Gene Details:
- MSU gene ID: LOC_Os11g28300
- RAPdb gene ID: Os11g0472500
- Gene Symbol: OsPML3
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- OsPML3 was highly expressed in rapidly developing tissues such as young leaves, root caps, lateral root primordia, and young anthers.
- At the vegetative stage, we observed necrotic root tips and lateral root primordia, and chlorotic young leaves in OsPML3 knockout lines under Mn-deficient conditions.
- Heterologous expression of OsPML3 restored the growth of Mn uptake-defective yeast strain Δsmf1 under Mn-limited conditions.
- OsPML3 knockout lines grown in the paddy field had reduced pollen fertility.
- The Golgi-localized transporter OsPML3 is involved in manganese homeostasis and complex N-glycan synthesis in rice.
- Collectively, our results indicate that OsPML3 maintains Mn homeostasis in the Golgi apparatus of the rapidly developing rice tissues, and regulates the deposition of cell wall polysaccharides and late-stage Golgi N-glycosylation, especially biosynthesis of the Lewis A structure.
- Additionally, knocking out OsPML3 reduced the deposition of cell wall polysaccharides and the content of Lea (Lewis A structure)-containing N-glycan in roots and young leaves.
Function-related keywords:
- root , growth , fertility , pollen , vegetative , homeostasis , transporter , cell-wall , manganese , lateral-root , Pollen-Fertility
Literature:
- The Golgi-localized transporter OsPML3 is involved in manganese homeostasis and complex N-glycan synthesis in rice . DOI: 10.1093/jxb/erad004 ; PMID: 36637130
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os11g28300.1
GTTAATCCCTCGATCTAAAGAAAAAAAAAACTCGTTAATCCCTCACGCGGCTTCTCAAATTCGCACGCGACGGGATAAAAAGAAAAAAGAAGAGGAATAATTCGAAGGGAAGAGGAGGAGGAGGATCTCAATATCTCATCCTGCTGCCACTGCTCGACTCCTCCTACCCTCCCCCAAATCCGCGAGCCTCTAGGGTTCGTGGGATCCCCCGGAAATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGATTAACAGCATTAACAGCTCCTTACTCTTTCTTGATACCCTTTGGTCACCCTGCTCCCTGTGTTGAGCAAATGAGTAGAGGAATTAAATTGATTCTTTGGGCACCTCTACGCTACGATTCGCAATTCATGTATAGCCCTTGCAAATGAAATTGTTCCTGACCATGCTACTTCACTTCAGTCTATAGCAGAAGCCGTCTCGATGATGAAATCATGTCTGATTTTTTGCTTGGTTGTACCTGACATCTAACTTATACAATATATATACACTATGTTGTTCAAGGTTCCTTGCGTATAGAATATTATTCTGGTACCATTTTCTATCTACTGTTGTCCATTTTCTTTCAGCCATACAAAGTTTAGGATGAAAAAGAGGTGAGAATAAATCAAACCATAAATTCACCTTTTGGAACTTAATAATCTGGACCTGCTACAGCTTTGCACAGTTGTACCCCAGGCTTATACCAGGACATTTTGTATGGATTTAATGTTGATTGAATGGCACATTTATCTTATTTGAGTTATGATCACCGCAAGTTCTCATATGCATATATTTGTACC - >LOC_Os11g28300.3
