Gene Details:

Functional Descriptions:

  • These expression profiles suggested that overexpression of OsNMD3(deltaNLS) affected ribosome biogenesis and certain basic pathways, leading to pleiotropic abnormalities in plant growth.
  • Analyses of the transactivation activity and cellulose biosynthesis level revealed low protein synthesis efficiency in the transgenic plants compared with the wild-type plants.
  • A dominant negative form of OsNMD3 with a truncated nuclear localization sequence (OsNMD3(deltaNLS)) was retained in the cytoplasm, consequently interfering with the release of OsNMD3 from pre-60S particles and disturbing the assembly of ribosome subunits.
  • Moreover, global expression profiles of the wild-type and transgenic plants were investigated using the Illumina RNA sequencing approach.
  • Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice.
  • sativa NMD3 (OsNMD3) shares all the common motifs and shuttles between the nucleus and cytoplasm via CRM1/XPO1.
  • This study chose a highly conserved trans-factor, the 60S ribosomal subunit nuclear export adaptor NMD3, to characterize the mechanism of ribosome biogenesis in the monocot plant Oryza sativa (rice).

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os10g42320.1
    GCGCCGCCGCCGTCTCTTTTCACCTACAAAAACCCTAAGCCCAAAAATCTCCATCCCCCCCGCCCCCAGAGATCCCCCAATCCACTCGATTCGATCCAATCCGGCGAGGTACAGGATGCTGCCGGGGTCGGCGGCGCCGGCGGCTGGGAGCGGCATGTTCGTGCCAACGCAGACGGTGGGCACGGTGCTGTGCTGCATCTGCGGCGTGGCGATGCAGCCCAACCCGGCCAACATGTGCGCGCGCTGCATCCGCGCCCGCGTCGACATCACCGAGGGCGTCCCGCGCCACGCCGCCGTCGTCTACTGCCCCGACTGCACCTCCTACCTCCAGCCGCCGCGCTCCTGGCTCCGCGCCGGCCCGGAGTCCCCCGAGCTCATGCAGATCCTCCTCCGCCGCCTCAACCGCCCCCTCGCCCGCCTCCGCGTCTCCCTCTCCGCCGCCGAGTTCGTCTTCTCCGAGCCCCACTCCAAGCGCCTCCGCCTCAAGCTCCGCCTCCGCCGCGAGGTCTTCAACGCCGTCGTCCTCGAGCAGACCCACCCCGTCGAGTTCACCGTCCACGATCGCCTCTGCGATGCCTGCGCCCGCGCCCAGTCCAACCCCGACCAGTGGGTCGCCGTCGTCCAGCTCCGCCAGCACGTCCCGCACCGCCGCACCTTCCTCTACCTCGAGCAGCTCCTCCTCAAGCACGGCCAGGCCTCCCTCGCCCTCCGCGTCGCCGCCGCCCCCGGCGGCCTCGACTTCTTCTTCGGCTCCCGCTCGCACGCCGCCCGCCTCGTCGACTTCCTCGCCACCGTCGCCCCCGTCCAGACCCAGACCGCCAAGCAGCTCGTCTCCCACGACACCAAGAGCAACGTCTACAACTACAAGCACACCTTCTCCGTCGAGATCTGCCCCATCTGCCGGGAGGACCTCATCGCCCTTAGCCCTCAGGTGTCCCGCGATCTTGGAGGCCTTGGACCTATCGTCCTCTGCATCAAGGTCACCAATGCCATCGCTCTGCTTGACCCCCTCACCCTGCGTGTGCACCACCTCGAGGAAAAGAAGTACAGGGTCTACAACTTCAAGGCTGCTCTCACCAGCAAGCAGCTCGTCGAGTACATCGTGCTAGACATCGAGCACGAGTCACCAGAGATTACCATTGACGGATCTCGCTACCAGTTGGCTTATGCCCAGGTCGCCCGGGTCTCCGACTTTGGGAAAAACGACACCATGTTCACCGTGCGGACTCATCTTGGCCACCTGCTGAATCCTGGAGACCTTGCCCTTGGGTATGATCTTTATGGTGCCAACTTGAATGATGATGACATGGACACAGCTATGATGCGCCACAGTCTGCCTGAAGTCATTCTTGTTAAGAAGAGCTTCGCCAAGAGGCCACGCACACGCCGATGGAAGCTGAAGAGGCTTCCAATGGAGGAAGATGCTGTAAATAAGGCCAAGGGGGAGCTAGAGAAGAGGGCTGATGAGTATGAGGAGTTTCTCAGGGACTTGGAGCACAACTCTGATATTAGATTCCAGATTGATCTATACAAGGATGAGGACTACAGGTCGGAGATGGCATCTACTATTGGTGATGATGTTCCCACTGTGCCGATTGAGGAACTAATTGAGGACCTAAGTCTTGGTGATGATGAGGAGGAGGATGAAGGTGGGGAAGGCAACCAACATGCTGTTATGGTGGGATGAGTTAATATGTTTACCTGTGCTGTGCTTCTGCCTTCTGGTGATGGTTGCTGCCAACCGCAGATCATAGGTCTGAACTGCATCTGTTTAATCCGTATAAGTTTTGTTCTCCCCTTTACTTCTATAAGTTATAAGTTTTGTTGCTAAATTGTTTCAACTTTTGCTGTTTCTCATGATAGATGTTGTATCACCTTATCCTGTATCTGGTGTTGCAGCTTGGCTTAAATTATATTTCTCTATTCTTATTGTCTGTGATATGTTGTTTTCTTGCCATAGTGTGGCTGATCACAACATATCTTAAAATAGATGCATTGTTTGATTTCCAAAACATCTAGTTTGGTGTTTAGATTGTGCCAGATTCATATTTTTACTATATGTTTTTAGTCCCCATTATGTGGTTTGCAAGCGTGGAATATGATTGTGGATTTATGCTGAGTCATGACTAGAGAATTGTAGGTATGACCTGAAAACGTCTGCCATGTTGAGTGTCACTCCAATAAATTGATTAAAAAACTAATAAAGATAAGTGTAGTATAATATCATACCCAGGAGCCATGATGAGACAATAATGTGCATTTTTAGTGATTGCAATTCAACTGCTGCTGTTTTATCAGGCACTTCATTCAGACTATGCCTACATCGTGCAATAAGAGGTTAGATATGTAGAGTTGATGGACAGAACAGATGGTGCTCCACTAATAAGCGATGAGTACTGGTTTGGTAAAACTTTAATAAGTTAAGAATTTTACGGATCTTTTTTTTCTATTACTTTCACAAGGTGGTGCTGTTCTCAAACCTAATGACTTATAATCGT
CDS Sequence
  • >LOC_Os10g42320.1
