Gene Details:

Functional Descriptions:

  • When genome editing to substitute mitosis for meiosis (MiMe)3,4 is combined with the expression of BBM1 in the egg cell, clonal progeny can be obtained that retain genome-wide parental heterozygosity.
  • In addition, the suppression of the function of OsASGR-BBML1 and its homologues in zygotes resulted in the developmental arrest, suggesting that OsASGR-BBML1 possesses an important role in initiating zygotic development.
  • Interestingly, ectopic expression of OsASGR-BBML1 in egg cells induced nuclear and cell divisions, indicating that exogenously expressed OsASGR-BBML1 converts the proliferation status of the egg cell from quiescent to active.
  • We identify OsYUCCA (OsYUC) auxin biosynthesis genes as direct targets of OsBBM1.
  • In somatic embryogenesis, exogenous auxin triggers OsBBM1 expression, which then activates endogenous auxin biosynthesis OsYUC genes.
  • We used gene editing, transcriptome profiling, and chromatin immunoprecipitation to determine the molecular players involved in embryo initiation downstream of OsBBM1.
  • Zygotic embryo initiation involves a partnership of male and female genomes, through which paternal OsBBM1 activates maternal OsYUC genes.
  • Ectopic OsBBM1 initiates somatic embryogenesis without exogenous auxins but requires functional OsYUC genes.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os11g19060.1
    CTCTCTCTCTCTCTCACTGTCCACATCTCTCCAACAAGAGAAATACTTTACACACTTGCAGCAACTCTTCCCCAGATGGCCTCCATCACCAACTGGCTCGGCTTCTCCTCCTCCTCCTTCTCCGGCGCCGGCGCCGACCCCGTCCTGCCCCACCCGCCGCTGCAAGAGTGGGGGAGCGCTTATGAGGGCGGCGGCACGGTGGCGGCCGCCGGCGGGGAGGAGACGGCGGCGCCGAAGCTGGAGGACTTCCTCGGCATGCAGGTGCAGCAGGAGACGGCCGCCGCGGCGGCGGGGCACGGCCGTGGAGGCAGCTCGTCGGTCGTTGGGCTGTCCATGATCAAGAACTGGCTACGCAGCCAGCCGCCGCCCGCGGTGGTTGGGGGAGAAGACGCTATGATGGCGCTCGCGGTGTCGACGTCGGCGTCGCCGCCGGTGGACGCGACGGTGCCGGCCTGCATTTCGCCGGATGGGATGGGGTCGAAGGCGGCCGACGGCGGCGGCGCGGCCGAGGCGGCGGCGGCGGCGGCGGCGCAGAGGATGAAGGCGGCCATGGACACGTTCGGGCAGCGGACGTCCATCTACCGGGGTGTCACCAAGCACAGGTGGACAGGAAGGTATGAAGCCCATCTTTGGGATAACAGCTGCAGAAGAGAAGGTCAGACTCGCAAAGGCAGACAAGTCAATGCAGGAGGATATGATAAGGAAGAAAAAGCTGCTAGGGCTTATGATTTGGCTGCCCTTAAATACTGGGGCACTACAACGACGACGAATTTTCCGGTAAGCAACTACGAAAAAGAGTTGGATGAAATGAAGCACATGAATAGGCAGGAATTTGTTGCATCCCTTAGAAGAAAAAGCAGTGGATTTTCACGTGGTGCTTCCATATATCGTGGTGTTACAAGACACCATCAGCATGGAAGGTGGCAAGCAAGGATAGGACGGGTGGCAGGAAACAAGGATCTGTATTTGGGCACATTTGGCACCCAAGAGGAAGCTGCAGAGGCATATGATATCGCTGCAATCAAATTCCGTGGTCTCAATGCTGTGACAAACTTTGACATGAGCCGGTACGATGTCAAGAGCATCATTGAAAGCAGCAATCTCCCAATTGGTACTGGAACCACCCGGCGATTGAAGGACTCCTCTGATCACACTGATAATGTCATGGACATCAATGTCAATACCGAACCCAATAATGTGGTATCATCCCACTTCACCAATGGGGTTGGCAACTATGGTTCGCAGCATTATGGTTACAATGGATGGTCGCCAATTAGCATGCAGCCGATCCCCTCGCAGTACGCCAACGGCCAGCCCAGGGCATGGTTGAAACAAGAGCAGGACAGCTCTGTGGTTACAGCGGCGCAGAACCTGCACAATCTACATCATTTTAGTTCCTTGGGCTACACCCACAACTTCTTCCAGCAATCTGATGTTCCAGACGTCACAGGTTTCGTTGATGCGCCTTCGAGGTCCAGTGACTCATACTCCTTCAGGTACAATGGAACAAATGGCTTTCATGGTCTCCCGGGTGGAATCAGCTATGCTATGCCGGTTGCGACAGCGGTGGACCAAGGTCAGGGCATCCATGGCTATGGAGAAGATGGTGTGGCAGGCATTGACACCACACATGACCTGTATGGCAGCCGTAATGTGTACTACCTTTCCGAGGGTTCGCTTCTTGCCGATGTCGAAAAAGAAGGCGACTATGGCCAATCTGTGGGGGGCAACAGCTGGGTTTTGCCGACACCGTAGTCAGAGATGGTGGAGAAGGCTGACAATGTGACGGTTTGCCATGGGATGCCTCTGTTCTCAGTCTGGAATGATGCTTAGCTGGGTGGAGAATTAGCTATAGTAGTTACTGAAAAGGTGATATTAACAGTTGTTGGGCAGATTGATTAACTGGGGGATATATAGTAGTCATACAAAGTCTTGCATGCTTGCTGATTTTGTATTGCTTTCTTATCACCACCATGCATGCAATCTATAGTTTTGCCTTTAGCTAGAAATATTAGCTCTAAATATCTCATGGAGATATGAATTTTATGCTGATGTTACTTAGGGCTCTTGATTATATATATGAGCTGATAATTCAGCCATTATCATCTCTTGCTTTCTGAGCTAGGTGATGTATAAATTTTCGTATGGAAGAGGTTTATGTTGCAGTACTGTTGATAATTCAGTCAGCAGGTTAAATTAAAGCTAAGTGTAACTATTGTGCTGGATCGACTTTGTTTCTTTTTCAAGATTTTACAATGTTGCACTTCGCCTGATGA
CDS Sequence
  • >LOC_Os11g19060.1
    ATGGCCTCCATCACCAACTGGCTCGGCTTCTCCTCCTCCTCCTTCTCCGGCGCCGGCGCCGACCCCGTCCTGCCCCACCCGCCGCTGCAAGAGTGGGGGAGCGCTTATGAGGGCGGCGGCACGGTGGCGGCCGCCGGCGGGGAGGAGACGGCGGCGCCGAAGCTGGAGGACTTCCTCGGCATGCAGGTGCAGCAGGAGACGGCCGCCGCGGCGGCGGGGCACGGCCGTGGAGGCAGCTCGTCGGTCGTTGGGCTGTCCATGATCAAGAACTGGCTACGCAGCCAGCCGCCGCCCGCGGTGGTTGGGGGAGAAGACGCTATGATGGCGCTCGCGGTGTCGACGTCGGCGTCGCCGCCGGTGGACGCGACGGTGCCGGCCTGCATTTCGCCGGATGGGATGGGGTCGAAGGCGGCCGACGGCGGCGGCGCGGCCGAGGCGGCGGCGGCGGCGGCGGCGCAGAGGATGAAGGCGGCCATGGACACGTTCGGGCAGCGGACGTCCATCTACCGGGGTGTCACCAAGCACAGGTGGACAGGAAGGTATGAAGCCCATCTTTGGGATAACAGCTGCAGAAGAGAAGGTCAGACTCGCAAAGGCAGACAAGTCAATGCAGGAGGATATGATAAGGAAGAAAAAGCTGCTAGGGCTTATGATTTGGCTGCCCTTAAATACTGGGGCACTACAACGACGACGAATTTTCCGGTAAGCAACTACGAAAAAGAGTTGGATGAAATGAAGCACATGAATAGGCAGGAATTTGTTGCATCCCTTAGAAGAAAAAGCAGTGGATTTTCACGTGGTGCTTCCATATATCGTGGTGTTACAAGACACCATCAGCATGGAAGGTGGCAAGCAAGGATAGGACGGGTGGCAGGAAACAAGGATCTGTATTTGGGCACATTTGGCACCCAAGAGGAAGCTGCAGAGGCATATGATATCGCTGCAATCAAATTCCGTGGTCTCAATGCTGTGACAAACTTTGACATGAGCCGGTACGATGTCAAGAGCATCATTGAAAGCAGCAATCTCCCAATTGGTACTGGAACCACCCGGCGATTGAAGGACTCCTCTGATCACACTGATAATGTCATGGACATCAATGTCAATACCGAACCCAATAATGTGGTATCATCCCACTTCACCAATGGGGTTGGCAACTATGGTTCGCAGCATTATGGTTACAATGGATGGTCGCCAATTAGCATGCAGCCGATCCCCTCGCAGTACGCCAACGGCCAGCCCAGGGCATGGTTGAAACAAGAGCAGGACAGCTCTGTGGTTACAGCGGCGCAGAACCTGCACAATCTACATCATTTTAGTTCCTTGGGCTACACCCACAACTTCTTCCAGCAATCTGATGTTCCAGACGTCACAGGTTTCGTTGATGCGCCTTCGAGGTCCAGTGACTCATACTCCTTCAGGTACAATGGAACAAATGGCTTTCATGGTCTCCCGGGTGGAATCAGCTATGCTATGCCGGTTGCGACAGCGGTGGACCAAGGTCAGGGCATCCATGGCTATGGAGAAGATGGTGTGGCAGGCATTGACACCACACATGACCTGTATGGCAGCCGTAATGTGTACTACCTTTCCGAGGGTTCGCTTCTTGCCGATGTCGAAAAAGAAGGCGACTATGGCCAATCTGTGGGGGGCAACAGCTGGGTTTTGCCGACACCGTAG
Protein Sequence
  • >LOC_Os11g19060.1
    MASITNWLGFSSSSFSGAGADPVLPHPPLQEWGSAYEGGGTVAAAGGEETAAPKLEDFLGMQVQQETAAAAAGHGRGGSSSVVGLSMIKNWLRSQPPPAVVGGEDAMMALAVSTSASPPVDATVPACISPDGMGSKAADGGGAAEAAAAAAAQRMKAAMDTFGQRTSIYRGVTKHRWTGRYEAHLWDNSCRREGQTRKGRQVNAGGYDKEEKAARAYDLAALKYWGTTTTTNFPVSNYEKELDEMKHMNRQEFVASLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFGTQEEAAEAYDIAAIKFRGLNAVTNFDMSRYDVKSIIESSNLPIGTGTTRRLKDSSDHTDNVMDINVNTEPNNVVSSHFTNGVGNYGSQHYGYNGWSPISMQPIPSQYANGQPRAWLKQEQDSSVVTAAQNLHNLHHFSSLGYTHNFFQQSDVPDVTGFVDAPSRSSDSYSFRYNGTNGFHGLPGGISYAMPVATAVDQGQGIHGYGEDGVAGIDTTHDLYGSRNVYYLSEGSLLADVEKEGDYGQSVGGNSWVLPTP*