Gene Details:

Functional Descriptions:

  • The greatly increased activities of reactive oxygen species (ROS) scavenging enzymes, and reduced photosystem (PS) II core proteins CP43, CP47 and D1 in leaves of the OsCRTISO/phs3-1mutant and OsLCY RNAi transgenic rice indicated that photo-oxidative damage occurred in PS II, consistent with the accumulation of ROS in these plants.
  • Expression analysis via both RT-PCR and ZEBRA2-promoter-beta-glucuronidase (GUS) transgenic rice indicates that ZEBRA2 is predominantly expressed in mesophyll cells of mature leaves where active photosynthesis occurs.
  • More severe phenotype of the ZEBRA2 mutant under high light intensity indicates that ““zebra”” phenotype might be caused by photooxidative damages.
  • We found that MHZ5 encodes a carotenoid isomerase and that the mutation in MHZ5 blocks carotenoid biosynthesis, reduces ABA accumulation, and promotes ethylene production in etiolated seedlings.
  • ABA can largely rescue the ethylene response of the MHZ5 mutant.
  • Ethylene induces MHZ5 expression, the production of neoxanthin, an ABA biosynthesis precursor, and ABA accumulation in roots.
  • MHZ5 overexpression results in enhanced ethylene sensitivity in roots and reduced ethylene sensitivity in coleoptiles.
  • Prolycopene was accumulated while all-trans-lycopene was barely detectable in the dark-grown MIT3 seedlings.
  • Exogenously applied GR24, a synthetic SL, could rescue the tillering phenotype of MIT3.
  • Our results reveal that the tillering phenotype of MIT3 is due to SL deficiency and directly link carotenoid deficiency with SL-regulated rice tillering.
  • Mutations in the MIT3 gene encoding a caroteniod isomerase lead to increased tiller number in rice.
  • Double mutant analysis of MIT3 with the SLs biosynthesis mutant d17 revealed that MIT3 controls tiller development upstream of the SLs biosynthesis pathway.
  • Map-based cloning revealed that MIT3 encodes a carotenoid isomerase (CRTISO), the key enzyme catalyzing the conversion from prolycopene to all-trans-lycopene in carotenoid biosynthesis.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os11g36440.1
