Gene Details:

Functional Descriptions:

  • The pi54 gene (Pi-k(h)) confers a high degree of resistance to diverse strains of the fungus Magnaporthe oryzae.
  • Detection of callose deposition in resistant plants containing Pi-k h (pi54) gene implicates its involvement in the initiation of defense response cascade.
  • In our effort to identify more effective forms of this gene, we isolated an orthologue of pi54 named as pi54rh from the blast-resistant wild species of rice, Oryza rhizomatis, using allele mining approach and validated by complementation.
  • The blast resistance candidate gene Pi-k h (pi54) was cloned into a plant transformation vector and the construct was used to transform a japonica cultivar of rice Taipei 309, which is susceptible to M.
  • Functional complementation of rice blast resistance gene Pi-k h (pi54) conferring resistance to diverse strains of Magnaporthe oryzae.
  • The single functional blast resistance gene pi54 activates a complex defence mechanism in rice.
  • The dominant rice blast resistance gene, pi54 confers resistance to Magnaporthe oryzae in different parts of India.
  • A novel blast resistance gene, pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae.
  • In this study, for the first time, we demonstrated that a rice blast resistance gene pi54rh cloned from wild species of rice provides broad spectrum resistance to M.
  • The pi54rh belongs to CC-NBS-LRR family of disease resistance genes with a unique Zinc finger (C(3)H type) domain.
  • This study suggests the activation of defence response and transcription factor-related genes and a higher expression of key enzymes involved in the defence response pathway in the rice line TP-pi54, thus leading to incompatible host-pathogen interaction.
  • The transgenic lines containing pi54 gene have higher tiller number, grain weight, epicotyl length, and yield compared to the non-transgenic control.
  • Phenotypic expression of blast resistance gene pi54 is not affected by its chromosomal position.
  • This is a novel report in which chromosomal position of the rice blast resistance gene pi54 was not found to affect significantly the resistance phenotype or morphological traits.
  • Three different transgenic lines containing pi54 and its orthologue pi54rh were shown to be resistant to different races of M.
  • These results will facilitate precise utilization of pi54 gene and its orthologue in breeding programs for the development of rice cultivars with broad spectrum and durable resistance to M.
  • pi54 gene imparts resistance against M.
  • Extensive sequence variation in rice blast resistance gene pi54 makes it broad spectrum in nature.
  • This is a maiden report of extensive variability of pi54 alleles in different landraces and cultivated varieties, possibly, attributing broad-spectrum resistance to Magnaporthe oryzae.
  • Rice blast resistant gene, pi54 cloned from rice line, Tetep, is effective against diverse isolates of Magnaporthe oryzae.
  • High quality sequences were generated for homologs of pi54 gene.
  • The rice blast resistance gene pi54 initially identified in an Indian cultivar confers broad-spectrum resistance in India.
  • Together, the newly identified pi54 alleles expand the allelic series and are candidates for rice blast resistance breeding programs.
  • We explored the allelic diversity of the pi54 gene among 885 Indian rice genotypes that were found resistant in our screening against field mixture of naturally existing M.
  • The objective of this study was to determine the effects (through over-expressing and using the CaMV 35S promoter) of pikh on MR219 resistance because it is a rice variety that is susceptible to the blast fungus pathotype P7.
  • This study successfully clarified that over-expression of the pikh gene in transgenic plants can improve their blast resistance against the M.
  • Over-Expression of the pikh Gene with a CaMV 35S Promoter Leads to Improved Blast Disease (Magnaporthe oryzae) Tolerance in Rice.
  • brachyantha was on the root of both pi54 and #11 alleles.
  • Divergent evolution of rice blast resistance pi54 locus in the genus Oryza.
  • The rice blast resistance gene pi54 was cloned from Oryza sativa ssp.
  • Two divergent structures of pi54 locus caused by a mobile unit containing the nearby R-gene cluster could be developed before domestication.
