Gene Details:
- MSU gene ID: LOC_Os10g33800
- RAPdb gene ID: Os10g0478200
- Gene Symbol: FLO16
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- In this study, we isolated a novel floury endosperm rice (Oryza sativa) mutant FLO16 with defective starch grain (SG) formation.
- Overexpression of FLO16 significantly improved grain weight, suggesting a possible application of FLO16 in rice breeding.
- Our results indicated that FLO16 plays a critical role in redox homeostasis that is important for compound SG formation and subsequent starch biosynthesis in rice endosperm.
- Map-based cloning and complementation tests demonstrated that FLO16 encodes a NAD-dependent cytosolic malate dehydrogenase (CMDH).
Function-related keywords:
- grain , starch , map-based-cloning , homeostasis , endosperm , breeding , grain-weight , starch-biosynthesis
Literature:
- FLOURY ENDOSPERM16 encoding a NAD-dependent cytosolic malate dehydrogenase plays an important role in starch synthesis and seed development in rice . DOI: 10.1111/pbi.13108 ; PMID: 30860317
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os10g33800.2
CGTTTCCCCCTCTCTCGCAAAACTCACCCAAAAGAGCAGCGTCGCCTCTCCTCCTCCTCCCTAACCCCTACGCTTCCAGAACCTTCTCGAAGCTCCCGCTCCCCCCCCCCTTCCGCTCCAATGGCGAAGGAACCGATGCGCGTGCTCGTCACCGGCGCCGCAGGACAAATTGGATATGCTCTTGTCCCCATGATTGCTAGGGGTGTGATGTTGGGTGCTGACCAGCCTGTTATTCTACACATGCTTGACATTCCACCAGCTACTGAATCTCTTAATGGCCTTAAGATGGAGCTGGTTGATGCTGCATTTCCTCTTTTGAAGGGAATTGTCGCAACAACTGATGTTGTGGAGGCCTGCACTGGTGTGAATGTTGCGGTTATGGTTGGTGGGTTCCCCAGGAAGGAGGGAATGGAAAGGAAGGATGTTATGTCAAAAAATGTCTCCATCTACAAATCCCAAGCTTCTGCTCTTGAGGCTCATGCAGCCCCTAACTGCAAGGTTCTGGTAGTTGCCAATCCAGCAAACACCAACGCTCTCATCTTAAAAGAATTCGCTCCATCCATCCCTGAGAAGAACATTACTTGCCTCACCCGTCTTGACCACAACAGGGCACTTGGCCAGATCTCTGAAAAACTTAATGTCCAAGTTACTGATGTGAAGAATGCGATCATCTGGGGCAACCACTCATCCACCCAGTACCCTGATGTTAACCACGCCACTGTGAAGACTCCCAGTGGAGAGAAGCCTGTCAGGGAACTCGTTGCTGATGATGAGTGGTTAAATACGGAATTCATCTCTACCGTCCAGCAGCGTGGTGCCGCCATCATCAAGGCGAGGAAGCAATCCAGTGCCCTATCTGCTGCCAGCTCTGCATGCGATCACATTCGTGACTGGGTTCTTGGCACTCCTGAGGGAACATTTGTCTCCATGGGTGTGTACTCTGATGGTTCGTATGGTGTGCCTGCTGGTCTGATCTACTCGTTCCCAGTAACATGCAGTGGTGGCGAATGGACGATTGTTCAGGGTATGCTTGCTTACCTTCTTTCTACATGTTCATGGGCTGTCTGTTAAAATGTGCCAATGTATAGAGTATTGCGCTGATTATGTCGCAACAGTTTACAAATGCCTGATGATCTATCGTTTCTTACAGGTCTCCCGATCGACGAGTTCTCAAGGAAGAAGATGGACGCGACTGCCCAGGAGCTGTCGGAGGAGAAGACGCTCGCTTACTCATGCCTCAACTAAAACTAAGCAATACCCAGAGGGACAGATAGTGAGCGATTGCCCGCTCCCGTGTTTTTGAATAAAAGAGACTTTTAAGTTCCATCACATAGAAACTGTTTATCTCAGACCGCTGCACATCGCGAGATGTGGAGCGCAGATGCCGTTGCTGGTTTTACTCCAGTGTGTATTGAGGCTTTGTACTAGCTCCCTTTTTTTTGCCTGGTGATTCGCAGGACATTTGCTGAAAACATTGAACCCATTTGACATCTGATGGAATCATGGACCAGTAGCAAGTACATTTTGCGAAAGCATAATCTGCATCGGGCTTGGGCTGGTGGTTGAACTTTCTGCCACATGGCCCATGGAAACGTGAGTCGCGCTGTTACTG - >LOC_Os10g33800.1
CGTTTCCCCCTCTCTCGCAAAACTCACCCAAAAGAGCAGCGTCGCCTCTCCTCCTCCTCCCTAACCCCTACGCTTCCAGAACCTTCTCGAAGCTCCCGCTCCCCCCCCCCTTCCGCTCCAATGGCGAAGGAACCGATGCGCGTGCTCGTCACCGGCGCCGCAGGACAAATTGGATATGCTCTTGTCCCCATGATTGCTAGGGGTGTGATGTTGGGTGCTGACCAGCCTGTTATTCTACACATGCTTGACATTCCACCAGCTACTGAATCTCTTAATGGCCTTAAGATGGAGCTGGTTGATGCTGCATTTCCTCTTTTGAAGGGAATTGTCGCAACAACTGATGTTGTGGAGGCCTGCACTGGTGTGAATGTTGCGGTTATGGTTGGTGGGTTCCCCAGGAAGGAGGGAATGGAAAGGAAGGATGTTATGTCAAAAAATGTCTCCATCTACAAATCCCAAGCTTCTGCTCTTGAGGCTCATGCAGCCCCTAACTGCAAGGTTCTGGTAGTTGCCAATCCAGCAAACACCAACGCTCTCATCTTAAAAGAATTCGCTCCATCCATCCCTGAGAAGAACATTACTTGCCTCACCCGTCTTGACCACAACAGGGCACTTGGCCAGATCTCTGAAAAACTTAATGTCCAAGTTACTGATGTGAAGAATGCGATCATCTGGGGCAACCACTCATCCACCCAGTACCCTGATGTTAACCACGCCACTGTGAAGACTCCCAGTGGAGAGAAGCCTGTCAGGGAACTCGTTGCTGATGATGAGTGGTTAAATACGGAATTCATCTCTACCGTCCAGCAGCGTGGTGCCGCCATCATCAAGGCGAGGAAGCAATCCAGTGCCCTATCTGCTGCCAGCTCTGCATGCGATCACATTCGTGACTGGGTTCTTGGCACTCCTGAGGGAACATTTGTCTCCATGGGTGTGTACTCTGATGGTTCGTATGGTGTGCCTGCTGGTCTGATCTACTCGTTCCCAGTAACATGCAGTGGTGGCGAATGGACGATTGTTCAGGGTATGCTTGCTTACCTTCTTTCTACATGTTCATGGGCTGTCTGTTAAAATGTGCCAATGTATAGAGTATTGCGCTGATTATGTCGCAACAGTTTACAAATGCCTGATGATCTATCGTTTCTTACAGGTCTCCCGATCGACGAGTTCTCAAGGAAGAAGATGGACGCGACTGCCCAGGAGCTGTCGGAGGAGAAGACGCTCGCTTACTCATGCCTCAACTAAAACTAAGCAATACCCAGAGGGACAGATAGTGAGCGATTGCCCGCTCCCGTGTTTTTGAATAAAAGAGACTTTTAAGTTCCATCACATAGAAACTGTTTATCTCAGACCGCTGCACATCGCGAGATGTGGAGCGCAGATGCCGTTGCTGGTTTTACTCCAGTGTGTATTGAGGCTTTGTACTAGCTCCCTTTTTTTTGCCTGGTGATTCGCAGGACATTTGCTGAAAACATTGAACCCATTTGACATCTGATGGAATCATGGACCAGTAGCAAGTACATTTTGCGAAAGCATAATCTGCATCGGGCTTGGGCTGGTGGTTGAACTTTCTGCCACATGGCCCATGGAAACGTGAGTCGCGCTGTTACTG - >LOC_Os10g33800.3
CGTTTCCCCCTCTCTCGCAAAACTCACCCAAAAGAGCAGCGTCGCCTCTCCTCCTCCTCCCTAACCCCTACGCTTCCAGAACCTTCTCGAAGCTCCCGCTCCCCCCCCCCTTCCGCTCCAATGGCGAAGGAACCGATGCGCGTGCTCGTCACCGGCGCCGCAGGACAAATTGGATATGCTCTTGTCCCCATGATTGCTAGGGGTGTGATGTTGGGTGCTGACCAGCCTGTTATTCTACACATGCTTGACATTCCACCAGCTACTGAATCTCTTAATGGCCTTAAGATGGAGCTGGTTGATGCTGCATTTCCTCTTTTGAAGGGAATTGTCGCAACAACTGATGTTGTGGAGGCCTGCACTGGTGTGAATGTTGCGGTTATGGTTGGTGGGTTCCCCAGGAAGGAGGGAATGGAAAGGAAGGATGTTATGTCAAAAAATGTCTCCATCTACAAATCCCAAGCTTCTGCTCTTGAGGCTCATGCAGCCCCTAACTGCAAGGTTCTGGTAGTTGCCAATCCAGCAAACACCAACGCTCTCATCTTAAAAGAATTCGCTCCATCCATCCCTGAGAAGAACATTACTTGCCTCACCCGTCTTGACCACAACAGGGCACTTGGCCAGATCTCTGAAAAACTTAATGTCCAAGTTACTGATGTGAAGAATGCGATCATCTGGGGCAACCACTCATCCACCCAGTACCCTGATGTTAACCACGCCACTGTGAAGACTCCCAGTGGAGAGAAGCCTGTCAGGGAACTCGTTGCTGATGATGAGTGGTTAAATACGGAATTCATCTCTACCGTCCAGCAGCGTGGTGCCGCCATCATCAAGGCGAGGAAGCAATCCAGTGCCCTATCTGCTGCCAGCTCTGCATGCGATCACATTCGTGACTGGGTTCTTGGCACTCCTGAGGGAACATTTGTCTCCATGGGTGTGTACTCTGATGGTTCGTATGGTGTGCCTGCTGGTCTGATCTACTCGTTCCCAGTAACATGCAGTGGTGGCGAATGGACGATTGTTCAGGGTATGCTTGCTTACCTTCTTTCTACATGTTCATGGGCTGTCTGTTAAAATGTGCCAATGTATAGAGTATTGCGCTGATTATGTCGCAACAGTTTACAAATGCCTGATGATCTATCGTTTCTTACAGGTCTCCCGATCGACGAGTTCTCAAGGAAGAAGATGGACGCGACTGCCCAGGAGCTGTCGGAGGAGAAGACGCTCGCTTACTCATGCCTCAACTAAAACTAAGCAATACCCAGAGGGACAGATAGTGAGCGATTGCCCGCTCCCGTGTTTTTGAATAAAAGAGACTTTTAAGTTCCATCACATAGAAACTGTTTATCTCAGACCGCTGCACATCGCGAGATGTGGAGCGCAGATGCCGTTGCTGGTTTTACTCCAGTGTGTATTGAGGCTTTGTACTAGCTCCCTTTTTTTTGCCTGGTGATTCGCAGGACATTTGCTGAAAACATTGAACCCATTTGACATCTGATGGAATCATGGACCAGTAGCAAGTACATTTTGCGAAAGCATAATCTGCATCGGGCTTGGGCTGGTGGTTGAACTTTCTGCCACATGGCCCATGGAAACGTGAGTCGCGCTGTTACTG
CDS Sequence
- >LOC_Os10g33800.2
