Gene Details:

Functional Descriptions:

  • The OsPRP1 mutant plants displayed more sensitivity to cold stress and showed low survival rate with decreased root biomass than wild-type (WT) and homozygous mutant line with large fragment deletion was more sensitive to low temperature.
  • These results suggested that OsPRP1 enhances cold tolerance by modulating antioxidants and maintaining cross talk through signaling pathways.
  • Therefore, OsPRP1 gene could be exploited for improving cold tolerance in rice and CRISPR/Cas9 technology is helpful to study the function of a gene by analyzing the phenotypes of knockout mutants generated.
  • Mutant plants were generated by CRISPR/Cas9 technology to investigate the role of OsPRP1 in cold stress and 26 mutant plants were obtained in T0 generation with the mutation rate of 85% including 15% bi-allelic, 53.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os10g06000.1
    TCATCCATCTCTACACATCAGCAATAGCTACACAGAGCTAAGCTAGGGTTAGCTCAGCAATGGCAGGTGCTCCTCGAGGACTAGTTCTCCTCGGCGTTTGTGCCGTCTTGATGGCGGTCGCCGTCGGCGGAGAGGCGGCGTCGGTCGTCGTCGGCACGGCCAAGTGCGCCGACTGCACCAGGAAGAACATGAAAGCTGAGGATGCTTTCAAGAACCTCCAGGTGGCGATCAAGTGCAAGAACGGCAATGGTGAGTACGAGAGCAAGGCCACCGGAAAGCTCGACGGTACCGGCGTCTTCAGCATCCCCCTCGACGCCGACCTCCACAGCTCCGACTGCGTCGCACAGCTCCATGGCGCGACCAACGAGCCATGCCCCGGACAAGAGCCATCCAAGATCGTGCCAATGTCAGAGGGCACCTTTATCGCCGTCGCCGGCCAGACCCACTACCCATCAGCGTTGTGCGCATCGGCGACCATCTGCGGTCCCATCAAGAAGAAGATCATAGACCACTTCCACAAGAAGCCGGTGCCACCCAAGCCAGAGCCAAAACCGGAGCCACCCAAGCCCAAGCCTGAGCCGGAGCACCCATTCCTCGACCACATCCACAAGAAGGAGAAGCACTTCTTCGATCACTTCCACAAGAAGCCCGTGCCGCCCAAGCCAGAGCCCAAGCCGGAGCCCAAGCCAGAGCCCAAGCCGCAGCCTGCGCCTGAGTACCACAACCCGAGCCCTCCGGCGAAGAATTGATGAATATGATCTGCATTGGATGGACATTAGCATGTTTGATCATGCCGCAGATGATGATCCTGCTCAGCACAGGAGTGTAATTTGTACTTGATTATCGGGCATCAGCAGGGATGTTTGTACTATTTGCATATTTTTCTCTCCCAAATGAAATTATATATGGTAAAATAATTTATTTACATTTAGCCAGATTTGGTGGGACGACTGATTATTCACCATTTAGCATTCACCTTAGCTTGATTC
CDS Sequence
  • >LOC_Os10g06000.1
    ATGGCAGGTGCTCCTCGAGGACTAGTTCTCCTCGGCGTTTGTGCCGTCTTGATGGCGGTCGCCGTCGGCGGAGAGGCGGCGTCGGTCGTCGTCGGCACGGCCAAGTGCGCCGACTGCACCAGGAAGAACATGAAAGCTGAGGATGCTTTCAAGAACCTCCAGGTGGCGATCAAGTGCAAGAACGGCAATGGTGAGTACGAGAGCAAGGCCACCGGAAAGCTCGACGGTACCGGCGTCTTCAGCATCCCCCTCGACGCCGACCTCCACAGCTCCGACTGCGTCGCACAGCTCCATGGCGCGACCAACGAGCCATGCCCCGGACAAGAGCCATCCAAGATCGTGCCAATGTCAGAGGGCACCTTTATCGCCGTCGCCGGCCAGACCCACTACCCATCAGCGTTGTGCGCATCGGCGACCATCTGCGGTCCCATCAAGAAGAAGATCATAGACCACTTCCACAAGAAGCCGGTGCCACCCAAGCCAGAGCCAAAACCGGAGCCACCCAAGCCCAAGCCTGAGCCGGAGCACCCATTCCTCGACCACATCCACAAGAAGGAGAAGCACTTCTTCGATCACTTCCACAAGAAGCCCGTGCCGCCCAAGCCAGAGCCCAAGCCGGAGCCCAAGCCAGAGCCCAAGCCGCAGCCTGCGCCTGAGTACCACAACCCGAGCCCTCCGGCGAAGAATTGA
Protein Sequence
  • >LOC_Os10g06000.1
    MAGAPRGLVLLGVCAVLMAVAVGGEAASVVVGTAKCADCTRKNMKAEDAFKNLQVAIKCKNGNGEYESKATGKLDGTGVFSIPLDADLHSSDCVAQLHGATNEPCPGQEPSKIVPMSEGTFIAVAGQTHYPSALCASATICGPIKKKIIDHFHKKPVPPKPEPKPEPPKPKPEPEHPFLDHIHKKEKHFFDHFHKKPVPPKPEPKPEPKPEPKPQPAPEYHNPSPPAKN*