  • In BC3F3 generation, the improved pyramided lines carrying a total of seven genes/QTLs (xa5 + xa13 + Xa21 + pi54 + qSBR7-1 + qSBR11-1 + qSBR11-2) were selected through molecular and phenotypic assay, and these were evaluated for resistance against bacterial blight, blast, and sheath blight pathogens under greenhouse conditions.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os11g42010.1
    ATGGCAAAACTTCAAGAGAAAGAAAAGCTCGAGACATTGATGTTGAGGTGGAATATGGATGCTGGAAATGCCAGTAGAATAGATCATGAAGTACTTGAAACCCTCCAACCTAACCAGTGCCTGAAAACTTTGGAGATTGTTGCGTATGAAGGACATGCATTTCCATCTTGGATAACAAGCACTGAGCCATATCTCACATCTCTGGTTGAGATAAGCCTTGAAGTCTGGGAACAGTCATCTAGGATGAATCTGTTTCCTCGTCTTGCAGAACTTGTCATCATTCAGTGCCCAAAATTGAGAGCACTGCATATGGAGTTTCCATCGATTGAAAAATTGATTTTGTGGATGAATAATAAGATGTTGTACAGCTCGAAGGAAGGTCTGAGAGGTGTAGAGAAGAGCCTCGAAAATCTTTCAATTTCTTTCTGTGAAGAGTTGCATGCTTCTTCAGGCTGTGAGGGTCTACAGGCTCTTGATCGCCTCAAGAAGTTGGAGATATGTGGTTGTCATGAATTGTCATGCCTCCCACAGGGTTTGCAGCATCTTTCCTCTCTCGCCAGCTTGAAAATTGATAACTGCAACAAATTGGAGATTTTGCCAGAATGGTTGGAGAACCTACCTTTTCTACAGATAATGTGTTTGTCTGGCTGTCCAATTCTGCACTCCATTCCTGAAGGCCTAACATGTAGCGACATTATTGTTGAATATTGCCCCAATTTTAAAGAACCATCAGGTATGTCATCAGTGCTCTGTTCATGGAAAGCCATGTTTCTGATTTTCATTGAGCTATTCTTAAAGCAATTGAACTAG
CDS Sequence
  • >LOC_Os11g42010.1
    ATGGCAAAACTTCAAGAGAAAGAAAAGCTCGAGACATTGATGTTGAGGTGGAATATGGATGCTGGAAATGCCAGTAGAATAGATCATGAAGTACTTGAAACCCTCCAACCTAACCAGTGCCTGAAAACTTTGGAGATTGTTGCGTATGAAGGACATGCATTTCCATCTTGGATAACAAGCACTGAGCCATATCTCACATCTCTGGTTGAGATAAGCCTTGAAGTCTGGGAACAGTCATCTAGGATGAATCTGTTTCCTCGTCTTGCAGAACTTGTCATCATTCAGTGCCCAAAATTGAGAGCACTGCATATGGAGTTTCCATCGATTGAAAAATTGATTTTGTGGATGAATAATAAGATGTTGTACAGCTCGAAGGAAGGTCTGAGAGGTGTAGAGAAGAGCCTCGAAAATCTTTCAATTTCTTTCTGTGAAGAGTTGCATGCTTCTTCAGGCTGTGAGGGTCTACAGGCTCTTGATCGCCTCAAGAAGTTGGAGATATGTGGTTGTCATGAATTGTCATGCCTCCCACAGGGTTTGCAGCATCTTTCCTCTCTCGCCAGCTTGAAAATTGATAACTGCAACAAATTGGAGATTTTGCCAGAATGGTTGGAGAACCTACCTTTTCTACAGATAATGTGTTTGTCTGGCTGTCCAATTCTGCACTCCATTCCTGAAGGCCTAACATGTAGCGACATTATTGTTGAATATTGCCCCAATTTTAAAGAACCATCAGGTATGTCATCAGTGCTCTGTTCATGGAAAGCCATGTTTCTGATTTTCATTGAGCTATTCTTAAAGCAATTGAACTAG
Protein Sequence
  • >LOC_Os11g42010.1
    MAKLQEKEKLETLMLRWNMDAGNASRIDHEVLETLQPNQCLKTLEIVAYEGHAFPSWITSTEPYLTSLVEISLEVWEQSSRMNLFPRLAELVIIQCPKLRALHMEFPSIEKLILWMNNKMLYSSKEGLRGVEKSLENLSISFCEELHASSGCEGLQALDRLKKLEICGCHELSCLPQGLQHLSSLASLKIDNCNKLEILPEWLENLPFLQIMCLSGCPILHSIPEGLTCSDIIVEYCPNFKEPSGMSSVLCSWKAMFLIFIELFLKQLN*