Gene Details:

Functional Descriptions:

  • In this study, we report that 35S-OsWRKY72 transgenic Arabidopsis, whose seed germination was retarded under normal conditions, emerged more sensitive to mannitol, NaCl, ABA stresses and sugar starvation than vector plants.
  • In addition, northern blot analysis indicated that, in rice, OsWRKY72 was inducible by polyethylene glycol (PEG), NaCl, naphthalene acetic acid (NAA), ABA and 42 degrees C, similar to its orthologue AtWRKY75 in Arabidopsis, implying that these two WRKY genes might be required for multiple physiological processes in their plants.
  • Together, these results suggest that OsWRKY72 interferes in the signal cross-talk between the ABA signal and auxin transport pathway in transgenic Arabidopsis.
  • Meanwhile, 35S-OsWRKY72 transgenic Arabidopsis displayed early flowering, reduced apical dominance, lost high temperature-induced hypocotyl elongation response, and enhanced gravitropism response, which were similar to the auxin-related gene mutants aux1, axr1 and bud1.
  • Further, semi-quantitative RT-PCR showed that the expression patterns of three auxin-related genes AUX1, AXR1 and BUD1 were significantly altered in rosette leaves and infl orescences of 35S-OsWRKY72 plants compared with control Arabidopsis, and two ABA-related genes ABA2 and ABI4 were induced in 35S-OsWRKY72 seedlings.
  • Overexpression of OsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis.
  • Using a transient expression system, we have demonstrated that OsWRKY24 and -45 repress ABA induction of the HVA22 promoter-beta-glucuronidase construct, while OsWRKY72 and -77 synergistically interact with ABA to activate this reporter construct.
  • Evidences demonstrate ROS induce senescence in SSPs, and transcription factors OsWRKY72 may mediate the ROS-induced senescence.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os11g29870.1
    TAGCACGCACGCACAGGAAGCCGCCCTTCAATCAATTTCGATAGTCAAAGTGAAAAAAAAAGAAGAAAAGAAGAGGACAGAAAAGTATTAAGTAGAGGGTTTATTTAGCTTGCTTACTAATCCCTATATAACCCTGGTTGTAACTTGTGAAACTCTTTCAGTCTCTTCGAATTCTCAATCTCTCTCATCAGCTCGATCTATCCAACTGAGCTATTCTCGCTTATCGAGCTCGATCGATCGCTTGGTCGAGATGGAGAACTTCCCGATACTCTTTGCGACGCAGCCGACGTCGTCTTCTACCTCCAGCTCCTACCACTTCATGAGCTCCTCTTCCGGCAGCCATGACCACCGCCACCACCATGGACTACAGGCAGGCGGCAACGGCGGTGGCGGTGGTGGAAGCTTGAGCCATGGCTTGTTCATGGGGTCGTCGTCGTCGTCAATTAGGATGGAGGAGCTCTCCAACTCCAAGCAGGCCGGCGACGTCGTCGTCGACGGTGGTGCAACGAGGAGCCCACACGGCGGCGACGGAGACGGAGCTGCGGGCGACGACGGCGGCGATGCGCAGGCAGCCGCCGCCGGAGGGAGGAAGAAGGGGGAGAAGAAGGAGCGGCGGCCGCGGTTCGCGTTCCAGACGCGCAGCCAGGTCGACATCCTCGACGACGGCTACCGGTGGAGGAAGTACGGCCAGAAGGCCGTCAAGAACAACAAATTCCCAAGGAGCTACTACCGGTGCACGCACCAAGGGTGCAACGTGAAGAAGCAGGTGCAGCGGCTGTCGCGGGACGAGACGGTGGTGGTGACGACGTACGAGGGCACGCACACGCACCCCATCGAGAAATCCAACGACAACTTCGAGCACATCCTCACCCAAATGCACATCTACTCCGGCCTCACGCCTTCATCGGCTGCGCATGCCTCATCCTCCTCACCTCTCTTCCCTTCAGCTGCTGCTGCTGCTTCCCACATGTTCCAGTAGCTTTAGCAAATTTGATCATACACAACAAATTAGTTACACGTACATATATGATCAATGCCAATGCCACACTGTACATATGCAGTGCATGCATGGATCGATGGATGTTAGCAGGAAAAATTAAGAGGCTATGTTAATTTGTACATTAAACTTAGATGAGGTGCAGTGTAAGAGAAAACTAGCTAGTGAATTAATAAGCATATGCATGATCGATCGAGATTCAGTGGCAAGCAAGATTCGGTCACGCATGCAGTTCTTTTTAATTGTTTTACTTTTTTTTGTCAACTGTAGGCCAGATGTACTAGTGATTGCACAACTTTTACAAAGAAATTTATTCACAAGATCTTGCACATTTTGGTTCTA
CDS Sequence
  • >LOC_Os11g29870.1
    ATGGAGAACTTCCCGATACTCTTTGCGACGCAGCCGACGTCGTCTTCTACCTCCAGCTCCTACCACTTCATGAGCTCCTCTTCCGGCAGCCATGACCACCGCCACCACCATGGACTACAGGCAGGCGGCAACGGCGGTGGCGGTGGTGGAAGCTTGAGCCATGGCTTGTTCATGGGGTCGTCGTCGTCGTCAATTAGGATGGAGGAGCTCTCCAACTCCAAGCAGGCCGGCGACGTCGTCGTCGACGGTGGTGCAACGAGGAGCCCACACGGCGGCGACGGAGACGGAGCTGCGGGCGACGACGGCGGCGATGCGCAGGCAGCCGCCGCCGGAGGGAGGAAGAAGGGGGAGAAGAAGGAGCGGCGGCCGCGGTTCGCGTTCCAGACGCGCAGCCAGGTCGACATCCTCGACGACGGCTACCGGTGGAGGAAGTACGGCCAGAAGGCCGTCAAGAACAACAAATTCCCAAGGAGCTACTACCGGTGCACGCACCAAGGGTGCAACGTGAAGAAGCAGGTGCAGCGGCTGTCGCGGGACGAGACGGTGGTGGTGACGACGTACGAGGGCACGCACACGCACCCCATCGAGAAATCCAACGACAACTTCGAGCACATCCTCACCCAAATGCACATCTACTCCGGCCTCACGCCTTCATCGGCTGCGCATGCCTCATCCTCCTCACCTCTCTTCCCTTCAGCTGCTGCTGCTGCTTCCCACATGTTCCAGTAG
Protein Sequence
  • >LOC_Os11g29870.1
    MENFPILFATQPTSSSTSSSYHFMSSSSGSHDHRHHHGLQAGGNGGGGGGSLSHGLFMGSSSSSIRMEELSNSKQAGDVVVDGGATRSPHGGDGDGAAGDDGGDAQAAAAGGRKKGEKKERRPRFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRLSRDETVVVTTYEGTHTHPIEKSNDNFEHILTQMHIYSGLTPSSAAHASSSSPLFPSAAAAASHMFQ*