Information report for OsHSD1;LGF1
Gene Details
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Functional Descriptions
- Here we report a wax-deficient rice mutant OsHSD1 with reduced epicuticular wax crystals and thicker cuticle membrane.
- Together, our data indicated that OsHSD1 plays a specialized role in cuticle formation and lipid homeostasis, probably by mediating sterol signaling.
- Furthermore, OsHSD1 mutation resulted in faster protein degradation, but had no effect on the dehydrogenase activity.
- LGF1 regulates C30 primary alcohol synthesis, which is necessary for abundant epicuticular wax platelets, leaf hydrophobicity and gas films on submerged leaves.
- Gene function was verified by a complementation test of LGF1 expressed in the drp7 mutant background, which restored C30 primary alcohol synthesis, wax platelet abundance, leaf hydrophobicity, gas film retention, and underwater photosynthesis.
- The discovery of LGF1 provides an opportunity to better understand variation amongst rice genotypes for gas film retention ability and to target various alleles in breeding for improved submergence tolerance for yield stability in flood-prone areas.
Functional Keywords
- cuticle , R-protein , leaf , submergence , photosynthesis , tolerance , yield , breeding , submergence-tolerance
Literature and News
- OsHSD1, a hydroxysteroid dehydrogenase, is involved in cuticle formation and lipid homeostasis in rice . DOI: 10.1016/j.plantsci.2016.05.005 ; PMID: 27297988
- Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance . DOI: 10.1111/nph.15070 ; PMID: 29498045
Gene Resources
- UniProt: Q2R3W3
- EMBL: AP014967, DP000010, LC363889
- AlphaFoldDB: Q2R3W3
- EnsemblPlants: Os11t0499600-01
- Gramene: Os11t0499600-01
- KEGG: osa:4350557
- Orthologous matrix: TEPPWFR
- InterPro: IPR002347, IPR020904, IPR036291
- PANTHER: PTHR43391, PTHR43391:SF89
- SUPFAM: SSF51735
- PROSITE: PS00061, PS51257
- Gene3D: 3.40.50.720
- OrthoDB: Q2R3W3
- SWISS-MODEL: Q2R3W3
- eggNOG: KOG1205
Sequences
cDNA Sequence
- >LOC_Os11g30560.1
ATTCTCCTCACTATTTGGTGCAGCGACCCCCACCCACCCATCAATTCCTTCTTCCTTCGTCTCCTCCTCTTCTCCCTGCACACTCGTTGCTAGCTAGCTCACCCATAAATACCACGCCATTGGCCACCTCCAATCTCCAAGCTAGTTCTCACTCACACATCCCTCTCCTCCCTCCGCCGCCGCCGCCGCCGGAGCTAAGGAAGACGACGACGCATAGGTAGATAATCGTCGGCGGCCATGGCTAAGGAGGCGTTCGTGAACGGGTTCCTGAGCGTGTTCATGCACGTGGGGCTAACGCTGGTGCTGCTCGTCTACCTCCCCATCGCCTTCGCCTGCCGCGCCCTGGGCAGGCTGCTCGTCAGGCCCTTCGTCTCCGGCGAGGACCTCCGCGGCAAGGTCGTGCTCGTCACCGGCGCCTCCTCCGGCATCGGCGAGCATCTAGTGTACGAGTACGCGAAGAAGGGGGCGTGCGTGGCGCTGACGGCGAGGACGGAGATCGCCCTGCGCGCAGTGGCGAAGACGGCGCGCGACCTCGGCGCGCCGGACGTGCTCGTCGTGCCGGCGGACATCACCAAGGTCGACGACGCCAAGCGCGCCGTCGAGGAGACCGTCGCCCACTTCGGCAAATTGAATCACCTGGTGGCCAACGCAGGGATCTGGTCCAGTTGCTTCTTCGAAGAAATTACCAACATAACCGCCTTCCACAACGTGATTGATCTCAACTTCTGGGGCGCCGTGTACCCGACCTACTTCGCGTTGCCGTACCTGAAAGCCAGCCGTGGCAACATCGTCGTCACCTCCTCTGTCGCCGGCAGGGTCCCGACGGCCAGGATGAGCTTCTACAACGCGAGTAAGGGCGCGGTGATCAGGTTCTACGAAACCCTAAGGGCTGAGCTGGGCTCACATGTCCGTGTCACCATTCTCACCCCGGGCTATGTCGTCTCCAACCTCACCATGGGCAAAGGCATCCAGAAGGACGGCCATGTCGGCATTGACGAGGAGGCTCGCGACATCAATGTGGGGCCGCTGCCGGTGGGGAAGACGGAGACGCTGGCGAAGGTGGTGGTGGCGGCGGTGCGGCGGGGCGACTACTACGTGACATGGCCCGGGTGGTACTGGCCGTTCCACATGGTGATGTGCACGGCGCCGGAGCTCGTCGACTGGTTCTCCCGGACCTTCTACGTCTCCAAGTCCAGCGACCACGATGGCGACGCCCTCAGCAAGAAGATCCTCGAGGCCGTCGGCGGCAAGAAGTTCCTCTACCCCAAGACCATCCGCAGCCATGCCATCGCCGCTAGCTAATATAATGCTCATATATCACACTGATTGATCGATCCATCCAAGCTATAGTTGCAACCTACCTATATATATATAAACTTTTTTTATACATGATGATATCATGATCTTTTGATAATGATGATTGGTTTGTTTTAGCATAAGAAATACTTCAGTTAAAGAGGATTGGGAGATGAAATGGGTGTGTGGGTGGATATATAGTGTACTTGTTCTTTTACTACCCCTTTTTTTTTTGCATTTGGGCTTTTTTTTAGTTTCTTTTTTTCTTTTTTCTCTTTAAAAAATCAATGTAAGCAATGTGATGTGAATCTATTCGGATATATATGTCTACTAGTGGCCGGTGGTATGGCGCTCTGTTCTAAAACAGGGCATGAATGCAATTGATGTTCGAGCACCTGAGTTGAAACATTTATTGCACTCAAATACCCAGTGCCAGATATTTAACTATTTGCCACTCTTAT
CDS Sequence
- >LOC_Os11g30560.1
ATGGCTAAGGAGGCGTTCGTGAACGGGTTCCTGAGCGTGTTCATGCACGTGGGGCTAACGCTGGTGCTGCTCGTCTACCTCCCCATCGCCTTCGCCTGCCGCGCCCTGGGCAGGCTGCTCGTCAGGCCCTTCGTCTCCGGCGAGGACCTCCGCGGCAAGGTCGTGCTCGTCACCGGCGCCTCCTCCGGCATCGGCGAGCATCTAGTGTACGAGTACGCGAAGAAGGGGGCGTGCGTGGCGCTGACGGCGAGGACGGAGATCGCCCTGCGCGCAGTGGCGAAGACGGCGCGCGACCTCGGCGCGCCGGACGTGCTCGTCGTGCCGGCGGACATCACCAAGGTCGACGACGCCAAGCGCGCCGTCGAGGAGACCGTCGCCCACTTCGGCAAATTGAATCACCTGGTGGCCAACGCAGGGATCTGGTCCAGTTGCTTCTTCGAAGAAATTACCAACATAACCGCCTTCCACAACGTGATTGATCTCAACTTCTGGGGCGCCGTGTACCCGACCTACTTCGCGTTGCCGTACCTGAAAGCCAGCCGTGGCAACATCGTCGTCACCTCCTCTGTCGCCGGCAGGGTCCCGACGGCCAGGATGAGCTTCTACAACGCGAGTAAGGGCGCGGTGATCAGGTTCTACGAAACCCTAAGGGCTGAGCTGGGCTCACATGTCCGTGTCACCATTCTCACCCCGGGCTATGTCGTCTCCAACCTCACCATGGGCAAAGGCATCCAGAAGGACGGCCATGTCGGCATTGACGAGGAGGCTCGCGACATCAATGTGGGGCCGCTGCCGGTGGGGAAGACGGAGACGCTGGCGAAGGTGGTGGTGGCGGCGGTGCGGCGGGGCGACTACTACGTGACATGGCCCGGGTGGTACTGGCCGTTCCACATGGTGATGTGCACGGCGCCGGAGCTCGTCGACTGGTTCTCCCGGACCTTCTACGTCTCCAAGTCCAGCGACCACGATGGCGACGCCCTCAGCAAGAAGATCCTCGAGGCCGTCGGCGGCAAGAAGTTCCTCTACCCCAAGACCATCCGCAGCCATGCCATCGCCGCTAGCTAA
Protein Sequence
- >LOC_Os11g30560.1
MAKEAFVNGFLSVFMHVGLTLVLLVYLPIAFACRALGRLLVRPFVSGEDLRGKVVLVTGASSGIGEHLVYEYAKKGACVALTARTEIALRAVAKTARDLGAPDVLVVPADITKVDDAKRAVEETVAHFGKLNHLVANAGIWSSCFFEEITNITAFHNVIDLNFWGAVYPTYFALPYLKASRGNIVVTSSVAGRVPTARMSFYNASKGAVIRFYETLRAELGSHVRVTILTPGYVVSNLTMGKGIQKDGHVGIDEEARDINVGPLPVGKTETLAKVVVAAVRRGDYYVTWPGWYWPFHMVMCTAPELVDWFSRTFYVSKSSDHDGDALSKKILEAVGGKKFLYPKTIRSHAIAAS*