Gene Details:

Functional Descriptions:

  • OsPML3 was highly expressed in rapidly developing tissues such as young leaves, root caps, lateral root primordia, and young anthers.
  • At the vegetative stage, we observed necrotic root tips and lateral root primordia, and chlorotic young leaves in OsPML3 knockout lines under Mn-deficient conditions.
  • Heterologous expression of OsPML3 restored the growth of Mn uptake-defective yeast strain Δsmf1 under Mn-limited conditions.
  • OsPML3 knockout lines grown in the paddy field had reduced pollen fertility.
  • The Golgi-localized transporter OsPML3 is involved in manganese homeostasis and complex N-glycan synthesis in rice.
  • Collectively, our results indicate that OsPML3 maintains Mn homeostasis in the Golgi apparatus of the rapidly developing rice tissues, and regulates the deposition of cell wall polysaccharides and late-stage Golgi N-glycosylation, especially biosynthesis of the Lewis A structure.
  • Additionally, knocking out OsPML3 reduced the deposition of cell wall polysaccharides and the content of Lea (Lewis A structure)-containing N-glycan in roots and young leaves.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os11g28300.1
    GTTAATCCCTCGATCTAAAGAAAAAAAAAACTCGTTAATCCCTCACGCGGCTTCTCAAATTCGCACGCGACGGGATAAAAAGAAAAAAGAAGAGGAATAATTCGAAGGGAAGAGGAGGAGGAGGATCTCAATATCTCATCCTGCTGCCACTGCTCGACTCCTCCTACCCTCCCCCAAATCCGCGAGCCTCTAGGGTTCGTGGGATCCCCCGGAAATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGATTAACAGCATTAACAGCTCCTTACTCTTTCTTGATACCCTTTGGTCACCCTGCTCCCTGTGTTGAGCAAATGAGTAGAGGAATTAAATTGATTCTTTGGGCACCTCTACGCTACGATTCGCAATTCATGTATAGCCCTTGCAAATGAAATTGTTCCTGACCATGCTACTTCACTTCAGTCTATAGCAGAAGCCGTCTCGATGATGAAATCATGTCTGATTTTTTGCTTGGTTGTACCTGACATCTAACTTATACAATATATATACACTATGTTGTTCAAGGTTCCTTGCGTATAGAATATTATTCTGGTACCATTTTCTATCTACTGTTGTCCATTTTCTTTCAGCCATACAAAGTTTAGGATGAAAAAGAGGTGAGAATAAATCAAACCATAAATTCACCTTTTGGAACTTAATAATCTGGACCTGCTACAGCTTTGCACAGTTGTACCCCAGGCTTATACCAGGACATTTTGTATGGATTTAATGTTGATTGAATGGCACATTTATCTTATTTGAGTTATGATCACCGCAAGTTCTCATATGCATATATTTGTACC
  • >LOC_Os11g28300.3
    GTTAATCCCTCGATCTAAAGAAAAAAAAAACTCGTTAATCCCTCACGCGGCTTCTCAAATTCGCACGCGACGGGATAAAAAGAAAAAAGAAGAGGAATAATTCGAAGGGAAGAGGAGGAGGAGGATCTCAATATCTCATCCTGCTGCCACTGCTCGACTCCTCCTACCCTCCCCCAAATCCGCGAGCCTCTAGGGTTCGTGGGATCCCCCGGAAATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGATTAACAGCATTAACAGCTCCTTACTCTTTCTTGATACCCTTTGGTCACCCTGCTCCCTGTGTTGAGCAAATGAGTAGAGGAATTAAATTGATTCTTTGGGCACCTCTACGCTACGATTCGCAATTCATGTATAGCCCTTGCAAATGAAATTGTTCCTGACCATGCTACTTCACTTCAGTCTATAGCAGAAGCCGTCTCGATGATGAAATCATGTCTGATTTTTTGCTTGGTTGTACCTGACATCTAACTTATACAATATATATACACTATGTTGTTCAAGGTTCCTTGCGTATAGAATATTATTCTGGTACCATTTTCTATCTACTGTTGTCCATTTTCTTTCAGCCATACAAAGTTTAGGATGAAAAAGAGGTGAGAATAAATCAAACCATAAATTCACCTTTTGGAACTTAATAATCTGGACCTGCTACAGCTTTGCACAGTTGTACCCCAGGCTTATACCAGGACATTTTGTATGGATTTAATGTTGATTGAATGGCACATTTATCTTATTTGAGTTATGATCACCGCAAGTTCTCATATGCATATATTTGTACC
