Gene Details:
- MSU gene ID: LOC_Os10g34180
- RAPdb gene ID: Os10g0483000
- Gene Symbol: OsBTF3 Osj10gBTF3
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- The expression of Osj10gBTF3 was primarily constitutive and generally modulated by salt, high temperature and exogenous phytohormone stress.
- Inhibition of a basal transcription factor 3-like gene Osj10gBTF3 in rice results in significant plant miniaturization and typical pollen abortion.
- Our demonstration of the important role of Osj10gBTF3 in rice growth and development provides new insights showing that more complex regulatory functions are associated with BTF3 in plants.
- Knockout of Osj10gBTF3 affects pollen and chloroplast development and significantly reduces the accumulation of fertility-related chloroplast protein OsPPR676.
- Interestingly, the interaction between OsHSP82 and OsPPR676 is only found in the cytoplasm, while the interaction between Osj10gBTF3 and OsPPR676 also occurs inside the chloroplast.
- Further investigation indicates that Osj10gBTF3 enters the chloroplast stroma possibly through the inner chloroplast membrane channel protein Tic110 and then recruits OsCpn60 for the folding or assembly of OsPPR676.
- Our results reveal a chaperone role of Osj10gBTF3 in chloroplast import different from Hsp90 and provide a link between chloroplast transport and pollen development in rice.
Function-related keywords:
- temperature , transcription-factor , salt , phytohormone , pollen , growth , chloroplast , development , chloroplast-development , pollen-development , stroma
Literature:
- Inhibition of a basal transcription factor 3-like gene Osj10gBTF3 in rice results in significant plant miniaturization and typical pollen abortion . DOI: 10.1093/pcp/pcs146 ; PMID: 23147221
- Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice . DOI: 10.3389/fpls.2021.713544 ; PMID: 34421965
Related News:
Gene Resources:
- NCBI ID: AY224525
- UniProt accessions:
Sequences:
cDNA Sequence
- >LOC_Os10g34180.1
AGCCTTACCCCTAGAGCCCTATCCTTTTCCTCTCCCCGAGTCTTCTTCGTCCACTCCCCCTATCCTCTCCTCCCACCGCGCCGCCGCCTCCGCCGCCGCCGCCGCGGCTCCCCCTCCTCCCCACAGCCAAGATGAATGTTGACAAGCTCAAGAAGATGGCAGGTGCTGTGCGCACCGGGGGGAAGGGTAGCGTGCGCAGAAAGAAGAAGGCAGTCCATAAGACTACAACCACAGATGACAAAAGACTTCAGAGCACTCTGAAAAGAGTAGGAGTAAACACCATCCCTGGCATTGAAGAGGTTAACATTTTCAAGGATGATGTGGTTATTCAGTTTCTGAATCCTAAAGTGCAAGCTTCAATTGGTGCTAATACATGGGTTGTCAGTGGAACTCCACAAACAAAGAAGCTGCAGGATCTGCTGCCATCAATTATCAACCAACTTGGTCCTGACAACTTGGATAATCTGCGGAGGCTTGCAGAGCAATTCCAAAAGCAGGCTCCTGGTGCTTCTGGTGAAGAGGCTGGTGCCAGTGCTGGAGCTGCTCAGGGTGACGATGATGATGTTCCTGAGCTTGTCCCTGGTGAGACATTTGAGGAGGCAGCAGAAGAGAAAAAGGAATCATGATTTTGTTGCATGTCTTCTCCTGTGAAGGATTATAAGTTGCGAATTGCCATTAGAGACCTCCGCTCTTAAAAACCTCTGACCTACCTTATTTGTGTCAGTGCTCGGTTTTGTCATGTACGAGGATTTGGTCCGGTATGTTGGTGGAATTTTACATCAATTAAATGCCTCTTTTGGTTGCCCCAGCCTTAATTTTTCCATAACTATAACCATTATAAGAACAGTAGAGCTCTTGCGTTATAATGTTGCTTAAAAATTCGCGAGGGTCCATGACCATTCGCGAGAGGGTCCATGACCATTATTAGAACAGCAGCTCTTGCATTCTAATGTCACTGAAATCTTGCTACCTGCTACCTGGTAAAGAAGTTGCTTCAGCATTCTAATGTC - >LOC_Os10g34180.2
AGCCTTACCCCTAGAGCCCTATCCTTTTCCTCTCCCCGAGTCTTCTTCGTCCACTCCCCCTATCCTCTCCTCCCACCGCGCCGCCGCCTCCGCCGCCGCCGCCGCGGCTCCCCCTCCTCCCCACAGCCAAGATGAATGTTGACAAGCTCAAGAAGATGGCAGGTGCTGTGCGCACCGGGGGGAAGGGTAGCGTGCGCAGAAAGAAGAAGGCAGTCCATAAGACTACAACCACAGATGACAAAAGACTTCAGAGCACTCTGAAAAGAGTAGGAGTAAACACCATCCCTGGCATTGAAGAGGTTAACATTTTCAAGGATGATGTGGTTATTCAGTTTCTGAATCCTAAAGTGCAAGCTTCAATTGGTGCTAATACATGGGTTGTCAGTGGAACTCCACAAACAAAGAAGCTGCAGGATCTGCTGCCATCAATTATCAACCAACTTGGTCCTGACAACTTGGATAATCTGCGGAGGCTTGCAGAGCAATTCCAAAAGCAGGCTCCTGGTGCTTCTGGTGAAGAGGCTGGTGCCAGTGCTGGAGCTGCTCAGGGTGACGATGATGATGTTCCTGAGCTTGTCCCTGGTGAGACATTTGAGGAGGCAGCAGAAGAGAAAAAGGAATCATGATTTTGTTGCATGTCTTCTCCTGTGAAGGATTATAAGTTGCGAATTGCCATTAGAGACCTCCGCTCTTAAAAACCTCTGACCTACCTTATTTGTGTCAGTGCTCGGTTTTGTCATGTACGAGGATTTGGTCCGGTATGTTGGTGGAATTTTACATCAATTAAATGCCTCTTTTGGTTGCCCCAGCCTTAATTTTTCCATAACTATAACCATTATAAGAACAGTAGAGCTCTTGCGTTATAATGTTGCTTAAAAATTCGCGAGGGTCCATGACCATTCGCGAGAGGGTCCATGACCATTATTAGAACAGCAGCTCTTGCATTCTAATGTCACTGAAATCTTGCTACCTGCTACCTGGTAAAGAAGTTGCTTCAGCATTCTAATGTC
CDS Sequence
- >LOC_Os10g34180.1
ATGAATGTTGACAAGCTCAAGAAGATGGCAGGTGCTGTGCGCACCGGGGGGAAGGGTAGCGTGCGCAGAAAGAAGAAGGCAGTCCATAAGACTACAACCACAGATGACAAAAGACTTCAGAGCACTCTGAAAAGAGTAGGAGTAAACACCATCCCTGGCATTGAAGAGGTTAACATTTTCAAGGATGATGTGGTTATTCAGTTTCTGAATCCTAAAGTGCAAGCTTCAATTGGTGCTAATACATGGGTTGTCAGTGGAACTCCACAAACAAAGAAGCTGCAGGATCTGCTGCCATCAATTATCAACCAACTTGGTCCTGACAACTTGGATAATCTGCGGAGGCTTGCAGAGCAATTCCAAAAGCAGGCTCCTGGTGCTTCTGGTGAAGAGGCTGGTGCCAGTGCTGGAGCTGCTCAGGGTGACGATGATGATGTTCCTGAGCTTGTCCCTGGTGAGACATTTGAGGAGGCAGCAGAAGAGAAAAAGGAATCATGA - >LOC_Os10g34180.2
ATGAATGTTGACAAGCTCAAGAAGATGGCAGGTGCTGTGCGCACCGGGGGGAAGGGTAGCGTGCGCAGAAAGAAGAAGGCAGTCCATAAGACTACAACCACAGATGACAAAAGACTTCAGAGCACTCTGAAAAGAGTAGGAGTAAACACCATCCCTGGCATTGAAGAGGTTAACATTTTCAAGGATGATGTGGTTATTCAGTTTCTGAATCCTAAAGTGCAAGCTTCAATTGGTGCTAATACATGGGTTGTCAGTGGAACTCCACAAACAAAGAAGCTGCAGGATCTGCTGCCATCAATTATCAACCAACTTGGTCCTGACAACTTGGATAATCTGCGGAGGCTTGCAGAGCAATTCCAAAAGCAGGCTCCTGGTGCTTCTGGTGAAGAGGCTGGTGCCAGTGCTGGAGCTGCTCAGGGTGACGATGATGATGTTCCTGAGCTTGTCCCTGGTGAGACATTTGAGGAGGCAGCAGAAGAGAAAAAGGAATCATGA
Protein Sequence
- >LOC_Os10g34180.1
MNVDKLKKMAGAVRTGGKGSVRRKKKAVHKTTTTDDKRLQSTLKRVGVNTIPGIEEVNIFKDDVVIQFLNPKVQASIGANTWVVSGTPQTKKLQDLLPSIINQLGPDNLDNLRRLAEQFQKQAPGASGEEAGASAGAAQGDDDDVPELVPGETFEEAAEEKKES* - >LOC_Os10g34180.2
MNVDKLKKMAGAVRTGGKGSVRRKKKAVHKTTTTDDKRLQSTLKRVGVNTIPGIEEVNIFKDDVVIQFLNPKVQASIGANTWVVSGTPQTKKLQDLLPSIINQLGPDNLDNLRRLAEQFQKQAPGASGEEAGASAGAAQGDDDDVPELVPGETFEEAAEEKKES*