCGTCGATGAGGACCTCACCCTCATCAATTACATCTCTGATCACGGCGAGGGCCGCTGGAACGCACTCGCACGCGCCGCCGGTCTGAAGAGGACTGGGAAGAGCTGCCGGCTCCGGTGGCTGAACTATCTCCGGCCGGATGTGAAGCGCGGCAACTTCACCGCAGAGGAGCAGCTGCTCATCCTCGACCTCCACTCCCGATGGGGCAACCGATGGTCCAAGATAGCACAACATTTGCCTGGGAGGACCGACAACGAGATCAAGAACTACTGGAGGACCAGAGTGCAAAAGCATGCCAAGCAACTCAATTGTGATGTCAACAGCAAGAGGTTCAAGGATGCCATGAAGTACCTATGGATGCCTCGCCTTGCCGAGCGCATCCATGCCAGGGCTGGCGCTGTTGATGATAGCGGAGACTACAGCAACAACGACTTATCATGTGTATCTGGTGTAACAATGGCCACTGTTGCTAATTGTTTTGATGGCTCTCCGAGCATGGTGACTAGCTCATCTTCCGATTCGTTCACGTCGGAGTCACAAGATTTGAAGAAGATTAACTTACATGTGCATGGTGATGATGAGAAGATGAACTCTGAAGACTGGATGCAGGAGGTGGACCATGAGTTTTGGTCTACAGAGATTCAGCCTAATAATGAACAGTTTCAGGACCAACAGCTTAACGGTTGGGTGCAAGGTTTTTCTGAGGGTTTGTCCGAAACTCTATGGAGTCTAGAGGACATATGGAAGATGCAATAA
Protein Sequence
- >LOC_Os11g45740.1
MEMVLQRTSHHPVPGEQQEAAAELSSAELRRGPWTVDEDLTLINYISDHGEGRWNALARAAGLKRTGKSCRLRWLNYLRPDVKRGNFTAEEQLLILDLHSRWGNRWSKIAQHLPGRTDNEIKNYWRTRVQKHAKQLNCDVNSKRFKDAMKYLWMPRLAERIHARAGAVDDSGDYSNNDLSCVSGVTMATVANCFDGSPSMVTSSSSDSFTSESQDLKKINLHVHGDDEKMNSEDWMQEVDHEFWSTEIQPNNEQFQDQQLNGWVQGFSEGLSETLWSLEDIWKMQ*