Gene Details:

• MSU gene ID: LOC_Os10g41310
• RAPdb gene ID: Os10g0562700
• Gene Symbol: REL2 OsDLT10 RSD1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• In this study, we identified and characterized a rolling and erect leaf mutant in rice and named it as REL2.
• The results from quantitative RT-PCR analysis indicated that REL2 functioning in the leaf shape formation might have functional linkage with many genes associated with the bulliform cells development, auxin synthesis and transport, etc.
• REL2 is the DUF domains contained protein which involves in the control of leaf rolling in rice.
• We firstly mapped REL2 to a 35-kb physical region of chromosome 10 by map-based cloning strategy.
• Further studies showed that REL2 protein is mainly distributed along the plasma membrane of cells and the REL2 gene is relatively higher expressed in younger leaves of rice.
• Here, we report a semi-dwarf and low tillering mutant OsDLT10 (dwarf and low tillering 10) that exhibited reduced tiller number, semi-dwarfism, increased grain width, low seed-setting rate, curled leaf tip and a series of abnormalities of agronomic traits.
• Expression pattern analysis indicated that OsDLT10 was primarily expressed in the stem node, the basic part of axillary bud and leaf sheath, pulvinus.
• Phenotypic observations showed that OsDLT10 mutants had defects in tiller bud formation and grew slowly at the tillering stage.
• Endogenous auxin content decreased significantly at the base of stem node and axillary bud in OsDLT10 mutants.
• The hormone treatment investigation indicated that extremely high of exogenous auxin concentrations can inhibit the expression of OsDLT10.
• The results showed that OsDLT10 was related to auxin.
• These results revealed that OsDLT10 played a critical role in influencing tiller number, likely in association with hormone signals and the WUS-CLV pathway, to regulate axillary bud development in rice.
• This study characterized the functions of RSD1 and OsSDD1 in rice stomatal development.
• OsSDD1 and RSD1 are both required for inhibiting ectopic asymmetric cell divisions (ACDs) and clustered stomata.
• By dehydration stress assay, the decreased stomatal density of RSD1 mutants enhanced their dehydration avoidance.
• RSD1 Is Essential for Stomatal Patterning and Files in Rice.
• It was found that the deletion of RSD1 would lead to the disorder of gene expression regarding stomatal development, especially the expression of stomatal density and distribution 1 (OsSDD1).
• Through the construction of OsSDD1 deletion mutants by CRISPR-Cas9, we found that, similar to RSD1 mutants, the ossdd1 mutants have clustered stomata and extra small cells adjacent to the stomata.

Function-related keywords:

• leaf ,  map-based-cloning ,  auxin ,  leaf-shape ,  erect ,  plasma-membrane ,  leaf-rolling ,  tillering ,  stem ,  development ,  grain ,  tiller ,  tiller-number ,  grain-width ,  node ,  cell-division ,  stress ,  stomatal ,  stomata ,  stomatal-development

Literature:

• REL2, A Gene Encoding An Unknown Function Protein which Contains DUF630 and DUF632 Domains Controls Leaf Rolling in Rice .  DOI:  10.1186/s12284-016-0105-6 ;  PMID:  27473144
• Rice dwarf and low tillering 10 (OsDLT10) regulates tiller number by monitoring auxin homeostasis .  DOI:  10.1016/j.plantsci.2020.110502 ;  PMID:  32563453
• Characterization of dwarf and narrow leaf (dnl-4) mutant in rice .  DOI:  10.1080/15592324.2020.1849490 ;  PMID:  33300429
• RSD1 Is Essential for Stomatal Patterning and Files in Rice .  DOI:  10.3389/fpls.2020.600021 ;  PMID:  33329664

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: