Information report for BBM1;OsASGR-BBML1
Gene Details
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Functional Descriptions
- When genome editing to substitute mitosis for meiosis (MiMe)3,4 is combined with the expression of BBM1 in the egg cell, clonal progeny can be obtained that retain genome-wide parental heterozygosity.
- In addition, the suppression of the function of OsASGR-BBML1 and its homologues in zygotes resulted in the developmental arrest, suggesting that OsASGR-BBML1 possesses an important role in initiating zygotic development.
- Interestingly, ectopic expression of OsASGR-BBML1 in egg cells induced nuclear and cell divisions, indicating that exogenously expressed OsASGR-BBML1 converts the proliferation status of the egg cell from quiescent to active.
- We identify OsYUCCA (OsYUC) auxin biosynthesis genes as direct targets of OsBBM1.
- In somatic embryogenesis, exogenous auxin triggers OsBBM1 expression, which then activates endogenous auxin biosynthesis OsYUC genes.
- We used gene editing, transcriptome profiling, and chromatin immunoprecipitation to determine the molecular players involved in embryo initiation downstream of OsBBM1.
- Zygotic embryo initiation involves a partnership of male and female genomes, through which paternal OsBBM1 activates maternal OsYUC genes.
- Ectopic OsBBM1 initiates somatic embryogenesis without exogenous auxins but requires functional OsYUC genes.
Functional Keywords
Literature and News
- A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds . DOI: 10.1038/s41586-018-0785-8 ; PMID: 30542157
- Expression of Genes from Paternal Alleles in Rice Zygotes and Involvement of OsASGR-BBML1 in Initiation of Zygotic Development . DOI: 10.1093/pcp/pcz030 ; PMID: 30801122
- Effect of Paternal Genome Excess on the Developmental and Gene Expression Profiles of Polyspermic Zygotes in Rice . DOI: 10.3390/plants10020255 ; PMID: 33525652
- Somatic embryo initiation by rice BABY BOOM1 involves activation of zygote-expressed auxin biosynthesis genes . DOI: 10.1111/nph.18774 ; PMID: 36707918
Sequences
cDNA Sequence
- >LOC_Os11g19060.1
