Gene Details:

Functional Descriptions:

  • The OsDMC1-RNAi lines grew normally during their vegetative phase but showed spikelet and pollen sterility.
  • The sterility phenotypes were associated with down-regulated OsDMC1 transcript and protein levels mediated by RNAi.
  • OsDMC1 was expressed at high-levels in reproductive organs, low-levels in roots, and undetectable levels in leaves and seedlings.
  • Isolation and characterization of OsDMC1 , the rice homologue of the yeast DMC1 gene essential for meiosis.
  • Further cytological observations of male meiocytes revealed that knock-down of OsDMC1 led to defects in bivalent formation and subsequent unequal chromosome segregation and irregular spore generation, and induced changes in male meiotic progression.
  • DNA binding and pairing activities of Oryza sativa disrupted meiotic cDNA1 (OsDMC1) from rice have been reported earlier.
  • A cloned cDNA corresponding to OsDMC1 from rice anther tissue was expressed in Escherichia coli.
  • Here, we analyzed the function of OsDMC1 in meiosis using an RNA interference approach.
  • These data indicate that OsDMC1 is essential for rice meiosis and plays an important role in homologous pairing.
  • These biochemical differences between the DMC1A and DMC1B proteins may provide important insight into their functional differences during meiosis in rice.
  • Moreover, OsDMC1 was not detected in pairing-defective mutants, such as pair2, pair3, Oscom1 and Osrad51c, while it was loaded onto meiotic chromosomes in zep1, Osmer3, Oszip4 and Oshei10.
  • The reduced number of bivalents and abnormal OsHEI10 foci in Osdmc1a OsDMC1B establishes an essential role for OsDMC1 in crossover formation.
  • These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination.
  • In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and in vitro OsBRCA2 directly interacts with OsRAD51 and OsDMC1.
  • In the homologous recombination pathway, the detailed mechanisms of how OsRAD51 and OsDMC1 work in rice meiosis remain to be explored.
  • In addition, both OsRAD51 and OsDMC1 can interact with OsFIGL1 and OsBRCA2, two important components in rice meiosis.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os11g04954.1
