Gene Details:

• MSU gene ID: LOC_Os10g35436
• RAPdb gene ID: Os10g0497300
• Gene Symbol: Rf1a Rf5
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Two fertility restorer genes, Rf1a and Rf1b, were identified at the classical locus Rf-1 as members of a multigene cluster that encode pentatricopeptide repeat proteins.
• Rf1a and RF1B are both targeted to mitochondria and can restore male fertility by blocking ORF79 production via endonucleolytic cleavage (Rf1a) or degradation (RF1B) of dicistronic B-atp6/orf79 mRNA.
• In this study, we cloned the restorer gene Rf5 for Hong-Lian CMS in rice and studied its fertility restoration mechanism with respect to the processing of the CMS-associated transcript atp6-orfH79.
• Furthermore, we found that Rf5 and GRP162 are both components of a restoration of fertility complex (RFC) that is 400 to 500 kD in size and can cleave CMS-associated transcripts in vitro.
• The rice pentatricopeptide repeat protein Rf5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162.
• The hybrid F(1) plants containing one restorer gene, either Rf5 or Rf6, displayed 50% normal pollen grains with I(2)-KI solution; however, those with both Rf5 and Rf6 displayed 75% normal pollens.

Function-related keywords:

• fertility ,  mitochondria ,  grain ,  pollen ,  sterile

Literature:

• Mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice using microsatellite markers .  DOI:  10.1007/BF02884944
• Positional cloning of the rice Rf-1 gene, a restorer of BT-type cytoplasmic male sterility that encodes a mitochondria-targeting PPR protein .  DOI:  10.1007/s00122-004-1591-2 ;  PMID:  14968308
• Two non-allelic nuclear genes restore fertility in a gametophytic pattern and enhance abiotic stress tolerance in the hybrid rice plant .  DOI:  10.1007/s00122-011-1755-9 ;  PMID:  22139140
• A rapid PCR-aided selection of a rice line containing the Rf-1 gene which is involved in restoration of the cytoplasmic male sterility .  DOI:  10.1023/A:1009601007175
• The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162 .  DOI:  10.1105/tpc.111.093211 ;  PMID:  22247252
• A codominant DNA marker closely linked to the rice nuclear restorer gene, RF-1, identified with inter-SSR fingerprinting .  DOI:  10.1139/g96-152 ;  PMID:  8983188
• Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing .  DOI:  10.1105/tpc.105.038240 ;  PMID:  16489123

Related News:

Gene Resources:

• NCBI ID: AB110443  , AB110444
• UniProt accessions:

Sequences: