Information report for FLO7
Gene Details
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Functional Descriptions
- Notably, FLO7 peripheral endosperm cells showed obvious defects in compound starch grain development.
- Together, our findings identify FLO7 as a unique plant regulator required for starch synthesis and amyloplast development within the peripheral endosperm and provide new insights into the spatial regulation of endosperm development in rice.
- Map-based cloning of FLO7 revealed that it encodes a protein of unknown function.
- Consistent with the phenotypic alternation in FLO7 endosperm, the FLO7 mutant had reduced amylose content and seriously disrupted amylopectin structure only in the peripheral endosperm.
- FLO7 harbors an N-terminal transit peptide capable of targeting functional FLO7 fused to green fluorescent protein to amyloplast stroma in developing endosperm cells, and a domain of unknown function 1338 (DUF1338) that is highly conserved in green plants.
- Furthermore, our combined β-glucuronidase activity and RNA in situ hybridization assays showed that the FLO7 gene was expressed ubiquitously but exhibited a specific expression in the endosperm periphery.
- Moreover, a set of in vivo experiments demonstrated that the missing 32 aa in the FLO7 mutant protein are essential for the stable accumulation of FLO7 in the endosperm.
Functional Keywords
Literature and News
Sequences
cDNA Sequence
- >LOC_Os10g32680.3
GTCCACTCCACTCTCTCCTCCCCTTCAGCTGATCAGCTCGCCACGTCACCGTCACCGCCTCGCCTCGCTTGCTTGTCGTGGTGCAGCATCCAACCTAGTAGTAGTGGTAGTAGGCTAGGCTATATCCGCCTCGGCCACCGCCGGGTTCGCCTCCAGTGCTCGCCGCCTCCGCCGCCGCCGGTGTCCATGGCCGTGGCCCTTGCCGGCGCGAGATCCCCAGGAGCGGGCGCGATCCTCAGCCTCCGCCGCCTCGCCCCCGCCGCCGCGGCGCCCGTACGTCTGGGCGGAAGCGGAACCCCAGGCACCCGGCGCCGCCGCGGCATCGCCATGGCGGCAGCTGCGTCCGCCCCGCCCGCGCCCGCCGATGCCCTCCCCAAGGGTGCTGATTCATTTTTCCGGACAGTTATCTCAAACATGGAGAAAGTATATCTGAGCAGAAATCCAACTGCCAAAACCATCCTGGAACTTGTTCGTTCTTATGATGGGGACCACATTTGCTATGATCATTTTGCTTTCAGAACATTTGGAGTTGATGGTTATGGTATAAAATCACTTGCTGAATTTTTTACTGACTTTGGTTATGTCCCTCGTGAAGAACTAAGGTTTCCAGCAAAAAAGTTAAGAGCACTATGGTTTTCTCCTCCCACCAATGATGGATACACTGGAACTGGTGTTTATGGGCCTTTGCCAAGGATATTCATTTCAGAACTACTTGTGGATGAGTTGAGTCCTCAGAGCCAGGACATCATTCAAAAATACATTAGAACTTCTGGTAAAGGAAATAAACATGCAACTCTTGCAAGTACATCAGGGGAGCTGACATGGGAGAAGCCCATTTATTCTGATTTCCAAGTTTTATCTAGGGAAAGTGAATATGCTGCATGGACTCTTGTTAATGGCTATGCACTAAATCATACCACAATTTCCACTCATCGCTTGATATCAGATATTAGAAGTATCAATAAGTTCAACAAATTTGTGGAGGACAATGGGTTCAAGTTAAATTCAGAAGGAGGGATTTTGAAAGTGAGTCCTGATGGTCTCCTCCAGCAAAGCTCAACTGTAGCTGATTCAGCGTTGTTTACTTTTGCTGATGGAATCACTGAATCCATTCCACGTTCCTATATTGAATTTGCTGAACGCCTGGTACTCCCACAGTTCAAAGATTTGCCAAATGATGAGGCGCGATGGTTTTGAGGTAGGGAATGCGGACAAAATATTTGAGAGCACATCAAATGATCAGCTTACACGGAGATCTGCATAAACGTTGAAGTCTCAACTATTCTGGAAGCGAGTCATGCCTCTTGGAAGCGTATTGAAGGAGTGTTCTCTGTGATAACTGTATCTACCTGGGTAAATTAAAACATTAGAATGAATAAACTGTTCGATGTATATCGCTCTCTTGAATAAAGAAATTACTAGTTATCTCTACAGT - >LOC_Os10g32680.2
GTCCACTCCACTCTCTCCTCCCCTTCAGCTGATCAGCTCGCCACGTCACCGTCACCGCCTCGCCTCGCTTGCTTGTCGTGGTGCAGCATCCAACCTAGTAGTAGTGGTAGTAGGCTAGGCTATATCCGCCTCGGCCACCGCCGGGTTCGCCTCCAGTGCTCGCCGCCTCCGCCGCCGCCGGTGTCCATGGCCGTGGCCCTTGCCGGCGCGAGATCCCCAGGAGCGGGCGCGATCCTCAGCCTCCGCCGCCTCGCCCCCGCCGCCGCGGCGCCCGTACGTCTGGGCGGAAGCGGAACCCCAGGCACCCGGCGCCGCCGCGGCATCGCCATGGCGGCAGCTGCGTCCGCCCCGCCCGCGCCCGCCGATGCCCTCCCCAAGGGTGCTGATTCATTTTTCCGGACAGTTATCTCAAACATGGAGAAAGTATATCTGAGCAGAAATCCAACTGCCAAAACCATCCTGGAACTTGTTCGTTCTTATGATGGGGACCACATTTGCTATGATCATTTTGCTTTCAGAACATTTGGAGTTGATGGTTATGGTATAAAATCACTTGCTGAATTTTTTACTGACTTTGGTTATGTCCCTCGTGAAGAACTAAGGTTTCCAGCAAAAAAGTTAAGAGCACTATGGTTTTCTCCTCCCACCAATGATGGATACACTGGAACTGGTGTTTATGGGCCTTTGCCAAGGATATTCATTTCAGAACTACTTGTGGATGAGTTGAGTCCTCAGAGCCAGGACATCATTCAAAAATACATTAGAACTTCTGGTAAAGGAAATAAACATGCAACTCTTGCAAGTACATCAGGGGAGCTGACATGGGAGAAGCCCATTTATTCTGATTTCCAAGTTTTATCTAGGGAAAGTGAATATGCTGCATGGACTCTTGTTAATGGCTATGCACTAAATCATACCACAATTTCCACTCATCGCTTGATATCAGATATTAGAAGTATCAATAAGTTCAACAAATTTGTGGAGGACAATGGGTTCAAGTTAAATTCAGAAGGAGGGATTTTGAAAGTGAGTCCTGATGGTCTCCTCCAGCAAAGCTCAACTGTAGCTGATTCAGCGTTGTTTACTTTTGCTGATGGAATCACTGAATCCATTCCACGTTCCTATATTGAATTTGCTGAACGCCTGGTACTCCCACAGTTCAAAGATTTGCCAAATGATGAGGCGCGATGGTTTTGAGGTAGGGAATGCGGACAAAATATTTGAGAGCACATCAAATGATCAGCTTACACGGAGATCTGCATAAACGTTGAAGTCTCAACTATTCTGGAAGCGAGTCATGCCTCTTGGAAGCGTATTGAAGGAGTGTTCTCTGTGATAACTGTATCTACCTGGGTAAATTAAAACATTAGAATGAATAAACTGTTCGATGTATATCGCTCTCTTGAATAAAGAAATTACTAGTTATCTCTACAGT - >LOC_Os10g32680.1
GTCCACTCCACTCTCTCCTCCCCTTCAGCTGATCAGCTCGCCACGTCACCGTCACCGCCTCGCCTCGCTTGCTTGTCGTGGTGCAGCATCCAACCTAGTAGTAGTGGTAGTAGGCTAGGCTATATCCGCCTCGGCCACCGCCGGGTTCGCCTCCAGTGCTCGCCGCCTCCGCCGCCGCCGGTGTCCATGGCCGTGGCCCTTGCCGGCGCGAGATCCCCAGGAGCGGGCGCGATCCTCAGCCTCCGCCGCCTCGCCCCCGCCGCCGCGGCGCCCGTACGTCTGGGCGGAAGCGGAACCCCAGGCACCCGGCGCCGCCGCGGCATCGCCATGGCGGCAGCTGCGTCCGCCCCGCCCGCGCCCGCCGATGCCCTCCCCAAGGGTGCTGATTCATTTTTCCGGACAGTTATCTCAAACATGGAGAAAGTATATCTGAGCAGAAATCCAACTGCCAAAACCATCCTGGAACTTGTTCGTTCTTATGATGGGGACCACATTTGCTATGATCATTTTGCTTTCAGAACATTTGGAGTTGATGGTTATGGTATAAAATCACTTGCTGAATTTTTTACTGACTTTGGTTATGTCCCTCGTGAAGAACTAAGGTTTCCAGCAAAAAAGTTAAGAGCACTATGGTTTTCTCCTCCCACCAATGATGGATACACTGGAACTGGTGTTTATGGGCCTTTGCCAAGGATATTCATTTCAGAACTACTTGTGGATGAGTTGAGTCCTCAGAGCCAGGACATCATTCAAAAATACATTAGAACTTCTGGTAAAGGAAATAAACATGCAACTCTTGCAAGTACATCAGGGGAGCTGACATGGGAGAAGCCCATTTATTCTGATTTCCAAGTTTTATCTAGGGAAAGTGAATATGCTGCATGGACTCTTGTTAATGGCTATGCACTAAATCATACCACAATTTCCACTCATCGCTTGATATCAGATATTAGAAGTATCAATAAGTTCAACAAATTTGTGGAGGACAATGGGTTCAAGTTAAATTCAGAAGGAGGGATTTTGAAAGTGAGTCCTGATGGTCTCCTCCAGCAAAGCTCAACTGTAGCTGATTCAGCGTTGTTTACTTTTGCTGATGGAATCACTGAATCCATTCCACGTTCCTATATTGAATTTGCTGAACGCCTGGTACTCCCACAGTTCAAAGATTTGCCAAATGATGAGGCGCGATGGTTTTGAGGTAGGGAATGCGGACAAAATATTTGAGAGCACATCAAATGATCAGCTTACACGGAGATCTGCATAAACGTTGAAGTCTCAACTATTCTGGAAGCGAGTCATGCCTCTTGGAAGCGTATTGAAGGAGTGTTCTCTGTGATAACTGTATCTACCTGGGTAAATTAAAACATTAGAATGAATAAACTGTTCGATGTATATCGCTCTCTTGAATAAAGAAATTACTAGTTATCTCTACAGT
CDS Sequence
- >LOC_Os10g32680.3
