Gene Details:
- MSU gene ID: LOC_Os10g06000
- RAPdb gene ID: Os10g0150800
- Gene Symbol: OsPRP1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- The OsPRP1 mutant plants displayed more sensitivity to cold stress and showed low survival rate with decreased root biomass than wild-type (WT) and homozygous mutant line with large fragment deletion was more sensitive to low temperature.
- These results suggested that OsPRP1 enhances cold tolerance by modulating antioxidants and maintaining cross talk through signaling pathways.
- Therefore, OsPRP1 gene could be exploited for improving cold tolerance in rice and CRISPR/Cas9 technology is helpful to study the function of a gene by analyzing the phenotypes of knockout mutants generated.
- Mutant plants were generated by CRISPR/Cas9 technology to investigate the role of OsPRP1 in cold stress and 26 mutant plants were obtained in T0 generation with the mutation rate of 85% including 15% bi-allelic, 53.
Function-related keywords:
- root , temperature , tolerance , cold-tolerance , cold-stress , stress , biomass
Literature:
- Knockout of OsPRP1, a gene encoding proline-rich protein, confers enhanced cold sensitivity in rice (Oryza sativa L.) at the seedling stage . DOI: 10.1007/s13205-019-1787-4 ; PMID: 31192079
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os10g06000.1
TCATCCATCTCTACACATCAGCAATAGCTACACAGAGCTAAGCTAGGGTTAGCTCAGCAATGGCAGGTGCTCCTCGAGGACTAGTTCTCCTCGGCGTTTGTGCCGTCTTGATGGCGGTCGCCGTCGGCGGAGAGGCGGCGTCGGTCGTCGTCGGCACGGCCAAGTGCGCCGACTGCACCAGGAAGAACATGAAAGCTGAGGATGCTTTCAAGAACCTCCAGGTGGCGATCAAGTGCAAGAACGGCAATGGTGAGTACGAGAGCAAGGCCACCGGAAAGCTCGACGGTACCGGCGTCTTCAGCATCCCCCTCGACGCCGACCTCCACAGCTCCGACTGCGTCGCACAGCTCCATGGCGCGACCAACGAGCCATGCCCCGGACAAGAGCCATCCAAGATCGTGCCAATGTCAGAGGGCACCTTTATCGCCGTCGCCGGCCAGACCCACTACCCATCAGCGTTGTGCGCATCGGCGACCATCTGCGGTCCCATCAAGAAGAAGATCATAGACCACTTCCACAAGAAGCCGGTGCCACCCAAGCCAGAGCCAAAACCGGAGCCACCCAAGCCCAAGCCTGAGCCGGAGCACCCATTCCTCGACCACATCCACAAGAAGGAGAAGCACTTCTTCGATCACTTCCACAAGAAGCCCGTGCCGCCCAAGCCAGAGCCCAAGCCGGAGCCCAAGCCAGAGCCCAAGCCGCAGCCTGCGCCTGAGTACCACAACCCGAGCCCTCCGGCGAAGAATTGATGAATATGATCTGCATTGGATGGACATTAGCATGTTTGATCATGCCGCAGATGATGATCCTGCTCAGCACAGGAGTGTAATTTGTACTTGATTATCGGGCATCAGCAGGGATGTTTGTACTATTTGCATATTTTTCTCTCCCAAATGAAATTATATATGGTAAAATAATTTATTTACATTTAGCCAGATTTGGTGGGACGACTGATTATTCACCATTTAGCATTCACCTTAGCTTGATTC
CDS Sequence
- >LOC_Os10g06000.1
ATGGCAGGTGCTCCTCGAGGACTAGTTCTCCTCGGCGTTTGTGCCGTCTTGATGGCGGTCGCCGTCGGCGGAGAGGCGGCGTCGGTCGTCGTCGGCACGGCCAAGTGCGCCGACTGCACCAGGAAGAACATGAAAGCTGAGGATGCTTTCAAGAACCTCCAGGTGGCGATCAAGTGCAAGAACGGCAATGGTGAGTACGAGAGCAAGGCCACCGGAAAGCTCGACGGTACCGGCGTCTTCAGCATCCCCCTCGACGCCGACCTCCACAGCTCCGACTGCGTCGCACAGCTCCATGGCGCGACCAACGAGCCATGCCCCGGACAAGAGCCATCCAAGATCGTGCCAATGTCAGAGGGCACCTTTATCGCCGTCGCCGGCCAGACCCACTACCCATCAGCGTTGTGCGCATCGGCGACCATCTGCGGTCCCATCAAGAAGAAGATCATAGACCACTTCCACAAGAAGCCGGTGCCACCCAAGCCAGAGCCAAAACCGGAGCCACCCAAGCCCAAGCCTGAGCCGGAGCACCCATTCCTCGACCACATCCACAAGAAGGAGAAGCACTTCTTCGATCACTTCCACAAGAAGCCCGTGCCGCCCAAGCCAGAGCCCAAGCCGGAGCCCAAGCCAGAGCCCAAGCCGCAGCCTGCGCCTGAGTACCACAACCCGAGCCCTCCGGCGAAGAATTGA
Protein Sequence
- >LOC_Os10g06000.1
MAGAPRGLVLLGVCAVLMAVAVGGEAASVVVGTAKCADCTRKNMKAEDAFKNLQVAIKCKNGNGEYESKATGKLDGTGVFSIPLDADLHSSDCVAQLHGATNEPCPGQEPSKIVPMSEGTFIAVAGQTHYPSALCASATICGPIKKKIIDHFHKKPVPPKPEPKPEPPKPKPEPEHPFLDHIHKKEKHFFDHFHKKPVPPKPEPKPEPKPEPKPQPAPEYHNPSPPAKN*