    GCAGATTCCACCGCGGCCTCTCCTCCCATATAAAAACCCCACCCAGCTCGAGCTCCCGCGCTTCACCGGCGCCGCCATGCCGCTCCTCCTCCTCGCCGCCGCGCGCCCGTGCGCCCCGCTCCTCGCTCCCTCCGCCGCCGGAGCAGTAGTAGGCCGGCCGTCCACCGCCCGCCCACTGGGACGAGGGACCAGGAGAGGCGCGGGCGCGGCGGCGGCGGCTGCGGTGGCGGCGGAGAAGACGGTGGTGAAGGCGGAGGAGGAGGAGGAGGGTGGGGGAGAGTACGACGCGATCGTGGTGGGGTCGGGGATCGGGGGGATGGTGGCGGCGACGCAGCTGGCGGCGAAGGGGGCGAGGGTGCTGGTGCTGGAGAAGTACGTCATCCCCGGGGGGAGCTCCGGGTACTACCGCCGCGACGGGTTCACCTTCGACGTCGGCTCCTCCGTCATGTTCGGCTTCTCCGACAAGGGGAACTTGAATTTGATTACACAAGCACTAGAAGCAGTTGGGCATAAGATGGAAGTTATACCGGATCCTTCTACGGTTCATTTCCACCTACCTGGTGACCTCTCAGTTCTTGTGCATAGAGAGTATGATGACTTCGTCACGGAGCTGGTTAATAAATTTCCTCATGAAAAGGAAGGAATCCTGAAATTCTATGGCACATGTTGGAAGATCTTCAATTCATTAAATTCTTTGGAGCTGAAGTCCCTTGAGGAGCCTCTATATCTTTTTGGGCAATTTTTTCAAAAGCCTCTGGAATGTTTGACGCTAGCTTATTATCTGCCACAGAATGCTGGGGATATTGCTCGCAAGTTCATAAAAGATCAGCAGTTACTCTCCTTCATAGATGCTGAGTGCTTTATTGTGAGCACAGTCAATGCCTTGCAAACACCCATGATTAATGCAAGCATGGTCTTGTGCGATAGGCACTTCGGGGGAATTAACTATCCAGTTGGTGGTGTTGGTGGTATTGCTATGTCCTTGGCAGATGGGCTTGTTGACAAAGGCAGTGAAATACGCTACAAGGCTAATGTGACCAATGTAATTTTAGAAAATGGCAAAGCTGTTGGAGTCAGGTTATCAAATGGGAAGGAGTTCTTTGCTAAAACAGTGATATCGAATGCTACCAGATGGGATACATTTGGTAAACTCTTGAAAGTTGAAGAGCTTCCAGAAGAGGAGAAAAACTTCCAAAAGAATTATGTTAAAGCACCATCATTCCTTTCCATTCATATGGGTGTCAAAGCCTCAGTTTTGCCTGCTGATACTGATTGCCACCATTTTGTACTTGAGGATGACTGGGCTAATTTGGAGAAGCCTTATGGAAGCATATTTTTAAGTATCCCTACAGTGCTTGACCCATCCTTGGCTCCCGAAGGACACCATATACTTCATATTTTTACAACTGCAGGGATAGAGGATTGGGAAGGTCTGTCCAGGAAGGATTATGAGAAGAAAAAGGAGCTTGTGGCGACAGAGATAATAAAGAGGCTTGAAAAAAAGCTGTTCCCTGGTCTTCAGGATTCAATAGTCCTCAAGGAGGTAGGCTCACCAAAAACACACCGAAGATTTCTTGCGCGAAATGATGGTACATATGGACCCATGCCACGGGGTAAACCAAAGGGATTGCTAGCAATGCCTTTCAATACTACTTCAATAGATGGCTTGTACTGTGTTGGCGACAGCTGTTTTCCTGGGCAAGGTGTAATTGCTGTGGCATTTTCAGGGATCATGTGTGCTCATCGAGTTGCAGCTGATATTGGACTGGAACAAAGATCTCCTGTACTAGATGCGGGTCTTCTTGGGCTCCTCAGATGGTTGAGAACGCTTGCTTAGGATGATACGGACAAAGATTTGGTTGACCGTCGATTGATTTTACGATTACAGAAAAACACCTTCTCACACATGTCATGGTCATTGCACATTTGTTTTGGTGAGCCATACATGGATCAAGCAGCAGTTTTTGCCTCTTGGGATCTTGCTTCTGCCTGGTATATACAAGAGGTTGGATTTTCAGCTGCAGTGATCCCTGAAAAGCTGAAGCTTCCTGGAATAATTGTACAGATTTCAGTTGTTATTTGGGAGCAAAAGGCGTAAAGACTAATATGGATGCATATATGTACCTAGAGCCTTATACACATGATGTAACTTGTTGTAATCTACTAGTATCTCCTGTATGCCCATGTTGTCATGTTGACAATTGAGAGTTACAAACATGCAGCTGTACATGATGTGTAAGCCTTAACAGAACAAAGTCAGATACACCTGCAGCCTTCTG
CDS Sequence
  • >LOC_Os11g36440.1