ATGGCCGTGGCCCTTGCCGGCGCGAGATCCCCAGGAGCGGGCGCGATCCTCAGCCTCCGCCGCCTCGCCCCCGCCGCCGCGGCGCCCGTACGTCTGGGCGGAAGCGGAACCCCAGGCACCCGGCGCCGCCGCGGCATCGCCATGGCGGCAGCTGCGTCCGCCCCGCCCGCGCCCGCCGATGCCCTCCCCAAGGGTGCTGATTCATTTTTCCGGACAGTTATCTCAAACATGGAGAAAGTATATCTGAGCAGAAATCCAACTGCCAAAACCATCCTGGAACTTGTTCGTTCTTATGATGGGGACCACATTTGCTATGATCATTTTGCTTTCAGAACATTTGGAGTTGATGGTTATGGTATAAAATCACTTGCTGAATTTTTTACTGACTTTGGTTATGTCCCTCGTGAAGAACTAAGGTTTCCAGCAAAAAAGTTAAGAGCACTATGGTTTTCTCCTCCCACCAATGATGGATACACTGGAACTGGTGTTTATGGGCCTTTGCCAAGGATATTCATTTCAGAACTACTTGTGGATGAGTTGAGTCCTCAGAGCCAGGACATCATTCAAAAATACATTAGAACTTCTGGTAAAGGAAATAAACATGCAACTCTTGCAAGTACATCAGGGGAGCTGACATGGGAGAAGCCCATTTATTCTGATTTCCAAGTTTTATCTAGGGAAAGTGAATATGCTGCATGGACTCTTGTTAATGGCTATGCACTAAATCATACCACAATTTCCACTCATCGCTTGATATCAGATATTAGAAGTATCAATAAGTTCAACAAATTTGTGGAGGACAATGGGTTCAAGTTAAATTCAGAAGGAGGGATTTTGAAAGTGAGTCCTGATGGTCTCCTCCAGCAAAGCTCAACTGTAGCTGATTCAGCGTTGTTTACTTTTGCTGATGGAATCACTGAATCCATTCCACGTTCCTATATTGAATTTGCTGAACGCCTGGTACTCCCACAGTTCAAAGATTTGCCAAATGATGAGGCGCGATGGTTTTGA - >LOC_Os10g32680.2
ATGGCCGTGGCCCTTGCCGGCGCGAGATCCCCAGGAGCGGGCGCGATCCTCAGCCTCCGCCGCCTCGCCCCCGCCGCCGCGGCGCCCGTACGTCTGGGCGGAAGCGGAACCCCAGGCACCCGGCGCCGCCGCGGCATCGCCATGGCGGCAGCTGCGTCCGCCCCGCCCGCGCCCGCCGATGCCCTCCCCAAGGGTGCTGATTCATTTTTCCGGACAGTTATCTCAAACATGGAGAAAGTATATCTGAGCAGAAATCCAACTGCCAAAACCATCCTGGAACTTGTTCGTTCTTATGATGGGGACCACATTTGCTATGATCATTTTGCTTTCAGAACATTTGGAGTTGATGGTTATGGTATAAAATCACTTGCTGAATTTTTTACTGACTTTGGTTATGTCCCTCGTGAAGAACTAAGGTTTCCAGCAAAAAAGTTAAGAGCACTATGGTTTTCTCCTCCCACCAATGATGGATACACTGGAACTGGTGTTTATGGGCCTTTGCCAAGGATATTCATTTCAGAACTACTTGTGGATGAGTTGAGTCCTCAGAGCCAGGACATCATTCAAAAATACATTAGAACTTCTGGTAAAGGAAATAAACATGCAACTCTTGCAAGTACATCAGGGGAGCTGACATGGGAGAAGCCCATTTATTCTGATTTCCAAGTTTTATCTAGGGAAAGTGAATATGCTGCATGGACTCTTGTTAATGGCTATGCACTAAATCATACCACAATTTCCACTCATCGCTTGATATCAGATATTAGAAGTATCAATAAGTTCAACAAATTTGTGGAGGACAATGGGTTCAAGTTAAATTCAGAAGGAGGGATTTTGAAAGTGAGTCCTGATGGTCTCCTCCAGCAAAGCTCAACTGTAGCTGATTCAGCGTTGTTTACTTTTGCTGATGGAATCACTGAATCCATTCCACGTTCCTATATTGAATTTGCTGAACGCCTGGTACTCCCACAGTTCAAAGATTTGCCAAATGATGAGGCGCGATGGTTTTGA - >LOC_Os10g32680.1
