Information report for OsWRKY72
Gene Details
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Functional Descriptions
- In this study, we report that 35S-OsWRKY72 transgenic Arabidopsis, whose seed germination was retarded under normal conditions, emerged more sensitive to mannitol, NaCl, ABA stresses and sugar starvation than vector plants.
- In addition, northern blot analysis indicated that, in rice, OsWRKY72 was inducible by polyethylene glycol (PEG), NaCl, naphthalene acetic acid (NAA), ABA and 42 degrees C, similar to its orthologue AtWRKY75 in Arabidopsis, implying that these two WRKY genes might be required for multiple physiological processes in their plants.
- Together, these results suggest that OsWRKY72 interferes in the signal cross-talk between the ABA signal and auxin transport pathway in transgenic Arabidopsis.
- Meanwhile, 35S-OsWRKY72 transgenic Arabidopsis displayed early flowering, reduced apical dominance, lost high temperature-induced hypocotyl elongation response, and enhanced gravitropism response, which were similar to the auxin-related gene mutants aux1, axr1 and bud1.
- Further, semi-quantitative RT-PCR showed that the expression patterns of three auxin-related genes AUX1, AXR1 and BUD1 were significantly altered in rosette leaves and infl orescences of 35S-OsWRKY72 plants compared with control Arabidopsis, and two ABA-related genes ABA2 and ABI4 were induced in 35S-OsWRKY72 seedlings.
- Overexpression of OsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis.
- Using a transient expression system, we have demonstrated that OsWRKY24 and -45 repress ABA induction of the HVA22 promoter-beta-glucuronidase construct, while OsWRKY72 and -77 synergistically interact with ABA to activate this reporter construct.
- Evidences demonstrate ROS induce senescence in SSPs, and transcription factors OsWRKY72 may mediate the ROS-induced senescence.
Functional Keywords
- ABA , auxin , ethylene , senescence , temperature , seed , seed-germination , seedling , flower , transcription-factor
Literature and News
- WRKY72-type transcription factors contribute to basal immunity in tomato and Arabidopsis as well as gene-for-gene resistance mediated by the tomato R gene Mi-1 . DOI: 10.1111/j.1365-313X.2010.04232.x ; PMID: 20409007
- The WRKY Gene Family in Rice (Oryza sativa) . DOI: 10.1111/j.1744-7909.2007.00504.x
- Knockdown of GDCH gene reveals reactive oxygen species-induced leaf senescence in rice . DOI: 10.1111/pce.12078 ; PMID: 23421602
- Annotations and functional analyses of the rice WRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells . DOI: 10.1104/pp.104.054312 ; PMID: 15618416
- Overexpression of OsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis . DOI: 10.1007/s12038-010-0051-1 ; PMID: 20826955
- The CC-NB-LRR OsRLR1 mediates rice disease resistance through interaction with OsWRKY19 . DOI: 10.1111/pbi.13530 ; PMID: 33368943
Gene Resources
- UniProt: Q2R432
- EMBL: AK108860, AP014967, DP000010
- AlphaFoldDB: Q2R432
- EnsemblPlants: Os11t0490900-01