CTCTCTCTCTCTCTCACTGTCCACATCTCTCCAACAAGAGAAATACTTTACACACTTGCAGCAACTCTTCCCCAGATGGCCTCCATCACCAACTGGCTCGGCTTCTCCTCCTCCTCCTTCTCCGGCGCCGGCGCCGACCCCGTCCTGCCCCACCCGCCGCTGCAAGAGTGGGGGAGCGCTTATGAGGGCGGCGGCACGGTGGCGGCCGCCGGCGGGGAGGAGACGGCGGCGCCGAAGCTGGAGGACTTCCTCGGCATGCAGGTGCAGCAGGAGACGGCCGCCGCGGCGGCGGGGCACGGCCGTGGAGGCAGCTCGTCGGTCGTTGGGCTGTCCATGATCAAGAACTGGCTACGCAGCCAGCCGCCGCCCGCGGTGGTTGGGGGAGAAGACGCTATGATGGCGCTCGCGGTGTCGACGTCGGCGTCGCCGCCGGTGGACGCGACGGTGCCGGCCTGCATTTCGCCGGATGGGATGGGGTCGAAGGCGGCCGACGGCGGCGGCGCGGCCGAGGCGGCGGCGGCGGCGGCGGCGCAGAGGATGAAGGCGGCCATGGACACGTTCGGGCAGCGGACGTCCATCTACCGGGGTGTCACCAAGCACAGGTGGACAGGAAGGTATGAAGCCCATCTTTGGGATAACAGCTGCAGAAGAGAAGGTCAGACTCGCAAAGGCAGACAAGTCAATGCAGGAGGATATGATAAGGAAGAAAAAGCTGCTAGGGCTTATGATTTGGCTGCCCTTAAATACTGGGGCACTACAACGACGACGAATTTTCCGGTAAGCAACTACGAAAAAGAGTTGGATGAAATGAAGCACATGAATAGGCAGGAATTTGTTGCATCCCTTAGAAGAAAAAGCAGTGGATTTTCACGTGGTGCTTCCATATATCGTGGTGTTACAAGACACCATCAGCATGGAAGGTGGCAAGCAAGGATAGGACGGGTGGCAGGAAACAAGGATCTGTATTTGGGCACATTTGGCACCCAAGAGGAAGCTGCAGAGGCATATGATATCGCTGCAATCAAATTCCGTGGTCTCAATGCTGTGACAAACTTTGACATGAGCCGGTACGATGTCAAGAGCATCATTGAAAGCAGCAATCTCCCAATTGGTACTGGAACCACCCGGCGATTGAAGGACTCCTCTGATCACACTGATAATGTCATGGACATCAATGTCAATACCGAACCCAATAATGTGGTATCATCCCACTTCACCAATGGGGTTGGCAACTATGGTTCGCAGCATTATGGTTACAATGGATGGTCGCCAATTAGCATGCAGCCGATCCCCTCGCAGTACGCCAACGGCCAGCCCAGGGCATGGTTGAAACAAGAGCAGGACAGCTCTGTGGTTACAGCGGCGCAGAACCTGCACAATCTACATCATTTTAGTTCCTTGGGCTACACCCACAACTTCTTCCAGCAATCTGATGTTCCAGACGTCACAGGTTTCGTTGATGCGCCTTCGAGGTCCAGTGACTCATACTCCTTCAGGTACAATGGAACAAATGGCTTTCATGGTCTCCCGGGTGGAATCAGCTATGCTATGCCGGTTGCGACAGCGGTGGACCAAGGTCAGGGCATCCATGGCTATGGAGAAGATGGTGTGGCAGGCATTGACACCACACATGACCTGTATGGCAGCCGTAATGTGTACTACCTTTCCGAGGGTTCGCTTCTTGCCGATGTCGAAAAAGAAGGCGACTATGGCCAATCTGTGGGGGGCAACAGCTGGGTTTTGCCGACACCGTAGTCAGAGATGGTGGAGAAGGCTGACAATGTGACGGTTTGCCATGGGATGCCTCTGTTCTCAGTCTGGAATGATGCTTAGCTGGGTGGAGAATTAGCTATAGTAGTTACTGAAAAGGTGATATTAACAGTTGTTGGGCAGATTGATTAACTGGGGGATATATAGTAGTCATACAAAGTCTTGCATGCTTGCTGATTTTGTATTGCTTTCTTATCACCACCATGCATGCAATCTATAGTTTTGCCTTTAGCTAGAAATATTAGCTCTAAATATCTCATGGAGATATGAATTTTATGCTGATGTTACTTAGGGCTCTTGATTATATATATGAGCTGATAATTCAGCCATTATCATCTCTTGCTTTCTGAGCTAGGTGATGTATAAATTTTCGTATGGAAGAGGTTTATGTTGCAGTACTGTTGATAATTCAGTCAGCAGGTTAAATTAAAGCTAAGTGTAACTATTGTGCTGGATCGACTTTGTTTCTTTTTCAAGATTTTACAATGTTGCACTTCGCCTGATGA
CDS Sequence
- >LOC_Os11g19060.1
