Information report for OsDMC1;DMC1B
Gene Details
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Functional Descriptions
- The OsDMC1-RNAi lines grew normally during their vegetative phase but showed spikelet and pollen sterility.
- The sterility phenotypes were associated with down-regulated OsDMC1 transcript and protein levels mediated by RNAi.
- OsDMC1 was expressed at high-levels in reproductive organs, low-levels in roots, and undetectable levels in leaves and seedlings.
- Isolation and characterization of OsDMC1 , the rice homologue of the yeast DMC1 gene essential for meiosis.
- Further cytological observations of male meiocytes revealed that knock-down of OsDMC1 led to defects in bivalent formation and subsequent unequal chromosome segregation and irregular spore generation, and induced changes in male meiotic progression.
- DNA binding and pairing activities of Oryza sativa disrupted meiotic cDNA1 (OsDMC1) from rice have been reported earlier.
- A cloned cDNA corresponding to OsDMC1 from rice anther tissue was expressed in Escherichia coli.
- Here, we analyzed the function of OsDMC1 in meiosis using an RNA interference approach.
- These data indicate that OsDMC1 is essential for rice meiosis and plays an important role in homologous pairing.
- These biochemical differences between the DMC1A and DMC1B proteins may provide important insight into their functional differences during meiosis in rice.
- Moreover, OsDMC1 was not detected in pairing-defective mutants, such as pair2, pair3, Oscom1 and Osrad51c, while it was loaded onto meiotic chromosomes in zep1, Osmer3, Oszip4 and Oshei10.
- The reduced number of bivalents and abnormal OsHEI10 foci in Osdmc1a OsDMC1B establishes an essential role for OsDMC1 in crossover formation.
- These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination.
- In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and in vitro OsBRCA2 directly interacts with OsRAD51 and OsDMC1.
- In the homologous recombination pathway, the detailed mechanisms of how OsRAD51 and OsDMC1 work in rice meiosis remain to be explored.
- In addition, both OsRAD51 and OsDMC1 can interact with OsFIGL1 and OsBRCA2, two important components in rice meiosis.
Functional Keywords
- sterility , root , pollen , spikelet , meiosis , reproductive , meiotic , vegetative , anther , seedling , crossover , homologous-recombination , recombination
Literature and News
- Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis . DOI: 10.1007/s004970100065
- DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis . DOI: 10.1111/j.1742-4658.2006.05170.x ; PMID: 16689935
- DNA binding and pairing activity of OsDmc1, a recombinase from rice . DOI: 10.1007/s11103-004-5828-x ; PMID: 15821864
- OsDMC1 is required for homologous pairing in Oryza sativa . DOI: 10.1007/s11103-007-9195-2 ; PMID: 17562186
- Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins . DOI: 10.1093/nar/gkn405 ; PMID: 18583359
- OsDMC1 Is Not Required for Homologous Pairing in Rice Meiosis . DOI: 10.1104/pp.16.00167 ; PMID: 26960731
- Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells . DOI: 10.3389/fpls.2020.600820 ; PMID: 33304374
- OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis . DOI: 10.3390/ijms23179906 ; PMID: 36077304
Gene Resources
- UniProt: Q7GBF7
- EMBL: AB046620, AB064544, AB065112
- AlphaFoldDB: Q7GBF7