- Gramene: Os11t0490900-01
- KEGG: osa:4350545
- Orthologous matrix: QMHIYSS
- InterPro: IPR003657, IPR036576, IPR044810
- PANTHER: PTHR31221, PTHR31221:SF83
- SUPFAM: SSF118290
- PROSITE: PS50811
- Gene3D: 2.20.25.80
- OrthoDB: Q2R432
- SWISS-MODEL: Q2R432
- eggNOG: ENOG502RZAJ
Homologs
- Brachypodium distachyon: Bradi4g19060.2.p, Bradi4g19060.1.p
- Hordeum vulgare: MLOC_36657.1
- Panicum virgatum: Pavir.8KG145400.1.p
- Setaria italica: Seita.8G123100.1.p
- Setaria viridis: Sevir.8G130100.1.p
- Triticum aestivum: Traes_2BS_F3097F116.1, Traes_2DS_0F2500A60.1
- Zea mays: GRMZM2G106560_P01, GRMZM2G054125_P01
Sequences
cDNA Sequence
- >LOC_Os11g29870.1
TAGCACGCACGCACAGGAAGCCGCCCTTCAATCAATTTCGATAGTCAAAGTGAAAAAAAAAGAAGAAAAGAAGAGGACAGAAAAGTATTAAGTAGAGGGTTTATTTAGCTTGCTTACTAATCCCTATATAACCCTGGTTGTAACTTGTGAAACTCTTTCAGTCTCTTCGAATTCTCAATCTCTCTCATCAGCTCGATCTATCCAACTGAGCTATTCTCGCTTATCGAGCTCGATCGATCGCTTGGTCGAGATGGAGAACTTCCCGATACTCTTTGCGACGCAGCCGACGTCGTCTTCTACCTCCAGCTCCTACCACTTCATGAGCTCCTCTTCCGGCAGCCATGACCACCGCCACCACCATGGACTACAGGCAGGCGGCAACGGCGGTGGCGGTGGTGGAAGCTTGAGCCATGGCTTGTTCATGGGGTCGTCGTCGTCGTCAATTAGGATGGAGGAGCTCTCCAACTCCAAGCAGGCCGGCGACGTCGTCGTCGACGGTGGTGCAACGAGGAGCCCACACGGCGGCGACGGAGACGGAGCTGCGGGCGACGACGGCGGCGATGCGCAGGCAGCCGCCGCCGGAGGGAGGAAGAAGGGGGAGAAGAAGGAGCGGCGGCCGCGGTTCGCGTTCCAGACGCGCAGCCAGGTCGACATCCTCGACGACGGCTACCGGTGGAGGAAGTACGGCCAGAAGGCCGTCAAGAACAACAAATTCCCAAGGAGCTACTACCGGTGCACGCACCAAGGGTGCAACGTGAAGAAGCAGGTGCAGCGGCTGTCGCGGGACGAGACGGTGGTGGTGACGACGTACGAGGGCACGCACACGCACCCCATCGAGAAATCCAACGACAACTTCGAGCACATCCTCACCCAAATGCACATCTACTCCGGCCTCACGCCTTCATCGGCTGCGCATGCCTCATCCTCCTCACCTCTCTTCCCTTCAGCTGCTGCTGCTGCTTCCCACATGTTCCAGTAGCTTTAGCAAATTTGATCATACACAACAAATTAGTTACACGTACATATATGATCAATGCCAATGCCACACTGTACATATGCAGTGCATGCATGGATCGATGGATGTTAGCAGGAAAAATTAAGAGGCTATGTTAATTTGTACATTAAACTTAGATGAGGTGCAGTGTAAGAGAAAACTAGCTAGTGAATTAATAAGCATATGCATGATCGATCGAGATTCAGTGGCAAGCAAGATTCGGTCACGCATGCAGTTCTTTTTAATTGTTTTACTTTTTTTTGTCAACTGTAGGCCAGATGTACTAGTGATTGCACAACTTTTACAAAGAAATTTATTCACAAGATCTTGCACATTTTGGTTCTA
CDS Sequence
- >LOC_Os11g29870.1
ATGGAGAACTTCCCGATACTCTTTGCGACGCAGCCGACGTCGTCTTCTACCTCCAGCTCCTACCACTTCATGAGCTCCTCTTCCGGCAGCCATGACCACCGCCACCACCATGGACTACAGGCAGGCGGCAACGGCGGTGGCGGTGGTGGAAGCTTGAGCCATGGCTTGTTCATGGGGTCGTCGTCGTCGTCAATTAGGATGGAGGAGCTCTCCAACTCCAAGCAGGCCGGCGACGTCGTCGTCGACGGTGGTGCAACGAGGAGCCCACACGGCGGCGACGGAGACGGAGCTGCGGGCGACGACGGCGGCGATGCGCAGGCAGCCGCCGCCGGAGGGAGGAAGAAGGGGGAGAAGAAGGAGCGGCGGCCGCGGTTCGCGTTCCAGACGCGCAGCCAGGTCGACATCCTCGACGACGGCTACCGGTGGAGGAAGTACGGCCAGAAGGCCGTCAAGAACAACAAATTCCCAAGGAGCTACTACCGGTGCACGCACCAAGGGTGCAACGTGAAGAAGCAGGTGCAGCGGCTGTCGCGGGACGAGACGGTGGTGGTGACGACGTACGAGGGCACGCACACGCACCCCATCGAGAAATCCAACGACAACTTCGAGCACATCCTCACCCAAATGCACATCTACTCCGGCCTCACGCCTTCATCGGCTGCGCATGCCTCATCCTCCTCACCTCTCTTCCCTTCAGCTGCTGCTGCTGCTTCCCACATGTTCCAGTAG
Protein Sequence
- >LOC_Os11g29870.1
MENFPILFATQPTSSSTSSSYHFMSSSSGSHDHRHHHGLQAGGNGGGGGGSLSHGLFMGSSSSSIRMEELSNSKQAGDVVVDGGATRSPHGGDGDGAAGDDGGDAQAAAAGGRKKGEKKERRPRFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRLSRDETVVVTTYEGTHTHPIEKSNDNFEHILTQMHIYSGLTPSSAAHASSSSPLFPSAAAAASHMFQ*