ATGGCGAAGGAACCGATGCGCGTGCTCGTCACCGGCGCCGCAGGACAAATTGGATATGCTCTTGTCCCCATGATTGCTAGGGGTGTGATGTTGGGTGCTGACCAGCCTGTTATTCTACACATGCTTGACATTCCACCAGCTACTGAATCTCTTAATGGCCTTAAGATGGAGCTGGTTGATGCTGCATTTCCTCTTTTGAAGGGAATTGTCGCAACAACTGATGTTGTGGAGGCCTGCACTGGTGTGAATGTTGCGGTTATGGTTGGTGGGTTCCCCAGGAAGGAGGGAATGGAAAGGAAGGATGTTATGTCAAAAAATGTCTCCATCTACAAATCCCAAGCTTCTGCTCTTGAGGCTCATGCAGCCCCTAACTGCAAGGTTCTGGTAGTTGCCAATCCAGCAAACACCAACGCTCTCATCTTAAAAGAATTCGCTCCATCCATCCCTGAGAAGAACATTACTTGCCTCACCCGTCTTGACCACAACAGGGCACTTGGCCAGATCTCTGAAAAACTTAATGTCCAAGTTACTGATGTGAAGAATGCGATCATCTGGGGCAACCACTCATCCACCCAGTACCCTGATGTTAACCACGCCACTGTGAAGACTCCCAGTGGAGAGAAGCCTGTCAGGGAACTCGTTGCTGATGATGAGTGGTTAAATACGGAATTCATCTCTACCGTCCAGCAGCGTGGTGCCGCCATCATCAAGGCGAGGAAGCAATCCAGTGCCCTATCTGCTGCCAGCTCTGCATGCGATCACATTCGTGACTGGGTTCTTGGCACTCCTGAGGGAACATTTGTCTCCATGGGTGTGTACTCTGATGGTTCGTATGGTGTGCCTGCTGGTCTGATCTACTCGTTCCCAGTAACATGCAGTGGTGGCGAATGGACGATTGTTCAGGGTATGCTTGCTTACCTTCTTTCTACATGTTCATGGGCTGTCTGTTAA - >LOC_Os10g33800.1
ATGGCGAAGGAACCGATGCGCGTGCTCGTCACCGGCGCCGCAGGACAAATTGGATATGCTCTTGTCCCCATGATTGCTAGGGGTGTGATGTTGGGTGCTGACCAGCCTGTTATTCTACACATGCTTGACATTCCACCAGCTACTGAATCTCTTAATGGCCTTAAGATGGAGCTGGTTGATGCTGCATTTCCTCTTTTGAAGGGAATTGTCGCAACAACTGATGTTGTGGAGGCCTGCACTGGTGTGAATGTTGCGGTTATGGTTGGTGGGTTCCCCAGGAAGGAGGGAATGGAAAGGAAGGATGTTATGTCAAAAAATGTCTCCATCTACAAATCCCAAGCTTCTGCTCTTGAGGCTCATGCAGCCCCTAACTGCAAGGTTCTGGTAGTTGCCAATCCAGCAAACACCAACGCTCTCATCTTAAAAGAATTCGCTCCATCCATCCCTGAGAAGAACATTACTTGCCTCACCCGTCTTGACCACAACAGGGCACTTGGCCAGATCTCTGAAAAACTTAATGTCCAAGTTACTGATGTGAAGAATGCGATCATCTGGGGCAACCACTCATCCACCCAGTACCCTGATGTTAACCACGCCACTGTGAAGACTCCCAGTGGAGAGAAGCCTGTCAGGGAACTCGTTGCTGATGATGAGTGGTTAAATACGGAATTCATCTCTACCGTCCAGCAGCGTGGTGCCGCCATCATCAAGGCGAGGAAGCAATCCAGTGCCCTATCTGCTGCCAGCTCTGCATGCGATCACATTCGTGACTGGGTTCTTGGCACTCCTGAGGGAACATTTGTCTCCATGGGTGTGTACTCTGATGGTTCGTATGGTGTGCCTGCTGGTCTGATCTACTCGTTCCCAGTAACATGCAGTGGTGGCGAATGGACGATTGTTCAGGGTATGCTTGCTTACCTTCTTTCTACATGTTCATGGGCTGTCTGTTAA - >LOC_Os10g33800.3
