Information report for OsCPK24;OsCDPK14
Gene Details
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Functional Descriptions
- While OsCPK24 knockdown plants exhibited increased sensitivity to cold compared to wild type, OsCPK24-overexpressing plants exhibited increased cold tolerance.
- Together, our results suggest that OsCPK24 functions as a positive regulator of cold stress tolerance in rice, a process mediated by calcium signaling and involving phosphorylation and the inhibition of OsGrx10 to sustain higher glutathione levels.
- The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice.
- Plants overexpressing OsCPK24 exhibited increased accumulation of proline (an osmoprotectant) and glutathione (an antioxidant) and maintained a higher GSH/GSSG (reduced glutathione to oxidized glutathione) ratio during cold stress compared to wild type.
- In addition to these effects in responses to cold stress, we found that the kinase activity of OsCPK24 varied under different calcium concentrations.
- Further, OsCPK24 phosphorylated OsGrx10, a glutathione-dependent thioltransferase, at rates modulated by changes in calcium concentration.
- Conclusions: OsANN4 plays a crucial role in the ABA response, partially by modulating ROS production, mediating Ca2+ influx or interacting with OsCDPK24.
- This study revealed the molecular mechanism of ET-induced resistance to Xoo in rice via OsCPK24, which provided a basis for the development of new bactericides based on the OsCPK24 protein.
- In addition, the biological function of OsCPK24 as a mediator for rice resistance to Xoo was determined through the anti-Xoo phenotypic test of OsCPK24 transgenic rice and the affinity analysis of the OsCPK24 recombinant protein in vitro and ET.
Functional Keywords
- tolerance , cold-tolerance , cold-stress , stress , Kinase , stress-tolerance , stress-response , calcium , ABA , development , resistance , xoo , Xoo
Literature and News
- The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice . DOI: 10.1111/jipb.12614 ; PMID: 29193704
- Cloning and biochemical properties of CDPK gene OsCDPK14 from rice . DOI: 10.1016/j.jplph.2004.12.010 ; PMID: 16255173
- Dynamic genome-wide association analysis and identification of candidate genes involved in anaerobic germination tolerance in rice . DOI: 10.1186/s12284-020-00444-x ; PMID: 33409869
- OsANN4 modulates ROS production and mediates Ca2+ influx in response to ABA . DOI: 10.1186/s12870-021-03248-3 ; PMID: 34663209
- Immune Mechanism of Ethylicin-Induced Resistance to Xanthomonas oryzae pv. oryzae in Rice . DOI: 10.1021/acs.jafc.2c07385 ; PMID: 36591973
Gene Resources
