Gene Details:
- MSU gene ID: LOC_Os10g34360
- RAPdb gene ID: Os10g0484800
- Gene Symbol: OsPKS1 YY2 OsLAP6
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Expression of YY2 mRNA was confined to the tapetal cells.
- Two cDNAs (YY1 and YY2), representing genes that are specifically expressed in anthers at the uninucleate microspore stage, were isolated and characterized.
- We identified a rice mutant OsLAP6 with complete male sterile phenotype caused by defects in pollen exine formation.
- Cytological observations revealed that abnormal bacula deposition and ubisch body structures for sexine formation led to pollen rupture in OsPKS1.
- The expression analysis showed that the OsPKS1 was highly expressed in tapetal cells and anther locules from stage 9 to stage 11 during anther development in rice.
- The OsPKS1 knockout mutants obtained by Crispr-Cas9-mediated editing exhibited a complete male sterile phenotype.
Function-related keywords:
- tapetal , microspore , anther , pollen , sterile , pollen-exine-formation , development , anther-development
Literature:
- Isolation and characterization of two cDNA clones for mRNAs that are abundantly expressed in immature anthers of rice (Oryza sativa L.) . DOI: 10.1007/BF00019551 ; PMID: 8704128
- Tapetum degeneration retardation is critical for aliphatic metabolism and gene regulation during rice pollen development . DOI: 10.1093/mp/ssn028 ; PMID: 19825565
- Conserved metabolic steps for sporopollenin precursor formation in tobacco and rice . DOI: 10.1111/ppl.12018 ; PMID: 23231646
- OsLAP6/OsPKS1, an orthologue of Arabidopsis PKSA/LAP6, is critical for proper pollen exine formation . DOI: 10.1186/s12284-017-0191-0 ; PMID: 29282604
- OsPKS1 is required for sexine layer formation, which shows functional conservation between rice and Arabidopsis . DOI: 10.1016/j.plantsci.2018.08.009 ; PMID: 30466580
- OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice . DOI: 10.1186/s12284-020-00451-y ; PMID: 33409767
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os10g34360.1
TTGCACTGTCACACAGCGAAATCACAGGACTAGAATACTTGCTGACTACTGAGGCGAAAGGATACCGGGTGGCGAAGATGGCTGACCTTGGATTCGGCGATGCCAGGAGTGGCAATGGCAGCAGGAGCCAATGCTCCAGGGGGAAGGCGATGCTGCTCGCCCTCGGCAAGGGCCTCCCTGAGCAAGTTCTTCCCCAGGAGAAGGTCGTCGAGACCTACCTCCAGGACACCATCTGCGACGATCCTGCAACAAGGGCAAAGCTGGAAAGACTTTGCAAGACCACCACAGTGAGGACAAGGTACACTGTCATGTCAAAGGAGCTCCTAGACGAGCACCCAGAGCTCAGGACTGAGGGAACTCCAACACTGACGCCACGGCTTGACATCTGCAATGCTGCAGTGCTTGAGCTTGGTGCTACTGCAGCCCGTGCCGCCCTTGGTGAATGGGGGCGTCCAGCAGCTGACATCACCCACCTTGTCTACATCTCGTCCAGTGAGCTTCGCCTCCCAGGGGGTGACCTTTTCCTGGCAACTCGCCTTGGCCTCCATCCAAACACCGTCCGCACTTCCCTTCTCTTCCTTGGCTGCTCCGGTGGCGCTGCCGCGCTCCGCACCGCCAAGGACATTGCTGAGAACAACCCAGGGAGCCGCGTCCTTGTAGTAGCCGCGGAGACGACGGTGCTGGGATTCCGGCCACCAAGTCCTGACCGTCCTTACGATCTTGTTGGTGCTGCCCTGTTTGGTGACGGCGCATCAGCTGCGATCATTGGAGCAGGCCCCATTGCTGCTGAGGAGAGTCCCTTCCTAGAGCTTCAGTTCTCAACACAGGAGTTCCTACCAGGGACGGACAAGGTAATTGATGGCAAGATCACTGAGGAAGGGATTAATTTCAAACTGGGGCGTGATTTGCCCGAAAAGATTGAAAACCGTATAGAAGGGTTCTGCAGGACACTCATGGATCGGGTTGGGATAAAGGAGTTCAATGATGTATTCTGGGCTGTGCATCCTGGTGGTCCAGCAATACTGAACAGGCTAGAGGTTTGCCTTGAACTCCAGCCAGAGAAGCTCAAGATCAGTAGAAAGGCCCTGATGAACTATGGTAATGTGAGCAGCAACACCGTCTTCTATGTGTTGGAGTATTTGAGGGATGAGTTGAAGAAAGGGATGATAAGGGAAGAATGGGGACTGATCTTGGCTTTTGGCCCAGGCATCACATTTGAAGGAATGCTAGTTCGAGGCATTAACTGAGACTGAAAGGGGTCCAAGAAGACTTTCAGCTAGATGGAAGAATCACAAACATACCATTTCCAGGTTAAGTATATAGTATGAACTTGAAACATCTCATAAGGAGCATAGATGTCTTGACAGCATCTGTGCACTACATAGTTATTGTTTTACTGTAATCTATGTTTTATGTATCAAACAATGTGAGGTACTCTAGTTTGAATGCAAGATACTTGAAAAAATATCAGGCAATACATTGCATCCTGTTACATTGTCATGTATAGTAATTTGCAAGAGTATTTATATGAATTTCTAGTGGCTACACAAATGGCATAACGTGAGAAAAGTAATGTGATAGTCCAACTACCTTCCTCTCAAAAGAATAAATAAGCAGAACAAATATTGTTTAAGA
CDS Sequence
- >LOC_Os10g34360.1
ATGGCTGACCTTGGATTCGGCGATGCCAGGAGTGGCAATGGCAGCAGGAGCCAATGCTCCAGGGGGAAGGCGATGCTGCTCGCCCTCGGCAAGGGCCTCCCTGAGCAAGTTCTTCCCCAGGAGAAGGTCGTCGAGACCTACCTCCAGGACACCATCTGCGACGATCCTGCAACAAGGGCAAAGCTGGAAAGACTTTGCAAGACCACCACAGTGAGGACAAGGTACACTGTCATGTCAAAGGAGCTCCTAGACGAGCACCCAGAGCTCAGGACTGAGGGAACTCCAACACTGACGCCACGGCTTGACATCTGCAATGCTGCAGTGCTTGAGCTTGGTGCTACTGCAGCCCGTGCCGCCCTTGGTGAATGGGGGCGTCCAGCAGCTGACATCACCCACCTTGTCTACATCTCGTCCAGTGAGCTTCGCCTCCCAGGGGGTGACCTTTTCCTGGCAACTCGCCTTGGCCTCCATCCAAACACCGTCCGCACTTCCCTTCTCTTCCTTGGCTGCTCCGGTGGCGCTGCCGCGCTCCGCACCGCCAAGGACATTGCTGAGAACAACCCAGGGAGCCGCGTCCTTGTAGTAGCCGCGGAGACGACGGTGCTGGGATTCCGGCCACCAAGTCCTGACCGTCCTTACGATCTTGTTGGTGCTGCCCTGTTTGGTGACGGCGCATCAGCTGCGATCATTGGAGCAGGCCCCATTGCTGCTGAGGAGAGTCCCTTCCTAGAGCTTCAGTTCTCAACACAGGAGTTCCTACCAGGGACGGACAAGGTAATTGATGGCAAGATCACTGAGGAAGGGATTAATTTCAAACTGGGGCGTGATTTGCCCGAAAAGATTGAAAACCGTATAGAAGGGTTCTGCAGGACACTCATGGATCGGGTTGGGATAAAGGAGTTCAATGATGTATTCTGGGCTGTGCATCCTGGTGGTCCAGCAATACTGAACAGGCTAGAGGTTTGCCTTGAACTCCAGCCAGAGAAGCTCAAGATCAGTAGAAAGGCCCTGATGAACTATGGTAATGTGAGCAGCAACACCGTCTTCTATGTGTTGGAGTATTTGAGGGATGAGTTGAAGAAAGGGATGATAAGGGAAGAATGGGGACTGATCTTGGCTTTTGGCCCAGGCATCACATTTGAAGGAATGCTAGTTCGAGGCATTAACTGA
Protein Sequence
- >LOC_Os10g34360.1
MADLGFGDARSGNGSRSQCSRGKAMLLALGKGLPEQVLPQEKVVETYLQDTICDDPATRAKLERLCKTTTVRTRYTVMSKELLDEHPELRTEGTPTLTPRLDICNAAVLELGATAARAALGEWGRPAADITHLVYISSSELRLPGGDLFLATRLGLHPNTVRTSLLFLGCSGGAAALRTAKDIAENNPGSRVLVVAAETTVLGFRPPSPDRPYDLVGAALFGDGASAAIIGAGPIAAEESPFLELQFSTQEFLPGTDKVIDGKITEEGINFKLGRDLPEKIENRIEGFCRTLMDRVGIKEFNDVFWAVHPGGPAILNRLEVCLELQPEKLKISRKALMNYGNVSSNTVFYVLEYLRDELKKGMIREEWGLILAFGPGITFEGMLVRGIN*