Gene Details:

Functional Descriptions:

  • The suppression of UGPase by the introduction of a UGPase1-RNAi construct in wild-type plants nearly eliminated seed set because of the male defect, with developmental retardation similar to the ms-h mutant phenotype, whereas overexpression of UGPase1 in ms-h mutant plants restored male fertility and the transformants produced T(1) seeds that segregated into normal and chalky endosperms.
  • Our results suggest that UGPase1 plays a key role in pollen development as well as seed carbohydrate metabolism.
  • OsUgp1 has recently been reported to be essential for callose deposition during pollen mother cell and meiosis stages as well as for seed carbohydrate metabolism.
  • OsUgp2 complements OsUgp1 to fulfill the UGPase’s functions necessary for the full process of pollen development.
  • Pollen mother cells (PMCs) of Ugp1-silenced plants appeared normal before meiosis, but during meiosis, normal callose deposition was disrupted.
  • These results demonstrate that rice Ugp1 is required for callose deposition during PMC meiosis and bridges the apoplastic unloading pathway and pollen development.
  • We report a functional characterization of rice Ugp1, which is expressed throughout the plant, with highest expression in florets, especially in pollen during anther development.
  • Expressing a double-stranded RNA interference construct in Ugp1-RI plants resulted in complete suppression of both Ugp1 and Ugp2, together with various pleiotropic developmental abnormalities, suggesting that UGPase plays critical roles in plant growth and development.
  • In addition, both phenotypes were co-segregated with the UGPase1 transgene in segregating T(1) plants, which demonstrates that UGPase1 has functional roles in both male sterility and the development of a chalky endosperm.
  • Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.).
  • Ugp1 silencing by RNA interference or cosuppression results in male sterility.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os09g38030.1
    CTCGCACACACACAACGCTCGCACTCCTCTCCTCTCATCTGACATATCTCCTCCCGTCCTTTCGAAGCCTCTCGAATCGCATCGCCGAGCCGGATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGAGGCTGCTCACGGAAACCTTCCAGATATATTTAGCTGAGCGTTTTGTAATTGTCGTGTGCATTCGGCAGCCAGGGCTCGATGATCCCATTACAGAATAATTGTAATCATCTTGGCACATCCGTACTTCTGTTTTTTGGTGTGCCAAGGGCGTTGTATTTCATTTACTTAAATGATCTCGTAATACCATAGTTTTGCATGCACTGTAGCTGGGAGCTCGTAGTTTACTGGTAATTCTATTCAGAGTTTACTACTGAT
  • >LOC_Os09g38030.2
    CTCGCACACACACAACGCTCGCACTCCTCTCCTCTCATCTGACATATCTCCTCCCGTCCTTTCGAAGCCTCTCGAATCGCATCGCCGAGCCGGATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGAGGCTGCTCACGGAAACCTTCCAGATATATTTAGCTGAGCGTTTTGTAATTGTCGTGTGCATTCGGCAGCCAGGGCTCGATGATCCCATTACAGAATAATTGTAATCATCTTGGCACATCCGTACTTCTGTTTTTTGGTGTGCCAAGGGCGTTGTATTTCATTTACTTAAATGATCTCGTAATACCATAGTTTTGCATGCACTGTAGCTGGGAGCTCGTAGTTTACTGGTAATTCTATTCAGAGTTTACTACTGAT