    AACGCAACGGCACAAGCACAACACCACTTCGAGTGTGTTTGTCGTCTCGCCTCTGCTGCTTCCTTCTCCTACAAGCCGCCGCTCCGACCTCGCCGTCGCCGGTCGCCGGCCGCCTCCGCCTACAGGTGCGGGGGGTCTGTGTGAGCAGATGGCGCCGTCCAAGCAGTACGACGAAGGCGGGCAGCTCCAGCTCATGGATGCAGAGAGGATCGAGGAGGAGGAGGAGTGCTTCGAGTCCATCGACAAGTTAATCTCGCAAGGGATAAACTCTGGAGATGTGAAGAAGCTGCAGGATGCTGGTATCTACACTTGCAATGGCCTCATGATGCATACAAAGAAGAGCCTGACAGGAATCAAGGGATTATCTGAAGCAAAGGTAGACAAGATCTGTGAAGCAGCTGAAAAGCTTCTGAGCCAGGGCTTCATGACAGGAAGTGATCTCCTTATCAAGCGGAAGTCTGTTGTTCGGATCACCACTGGGAGCCAGGCACTTGATGAGCTGCTTGGCGGAGGGATTGAAACACTCTGCATCACAGAGGCATTTGGAGAGTTCCGATCAGGGAAGACCCAGTTGGCTCACACTCTATGTGTCTCCACTCAGCTTCCAATTCACATGCATGGGGGGAACGGGAAGGTTGCTTACATTGACACTGAGGGAACATTCCGGCCTGAACGAATTGTGCCAATTGCTGAGAGATTTGGGATGGATGCCAATGCTGTTCTTGATAACATCATATATGCTCGTGCATACACCTATGAGCACCAGTACAACTTGCTCCTTGGCCTCGCTGCCAAGATGGCTGAAGAGCCTTTCAGGCTTCTGATTGTGGATTCTGTGATTGCACTATTCCGTGTTGATTTCAGTGGCAGGGGTGAACTTGCAGAGCGCCAGCAAAAATTGGCACAGATGCTCTCCCGCCTTACTAAGATAGCTGAGGAGTTCAATGTTGCAGTGTACATCACCAACCAAGTGATTGCGGACCCAGGTGGTGGAATGTTCATAACTGACCCGAAGAAACCTGCGGGAGGCCACGTTCTGGCGCATGCAGCCACCATCCGGTTGATGCTGAGGAAAGGCAAAGGAGAGCAGCGTGTCTGCAAGATCTTTGATGCCCCTAACCTGCCTGAGGGAGAAGCTGTTTTCCAGGTAACATCAGGTGGAATAATGGATGCGAAAGACTGAATGTTCACTGAGGGTGCTTCCTGTGTTCAAATTGTTCAGGTTTCTCCAAGTAAATTTCTGCATAGGCCATATAGGTTAGGTACATTGATAAAAGAATACAACTACTGCTGCTGCTATGTTTTTGCCACTGCTGAGGAAAGAGGCAACCTCATTTATCAAGACGGTTATATCTCACATTGGGTATTATGAGTTATGTGATCAACAGTTGTACTGAAACAAGTGCTAGTATTGTCTTAAGTGAAAATATAAGAGGTTAACATGTTGGTTTCCCCAGATACTATTTTTTGTACCTGCTCGTGCACAATTTATACAATCGGTCTTAAACTGCTGT
CDS Sequence
  • >LOC_Os11g04954.1
    ATGGCGCCGTCCAAGCAGTACGACGAAGGCGGGCAGCTCCAGCTCATGGATGCAGAGAGGATCGAGGAGGAGGAGGAGTGCTTCGAGTCCATCGACAAGTTAATCTCGCAAGGGATAAACTCTGGAGATGTGAAGAAGCTGCAGGATGCTGGTATCTACACTTGCAATGGCCTCATGATGCATACAAAGAAGAGCCTGACAGGAATCAAGGGATTATCTGAAGCAAAGGTAGACAAGATCTGTGAAGCAGCTGAAAAGCTTCTGAGCCAGGGCTTCATGACAGGAAGTGATCTCCTTATCAAGCGGAAGTCTGTTGTTCGGATCACCACTGGGAGCCAGGCACTTGATGAGCTGCTTGGCGGAGGGATTGAAACACTCTGCATCACAGAGGCATTTGGAGAGTTCCGATCAGGGAAGACCCAGTTGGCTCACACTCTATGTGTCTCCACTCAGCTTCCAATTCACATGCATGGGGGGAACGGGAAGGTTGCTTACATTGACACTGAGGGAACATTCCGGCCTGAACGAATTGTGCCAATTGCTGAGAGATTTGGGATGGATGCCAATGCTGTTCTTGATAACATCATATATGCTCGTGCATACACCTATGAGCACCAGTACAACTTGCTCCTTGGCCTCGCTGCCAAGATGGCTGAAGAGCCTTTCAGGCTTCTGATTGTGGATTCTGTGATTGCACTATTCCGTGTTGATTTCAGTGGCAGGGGTGAACTTGCAGAGCGCCAGCAAAAATTGGCACAGATGCTCTCCCGCCTTACTAAGATAGCTGAGGAGTTCAATGTTGCAGTGTACATCACCAACCAAGTGATTGCGGACCCAGGTGGTGGAATGTTCATAACTGACCCGAAGAAACCTGCGGGAGGCCACGTTCTGGCGCATGCAGCCACCATCCGGTTGATGCTGAGGAAAGGCAAAGGAGAGCAGCGTGTCTGCAAGATCTTTGATGCCCCTAACCTGCCTGAGGGAGAAGCTGTTTTCCAGGTAACATCAGGTGGAATAATGGATGCGAAAGACTGA
Protein Sequence
  • >LOC_Os11g04954.1
    MAPSKQYDEGGQLQLMDAERIEEEEECFESIDKLISQGINSGDVKKLQDAGIYTCNGLMMHTKKSLTGIKGLSEAKVDKICEAAEKLLSQGFMTGSDLLIKRKSVVRITTGSQALDELLGGGIETLCITEAFGEFRSGKTQLAHTLCVSTQLPIHMHGGNGKVAYIDTEGTFRPERIVPIAERFGMDANAVLDNIIYARAYTYEHQYNLLLGLAAKMAEEPFRLLIVDSVIALFRVDFSGRGELAERQQKLAQMLSRLTKIAEEFNVAVYITNQVIADPGGGMFITDPKKPAGGHVLAHAATIRLMLRKGKGEQRVCKIFDAPNLPEGEAVFQVTSGGIMDAKD*