    ATGCCGCTCCTCCTCCTCGCCGCCGCGCGCCCGTGCGCCCCGCTCCTCGCTCCCTCCGCCGCCGGAGCAGTAGTAGGCCGGCCGTCCACCGCCCGCCCACTGGGACGAGGGACCAGGAGAGGCGCGGGCGCGGCGGCGGCGGCTGCGGTGGCGGCGGAGAAGACGGTGGTGAAGGCGGAGGAGGAGGAGGAGGGTGGGGGAGAGTACGACGCGATCGTGGTGGGGTCGGGGATCGGGGGGATGGTGGCGGCGACGCAGCTGGCGGCGAAGGGGGCGAGGGTGCTGGTGCTGGAGAAGTACGTCATCCCCGGGGGGAGCTCCGGGTACTACCGCCGCGACGGGTTCACCTTCGACGTCGGCTCCTCCGTCATGTTCGGCTTCTCCGACAAGGGGAACTTGAATTTGATTACACAAGCACTAGAAGCAGTTGGGCATAAGATGGAAGTTATACCGGATCCTTCTACGGTTCATTTCCACCTACCTGGTGACCTCTCAGTTCTTGTGCATAGAGAGTATGATGACTTCGTCACGGAGCTGGTTAATAAATTTCCTCATGAAAAGGAAGGAATCCTGAAATTCTATGGCACATGTTGGAAGATCTTCAATTCATTAAATTCTTTGGAGCTGAAGTCCCTTGAGGAGCCTCTATATCTTTTTGGGCAATTTTTTCAAAAGCCTCTGGAATGTTTGACGCTAGCTTATTATCTGCCACAGAATGCTGGGGATATTGCTCGCAAGTTCATAAAAGATCAGCAGTTACTCTCCTTCATAGATGCTGAGTGCTTTATTGTGAGCACAGTCAATGCCTTGCAAACACCCATGATTAATGCAAGCATGGTCTTGTGCGATAGGCACTTCGGGGGAATTAACTATCCAGTTGGTGGTGTTGGTGGTATTGCTATGTCCTTGGCAGATGGGCTTGTTGACAAAGGCAGTGAAATACGCTACAAGGCTAATGTGACCAATGTAATTTTAGAAAATGGCAAAGCTGTTGGAGTCAGGTTATCAAATGGGAAGGAGTTCTTTGCTAAAACAGTGATATCGAATGCTACCAGATGGGATACATTTGGTAAACTCTTGAAAGTTGAAGAGCTTCCAGAAGAGGAGAAAAACTTCCAAAAGAATTATGTTAAAGCACCATCATTCCTTTCCATTCATATGGGTGTCAAAGCCTCAGTTTTGCCTGCTGATACTGATTGCCACCATTTTGTACTTGAGGATGACTGGGCTAATTTGGAGAAGCCTTATGGAAGCATATTTTTAAGTATCCCTACAGTGCTTGACCCATCCTTGGCTCCCGAAGGACACCATATACTTCATATTTTTACAACTGCAGGGATAGAGGATTGGGAAGGTCTGTCCAGGAAGGATTATGAGAAGAAAAAGGAGCTTGTGGCGACAGAGATAATAAAGAGGCTTGAAAAAAAGCTGTTCCCTGGTCTTCAGGATTCAATAGTCCTCAAGGAGGTAGGCTCACCAAAAACACACCGAAGATTTCTTGCGCGAAATGATGGTACATATGGACCCATGCCACGGGGTAAACCAAAGGGATTGCTAGCAATGCCTTTCAATACTACTTCAATAGATGGCTTGTACTGTGTTGGCGACAGCTGTTTTCCTGGGCAAGGTGTAATTGCTGTGGCATTTTCAGGGATCATGTGTGCTCATCGAGTTGCAGCTGATATTGGACTGGAACAAAGATCTCCTGTACTAGATGCGGGTCTTCTTGGGCTCCTCAGATGGTTGAGAACGCTTGCTTAG
Protein Sequence
  • >LOC_Os11g36440.1
    MPLLLLAAARPCAPLLAPSAAGAVVGRPSTARPLGRGTRRGAGAAAAAAVAAEKTVVKAEEEEEGGGEYDAIVVGSGIGGMVAATQLAAKGARVLVLEKYVIPGGSSGYYRRDGFTFDVGSSVMFGFSDKGNLNLITQALEAVGHKMEVIPDPSTVHFHLPGDLSVLVHREYDDFVTELVNKFPHEKEGILKFYGTCWKIFNSLNSLELKSLEEPLYLFGQFFQKPLECLTLAYYLPQNAGDIARKFIKDQQLLSFIDAECFIVSTVNALQTPMINASMVLCDRHFGGINYPVGGVGGIAMSLADGLVDKGSEIRYKANVTNVILENGKAVGVRLSNGKEFFAKTVISNATRWDTFGKLLKVEELPEEEKNFQKNYVKAPSFLSIHMGVKASVLPADTDCHHFVLEDDWANLEKPYGSIFLSIPTVLDPSLAPEGHHILHIFTTAGIEDWEGLSRKDYEKKKELVATEIIKRLEKKLFPGLQDSIVLKEVGSPKTHRRFLARNDGTYGPMPRGKPKGLLAMPFNTTSIDGLYCVGDSCFPGQGVIAVAFSGIMCAHRVAADIGLEQRSPVLDAGLLGLLRWLRTLA*