- UniProt: Q53P85
- EMBL: AC128643, AP008217, AP014967
- AlphaFoldDB: Q53P85
- EnsemblPlants: Os11t0171500-01
- Gramene: Os11t0171500-01
- KEGG: dosa:Os11g0171500
- Orthologous matrix: PFMGANE
- InterPro: IPR000719, IPR002048, IPR008271
- PANTHER: PTHR24349
- SUPFAM: SSF47473, SSF56112
- PROSITE: PS00018, PS00107, PS00108
- Gene3D: 1.10.238.10, 1.10.510.10, 3.30.200.20
- OrthoDB: Q53P85
- SWISS-MODEL: Q53P85
- Conserved Domain Database: cd00051, cd05117
- eggNOG: KOG0032
Sequences
cDNA Sequence
- >LOC_Os11g07040.1
GTTCTTGTCTTGTCACACCCATGGAGGAGGAGAAAAAGGCATAGATACGACCTGGCTGCCCTCACAAAAATACTTCACCAAACCACCCTCCTCCTCCTCCCCCTTTACCATTTCCAAGTTCCAACCCCCAAAAAAAATCCAACCAAAAAGCGGAGATTTTCCAACTCTTATCATCTCCTCCTCCTCCTCCTCGTCATCGTCATCACCAGCACACACCCGAGAGAAAAAAATCCTATAAAGAAATGGATTGAGCCATCACCCAACGCCGCCAAGATCCTGTCCTCTTGTGTGCGCAACCTGTAGCTCCACCTCCACGCAGGATCAACACCCAAATCCCCCCATCTTCTTTTCTTTTTCGTTTCGTTTCTGCAAGATTTGCATCTTCTTCTTCTTCCTCCGAGGAGTGAGTGACACCTGAGATAGCGAGAGCGAGATTTTCTTCGGTTTTTTTGTTCCTCTTTTTGGCCATAAACCTCCCATAAAGCTCGGTTTCTTGGTTCGTTTTTTCTTTCTTTCTTCCTCTCCTTCGAGAATCGCCCGAGTGTGAAATCCAAGATCCCGAATTCCGTGTCTTCTTCTTCTTCCGTAGAAATCCCGGGGTGGGCAAAGCGGTGGGATTTCTGGAGAGGGGGAGAGCGCTACAAGGCAAGAAGCTTGGGTGTCCGTAGTCCGTACGGTTCTTGATCTGGGGTCGCCGCCAATGCAGCCGGACCCGAGCGGGAGCGGCGGCGACGGCAACGCCAATGCGAAGGCGAAGCTGGCGCCGCCGCCGGTGACGGCGGCGGGAGGTCGGCCGGTGTCGGTGCTGCCGCACAAGACGGCGAACGTGCGCGACCACTACCGCATCGGGAAGAAGCTCGGGCAGGGGCAGTTCGGGACGACGTACCTGTGCGTGGACAAGGCGAGCGGCGGCGAGTTCGCGTGCAAGTCCATCCCCAAGCGGAAGCTGCTGTGCCGGGAGGACTACGAGGACGTGTGGCGCGAGATCCAGATCATGCACCACCTCTCCGAGCACCCCAACGTCGTCCGCATCCGCGGCGCCTACGAGGACGCGCTGTTCGTCCACATCGTCATGGAGCTCTGCGCCGGCGGCGAGCTCTTCGACCGCATCGTCGCCAAGGGCCACTACACCGAGCGCGCCGCCGCGCAGCTCATCCGCACCATCGTCGCCGTCGTCGAGGGATGCCACTCGCTCGGCGTCATGCACCGGGACCTCAAGCCCGAGAACTTCCTCTTCGCCAGCGCCGCCGAGGACGCCCCCCTCAAGGCCACTGATTTCGGCCTCTCCATGTTCTACAAGCCTGGTGATAAGTTCTCTGATGTTGTTGGGAGCCCCTATTATGTTGCACCTGAGGTACTTCAGAAATGCTATGGTCCAGAATCTGATGTCTGGAGTGCTGGGGTAATTCTTTACATTTTGCTATGTGGTGTTCCACCATTTTGGGCAGAAACTGAAGCTGGAATTTTCAGGCAGATTCTACGAGGCAAACTTGATTTTGAATCTGAACCCTGGCCTAGCATCTCTGACAGTGCTAAAGATCTAGTCCGTAATATGCTTTGCCGGGATCCTACAAAGAGACTTACAGCCCATGAGGTTCTCTGTCATCCATGGATTGTTGATGATGCTGTGGCACCTGACAAGCCTATTGATTCTGCTGTTTTGTCAAGACTGAAGCACTTTTCGGCAATGAACAAGCTCAAGAAGATGGCATTGAGGGTTATTGCTGAAAGCCTGTCTGAGGAAGAGATTGGAGGACTAAAGGAGCTATTCAAAATGATTGATACTGACGATAGTGGAACTATAACTTTTGATGAGCTGAAAGAGGGCTTGAAAAGGGTGGGTTCAGAATTAACAGAACATGAAATCCAGGCTTTAATGGAAGCGGCTGATATCGACAACAGTGGAACCATCGACTATGGTGAATTCATTGCTGCTACTCTCCACATGAATAAGCTAGAGAGGGAGGAAAACTTAGTATCAGCATTCTCATTTTTCGACAAAGATGGAAGTGGTTTCATAACTATTGATGAGCTGTCACAAGCATGCCGCGAATTTGGCCTTGATGATCTTCACCTTGAGGATATGATTAAAGATGTTGATCAAAACAATGACGGACAAATTGACTACAGCGAGTTCACGGCGATGATGAGAAAGGGCAATGCTGGTGGAGCTGGAAGGAGAACCATGAGGAACAGCTTGCAGTTGAATCTTGGTGAGATCTTGAACCCCAGCAACAGCTAAACTCCTACAGGAAGGCCATGGCTGTTACATGCCATCATTGTCTGAATCTCTGCACGCCTCATCAGCTGGACTAATCTCTAAGAGGGCAAAGCCGTTGAGTGAATTGTGCTTTGGCGTTACATGAGACGCTGAAGAATTTTCGTAGCAGTAATAGCTACAGCTACTAGAGTGTAAGAGAAATTGGCGTTATGTGATCGTAATATCCTATCAGTGTAACTGATCTTAGATGATGCTGGAGATGATAGTACAATGAACAGCTTATATGTATTCCTGTCCTTGTAGACTGAACATTGTTGATAGTTGGGGAATGCTCTTGGCATCAATTCTGTCTCTTCTATCTATTATTCTATCGTTAGTACAGGTTGTTGATGTTAAATGTC
CDS Sequence
- >LOC_Os11g07040.1
ATGCAGCCGGACCCGAGCGGGAGCGGCGGCGACGGCAACGCCAATGCGAAGGCGAAGCTGGCGCCGCCGCCGGTGACGGCGGCGGGAGGTCGGCCGGTGTCGGTGCTGCCGCACAAGACGGCGAACGTGCGCGACCACTACCGCATCGGGAAGAAGCTCGGGCAGGGGCAGTTCGGGACGACGTACCTGTGCGTGGACAAGGCGAGCGGCGGCGAGTTCGCGTGCAAGTCCATCCCCAAGCGGAAGCTGCTGTGCCGGGAGGACTACGAGGACGTGTGGCGCGAGATCCAGATCATGCACCACCTCTCCGAGCACCCCAACGTCGTCCGCATCCGCGGCGCCTACGAGGACGCGCTGTTCGTCCACATCGTCATGGAGCTCTGCGCCGGCGGCGAGCTCTTCGACCGCATCGTCGCCAAGGGCCACTACACCGAGCGCGCCGCCGCGCAGCTCATCCGCACCATCGTCGCCGTCGTCGAGGGATGCCACTCGCTCGGCGTCATGCACCGGGACCTCAAGCCCGAGAACTTCCTCTTCGCCAGCGCCGCCGAGGACGCCCCCCTCAAGGCCACTGATTTCGGCCTCTCCATGTTCTACAAGCCTGGTGATAAGTTCTCTGATGTTGTTGGGAGCCCCTATTATGTTGCACCTGAGGTACTTCAGAAATGCTATGGTCCAGAATCTGATGTCTGGAGTGCTGGGGTAATTCTTTACATTTTGCTATGTGGTGTTCCACCATTTTGGGCAGAAACTGAAGCTGGAATTTTCAGGCAGATTCTACGAGGCAAACTTGATTTTGAATCTGAACCCTGGCCTAGCATCTCTGACAGTGCTAAAGATCTAGTCCGTAATATGCTTTGCCGGGATCCTACAAAGAGACTTACAGCCCATGAGGTTCTCTGTCATCCATGGATTGTTGATGATGCTGTGGCACCTGACAAGCCTATTGATTCTGCTGTTTTGTCAAGACTGAAGCACTTTTCGGCAATGAACAAGCTCAAGAAGATGGCATTGAGGGTTATTGCTGAAAGCCTGTCTGAGGAAGAGATTGGAGGACTAAAGGAGCTATTCAAAATGATTGATACTGACGATAGTGGAACTATAACTTTTGATGAGCTGAAAGAGGGCTTGAAAAGGGTGGGTTCAGAATTAACAGAACATGAAATCCAGGCTTTAATGGAAGCGGCTGATATCGACAACAGTGGAACCATCGACTATGGTGAATTCATTGCTGCTACTCTCCACATGAATAAGCTAGAGAGGGAGGAAAACTTAGTATCAGCATTCTCATTTTTCGACAAAGATGGAAGTGGTTTCATAACTATTGATGAGCTGTCACAAGCATGCCGCGAATTTGGCCTTGATGATCTTCACCTTGAGGATATGATTAAAGATGTTGATCAAAACAATGACGGACAAATTGACTACAGCGAGTTCACGGCGATGATGAGAAAGGGCAATGCTGGTGGAGCTGGAAGGAGAACCATGAGGAACAGCTTGCAGTTGAATCTTGGTGAGATCTTGAACCCCAGCAACAGCTAA
Protein Sequence
- >LOC_Os11g07040.1
MQPDPSGSGGDGNANAKAKLAPPPVTAAGGRPVSVLPHKTANVRDHYRIGKKLGQGQFGTTYLCVDKASGGEFACKSIPKRKLLCREDYEDVWREIQIMHHLSEHPNVVRIRGAYEDALFVHIVMELCAGGELFDRIVAKGHYTERAAAQLIRTIVAVVEGCHSLGVMHRDLKPENFLFASAAEDAPLKATDFGLSMFYKPGDKFSDVVGSPYYVAPEVLQKCYGPESDVWSAGVILYILLCGVPPFWAETEAGIFRQILRGKLDFESEPWPSISDSAKDLVRNMLCRDPTKRLTAHEVLCHPWIVDDAVAPDKPIDSAVLSRLKHFSAMNKLKKMALRVIAESLSEEEIGGLKELFKMIDTDDSGTITFDELKEGLKRVGSELTEHEIQALMEAADIDNSGTIDYGEFIAATLHMNKLEREENLVSAFSFFDKDGSGFITIDELSQACREFGLDDLHLEDMIKDVDQNNDGQIDYSEFTAMMRKGNAGGAGRRTMRNSLQLNLGEILNPSNS*