ATGGCCGTGGCCCTTGCCGGCGCGAGATCCCCAGGAGCGGGCGCGATCCTCAGCCTCCGCCGCCTCGCCCCCGCCGCCGCGGCGCCCGTACGTCTGGGCGGAAGCGGAACCCCAGGCACCCGGCGCCGCCGCGGCATCGCCATGGCGGCAGCTGCGTCCGCCCCGCCCGCGCCCGCCGATGCCCTCCCCAAGGGTGCTGATTCATTTTTCCGGACAGTTATCTCAAACATGGAGAAAGTATATCTGAGCAGAAATCCAACTGCCAAAACCATCCTGGAACTTGTTCGTTCTTATGATGGGGACCACATTTGCTATGATCATTTTGCTTTCAGAACATTTGGAGTTGATGGTTATGGTATAAAATCACTTGCTGAATTTTTTACTGACTTTGGTTATGTCCCTCGTGAAGAACTAAGGTTTCCAGCAAAAAAGTTAAGAGCACTATGGTTTTCTCCTCCCACCAATGATGGATACACTGGAACTGGTGTTTATGGGCCTTTGCCAAGGATATTCATTTCAGAACTACTTGTGGATGAGTTGAGTCCTCAGAGCCAGGACATCATTCAAAAATACATTAGAACTTCTGGTAAAGGAAATAAACATGCAACTCTTGCAAGTACATCAGGGGAGCTGACATGGGAGAAGCCCATTTATTCTGATTTCCAAGTTTTATCTAGGGAAAGTGAATATGCTGCATGGACTCTTGTTAATGGCTATGCACTAAATCATACCACAATTTCCACTCATCGCTTGATATCAGATATTAGAAGTATCAATAAGTTCAACAAATTTGTGGAGGACAATGGGTTCAAGTTAAATTCAGAAGGAGGGATTTTGAAAGTGAGTCCTGATGGTCTCCTCCAGCAAAGCTCAACTGTAGCTGATTCAGCGTTGTTTACTTTTGCTGATGGAATCACTGAATCCATTCCACGTTCCTATATTGAATTTGCTGAACGCCTGGTACTCCCACAGTTCAAAGATTTGCCAAATGATGAGGCGCGATGGTTTTGA
Protein Sequence
- >LOC_Os10g32680.3
MAVALAGARSPGAGAILSLRRLAPAAAAPVRLGGSGTPGTRRRRGIAMAAAASAPPAPADALPKGADSFFRTVISNMEKVYLSRNPTAKTILELVRSYDGDHICYDHFAFRTFGVDGYGIKSLAEFFTDFGYVPREELRFPAKKLRALWFSPPTNDGYTGTGVYGPLPRIFISELLVDELSPQSQDIIQKYIRTSGKGNKHATLASTSGELTWEKPIYSDFQVLSRESEYAAWTLVNGYALNHTTISTHRLISDIRSINKFNKFVEDNGFKLNSEGGILKVSPDGLLQQSSTVADSALFTFADGITESIPRSYIEFAERLVLPQFKDLPNDEARWF* - >LOC_Os10g32680.2
MAVALAGARSPGAGAILSLRRLAPAAAAPVRLGGSGTPGTRRRRGIAMAAAASAPPAPADALPKGADSFFRTVISNMEKVYLSRNPTAKTILELVRSYDGDHICYDHFAFRTFGVDGYGIKSLAEFFTDFGYVPREELRFPAKKLRALWFSPPTNDGYTGTGVYGPLPRIFISELLVDELSPQSQDIIQKYIRTSGKGNKHATLASTSGELTWEKPIYSDFQVLSRESEYAAWTLVNGYALNHTTISTHRLISDIRSINKFNKFVEDNGFKLNSEGGILKVSPDGLLQQSSTVADSALFTFADGITESIPRSYIEFAERLVLPQFKDLPNDEARWF* - >LOC_Os10g32680.1
MAVALAGARSPGAGAILSLRRLAPAAAAPVRLGGSGTPGTRRRRGIAMAAAASAPPAPADALPKGADSFFRTVISNMEKVYLSRNPTAKTILELVRSYDGDHICYDHFAFRTFGVDGYGIKSLAEFFTDFGYVPREELRFPAKKLRALWFSPPTNDGYTGTGVYGPLPRIFISELLVDELSPQSQDIIQKYIRTSGKGNKHATLASTSGELTWEKPIYSDFQVLSRESEYAAWTLVNGYALNHTTISTHRLISDIRSINKFNKFVEDNGFKLNSEGGILKVSPDGLLQQSSTVADSALFTFADGITESIPRSYIEFAERLVLPQFKDLPNDEARWF*