Information report for OsMDHAR
Gene Details
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Functional Descriptions
- High OsMDHAR expression also improved the fermentative capacity of the yeast during glucose-based batch fermentation at a standard cultivation temperature (30°C).
- To examine whether heterologous expression of MDHAR from Oryza sativa (OsMDHAR) can prevent the deleterious effects of unfavorable growth conditions, we constructed a transgenic yeast strain harboring a recombinant plasmid carrying OsMDHAR (p426GPD::OsMDHAR).
- These results suggest that heterologous OsMDHAR expression increases tolerance to reactive oxygen species-induced oxidative stress by improving cellular redox homeostasis and improves survival during fermentation, which enhances fermentative capacity.
Functional Keywords
Literature and News
- Potential Application of the Oryza sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces cerevisiae . DOI: 10.1371/journal.pone.0158841 ; PMID: 27392090
- Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica . DOI: 10.1038/srep33903 ; PMID: 27652777
Gene Resources
- PANTHER: PTHR43557, PTHR43557:SF5
- SUPFAM: SSF51905, SSF55424
- Gene3D: 3.30.390.30, 3.50.50.60
Sequences
cDNA Sequence
- >LOC_Os09g39380.1
ATGGCGTCGGAGAAGCACTTCAAGTACGTCATCCTCGGCGGCGGCGTCGCAGCGGGATATGCGGCACGGGAGTTCGCCAAGCAGGGTGTTAAGCCAGGGGAGCTCGCCATCATCTCCAAGGAGGCCGTACTTTTACTTACTGGAACCATACCTGTTCTTGTTGTGGCTCCTTATGAGCGCCCTGCTCTCAGCAAGGGATACCTCTTTCCTCAGAATGCTGCAAGACTCCCAGGATTTCATGTGTGTGTCGGCAGTGGAGGAGAGAGGCTTTTGCCCGAATGGTACTCAGAGAAAGGCATTGAGTTGATCCTGAGCACTGAAATTGTCAAAGCTGATCTTGCCTCCAAGACTTTGACTAGTGCAGTTGGAGCAACCTTTACATATGAGATTTTGATCATTGCAACTGGCTCCTCAGTCATCAAGCTATCAGATTTTGGTACTCAAGGAGCTGATTCCAACAACATTTTATATCTAAGGGAAGTAGATGATGCTGACAAGCTGGTTGCAGCTATCCAAGCAAAGAAGGGTGGAAAGGCAGTTATTGTAGGAGGAGGTTACATTGGTCTAGAACTTAGTGCTGCATTGAAGATCAATGATTTTGATGTCACGATGGTGTTTCCTGAACCTTGGTGCATGCCTCGTCTCTTCACTGCTGATATCGCGGCTTTCTATGAGAGTTACTACACTAACAAAGGAGTTAAGATCGTGAAGGGTACAGTAGCTGTTGGTTTTGATGCTGATGCTAATGGTGATGTCACAGCAGTTAACCTGAAGAATGGCAGTGTGCTTGAAGCTGATATTGTTGTTGTTGGTGTTGGGGGCAGACCGCTGACTACTCTCTTTAAAGGTCAAGTTGCTGAGGAGAAAGGTGGAATTAAGACCGATGCTTTCTTTGAAACAAGTGTTCCTGGAGTCTATGCTGTCGGTGATGTGGCCACCTTCCCCATGAAGATGTACAATGAGTTGAGGAGAGTGGAACATGTTGACCATGCTAGGAAGTCTGCAGAGCAGGCTGTAAAGGCAATCAAGGGAAAAGAGTCCGGCGAGTCCGTTGTGGAGTATGACTATCTGCCATACTTCTACTCCCGGTCATTCGACCTGGGATGGCAATTCTACGGCGACAACGTTGGCGACACCATCCTGTTCGGAGACAGTGACCCGACCTCTGCCAAGCCCAAGTTCGGGTCCTACTGGATCAAGGACGGCAAGGTGTTGGGTGCCTTCCTGGAGGGTGGGTCACCTGATGAGAACAAGGCCATTGCCAAGGTCGCGAAAACTCAGCCGCCCGTTGCCAACATAGAGGAGCTCAAGAAGGAAGGCCTCCAGTTCGCCAGCAAAATATGA
CDS Sequence
- >LOC_Os09g39380.1
ATGGCGTCGGAGAAGCACTTCAAGTACGTCATCCTCGGCGGCGGCGTCGCAGCGGGATATGCGGCACGGGAGTTCGCCAAGCAGGGTGTTAAGCCAGGGGAGCTCGCCATCATCTCCAAGGAGGCCGTACTTTTACTTACTGGAACCATACCTGTTCTTGTTGTGGCTCCTTATGAGCGCCCTGCTCTCAGCAAGGGATACCTCTTTCCTCAGAATGCTGCAAGACTCCCAGGATTTCATGTGTGTGTCGGCAGTGGAGGAGAGAGGCTTTTGCCCGAATGGTACTCAGAGAAAGGCATTGAGTTGATCCTGAGCACTGAAATTGTCAAAGCTGATCTTGCCTCCAAGACTTTGACTAGTGCAGTTGGAGCAACCTTTACATATGAGATTTTGATCATTGCAACTGGCTCCTCAGTCATCAAGCTATCAGATTTTGGTACTCAAGGAGCTGATTCCAACAACATTTTATATCTAAGGGAAGTAGATGATGCTGACAAGCTGGTTGCAGCTATCCAAGCAAAGAAGGGTGGAAAGGCAGTTATTGTAGGAGGAGGTTACATTGGTCTAGAACTTAGTGCTGCATTGAAGATCAATGATTTTGATGTCACGATGGTGTTTCCTGAACCTTGGTGCATGCCTCGTCTCTTCACTGCTGATATCGCGGCTTTCTATGAGAGTTACTACACTAACAAAGGAGTTAAGATCGTGAAGGGTACAGTAGCTGTTGGTTTTGATGCTGATGCTAATGGTGATGTCACAGCAGTTAACCTGAAGAATGGCAGTGTGCTTGAAGCTGATATTGTTGTTGTTGGTGTTGGGGGCAGACCGCTGACTACTCTCTTTAAAGGTCAAGTTGCTGAGGAGAAAGGTGGAATTAAGACCGATGCTTTCTTTGAAACAAGTGTTCCTGGAGTCTATGCTGTCGGTGATGTGGCCACCTTCCCCATGAAGATGTACAATGAGTTGAGGAGAGTGGAACATGTTGACCATGCTAGGAAGTCTGCAGAGCAGGCTGTAAAGGCAATCAAGGGAAAAGAGTCCGGCGAGTCCGTTGTGGAGTATGACTATCTGCCATACTTCTACTCCCGGTCATTCGACCTGGGATGGCAATTCTACGGCGACAACGTTGGCGACACCATCCTGTTCGGAGACAGTGACCCGACCTCTGCCAAGCCCAAGTTCGGGTCCTACTGGATCAAGGACGGCAAGGTGTTGGGTGCCTTCCTGGAGGGTGGGTCACCTGATGAGAACAAGGCCATTGCCAAGGTCGCGAAAACTCAGCCGCCCGTTGCCAACATAGAGGAGCTCAAGAAGGAAGGCCTCCAGTTCGCCAGCAAAATATGA
Protein Sequence
- >LOC_Os09g39380.1
MASEKHFKYVILGGGVAAGYAAREFAKQGVKPGELAIISKEAVLLLTGTIPVLVVAPYERPALSKGYLFPQNAARLPGFHVCVGSGGERLLPEWYSEKGIELILSTEIVKADLASKTLTSAVGATFTYEILIIATGSSVIKLSDFGTQGADSNNILYLREVDDADKLVAAIQAKKGGKAVIVGGGYIGLELSAALKINDFDVTMVFPEPWCMPRLFTADIAAFYESYYTNKGVKIVKGTVAVGFDADANGDVTAVNLKNGSVLEADIVVVGVGGRPLTTLFKGQVAEEKGGIKTDAFFETSVPGVYAVGDVATFPMKMYNELRRVEHVDHARKSAEQAVKAIKGKESGESVVEYDYLPYFYSRSFDLGWQFYGDNVGDTILFGDSDPTSAKPKFGSYWIKDGKVLGAFLEGGSPDENKAIAKVAKTQPPVANIEELKKEGLQFASKI*