  • >LOC_Os11g28300.2
    GTTAATCCCTCGATCTAAAGAAAAAAAAAACTCGTTAATCCCTCACGCGGCTTCTCAAATTCGCACGCGACGGGATAAAAAGAAAAAAGAAGAGGAATAATTCGAAGGGAAGAGGAGGAGGAGGATCTCAATATCTCATCCTGCTGCCACTGCTCGACTCCTCCTACCCTCCCCCAAATCCGCGAGCCTCTAGGGTTCGTGGGATCCCCCGGAAATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGATTAACAGCATTAACAGCTCCTTACTCTTTCTTGATACCCTTTGGTCACCCTGCTCCCTGTGTTGAGCAAATGAGTAGAGGAATTAAATTGATTCTTTGGGCACCTCTACGCTACGATTCGCAATTCATGTATAGCCCTTGCAAATGAAATTGTTCCTGACCATGCTACTTCACTTCAGTCTATAGCAGAAGCCGTCTCGATGATGAAATCATGTCTGATTTTTTGCTTGGTTGTACCTGACATCTAACTTATACAATATATATACACTATGTTGTTCAAGGTTCCTTGCGTATAGAATATTATTCTGGTACCATTTTCTATCTACTGTTGTCCATTTTCTTTCAGCCATACAAAGTTTAGGATGAAAAAGAGGTGAGAATAAATCAAACCATAAATTCACCTTTTGGAACTTAATAATCTGGACCTGCTACAGCTTTGCACAGTTGTACCCCAGGCTTATACCAGGACATTTTGTATGGATTTAATGTTGATTGAATGGCACATTTATCTTATTTGAGTTATGATCACCGCAAGTTCTCATATGCATATATTTGTACC
CDS Sequence
  • >LOC_Os11g28300.1
    ATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGA
  • >LOC_Os11g28300.3
    ATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGA
  • >LOC_Os11g28300.2
    ATGGATCCGAACCCTAGACTTCTCATCCTACTCGTCCTCCTCGCGTTCTCCGCCACCGTGGCGGTCGCCGAGGACGGCGAATCGACGGGGGGATCGAAGGTCTCGCTGGGGCGCCGTGCAGGGGGGTTCTTGCACGGGCTGAAGAAGGAAGCAGTGGTGGAGGGAGACCATGGGGTGGCCCTCGATGAGGTCGGCCCCGGGCTGTTCGATGCGCTCTTCGCGAGTCTCTCGATGATCTTAGTCAGCGAGATTGGAGATGAAACCTTTATTATTGCGGCGCTAATGGCAATGCGGCACCCCAAGTCAATCGTTCTGTCTGGTGCGCTATCGGCGCTCTACGTGATGACGGTACTATCAACTGGACTTGGTAGGATAGTCCCCAACTTGATATCAAGGAAGCACACTAACAGTGCTGCTACAGTTTTGTACTTGTTCTTTGGACTACGTTTGTTATACATTGCTTGGAAGTCTGACCCAAAGGGATCTCAGAAGAAGGAAATGGAAGAAGTGGAAGAGAAGCTTGAGTCAGGTCAAGGAAAATCAACCCTTCGCCGGTTCTTTGGAAGATTTTGTACACCGATCTTTTTGGAGGCTTTTATCTTAACCTTCTTAGCTGAATGGGGCGACCGAAGCCAAATAGCAACTATTGCGCTTGCAACCCACAAGAATGCAATCGGAGTAGCAGTTGGAGCATCATTGGGCCACACTGTCTGCACATCACTTGCAGTCATAGGTGGGAGCATGTTGGCATCCAAGATTTCACAACGGACAGTTGCAACAATCGGAGGTGTTCTCTTCTTGGGGTTCTCCGTGTCATCGTACTTCTATCCCCCACTATGA
Protein Sequence
  • >LOC_Os11g28300.1
    MDPNPRLLILLVLLAFSATVAVAEDGESTGGSKVSLGRRAGGFLHGLKKEAVVEGDHGVALDEVGPGLFDALFASLSMILVSEIGDETFIIAALMAMRHPKSIVLSGALSALYVMTVLSTGLGRIVPNLISRKHTNSAATVLYLFFGLRLLYIAWKSDPKGSQKKEMEEVEEKLESGQGKSTLRRFFGRFCTPIFLEAFILTFLAEWGDRSQIATIALATHKNAIGVAVGASLGHTVCTSLAVIGGSMLASKISQRTVATIGGVLFLGFSVSSYFYPPL*
  • >LOC_Os11g28300.3
    MDPNPRLLILLVLLAFSATVAVAEDGESTGGSKVSLGRRAGGFLHGLKKEAVVEGDHGVALDEVGPGLFDALFASLSMILVSEIGDETFIIAALMAMRHPKSIVLSGALSALYVMTVLSTGLGRIVPNLISRKHTNSAATVLYLFFGLRLLYIAWKSDPKGSQKKEMEEVEEKLESGQGKSTLRRFFGRFCTPIFLEAFILTFLAEWGDRSQIATIALATHKNAIGVAVGASLGHTVCTSLAVIGGSMLASKISQRTVATIGGVLFLGFSVSSYFYPPL*
  • >LOC_Os11g28300.2
    MDPNPRLLILLVLLAFSATVAVAEDGESTGGSKVSLGRRAGGFLHGLKKEAVVEGDHGVALDEVGPGLFDALFASLSMILVSEIGDETFIIAALMAMRHPKSIVLSGALSALYVMTVLSTGLGRIVPNLISRKHTNSAATVLYLFFGLRLLYIAWKSDPKGSQKKEMEEVEEKLESGQGKSTLRRFFGRFCTPIFLEAFILTFLAEWGDRSQIATIALATHKNAIGVAVGASLGHTVCTSLAVIGGSMLASKISQRTVATIGGVLFLGFSVSSYFYPPL*