GTTAATCCCTCGATCTAAAGAAAAAAAAAACTCGTTAATCCCTCACGCGGCTTCTCAAATTCGCACGCGACGGGATAAAAAGAAAAAAGAAGAGGAATAATTCGAAGGGAAGAGGAGGAGGAGGATCTCAATATCTCATCCTGCTGCCACTGCTCGACTCCTCCTACCCTCCCCCAAATCCGCGAGCCTCTAGGGTTCGTGGGATCCCCCGGAAATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGATTAACAGCATTAACAGCTCCTTACTCTTTCTTGATACCCTTTGGTCACCCTGCTCCCTGTGTTGAGCAAATGAGTAGAGGAATTAAATTGATTCTTTGGGCACCTCTACGCTACGATTCGCAATTCATGTATAGCCCTTGCAAATGAAATTGTTCCTGACCATGCTACTTCACTTCAGTCTATAGCAGAAGCCGTCTCGATGATGAAATCATGTCTGATTTTTTGCTTGGTTGTACCTGACATCTAACTTATACAATATATATACACTATGTTGTTCAAGGTTCCTTGCGTATAGAATATTATTCTGGTACCATTTTCTATCTACTGTTGTCCATTTTCTTTCAGCCATACAAAGTTTAGGATGAAAAAGAGGTGAGAATAAATCAAACCATAAATTCACCTTTTGGAACTTAATAATCTGGACCTGCTACAGCTTTGCACAGTTGTACCCCAGGCTTATACCAGGACATTTTGTATGGATTTAATGTTGATTGAATGGCACATTTATCTTATTTGAGTTATGATCACCGCAAGTTCTCATATGCATATATTTGTACC - >LOC_Os11g28300.2
GTTAATCCCTCGATCTAAAGAAAAAAAAAACTCGTTAATCCCTCACGCGGCTTCTCAAATTCGCACGCGACGGGATAAAAAGAAAAAAGAAGAGGAATAATTCGAAGGGAAGAGGAGGAGGAGGATCTCAATATCTCATCCTGCTGCCACTGCTCGACTCCTCCTACCCTCCCCCAAATCCGCGAGCCTCTAGGGTTCGTGGGATCCCCCGGAAATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGATTAACAGCATTAACAGCTCCTTACTCTTTCTTGATACCCTTTGGTCACCCTGCTCCCTGTGTTGAGCAAATGAGTAGAGGAATTAAATTGATTCTTTGGGCACCTCTACGCTACGATTCGCAATTCATGTATAGCCCTTGCAAATGAAATTGTTCCTGACCATGCTACTTCACTTCAGTCTATAGCAGAAGCCGTCTCGATGATGAAATCATGTCTGATTTTTTGCTTGGTTGTACCTGACATCTAACTTATACAATATATATACACTATGTTGTTCAAGGTTCCTTGCGTATAGAATATTATTCTGGTACCATTTTCTATCTACTGTTGTCCATTTTCTTTCAGCCATACAAAGTTTAGGATGAAAAAGAGGTGAGAATAAATCAAACCATAAATTCACCTTTTGGAACTTAATAATCTGGACCTGCTACAGCTTTGCACAGTTGTACCCCAGGCTTATACCAGGACATTTTGTATGGATTTAATGTTGATTGAATGGCACATTTATCTTATTTGAGTTATGATCACCGCAAGTTCTCATATGCATATATTTGTACC
CDS Sequence
- >LOC_Os11g28300.1
ATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGA - >LOC_Os11g28300.3
ATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGA - >LOC_Os11g28300.2
ATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGA
Protein Sequence
- >LOC_Os11g28300.1
MDPNPRLLILLVLLAFSATVAVAEDGESTGGSKVSLGRRAGGFLHGLKKEAVVEGDHGVALDEVGPGLFDALFASLSMILVSEIGDETFIIAALMAMRHPKSIVLSGALSALYVMTVLSTGLGRIVPNLISRKHTNSAATVLYLFFGLRLLYIAWKSDPKGSQKKEMEEVEEKLESGQGKSTLRRFFGRFCTPIFLEAFILTFLAEWGDRSQIATIALATHKNAIGVAVGASLGHTVCTSLAVIGGSMLASKISQRTVATIGGVLFLGFSVSSYFYPPL* - >LOC_Os11g28300.3
MDPNPRLLILLVLLAFSATVAVAEDGESTGGSKVSLGRRAGGFLHGLKKEAVVEGDHGVALDEVGPGLFDALFASLSMILVSEIGDETFIIAALMAMRHPKSIVLSGALSALYVMTVLSTGLGRIVPNLISRKHTNSAATVLYLFFGLRLLYIAWKSDPKGSQKKEMEEVEEKLESGQGKSTLRRFFGRFCTPIFLEAFILTFLAEWGDRSQIATIALATHKNAIGVAVGASLGHTVCTSLAVIGGSMLASKISQRTVATIGGVLFLGFSVSSYFYPPL* - >LOC_Os11g28300.2
MDPNPRLLILLVLLAFSATVAVAEDGESTGGSKVSLGRRAGGFLHGLKKEAVVEGDHGVALDEVGPGLFDALFASLSMILVSEIGDETFIIAALMAMRHPKSIVLSGALSALYVMTVLSTGLGRIVPNLISRKHTNSAATVLYLFFGLRLLYIAWKSDPKGSQKKEMEEVEEKLESGQGKSTLRRFFGRFCTPIFLEAFILTFLAEWGDRSQIATIALATHKNAIGVAVGASLGHTVCTSLAVIGGSMLASKISQRTVATIGGVLFLGFSVSSYFYPPL*