Gene Details:
- MSU gene ID: LOC_Os09g39380
- RAPdb gene ID: Os09g0567300
- Gene Symbol: OsMDHAR
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- High OsMDHAR expression also improved the fermentative capacity of the yeast during glucose-based batch fermentation at a standard cultivation temperature (30°C).
- To examine whether heterologous expression of MDHAR from Oryza sativa (OsMDHAR) can prevent the deleterious effects of unfavorable growth conditions, we constructed a transgenic yeast strain harboring a recombinant plasmid carrying OsMDHAR (p426GPD::OsMDHAR).
- These results suggest that heterologous OsMDHAR expression increases tolerance to reactive oxygen species-induced oxidative stress by improving cellular redox homeostasis and improves survival during fermentation, which enhances fermentative capacity.
Function-related keywords:
- temperature , growth , oxidative-stress , tolerance , oxidative , stress , homeostasis , reactive-oxygen-species
Literature:
- Potential Application of the Oryza sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces cerevisiae . DOI: 10.1371/journal.pone.0158841 ; PMID: 27392090
- Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica . DOI: 10.1038/srep33903 ; PMID: 27652777
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os09g39380.1
ATGGCGTCGGAGAAGCACTTCAAGTACGTCATCCTCGGCGGCGGCGTCGCAGCGGGATATGCGGCACGGGAGTTCGCCAAGCAGGGTGTTAAGCCAGGGGAGCTCGCCATCATCTCCAAGGAGGCCGTACTTTTACTTACTGGAACCATACCTGTTCTTGTTGTGGCTCCTTATGAGCGCCCTGCTCTCAGCAAGGGATACCTCTTTCCTCAGAATGCTGCAAGACTCCCAGGATTTCATGTGTGTGTCGGCAGTGGAGGAGAGAGGCTTTTGCCCGAATGGTACTCAGAGAAAGGCATTGAGTTGATCCTGAGCACTGAAATTGTCAAAGCTGATCTTGCCTCCAAGACTTTGACTAGTGCAGTTGGAGCAACCTTTACATATGAGATTTTGATCATTGCAACTGGCTCCTCAGTCATCAAGCTATCAGATTTTGGTACTCAAGGAGCTGATTCCAACAACATTTTATATCTAAGGGAAGTAGATGATGCTGACAAGCTGGTTGCAGCTATCCAAGCAAAGAAGGGTGGAAAGGCAGTTATTGTAGGAGGAGGTTACATTGGTCTAGAACTTAGTGCTGCATTGAAGATCAATGATTTTGATGTCACGATGGTGTTTCCTGAACCTTGGTGCATGCCTCGTCTCTTCACTGCTGATATCGCGGCTTTCTATGAGAGTTACTACACTAACAAAGGAGTTAAGATCGTGAAGGGTACAGTAGCTGTTGGTTTTGATGCTGATGCTAATGGTGATGTCACAGCAGTTAACCTGAAGAATGGCAGTGTGCTTGAAGCTGATATTGTTGTTGTTGGTGTTGGGGGCAGACCGCTGACTACTCTCTTTAAAGGTCAAGTTGCTGAGGAGAAAGGTGGAATTAAGACCGATGCTTTCTTTGAAACAAGTGTTCCTGGAGTCTATGCTGTCGGTGATGTGGCCACCTTCCCCATGAAGATGTACAATGAGTTGAGGAGAGTGGAACATGTTGACCATGCTAGGAAGTCTGCAGAGCAGGCTGTAAAGGCAATCAAGGGAAAAGAGTCCGGCGAGTCCGTTGTGGAGTATGACTATCTGCCATACTTCTACTCCCGGTCATTCGACCTGGGATGGCAATTCTACGGCGACAACGTTGGCGACACCATCCTGTTCGGAGACAGTGACCCGACCTCTGCCAAGCCCAAGTTCGGGTCCTACTGGATCAAGGACGGCAAGGTGTTGGGTGCCTTCCTGGAGGGTGGGTCACCTGATGAGAACAAGGCCATTGCCAAGGTCGCGAAAACTCAGCCGCCCGTTGCCAACATAGAGGAGCTCAAGAAGGAAGGCCTCCAGTTCGCCAGCAAAATATGA
CDS Sequence
- >LOC_Os09g39380.1
ATGGCGTCGGAGAAGCACTTCAAGTACGTCATCCTCGGCGGCGGCGTCGCAGCGGGATATGCGGCACGGGAGTTCGCCAAGCAGGGTGTTAAGCCAGGGGAGCTCGCCATCATCTCCAAGGAGGCCGTACTTTTACTTACTGGAACCATACCTGTTCTTGTTGTGGCTCCTTATGAGCGCCCTGCTCTCAGCAAGGGATACCTCTTTCCTCAGAATGCTGCAAGACTCCCAGGATTTCATGTGTGTGTCGGCAGTGGAGGAGAGAGGCTTTTGCCCGAATGGTACTCAGAGAAAGGCATTGAGTTGATCCTGAGCACTGAAATTGTCAAAGCTGATCTTGCCTCCAAGACTTTGACTAGTGCAGTTGGAGCAACCTTTACATATGAGATTTTGATCATTGCAACTGGCTCCTCAGTCATCAAGCTATCAGATTTTGGTACTCAAGGAGCTGATTCCAACAACATTTTATATCTAAGGGAAGTAGATGATGCTGACAAGCTGGTTGCAGCTATCCAAGCAAAGAAGGGTGGAAAGGCAGTTATTGTAGGAGGAGGTTACATTGGTCTAGAACTTAGTGCTGCATTGAAGATCAATGATTTTGATGTCACGATGGTGTTTCCTGAACCTTGGTGCATGCCTCGTCTCTTCACTGCTGATATCGCGGCTTTCTATGAGAGTTACTACACTAACAAAGGAGTTAAGATCGTGAAGGGTACAGTAGCTGTTGGTTTTGATGCTGATGCTAATGGTGATGTCACAGCAGTTAACCTGAAGAATGGCAGTGTGCTTGAAGCTGATATTGTTGTTGTTGGTGTTGGGGGCAGACCGCTGACTACTCTCTTTAAAGGTCAAGTTGCTGAGGAGAAAGGTGGAATTAAGACCGATGCTTTCTTTGAAACAAGTGTTCCTGGAGTCTATGCTGTCGGTGATGTGGCCACCTTCCCCATGAAGATGTACAATGAGTTGAGGAGAGTGGAACATGTTGACCATGCTAGGAAGTCTGCAGAGCAGGCTGTAAAGGCAATCAAGGGAAAAGAGTCCGGCGAGTCCGTTGTGGAGTATGACTATCTGCCATACTTCTACTCCCGGTCATTCGACCTGGGATGGCAATTCTACGGCGACAACGTTGGCGACACCATCCTGTTCGGAGACAGTGACCCGACCTCTGCCAAGCCCAAGTTCGGGTCCTACTGGATCAAGGACGGCAAGGTGTTGGGTGCCTTCCTGGAGGGTGGGTCACCTGATGAGAACAAGGCCATTGCCAAGGTCGCGAAAACTCAGCCGCCCGTTGCCAACATAGAGGAGCTCAAGAAGGAAGGCCTCCAGTTCGCCAGCAAAATATGA
Protein Sequence
- >LOC_Os09g39380.1
MASEKHFKYVILGGGVAAGYAAREFAKQGVKPGELAIISKEAVLLLTGTIPVLVVAPYERPALSKGYLFPQNAARLPGFHVCVGSGGERLLPEWYSEKGIELILSTEIVKADLASKTLTSAVGATFTYEILIIATGSSVIKLSDFGTQGADSNNILYLREVDDADKLVAAIQAKKGGKAVIVGGGYIGLELSAALKINDFDVTMVFPEPWCMPRLFTADIAAFYESYYTNKGVKIVKGTVAVGFDADANGDVTAVNLKNGSVLEADIVVVGVGGRPLTTLFKGQVAEEKGGIKTDAFFETSVPGVYAVGDVATFPMKMYNELRRVEHVDHARKSAEQAVKAIKGKESGESVVEYDYLPYFYSRSFDLGWQFYGDNVGDTILFGDSDPTSAKPKFGSYWIKDGKVLGAFLEGGSPDENKAIAKVAKTQPPVANIEELKKEGLQFASKI*