Gene Details:
- MSU gene ID: LOC_Os10g41260
- RAPdb gene ID: Os10g0562100
- Gene Symbol: OsMYBS2
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Transient expression assays with barley half-seeds showed that OsMYBS1 and OsMYBS2 transactivate a promoter containing the TATCCA element when sugar is provided, whereas OsMYBS3 represses transcription of the same promoter under sugar starvation.
- GF14A protein affects the repression activity of OsMYBS2 by regulating its nucleocytoplasmic shuttling, and Ser53 is necessary for OsMYBS2 to be retained in the cytoplasm.
- The OsMYBS2 transcription factor was shown recently to reduce activation of the αAmy3 promoter by competing for the binding site of the TA box of the αAmy3 promoter with the potent OsMYBS1 activator.
- CONCLUSIONS: Our results demonstrate that knockdown of the transcription factor gene OsMYBS2 increased the activity of the αAmy3 promoter and improved the yield of recombinant proteins secreted in rice cell suspension cultures.
Function-related keywords:
Literature:
- Three novel MYB proteins with one DNA binding repeat mediate sugar and hormone regulation of alpha-amylase gene expression . DOI: 10.1105/tpc.001735 ; PMID: 12172034
- The coordination of OsbZIP72 and OsMYBS2 with reverse roles regulates the transcription of OsPsbS1 in rice . DOI: 10.1111/nph.16877 ; PMID: 33411361
- Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells . DOI: 10.1186/s13007-021-00799-2 ; PMID: 34560901
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os10g41260.1
AGTAGTCTCCTCCAAACCGTCGAGAGCCCAACAATAATAATCCCCCCTTTTCATCGCGGCGACGATACCCGCCATTTTCATCTCATTCTCACCACCAAAAGCGACCACGCAGCGACCGGCCACCGATCGAGATGGAGCAGCATGAGGAGGCAGCGGAGAGGAAGCCTTCGCCGCCGGTGATATTCCGGCTGTTCGGCGTCGAGGTCCGCGGCGGCGGCGGCGGAGTTGACGAGGAGGAGTACGAGGAGGAGGAGGTGGAGGGTGGATTGTTCATCAAGAAGAGCTCCAGTATGCCCAACCTCACCTCCATCGACCCGCTGCCGGTGCCGGCCGACGGCGGCAAACGGCGCGCCTCCGACGACTCCGAGCTCGCCTCCGGCCAGCAGAAGCGCCGCCGCCGCAAGGTGCAGGAGAGGAAGAAAGGGGTACCATGGACTGAGGAGGAGCACAAGAAATTCCTGGAAGGGCTGAGGCAGCTGGGGAAAGGGGACTGGAGAGGCATCTCCAAGAACTTTGTGACCAGCAGGACGGCGACTCAGGTGGCCAGCCACGCCCAGAAGTACTTCCTCCGGCAGACCAACCCTGGCAAAAAGAAGCGCCGGGCCAGCCTCTTTGATGTTGTTGCTGAGTGCAGTGATGATCAGCTTCCAAGTCCTCAGAGTGTTGGAACTAAGCCTCCTACCCAGGATATAATTCATACAGATCGCGGCGATGTCCCGATACTAAGCTATCCAGTTGCTAGAGGCTTTAGAGGCGATAGCGTGCAGGTTGATGAACTAACTGAATATGTGAAGAGATTAAAGGCCGCCGAGGACATGTCGCTCTCCATGATCTCTGGACTGGAAATGGCATCATCATCCATCAGCAGTCTAGAGCTCAGTATCGCGCCTCCTCATTGCGCGATCGAGGCGGCCATCAAGGTGCTGTGATCCAGACGCAATTCTCCACCGAAGGAATCTGGATCGGCTTCAGCTACTGTTCTTCGTCGCCGCTGTTGTTGTTTGTTGTTGTTGTTTTTTTTTTTTTTTTGCGGGGGTTGTTTGTTGTTGTTGTTGTTGTAGTTGTCATGCTAACTTTGTATTTGGGTCATGTGGCGTTTCTTTCAGCAGTCTTATATAATACAGTGAGAATGTCAGTACCTTCCGAGACATGTTTAATTAAGCCTTGGTTGGTTGGTATAAGTTGGTGAAAAGAAAACCAGATAGTAGCCTGTCTGTATTGTACGGTTGCAAAATTGGAGTATATTGATGCAGAGATCATGAAGGGTCTCCATCTTGTTCATAAGTCTAGTTTGTTTCCTGTTCCTGTCACAGGAGGCAGCTGGGACATCTTCTTGTGTGGCTCTGAGCTTCTTCCAGTGCTTCAATAATTGGACCCTTCCCCTTCATGTCCTTGTGACCATTGGTCCCAGCCA
CDS Sequence
- >LOC_Os10g41260.1
ATGGAGCAGCATGAGGAGGCAGCGGAGAGGAAGCCTTCGCCGCCGGTGATATTCCGGCTGTTCGGCGTCGAGGTCCGCGGCGGCGGCGGCGGAGTTGACGAGGAGGAGTACGAGGAGGAGGAGGTGGAGGGTGGATTGTTCATCAAGAAGAGCTCCAGTATGCCCAACCTCACCTCCATCGACCCGCTGCCGGTGCCGGCCGACGGCGGCAAACGGCGCGCCTCCGACGACTCCGAGCTCGCCTCCGGCCAGCAGAAGCGCCGCCGCCGCAAGGTGCAGGAGAGGAAGAAAGGGGTACCATGGACTGAGGAGGAGCACAAGAAATTCCTGGAAGGGCTGAGGCAGCTGGGGAAAGGGGACTGGAGAGGCATCTCCAAGAACTTTGTGACCAGCAGGACGGCGACTCAGGTGGCCAGCCACGCCCAGAAGTACTTCCTCCGGCAGACCAACCCTGGCAAAAAGAAGCGCCGGGCCAGCCTCTTTGATGTTGTTGCTGAGTGCAGTGATGATCAGCTTCCAAGTCCTCAGAGTGTTGGAACTAAGCCTCCTACCCAGGATATAATTCATACAGATCGCGGCGATGTCCCGATACTAAGCTATCCAGTTGCTAGAGGCTTTAGAGGCGATAGCGTGCAGGTTGATGAACTAACTGAATATGTGAAGAGATTAAAGGCCGCCGAGGACATGTCGCTCTCCATGATCTCTGGACTGGAAATGGCATCATCATCCATCAGCAGTCTAGAGCTCAGTATCGCGCCTCCTCATTGCGCGATCGAGGCGGCCATCAAGGTGCTGTGA
Protein Sequence
- >LOC_Os10g41260.1
MEQHEEAAERKPSPPVIFRLFGVEVRGGGGGVDEEEYEEEEVEGGLFIKKSSSMPNLTSIDPLPVPADGGKRRASDDSELASGQQKRRRRKVQERKKGVPWTEEEHKKFLEGLRQLGKGDWRGISKNFVTSRTATQVASHAQKYFLRQTNPGKKKRRASLFDVVAECSDDQLPSPQSVGTKPPTQDIIHTDRGDVPILSYPVARGFRGDSVQVDELTEYVKRLKAAEDMSLSMISGLEMASSSISSLELSIAPPHCAIEAAIKVL*