Gene Details:

Functional Descriptions:

  • While OsCPK24 knockdown plants exhibited increased sensitivity to cold compared to wild type, OsCPK24-overexpressing plants exhibited increased cold tolerance.
  • Together, our results suggest that OsCPK24 functions as a positive regulator of cold stress tolerance in rice, a process mediated by calcium signaling and involving phosphorylation and the inhibition of OsGrx10 to sustain higher glutathione levels.
  • The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice.
  • Plants overexpressing OsCPK24 exhibited increased accumulation of proline (an osmoprotectant) and glutathione (an antioxidant) and maintained a higher GSH/GSSG (reduced glutathione to oxidized glutathione) ratio during cold stress compared to wild type.
  • In addition to these effects in responses to cold stress, we found that the kinase activity of OsCPK24 varied under different calcium concentrations.
  • Further, OsCPK24 phosphorylated OsGrx10, a glutathione-dependent thioltransferase, at rates modulated by changes in calcium concentration.
  • Conclusions: OsANN4 plays a crucial role in the ABA response, partially by modulating ROS production, mediating Ca2+ influx or interacting with OsCDPK24.
  • This study revealed the molecular mechanism of ET-induced resistance to Xoo in rice via OsCPK24, which provided a basis for the development of new bactericides based on the OsCPK24 protein.
  • In addition, the biological function of OsCPK24 as a mediator for rice resistance to Xoo was determined through the anti-Xoo phenotypic test of OsCPK24 transgenic rice and the affinity analysis of the OsCPK24 recombinant protein in vitro and ET.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os11g07040.1
    GTTCTTGTCTTGTCACACCCATGGAGGAGGAGAAAAAGGCATAGATACGACCTGGCTGCCCTCACAAAAATACTTCACCAAACCACCCTCCTCCTCCTCCCCCTTTACCATTTCCAAGTTCCAACCCCCAAAAAAAATCCAACCAAAAAGCGGAGATTTTCCAACTCTTATCATCTCCTCCTCCTCCTCCTCGTCATCGTCATCACCAGCACACACCCGAGAGAAAAAAATCCTATAAAGAAATGGATTGAGCCATCACCCAACGCCGCCAAGATCCTGTCCTCTTGTGTGCGCAACCTGTAGCTCCACCTCCACGCAGGATCAACACCCAAATCCCCCCATCTTCTTTTCTTTTTCGTTTCGTTTCTGCAAGATTTGCATCTTCTTCTTCTTCCTCCGAGGAGTGAGTGACACCTGAGATAGCGAGAGCGAGATTTTCTTCGGTTTTTTTGTTCCTCTTTTTGGCCATAAACCTCCCATAAAGCTCGGTTTCTTGGTTCGTTTTTTCTTTCTTTCTTCCTCTCCTTCGAGAATCGCCCGAGTGTGAAATCCAAGATCCCGAATTCCGTGTCTTCTTCTTCTTCCGTAGAAATCCCGGGGTGGGCAAAGCGGTGGGATTTCTGGAGAGGGGGAGAGCGCTACAAGGCAAGAAGCTTGGGTGTCCGTAGTCCGTACGGTTCTTGATCTGGGGTCGCCGCCAATGCAGCCGGACCCGAGCGGGAGCGGCGGCGACGGCAACGCCAATGCGAAGGCGAAGCTGGCGCCGCCGCCGGTGACGGCGGCGGGAGGTCGGCCGGTGTCGGTGCTGCCGCACAAGACGGCGAACGTGCGCGACCACTACCGCATCGGGAAGAAGCTCGGGCAGGGGCAGTTCGGGACGACGTACCTGTGCGTGGACAAGGCGAGCGGCGGCGAGTTCGCGTGCAAGTCCATCCCCAAGCGGAAGCTGCTGTGCCGGGAGGACTACGAGGACGTGTGGCGCGAGATCCAGATCATGCACCACCTCTCCGAGCACCCCAACGTCGTCCGCATCCGCGGCGCCTACGAGGACGCGCTGTTCGTCCACATCGTCATGGAGCTCTGCGCCGGCGGCGAGCTCTTCGACCGCATCGTCGCCAAGGGCCACTACACCGAGCGCGCCGCCGCGCAGCTCATCCGCACCATCGTCGCCGTCGTCGAGGGATGCCACTCGCTCGGCGTCATGCACCGGGACCTCAAGCCCGAGAACTTCCTCTTCGCCAGCGCCGCCGAGGACGCCCCCCTCAAGGCCACTGATTTCGGCCTCTCCATGTTCTACAAGCCTGGTGATAAGTTCTCTGATGTTGTTGGGAGCCCCTATTATGTTGCACCTGAGGTACTTCAGAAATGCTATGGTCCAGAATCTGATGTCTGGAGTGCTGGGGTAATTCTTTACATTTTGCTATGTGGTGTTCCACCATTTTGGGCAGAAACTGAAGCTGGAATTTTCAGGCAGATTCTACGAGGCAAACTTGATTTTGAATCTGAACCCTGGCCTAGCATCTCTGACAGTGCTAAAGATCTAGTCCGTAATATGCTTTGCCGGGATCCTACAAAGAGACTTACAGCCCATGAGGTTCTCTGTCATCCATGGATTGTTGATGATGCTGTGGCACCTGACAAGCCTATTGATTCTGCTGTTTTGTCAAGACTGAAGCACTTTTCGGCAATGAACAAGCTCAAGAAGATGGCATTGAGGGTTATTGCTGAAAGCCTGTCTGAGGAAGAGATTGGAGGACTAAAGGAGCTATTCAAAATGATTGATACTGACGATAGTGGAACTATAACTTTTGATGAGCTGAAAGAGGGCTTGAAAAGGGTGGGTTCAGAATTAACAGAACATGAAATCCAGGCTTTAATGGAAGCGGCTGATATCGACAACAGTGGAACCATCGACTATGGTGAATTCATTGCTGCTACTCTCCACATGAATAAGCTAGAGAGGGAGGAAAACTTAGTATCAGCATTCTCATTTTTCGACAAAGATGGAAGTGGTTTCATAACTATTGATGAGCTGTCACAAGCATGCCGCGAATTTGGCCTTGATGATCTTCACCTTGAGGATATGATTAAAGATGTTGATCAAAACAATGACGGACAAATTGACTACAGCGAGTTCACGGCGATGATGAGAAAGGGCAATGCTGGTGGAGCTGGAAGGAGAACCATGAGGAACAGCTTGCAGTTGAATCTTGGTGAGATCTTGAACCCCAGCAACAGCTAAACTCCTACAGGAAGGCCATGGCTGTTACATGCCATCATTGTCTGAATCTCTGCACGCCTCATCAGCTGGACTAATCTCTAAGAGGGCAAAGCCGTTGAGTGAATTGTGCTTTGGCGTTACATGAGACGCTGAAGAATTTTCGTAGCAGTAATAGCTACAGCTACTAGAGTGTAAGAGAAATTGGCGTTATGTGATCGTAATATCCTATCAGTGTAACTGATCTTAGATGATGCTGGAGATGATAGTACAATGAACAGCTTATATGTATTCCTGTCCTTGTAGACTGAACATTGTTGATAGTTGGGGAATGCTCTTGGCATCAATTCTGTCTCTTCTATCTATTATTCTATCGTTAGTACAGGTTGTTGATGTTAAATGTC
CDS Sequence
  • >LOC_Os11g07040.1
    ATGCAGCCGGACCCGAGCGGGAGCGGCGGCGACGGCAACGCCAATGCGAAGGCGAAGCTGGCGCCGCCGCCGGTGACGGCGGCGGGAGGTCGGCCGGTGTCGGTGCTGCCGCACAAGACGGCGAACGTGCGCGACCACTACCGCATCGGGAAGAAGCTCGGGCAGGGGCAGTTCGGGACGACGTACCTGTGCGTGGACAAGGCGAGCGGCGGCGAGTTCGCGTGCAAGTCCATCCCCAAGCGGAAGCTGCTGTGCCGGGAGGACTACGAGGACGTGTGGCGCGAGATCCAGATCATGCACCACCTCTCCGAGCACCCCAACGTCGTCCGCATCCGCGGCGCCTACGAGGACGCGCTGTTCGTCCACATCGTCATGGAGCTCTGCGCCGGCGGCGAGCTCTTCGACCGCATCGTCGCCAAGGGCCACTACACCGAGCGCGCCGCCGCGCAGCTCATCCGCACCATCGTCGCCGTCGTCGAGGGATGCCACTCGCTCGGCGTCATGCACCGGGACCTCAAGCCCGAGAACTTCCTCTTCGCCAGCGCCGCCGAGGACGCCCCCCTCAAGGCCACTGATTTCGGCCTCTCCATGTTCTACAAGCCTGGTGATAAGTTCTCTGATGTTGTTGGGAGCCCCTATTATGTTGCACCTGAGGTACTTCAGAAATGCTATGGTCCAGAATCTGATGTCTGGAGTGCTGGGGTAATTCTTTACATTTTGCTATGTGGTGTTCCACCATTTTGGGCAGAAACTGAAGCTGGAATTTTCAGGCAGATTCTACGAGGCAAACTTGATTTTGAATCTGAACCCTGGCCTAGCATCTCTGACAGTGCTAAAGATCTAGTCCGTAATATGCTTTGCCGGGATCCTACAAAGAGACTTACAGCCCATGAGGTTCTCTGTCATCCATGGATTGTTGATGATGCTGTGGCACCTGACAAGCCTATTGATTCTGCTGTTTTGTCAAGACTGAAGCACTTTTCGGCAATGAACAAGCTCAAGAAGATGGCATTGAGGGTTATTGCTGAAAGCCTGTCTGAGGAAGAGATTGGAGGACTAAAGGAGCTATTCAAAATGATTGATACTGACGATAGTGGAACTATAACTTTTGATGAGCTGAAAGAGGGCTTGAAAAGGGTGGGTTCAGAATTAACAGAACATGAAATCCAGGCTTTAATGGAAGCGGCTGATATCGACAACAGTGGAACCATCGACTATGGTGAATTCATTGCTGCTACTCTCCACATGAATAAGCTAGAGAGGGAGGAAAACTTAGTATCAGCATTCTCATTTTTCGACAAAGATGGAAGTGGTTTCATAACTATTGATGAGCTGTCACAAGCATGCCGCGAATTTGGCCTTGATGATCTTCACCTTGAGGATATGATTAAAGATGTTGATCAAAACAATGACGGACAAATTGACTACAGCGAGTTCACGGCGATGATGAGAAAGGGCAATGCTGGTGGAGCTGGAAGGAGAACCATGAGGAACAGCTTGCAGTTGAATCTTGGTGAGATCTTGAACCCCAGCAACAGCTAA
Protein Sequence
  • >LOC_Os11g07040.1
    MQPDPSGSGGDGNANAKAKLAPPPVTAAGGRPVSVLPHKTANVRDHYRIGKKLGQGQFGTTYLCVDKASGGEFACKSIPKRKLLCREDYEDVWREIQIMHHLSEHPNVVRIRGAYEDALFVHIVMELCAGGELFDRIVAKGHYTERAAAQLIRTIVAVVEGCHSLGVMHRDLKPENFLFASAAEDAPLKATDFGLSMFYKPGDKFSDVVGSPYYVAPEVLQKCYGPESDVWSAGVILYILLCGVPPFWAETEAGIFRQILRGKLDFESEPWPSISDSAKDLVRNMLCRDPTKRLTAHEVLCHPWIVDDAVAPDKPIDSAVLSRLKHFSAMNKLKKMALRVIAESLSEEEIGGLKELFKMIDTDDSGTITFDELKEGLKRVGSELTEHEIQALMEAADIDNSGTIDYGEFIAATLHMNKLEREENLVSAFSFFDKDGSGFITIDELSQACREFGLDDLHLEDMIKDVDQNNDGQIDYSEFTAMMRKGNAGGAGRRTMRNSLQLNLGEILNPSNS*