ATGGCGAAGGAACCGATGCGCGTGCTCGTCACCGGCGCCGCAGGACAAATTGGATATGCTCTTGTCCCCATGATTGCTAGGGGTGTGATGTTGGGTGCTGACCAGCCTGTTATTCTACACATGCTTGACATTCCACCAGCTACTGAATCTCTTAATGGCCTTAAGATGGAGCTGGTTGATGCTGCATTTCCTCTTTTGAAGGGAATTGTCGCAACAACTGATGTTGTGGAGGCCTGCACTGGTGTGAATGTTGCGGTTATGGTTGGTGGGTTCCCCAGGAAGGAGGGAATGGAAAGGAAGGATGTTATGTCAAAAAATGTCTCCATCTACAAATCCCAAGCTTCTGCTCTTGAGGCTCATGCAGCCCCTAACTGCAAGGTTCTGGTAGTTGCCAATCCAGCAAACACCAACGCTCTCATCTTAAAAGAATTCGCTCCATCCATCCCTGAGAAGAACATTACTTGCCTCACCCGTCTTGACCACAACAGGGCACTTGGCCAGATCTCTGAAAAACTTAATGTCCAAGTTACTGATGTGAAGAATGCGATCATCTGGGGCAACCACTCATCCACCCAGTACCCTGATGTTAACCACGCCACTGTGAAGACTCCCAGTGGAGAGAAGCCTGTCAGGGAACTCGTTGCTGATGATGAGTGGTTAAATACGGAATTCATCTCTACCGTCCAGCAGCGTGGTGCCGCCATCATCAAGGCGAGGAAGCAATCCAGTGCCCTATCTGCTGCCAGCTCTGCATGCGATCACATTCGTGACTGGGTTCTTGGCACTCCTGAGGGAACATTTGTCTCCATGGGTGTGTACTCTGATGGTTCGTATGGTGTGCCTGCTGGTCTGATCTACTCGTTCCCAGTAACATGCAGTGGTGGCGAATGGACGATTGTTCAGGGTATGCTTGCTTACCTTCTTTCTACATGTTCATGGGCTGTCTGTTAA
Protein Sequence
- >LOC_Os10g33800.2
MAKEPMRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPATESLNGLKMELVDAAFPLLKGIVATTDVVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAHAAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVTDVKNAIIWGNHSSTQYPDVNHATVKTPSGEKPVRELVADDEWLNTEFISTVQQRGAAIIKARKQSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPAGLIYSFPVTCSGGEWTIVQGMLAYLLSTCSWAVC* - >LOC_Os10g33800.1
MAKEPMRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPATESLNGLKMELVDAAFPLLKGIVATTDVVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAHAAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVTDVKNAIIWGNHSSTQYPDVNHATVKTPSGEKPVRELVADDEWLNTEFISTVQQRGAAIIKARKQSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPAGLIYSFPVTCSGGEWTIVQGMLAYLLSTCSWAVC* - >LOC_Os10g33800.3
MAKEPMRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPATESLNGLKMELVDAAFPLLKGIVATTDVVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAHAAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVTDVKNAIIWGNHSSTQYPDVNHATVKTPSGEKPVRELVADDEWLNTEFISTVQQRGAAIIKARKQSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPAGLIYSFPVTCSGGEWTIVQGMLAYLLSTCSWAVC*