ATGGCCTCCATCACCAACTGGCTCGGCTTCTCCTCCTCCTCCTTCTCCGGCGCCGGCGCCGACCCCGTCCTGCCCCACCCGCCGCTGCAAGAGTGGGGGAGCGCTTATGAGGGCGGCGGCACGGTGGCGGCCGCCGGCGGGGAGGAGACGGCGGCGCCGAAGCTGGAGGACTTCCTCGGCATGCAGGTGCAGCAGGAGACGGCCGCCGCGGCGGCGGGGCACGGCCGTGGAGGCAGCTCGTCGGTCGTTGGGCTGTCCATGATCAAGAACTGGCTACGCAGCCAGCCGCCGCCCGCGGTGGTTGGGGGAGAAGACGCTATGATGGCGCTCGCGGTGTCGACGTCGGCGTCGCCGCCGGTGGACGCGACGGTGCCGGCCTGCATTTCGCCGGATGGGATGGGGTCGAAGGCGGCCGACGGCGGCGGCGCGGCCGAGGCGGCGGCGGCGGCGGCGGCGCAGAGGATGAAGGCGGCCATGGACACGTTCGGGCAGCGGACGTCCATCTACCGGGGTGTCACCAAGCACAGGTGGACAGGAAGGTATGAAGCCCATCTTTGGGATAACAGCTGCAGAAGAGAAGGTCAGACTCGCAAAGGCAGACAAGTCAATGCAGGAGGATATGATAAGGAAGAAAAAGCTGCTAGGGCTTATGATTTGGCTGCCCTTAAATACTGGGGCACTACAACGACGACGAATTTTCCGGTAAGCAACTACGAAAAAGAGTTGGATGAAATGAAGCACATGAATAGGCAGGAATTTGTTGCATCCCTTAGAAGAAAAAGCAGTGGATTTTCACGTGGTGCTTCCATATATCGTGGTGTTACAAGACACCATCAGCATGGAAGGTGGCAAGCAAGGATAGGACGGGTGGCAGGAAACAAGGATCTGTATTTGGGCACATTTGGCACCCAAGAGGAAGCTGCAGAGGCATATGATATCGCTGCAATCAAATTCCGTGGTCTCAATGCTGTGACAAACTTTGACATGAGCCGGTACGATGTCAAGAGCATCATTGAAAGCAGCAATCTCCCAATTGGTACTGGAACCACCCGGCGATTGAAGGACTCCTCTGATCACACTGATAATGTCATGGACATCAATGTCAATACCGAACCCAATAATGTGGTATCATCCCACTTCACCAATGGGGTTGGCAACTATGGTTCGCAGCATTATGGTTACAATGGATGGTCGCCAATTAGCATGCAGCCGATCCCCTCGCAGTACGCCAACGGCCAGCCCAGGGCATGGTTGAAACAAGAGCAGGACAGCTCTGTGGTTACAGCGGCGCAGAACCTGCACAATCTACATCATTTTAGTTCCTTGGGCTACACCCACAACTTCTTCCAGCAATCTGATGTTCCAGACGTCACAGGTTTCGTTGATGCGCCTTCGAGGTCCAGTGACTCATACTCCTTCAGGTACAATGGAACAAATGGCTTTCATGGTCTCCCGGGTGGAATCAGCTATGCTATGCCGGTTGCGACAGCGGTGGACCAAGGTCAGGGCATCCATGGCTATGGAGAAGATGGTGTGGCAGGCATTGACACCACACATGACCTGTATGGCAGCCGTAATGTGTACTACCTTTCCGAGGGTTCGCTTCTTGCCGATGTCGAAAAAGAAGGCGACTATGGCCAATCTGTGGGGGGCAACAGCTGGGTTTTGCCGACACCGTAG
Protein Sequence
- >LOC_Os11g19060.1
MASITNWLGFSSSSFSGAGADPVLPHPPLQEWGSAYEGGGTVAAAGGEETAAPKLEDFLGMQVQQETAAAAAGHGRGGSSSVVGLSMIKNWLRSQPPPAVVGGEDAMMALAVSTSASPPVDATVPACISPDGMGSKAADGGGAAEAAAAAAAQRMKAAMDTFGQRTSIYRGVTKHRWTGRYEAHLWDNSCRREGQTRKGRQVNAGGYDKEEKAARAYDLAALKYWGTTTTTNFPVSNYEKELDEMKHMNRQEFVASLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFGTQEEAAEAYDIAAIKFRGLNAVTNFDMSRYDVKSIIESSNLPIGTGTTRRLKDSSDHTDNVMDINVNTEPNNVVSSHFTNGVGNYGSQHYGYNGWSPISMQPIPSQYANGQPRAWLKQEQDSSVVTAAQNLHNLHHFSSLGYTHNFFQQSDVPDVTGFVDAPSRSSDSYSFRYNGTNGFHGLPGGISYAMPVATAVDQGQGIHGYGEDGVAGIDTTHDLYGSRNVYYLSEGSLLADVEKEGDYGQSVGGNSWVLPTP*