- EnsemblPlants: Os11t0146800-01
- Gramene: Os11t0146800-01
- KEGG: dosa:Os11g0146800
- Orthologous matrix: ANPMKPV
- InterPro: IPR010995, IPR011940, IPR013632
- PANTHER: PTHR22942, PTHR22942:SF30
- SUPFAM: SSF47794, SSF52540
- PROSITE: PS50162, PS50163
- Gene3D: 1.10.150.20, 3.40.50.300
- OrthoDB: Q7GBF7
- SWISS-MODEL: Q7GBF7
- Conserved Domain Database: cd19514
- eggNOG: KOG1434
Sequences
cDNA Sequence
- >LOC_Os11g04954.1
AACGCAACGGCACAAGCACAACACCACTTCGAGTGTGTTTGTCGTCTCGCCTCTGCTGCTTCCTTCTCCTACAAGCCGCCGCTCCGACCTCGCCGTCGCCGGTCGCCGGCCGCCTCCGCCTACAGGTGCGGGGGGTCTGTGTGAGCAGATGGCGCCGTCCAAGCAGTACGACGAAGGCGGGCAGCTCCAGCTCATGGATGCAGAGAGGATCGAGGAGGAGGAGGAGTGCTTCGAGTCCATCGACAAGTTAATCTCGCAAGGGATAAACTCTGGAGATGTGAAGAAGCTGCAGGATGCTGGTATCTACACTTGCAATGGCCTCATGATGCATACAAAGAAGAGCCTGACAGGAATCAAGGGATTATCTGAAGCAAAGGTAGACAAGATCTGTGAAGCAGCTGAAAAGCTTCTGAGCCAGGGCTTCATGACAGGAAGTGATCTCCTTATCAAGCGGAAGTCTGTTGTTCGGATCACCACTGGGAGCCAGGCACTTGATGAGCTGCTTGGCGGAGGGATTGAAACACTCTGCATCACAGAGGCATTTGGAGAGTTCCGATCAGGGAAGACCCAGTTGGCTCACACTCTATGTGTCTCCACTCAGCTTCCAATTCACATGCATGGGGGGAACGGGAAGGTTGCTTACATTGACACTGAGGGAACATTCCGGCCTGAACGAATTGTGCCAATTGCTGAGAGATTTGGGATGGATGCCAATGCTGTTCTTGATAACATCATATATGCTCGTGCATACACCTATGAGCACCAGTACAACTTGCTCCTTGGCCTCGCTGCCAAGATGGCTGAAGAGCCTTTCAGGCTTCTGATTGTGGATTCTGTGATTGCACTATTCCGTGTTGATTTCAGTGGCAGGGGTGAACTTGCAGAGCGCCAGCAAAAATTGGCACAGATGCTCTCCCGCCTTACTAAGATAGCTGAGGAGTTCAATGTTGCAGTGTACATCACCAACCAAGTGATTGCGGACCCAGGTGGTGGAATGTTCATAACTGACCCGAAGAAACCTGCGGGAGGCCACGTTCTGGCGCATGCAGCCACCATCCGGTTGATGCTGAGGAAAGGCAAAGGAGAGCAGCGTGTCTGCAAGATCTTTGATGCCCCTAACCTGCCTGAGGGAGAAGCTGTTTTCCAGGTAACATCAGGTGGAATAATGGATGCGAAAGACTGAATGTTCACTGAGGGTGCTTCCTGTGTTCAAATTGTTCAGGTTTCTCCAAGTAAATTTCTGCATAGGCCATATAGGTTAGGTACATTGATAAAAGAATACAACTACTGCTGCTGCTATGTTTTTGCCACTGCTGAGGAAAGAGGCAACCTCATTTATCAAGACGGTTATATCTCACATTGGGTATTATGAGTTATGTGATCAACAGTTGTACTGAAACAAGTGCTAGTATTGTCTTAAGTGAAAATATAAGAGGTTAACATGTTGGTTTCCCCAGATACTATTTTTTGTACCTGCTCGTGCACAATTTATACAATCGGTCTTAAACTGCTGT
CDS Sequence
- >LOC_Os11g04954.1
ATGGCGCCGTCCAAGCAGTACGACGAAGGCGGGCAGCTCCAGCTCATGGATGCAGAGAGGATCGAGGAGGAGGAGGAGTGCTTCGAGTCCATCGACAAGTTAATCTCGCAAGGGATAAACTCTGGAGATGTGAAGAAGCTGCAGGATGCTGGTATCTACACTTGCAATGGCCTCATGATGCATACAAAGAAGAGCCTGACAGGAATCAAGGGATTATCTGAAGCAAAGGTAGACAAGATCTGTGAAGCAGCTGAAAAGCTTCTGAGCCAGGGCTTCATGACAGGAAGTGATCTCCTTATCAAGCGGAAGTCTGTTGTTCGGATCACCACTGGGAGCCAGGCACTTGATGAGCTGCTTGGCGGAGGGATTGAAACACTCTGCATCACAGAGGCATTTGGAGAGTTCCGATCAGGGAAGACCCAGTTGGCTCACACTCTATGTGTCTCCACTCAGCTTCCAATTCACATGCATGGGGGGAACGGGAAGGTTGCTTACATTGACACTGAGGGAACATTCCGGCCTGAACGAATTGTGCCAATTGCTGAGAGATTTGGGATGGATGCCAATGCTGTTCTTGATAACATCATATATGCTCGTGCATACACCTATGAGCACCAGTACAACTTGCTCCTTGGCCTCGCTGCCAAGATGGCTGAAGAGCCTTTCAGGCTTCTGATTGTGGATTCTGTGATTGCACTATTCCGTGTTGATTTCAGTGGCAGGGGTGAACTTGCAGAGCGCCAGCAAAAATTGGCACAGATGCTCTCCCGCCTTACTAAGATAGCTGAGGAGTTCAATGTTGCAGTGTACATCACCAACCAAGTGATTGCGGACCCAGGTGGTGGAATGTTCATAACTGACCCGAAGAAACCTGCGGGAGGCCACGTTCTGGCGCATGCAGCCACCATCCGGTTGATGCTGAGGAAAGGCAAAGGAGAGCAGCGTGTCTGCAAGATCTTTGATGCCCCTAACCTGCCTGAGGGAGAAGCTGTTTTCCAGGTAACATCAGGTGGAATAATGGATGCGAAAGACTGA
Protein Sequence
- >LOC_Os11g04954.1
MAPSKQYDEGGQLQLMDAERIEEEEECFESIDKLISQGINSGDVKKLQDAGIYTCNGLMMHTKKSLTGIKGLSEAKVDKICEAAEKLLSQGFMTGSDLLIKRKSVVRITTGSQALDELLGGGIETLCITEAFGEFRSGKTQLAHTLCVSTQLPIHMHGGNGKVAYIDTEGTFRPERIVPIAERFGMDANAVLDNIIYARAYTYEHQYNLLLGLAAKMAEEPFRLLIVDSVIALFRVDFSGRGELAERQQKLAQMLSRLTKIAEEFNVAVYITNQVIADPGGGMFITDPKKPAGGHVLAHAATIRLMLRKGKGEQRVCKIFDAPNLPEGEAVFQVTSGGIMDAKD*