CDS Sequence
  • >LOC_Os09g38030.1
    ATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGA
  • >LOC_Os09g38030.2
    ATGGCGGTCACCGCCGACGTGAAGCTCGAGGGCCTCCGCGCCGCCACCGACAAGCTCGACCAGATCAGCGAGAACGAGAAGTCCGGGTTCATCAGCCTCGTTTCGCGCTACCTCAGCGGCGAGGCGGAGCAGATCGAGTGGAGTAAGATCCAGACCCCGACCGACGAGGTGGTGGTTCCCTACGACACGCTCTCGGCTGCTCCCGAAGATCTCAACGAGACGAAGAAGCTGCTCGACAAACTCGTCGTGCTCAAGCTCAATGGAGGCCTCGGGACGACCATGGGCTGCACTGGTCCCAAGTCTGTCATTGAAGTCCGCAATGGCTTTACGTTTCTAGACCTTATTGTGATTCAAATTGAGTCCCTGAACAAGAAGTATGGATGCAATGTCCCTTTGCTTCTAATGAACTCATTCAACACTCATGATGACACACAGAAGATTGTTGAGAAGTACTCCAACTCCAACATTGAAATTCACACTTTCAACCAGAGCCAATATCCTCGCATTGTTACTGAAGACTTCTTGCCACTTCCAAGCAAGGGGAAGACTGGCAAGGATGGCTGGTATCCCCCAGGCCATGGTGATGTGTTCCCCTCCTTGAATAACAGTGGAAAACTTGATACCTTGTTGGCACAGGGCAAGGAGTATGTCTTTGTCGCGAACTCGGACAACTTGGGTGCTATTGTTGACATCAAGATCTTAAACCACCTGATCCATAACCAGAACGAGTACTGTATGGAGGTTACTCCTAAAACATTGGCTGATGTTAAAGGTGGTACCCTCATCTCTTACGAAGGGAGAGTTCAGCTGTTGGAGATTGCTCAAGTCCCTGATGAGCATGTGAATGAATTCAAGTCAATTGAGAAGTTCAAGATCTTCAATACCAACAACCTGTGGGTGAACTTGAAGGCCATCAAGAGGCTGGTAGAAGCTGAAGCACTTAAGATGGAAATCATTCCTAACCCTAAGGAAGTTGATGGTGTGAAAGTTCTGCAACTCGAAACTGCAGCTGGAGCAGCCATTCGGTTCTTTGAAAAAGCAATTGGCATTAATGTTCCCCGCTCGAGATTTCTGCCAGTCAAGGCTACATCTGATTTGTTGCTTGTACAGTCTGATTTGTACACCTTGGTCGATGGCTTTGTCATCCGCAACCCAGCCAGAACAAACCCATCAAACCCTTCGATTGAGCTTGGACCTGAGTTCAAGAAGGTTGCCAATTTCCTGGCCCGGTTTAAGTCAATCCCCAGCATTGTCGAGCTTGACACCTTGAAGGTCTCTGGTGATGTCTGGTTCGGCTCTGGAGTTACACTCAAGGGTAAGGTGACCATCACCGCCAAGTCAGGGAAGTTGGAGATCCCAGATGGAGCCGTGCTTGAGAACAAGGACATCAACGGTCCGGAGGATCTTTGA
Protein Sequence
  • >LOC_Os09g38030.1
    MAVTADVKLEGLRAATDKLDQISENEKSGFISLVSRYLSGEAEQIEWSKIQTPTDEVVVPYDTLSAAPEDLNETKKLLDKLVVLKLNGGLGTTMGCTGPKSVIEVRNGFTFLDLIVIQIESLNKKYGCNVPLLLMNSFNTHDDTQKIVEKYSNSNIEIHTFNQSQYPRIVTEDFLPLPSKGKTGKDGWYPPGHGDVFPSLNNSGKLDTLLAQGKEYVFVANSDNLGAIVDIKILNHLIHNQNEYCMEVTPKTLADVKGGTLISYEGRVQLLEIAQVPDEHVNEFKSIEKFKIFNTNNLWVNLKAIKRLVEAEALKMEIIPNPKEVDGVKVLQLETAAGAAIRFFEKAIGINVPRSRFLPVKATSDLLLVQSDLYTLVDGFVIRNPARTNPSNPSIELGPEFKKVANFLARFKSIPSIVELDTLKVSGDVWFGSGVTLKGKVTITAKSGKLEIPDGAVLENKDINGPEDL*
  • >LOC_Os09g38030.2
    MAVTADVKLEGLRAATDKLDQISENEKSGFISLVSRYLSGEAEQIEWSKIQTPTDEVVVPYDTLSAAPEDLNETKKLLDKLVVLKLNGGLGTTMGCTGPKSVIEVRNGFTFLDLIVIQIESLNKKYGCNVPLLLMNSFNTHDDTQKIVEKYSNSNIEIHTFNQSQYPRIVTEDFLPLPSKGKTGKDGWYPPGHGDVFPSLNNSGKLDTLLAQGKEYVFVANSDNLGAIVDIKILNHLIHNQNEYCMEVTPKTLADVKGGTLISYEGRVQLLEIAQVPDEHVNEFKSIEKFKIFNTNNLWVNLKAIKRLVEAEALKMEIIPNPKEVDGVKVLQLETAAGAAIRFFEKAIGINVPRSRFLPVKATSDLLLVQSDLYTLVDGFVIRNPARTNPSNPSIELGPEFKKVANFLARFKSIPSIVELDTLKVSGDVWFGSGVTLKGKVTITAKSGKLEIPDGAVLENKDINGPEDL*