Information report for OsUgp1;Ugp1
Gene Details
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Functional Descriptions
- The suppression of UGPase by the introduction of a UGPase1-RNAi construct in wild-type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms-h mutant phenotype, whereas overexpression of UGPase1 in ms-h mutant plants restored male fertility and the transformants produced T(1) seeds that segregated into normal and chalky endosperms.
- Our results suggest that UGPase1 plays a key role in pollen development as well as seed carbohydrate metabolism.
- OsUgp1 has recently been reported to be essential for callose deposition during pollen mother cell and meiosis stages as well as for seed carbohydrate metabolism.
- OsUgp2 complements OsUgp1 to fulfill the UGPase’s functions necessary for the full process of pollen development.
- Pollen mother cells (PMCs) of Ugp1-silenced plants appeared normal before meiosis, but during meiosis, normal callose deposition was disrupted.
- These results demonstrate that rice Ugp1 is required for callose deposition during PMC meiosis and bridges the apoplastic unloading pathway and pollen development.
- We report a functional characterization of rice Ugp1, which is expressed throughout the plant, with highest expression in florets, especially in pollen during anther development.
- Expressing a double-stranded RNA interference construct in Ugp1-RI plants resulted in complete suppression of both Ugp1 and Ugp2, together with various pleiotropic developmental abnormalities, suggesting that UGPase plays critical roles in plant growth and development.
- In addition, both phenotypes were co-segregated with the UGPase1 transgene in segregating T(1) plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm.
- Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.).
- Ugp1 silencing by RNA interference or cosuppression results in male sterility.
Functional Keywords
Literature and News
- UDP-glucose pyrophosphorylase2 (OsUgp2), a pollen-preferential gene in rice, plays a critical role in starch accumulation during pollen maturation . DOI: 10.1007/s11434-008-0568-y
- Rice UDP-glucose pyrophosphorylase1 is essential for pollen callose deposition and its cosuppression results in a new type of thermosensitive genic male sterility . DOI: 10.1105/tpc.106.044123 ; PMID: 17400897
- Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.) . DOI: 10.1111/j.1365-313X.2008.03405.x ; PMID: 18182026
- Cloning of cDNA for UDP-glucose pyrophosphorylase and the expression of mRNA in rice endosperm . DOI: 10.1007/s00122-002-0927-z ; PMID: 12582522
Gene Resources
- UniProt: Q93X08
- EMBL: AB062606, AK065780, AK104344
- AlphaFoldDB: Q93X08
- EnsemblPlants: Os09t0553200-01
- Gramene: Os09t0553200-01
- KEGG: osa:4347800
- Orthologous matrix: KEYCFLS
- InterPro: IPR002618, IPR016267, IPR029044
- PANTHER: PTHR43511
- SUPFAM: SSF53448
- Gene3D: 2.160.10.10, 3.90.550.10
- OrthoDB: Q93X08
- SWISS-MODEL: Q93X08
- Conserved Domain Database: cd00897
- eggNOG: KOG2638
Sequences
cDNA Sequence
- >LOC_Os09g38030.1
CTCGCACACACACAACGCTCGCACTCCTCTCCTCTCATCTGACATATCTCCTCCCGTCCTTTCGAAGCCTCTCGAATCGCATCGCCGAGCCGGATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGAGGCTGCTCACGGAAACCTTCCAGATATATTTAGCTGAGCGTTTTGTAATTGTCGTGTGCATTCGGCAGCCAGGGCTCGATGATCCCATTACAGAATAATTGTAATCATCTTGGCACATCCGTACTTCTGTTTTTTGGTGTGCCAAGGGCGTTGTATTTCATTTACTTAAATGATCTCGTAATACCATAGTTTTGCATGCACTGTAGCTGGGAGCTCGTAGTTTACTGGTAATTCTATTCAGAGTTTACTACTGAT - >LOC_Os09g38030.2
CTCGCACACACACAACGCTCGCACTCCTCTCCTCTCATCTGACATATCTCCTCCCGTCCTTTCGAAGCCTCTCGAATCGCATCGCCGAGCCGGATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGAGGCTGCTCACGGAAACCTTCCAGATATATTTAGCTGAGCGTTTTGTAATTGTCGTGTGCATTCGGCAGCCAGGGCTCGATGATCCCATTACAGAATAATTGTAATCATCTTGGCACATCCGTACTTCTGTTTTTTGGTGTGCCAAGGGCGTTGTATTTCATTTACTTAAATGATCTCGTAATACCATAGTTTTGCATGCACTGTAGCTGGGAGCTCGTAGTTTACTGGTAATTCTATTCAGAGTTTACTACTGAT
CDS Sequence
- >LOC_Os09g38030.1
ATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGA - >LOC_Os09g38030.2
ATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGA
Protein Sequence
- >LOC_Os09g38030.1
MAVTADVKLEGLRAATDKLDQISENEKSGFISLVSRYLSGEAEQIEWSKIQTPTDEVVVPYDTLSAAPEDLNETKKLLDKLVVLKLNGGLGTTMGCTGPKSVIEVRNGFTFLDLIVIQIESLNKKYGCNVPLLLMNSFNTHDDTQKIVEKYSNSNIEIHTFNQSQYPRIVTEDFLPLPSKGKTGKDGWYPPGHGDVFPSLNNSGKLDTLLAQGKEYVFVANSDNLGAIVDIKILNHLIHNQNEYCMEVTPKTLADVKGGTLISYEGRVQLLEIAQVPDEHVNEFKSIEKFKIFNTNNLWVNLKAIKRLVEAEALKMEIIPNPKEVDGVKVLQLETAAGAAIRFFEKAIGINVPRSRFLPVKATSDLLLVQSDLYTLVDGFVIRNPARTNPSNPSIELGPEFKKVANFLARFKSIPSIVELDTLKVSGDVWFGSGVTLKGKVTITAKSGKLEIPDGAVLENKDINGPEDL* - >LOC_Os09g38030.2
MAVTADVKLEGLRAATDKLDQISENEKSGFISLVSRYLSGEAEQIEWSKIQTPTDEVVVPYDTLSAAPEDLNETKKLLDKLVVLKLNGGLGTTMGCTGPKSVIEVRNGFTFLDLIVIQIESLNKKYGCNVPLLLMNSFNTHDDTQKIVEKYSNSNIEIHTFNQSQYPRIVTEDFLPLPSKGKTGKDGWYPPGHGDVFPSLNNSGKLDTLLAQGKEYVFVANSDNLGAIVDIKILNHLIHNQNEYCMEVTPKTLADVKGGTLISYEGRVQLLEIAQVPDEHVNEFKSIEKFKIFNTNNLWVNLKAIKRLVEAEALKMEIIPNPKEVDGVKVLQLETAAGAAIRFFEKAIGINVPRSRFLPVKATSDLLLVQSDLYTLVDGFVIRNPARTNPSNPSIELGPEFKKVANFLARFKSIPSIVELDTLKVSGDVWFGSGVTLKGKVTITAKSGKLEIPDGAVLENKDINGPEDL*