    ATGCTGCCGGGGTCGGCGGCGCCGGCGGCTGGGAGCGGCATGTTCGTGCCAACGCAGACGGTGGGCACGGTGCTGTGCTGCATCTGCGGCGTGGCGATGCAGCCCAACCCGGCCAACATGTGCGCGCGCTGCATCCGCGCCCGCGTCGACATCACCGAGGGCGTCCCGCGCCACGCCGCCGTCGTCTACTGCCCCGACTGCACCTCCTACCTCCAGCCGCCGCGCTCCTGGCTCCGCGCCGGCCCGGAGTCCCCCGAGCTCATGCAGATCCTCCTCCGCCGCCTCAACCGCCCCCTCGCCCGCCTCCGCGTCTCCCTCTCCGCCGCCGAGTTCGTCTTCTCCGAGCCCCACTCCAAGCGCCTCCGCCTCAAGCTCCGCCTCCGCCGCGAGGTCTTCAACGCCGTCGTCCTCGAGCAGACCCACCCCGTCGAGTTCACCGTCCACGATCGCCTCTGCGATGCCTGCGCCCGCGCCCAGTCCAACCCCGACCAGTGGGTCGCCGTCGTCCAGCTCCGCCAGCACGTCCCGCACCGCCGCACCTTCCTCTACCTCGAGCAGCTCCTCCTCAAGCACGGCCAGGCCTCCCTCGCCCTCCGCGTCGCCGCCGCCCCCGGCGGCCTCGACTTCTTCTTCGGCTCCCGCTCGCACGCCGCCCGCCTCGTCGACTTCCTCGCCACCGTCGCCCCCGTCCAGACCCAGACCGCCAAGCAGCTCGTCTCCCACGACACCAAGAGCAACGTCTACAACTACAAGCACACCTTCTCCGTCGAGATCTGCCCCATCTGCCGGGAGGACCTCATCGCCCTTAGCCCTCAGGTGTCCCGCGATCTTGGAGGCCTTGGACCTATCGTCCTCTGCATCAAGGTCACCAATGCCATCGCTCTGCTTGACCCCCTCACCCTGCGTGTGCACCACCTCGAGGAAAAGAAGTACAGGGTCTACAACTTCAAGGCTGCTCTCACCAGCAAGCAGCTCGTCGAGTACATCGTGCTAGACATCGAGCACGAGTCACCAGAGATTACCATTGACGGATCTCGCTACCAGTTGGCTTATGCCCAGGTCGCCCGGGTCTCCGACTTTGGGAAAAACGACACCATGTTCACCGTGCGGACTCATCTTGGCCACCTGCTGAATCCTGGAGACCTTGCCCTTGGGTATGATCTTTATGGTGCCAACTTGAATGATGATGACATGGACACAGCTATGATGCGCCACAGTCTGCCTGAAGTCATTCTTGTTAAGAAGAGCTTCGCCAAGAGGCCACGCACACGCCGATGGAAGCTGAAGAGGCTTCCAATGGAGGAAGATGCTGTAAATAAGGCCAAGGGGGAGCTAGAGAAGAGGGCTGATGAGTATGAGGAGTTTCTCAGGGACTTGGAGCACAACTCTGATATTAGATTCCAGATTGATCTATACAAGGATGAGGACTACAGGTCGGAGATGGCATCTACTATTGGTGATGATGTTCCCACTGTGCCGATTGAGGAACTAATTGAGGACCTAAGTCTTGGTGATGATGAGGAGGAGGATGAAGGTGGGGAAGGCAACCAACATGCTGTTATGGTGGGATGA
Protein Sequence
  • >LOC_Os10g42320.1
    MLPGSAAPAAGSGMFVPTQTVGTVLCCICGVAMQPNPANMCARCIRARVDITEGVPRHAAVVYCPDCTSYLQPPRSWLRAGPESPELMQILLRRLNRPLARLRVSLSAAEFVFSEPHSKRLRLKLRLRREVFNAVVLEQTHPVEFTVHDRLCDACARAQSNPDQWVAVVQLRQHVPHRRTFLYLEQLLLKHGQASLALRVAAAPGGLDFFFGSRSHAARLVDFLATVAPVQTQTAKQLVSHDTKSNVYNYKHTFSVEICPICREDLIALSPQVSRDLGGLGPIVLCIKVTNAIALLDPLTLRVHHLEEKKYRVYNFKAALTSKQLVEYIVLDIEHESPEITIDGSRYQLAYAQVARVSDFGKNDTMFTVRTHLGHLLNPGDLALGYDLYGANLNDDDMDTAMMRHSLPEVILVKKSFAKRPRTRRWKLKRLPMEEDAVNKAKGELEKRADEYEEFLRDLEHNSDIRFQIDLYKDEDYRSEMASTIGDDVPTVPIEELIEDLSLGDDEEEDEGGEGNQHAVMVG*