Gene Details:

Functional Descriptions:

  • OsMLH3 and OsMLH1 (MutL-homolog 1) interact to form a heterodimer (MutL) to promote crossover formation in the macrospore and microspore mother cells and development of functional megaspore during meiosis, defective OsMLH3 or OsMLH1 in fsv1 and CRISPR/Cas9-based knockout lines results in reduced type I crossover and bivalent frequency.
  • Through map-based cloning and functional analyses, we isolated the causal gene of fsv1, OsMLH3 encoding a MutL-homolog 3 protein, an ortholog of HvMLH3 in barley and AtMLH3 in Arabidopsis.
  • Mutation in OsMLH3 also affects the pollen fertility.
  • OsMLH1 interacts with OsMLH3 to regulate synapsis and interference-sensitive crossover formation during meiosis in rice.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os09g37930.1
    ATGCCGTCCTGGCGCGCGTGGAGGTCGGCGGCGGCGACACAGATGGTCTGGTGGTCGGCGGAGGTCCCGGCGCGCGCGTTGGTCGGCGGCGGCGACACGGATGGCGGCGTCGATGGCGTCTTCCAGGGCGCTGCCGCGCTGCTCGCCTTCACGCGGACCGGCGGCGCTCCGGTCGGGGGGCAGCGCCGACCACGCCCACCGCTGCCGCCGGAGCTCGCCTGCTCCACTGCTCACCCGCGCCGTCCTCTGCCCGGGAAGAAGAGAGAGAAGGGAGAGGGAAGAAGAGAGGAGGGGAAACTGATCGTTTGTTGGGTTCGTGGTGGCTACTCCATCAATACAATAAAACGGTTGCCAAAAAGTGTCCATAGCTCGTTGCGCTCAAGCATTGTTCTATTTGACCTATCAAGGGTTGTTGAGGAGCTGGTATATAATAGCATTGACGCAAATGCGAGCAAGATTGACATCTCAGTGAATGCCAGAGCATGTTATGTTAAAGTGGAAGATGATGGCTGTGGTATTACTCGTGACGAACTGGTTCTTGTAGGAGAAAAATATGCAACATCCAAGTTTCATAATGTCATGGTTGATGGGGAACCTAGTTCCAGAAGTTTTGGATTAAATGGCGAAGCACTCGCATCACTATCTGATATCTCTGTTGTTGAAGTCAGGACAAAAGCTCGCGGGCGACCAAATTCATATTGCAAGATAATAAAGGGATCCAAATGCTCACACCTGGGAATAGATGAGCAGAGGGAAGTTGTTGGAACCACAGTTATTGTTCGCGAGCTTTTTTACAATCAACCTGTACGCAGGAAACAAATGCAATCTAGTTACAAAAGAGAACTACATCTTGTGAAGAAGTCTGTTCTGCGAGTTGCACTCATTCATCCACAAGTTTCACTCAGACTTTTTGATATTGAGAGTGAAGATGAGTTGCTATACACGATTCCTTCATCCTCCCCCTTGCCTCTTGTATCAAACATTTTGGGGAAAAATGTCTCCAGCTGTCTTCATGAGATAGCTACCTCTGACAAGCATTTTGCTCTTTCAGGGCACATCTCCAGACCAACAGATGTGTTCTGTAATAAGGATTGGCAAACTGTCATGTATTTCTTTGAACGAACTATCACAGACTATTGGAAGAAGCATGCACCTCAACTGCCAGAAGTGAAAGCTATTGGCAATGATACCTGTGTGCCTTTGGAAAGAGATGTGAAATCAAGTCAGGAACTGTTAAGGCGTCATGGTGTGCAGAAGAAAGAAGATGTCGCTGAATTGTACCAAACAGCTCTGCAGAAGAATACAGTACGAGACATGAATTTTGATACAGCTGCCCCAGCAGAACCTAAAGACAATTACCTTTCTTTGGATATGGAGCCATCCACATGGCGTGCCTGCTATGACCAGATCAGTGATGCATCCCACACAGATGATGTTGCTAGGAATGGTCGGAAATTTGGTCATAAGCAAATATGTTCCCTTCAAAGTTATAGTTATCAGTGGTTAGAGGACGGCTCTTCCCTGTTAGAAGACTCTGATCTTTCAAGTGCTAACCCAACTATTTGTAAAATGCAAAAGACAGAAGATATATTCCATGGGCATGCATATTCTGGTAAGTTTGGACTGCTACAAGATGCAGAAATCGAAATCGGTCCAGAAATTAAACTCCAAGAATATTGCTTTGAATCTCCCAATAAACCGAACAGAATGACCTGCGATTTTGTGCAAAAGCAAACCAATATAGAGGCACACATTTCAGGCCGTGATGGATTCTATGTTGATTTTGATAAATTGAACGAGGACTGTCTACTCAATGAGATATCAAAGACAATCACTGATGTTTCCTGCCCTCAAATGCCACACTTTAATGATGGACTCTGTCCTGAGGACGTTGGCTCCTCCAAGAGTTCCTGTAGTGTCAGGAAAAAGTCTAGTAAAAGGCAAAATAGTGCTAATGCAATTGCCCAGATGAAGTTCCATGATATGCAAGCAGTTTGCGAAAGTGATTACATGGATAGGTCCTTCATCAAGGATACATGTGGCCTTCATTTCTTTCATCCATTCTCGTTGGCTGATACACCTCGCAGTCACAGTCGTGCAAGGATTGACTTGGAATTGCATGGAAGGTCAAATGAAAGCATTAACAGTTGGAACTGTGAAAATATTGGCACTGATTTTGGATTTACTTCAGACAGGTTTAATATTGATTCATCAATGATTTTTGAAGGAAGCAAACATCTCAATAACTTTGGCAATGGAACTCAATCTCCTAGTTACTTCAATCATGAATATTGTTCTGTCGGTCAGTTTGCTTCCAAACAGGATCGGATACCCTTGAAATCAAAACATGATGCAAGAATGTCATATGATATTTCTCCTGAGAAAAGTTCTACTGGTTGTCATTTGAATGTTTCTTTTTCCCAAGTGGCAAAAAGCAGCAAGCTTACTGAAGATCAGTATGGATGCAGTCAGAGGCCCAGGCTTTCCAGAGGTAGATATAGGAGTCGTTCTGCACCACCATTTTACAGAGGCAAAAGAAAATTCCCTAGATTAAACGAACCACTAACCAAATTGACTACAGAAGGTGGTAAATATACCACTGTTAATGACTCAGGAGATATCACACCTGTTCAGGAGTATACATCACATATGAACGCAACCCAACCTATTCCTGAGACCTTTAGCAATGACTTTTCTGACCTAAATTTCAGCTTGAAGGGAAATGTCAAAATGTGCGAAGAGAAATGTTCTGATGAACTCGAAGATTCTACTGCTTCAGATGAAATAACAAAATGGCGTGATGACTCTGACCATCATGCAGTTGAGCATTTTGTGCCAAGAGTTATATCACTGTGCACAATTTTCTATGCTCATATTTTATCTCTCTGTGAGGCAAGAGTTTTGTTGCAGCTGGATAAGAAATTTATTCCTGTCATATCTGGGGAAGTACTACTCCTTGTTGATCAGGAAGAATTCATTTTTTATGTTCACATACTATATGAAGTAGTCAGTGTGTATATGGTGACAAAGCATGCAGCTGATGAAAGGATACGTTTGGAGGAACTCCGTCGAAAGGTTTTATCAGATGATGGCAGAGGGATTACTTACTTGGACTCTGAGGAGGACTTAGTTCTCCCTGAGACTGGATTTCAATTGTTCCAAAAGTATATGCAACAGATCCAAAGTTGGGGCTGGATCATCAACAGTACTAATTCCTGTGAATCATTCAAAAAGAACATGAACGTTCTGAGGAGACAATCGCGCCGTCTTACTCTTGCTGCTGTTCCATGTATTTTGGGCGTCACTTTAACAGGAAAAGATCTTATGGACTTCATTCAGCAGCTTGATGACACAGATGGGTCGTCAGCTATCCCCCCAGCGGTCATTCGTATTCTCAATTTCAAGGCTTGCAGAGGTGCGATCATGTTTGGCGATCCTCTGCTACCATCGGAGTGCTCTCTGATTATCGAAGAACTGAAAGCAACATCTTTATGTTTCCAGTGTGCTCATGGACGTCCGACCACCGTGCCGATTGTGAACGTCGCATCTCTCCGCGGCGAGTTGGCGAGGCTCGGAGCGGTGAACGGAAGGCAAGAAGAGACCTGGCATGGTCTCTCGCACCATGGACCCAGCCTTGAACGTGCTCGAACGCGCCTCAGAGAACTGAGAAAGCTACGTGGTGGCCTGTAG
CDS Sequence
  • >LOC_Os09g37930.1
    ATGCCGTCCTGGCGCGCGTGGAGGTCGGCGGCGGCGACACAGATGGTCTGGTGGTCGGCGGAGGTCCCGGCGCGCGCGTTGGTCGGCGGCGGCGACACGGATGGCGGCGTCGATGGCGTCTTCCAGGGCGCTGCCGCGCTGCTCGCCTTCACGCGGACCGGCGGCGCTCCGGTCGGGGGGCAGCGCCGACCACGCCCACCGCTGCCGCCGGAGCTCGCCTGCTCCACTGCTCACCCGCGCCGTCCTCTGCCCGGGAAGAAGAGAGAGAAGGGAGAGGGAAGAAGAGAGGAGGGGAAACTGATCGTTTGTTGGGTTCGTGGTGGCTACTCCATCAATACAATAAAACGGTTGCCAAAAAGTGTCCATAGCTCGTTGCGCTCAAGCATTGTTCTATTTGACCTATCAAGGGTTGTTGAGGAGCTGGTATATAATAGCATTGACGCAAATGCGAGCAAGATTGACATCTCAGTGAATGCCAGAGCATGTTATGTTAAAGTGGAAGATGATGGCTGTGGTATTACTCGTGACGAACTGGTTCTTGTAGGAGAAAAATATGCAACATCCAAGTTTCATAATGTCATGGTTGATGGGGAACCTAGTTCCAGAAGTTTTGGATTAAATGGCGAAGCACTCGCATCACTATCTGATATCTCTGTTGTTGAAGTCAGGACAAAAGCTCGCGGGCGACCAAATTCATATTGCAAGATAATAAAGGGATCCAAATGCTCACACCTGGGAATAGATGAGCAGAGGGAAGTTGTTGGAACCACAGTTATTGTTCGCGAGCTTTTTTACAATCAACCTGTACGCAGGAAACAAATGCAATCTAGTTACAAAAGAGAACTACATCTTGTGAAGAAGTCTGTTCTGCGAGTTGCACTCATTCATCCACAAGTTTCACTCAGACTTTTTGATATTGAGAGTGAAGATGAGTTGCTATACACGATTCCTTCATCCTCCCCCTTGCCTCTTGTATCAAACATTTTGGGGAAAAATGTCTCCAGCTGTCTTCATGAGATAGCTACCTCTGACAAGCATTTTGCTCTTTCAGGGCACATCTCCAGACCAACAGATGTGTTCTGTAATAAGGATTGGCAAACTGTCATGTATTTCTTTGAACGAACTATCACAGACTATTGGAAGAAGCATGCACCTCAACTGCCAGAAGTGAAAGCTATTGGCAATGATACCTGTGTGCCTTTGGAAAGAGATGTGAAATCAAGTCAGGAACTGTTAAGGCGTCATGGTGTGCAGAAGAAAGAAGATGTCGCTGAATTGTACCAAACAGCTCTGCAGAAGAATACAGTACGAGACATGAATTTTGATACAGCTGCCCCAGCAGAACCTAAAGACAATTACCTTTCTTTGGATATGGAGCCATCCACATGGCGTGCCTGCTATGACCAGATCAGTGATGCATCCCACACAGATGATGTTGCTAGGAATGGTCGGAAATTTGGTCATAAGCAAATATGTTCCCTTCAAAGTTATAGTTATCAGTGGTTAGAGGACGGCTCTTCCCTGTTAGAAGACTCTGATCTTTCAAGTGCTAACCCAACTATTTGTAAAATGCAAAAGACAGAAGATATATTCCATGGGCATGCATATTCTGGTAAGTTTGGACTGCTACAAGATGCAGAAATCGAAATCGGTCCAGAAATTAAACTCCAAGAATATTGCTTTGAATCTCCCAATAAACCGAACAGAATGACCTGCGATTTTGTGCAAAAGCAAACCAATATAGAGGCACACATTTCAGGCCGTGATGGATTCTATGTTGATTTTGATAAATTGAACGAGGACTGTCTACTCAATGAGATATCAAAGACAATCACTGATGTTTCCTGCCCTCAAATGCCACACTTTAATGATGGACTCTGTCCTGAGGACGTTGGCTCCTCCAAGAGTTCCTGTAGTGTCAGGAAAAAGTCTAGTAAAAGGCAAAATAGTGCTAATGCAATTGCCCAGATGAAGTTCCATGATATGCAAGCAGTTTGCGAAAGTGATTACATGGATAGGTCCTTCATCAAGGATACATGTGGCCTTCATTTCTTTCATCCATTCTCGTTGGCTGATACACCTCGCAGTCACAGTCGTGCAAGGATTGACTTGGAATTGCATGGAAGGTCAAATGAAAGCATTAACAGTTGGAACTGTGAAAATATTGGCACTGATTTTGGATTTACTTCAGACAGGTTTAATATTGATTCATCAATGATTTTTGAAGGAAGCAAACATCTCAATAACTTTGGCAATGGAACTCAATCTCCTAGTTACTTCAATCATGAATATTGTTCTGTCGGTCAGTTTGCTTCCAAACAGGATCGGATACCCTTGAAATCAAAACATGATGCAAGAATGTCATATGATATTTCTCCTGAGAAAAGTTCTACTGGTTGTCATTTGAATGTTTCTTTTTCCCAAGTGGCAAAAAGCAGCAAGCTTACTGAAGATCAGTATGGATGCAGTCAGAGGCCCAGGCTTTCCAGAGGTAGATATAGGAGTCGTTCTGCACCACCATTTTACAGAGGCAAAAGAAAATTCCCTAGATTAAACGAACCACTAACCAAATTGACTACAGAAGGTGGTAAATATACCACTGTTAATGACTCAGGAGATATCACACCTGTTCAGGAGTATACATCACATATGAACGCAACCCAACCTATTCCTGAGACCTTTAGCAATGACTTTTCTGACCTAAATTTCAGCTTGAAGGGAAATGTCAAAATGTGCGAAGAGAAATGTTCTGATGAACTCGAAGATTCTACTGCTTCAGATGAAATAACAAAATGGCGTGATGACTCTGACCATCATGCAGTTGAGCATTTTGTGCCAAGAGTTATATCACTGTGCACAATTTTCTATGCTCATATTTTATCTCTCTGTGAGGCAAGAGTTTTGTTGCAGCTGGATAAGAAATTTATTCCTGTCATATCTGGGGAAGTACTACTCCTTGTTGATCAGGAAGAATTCATTTTTTATGTTCACATACTATATGAAGTAGTCAGTGTGTATATGGTGACAAAGCATGCAGCTGATGAAAGGATACGTTTGGAGGAACTCCGTCGAAAGGTTTTATCAGATGATGGCAGAGGGATTACTTACTTGGACTCTGAGGAGGACTTAGTTCTCCCTGAGACTGGATTTCAATTGTTCCAAAAGTATATGCAACAGATCCAAAGTTGGGGCTGGATCATCAACAGTACTAATTCCTGTGAATCATTCAAAAAGAACATGAACGTTCTGAGGAGACAATCGCGCCGTCTTACTCTTGCTGCTGTTCCATGTATTTTGGGCGTCACTTTAACAGGAAAAGATCTTATGGACTTCATTCAGCAGCTTGATGACACAGATGGGTCGTCAGCTATCCCCCCAGCGGTCATTCGTATTCTCAATTTCAAGGCTTGCAGAGGTGCGATCATGTTTGGCGATCCTCTGCTACCATCGGAGTGCTCTCTGATTATCGAAGAACTGAAAGCAACATCTTTATGTTTCCAGTGTGCTCATGGACGTCCGACCACCGTGCCGATTGTGAACGTCGCATCTCTCCGCGGCGAGTTGGCGAGGCTCGGAGCGGTGAACGGAAGGCAAGAAGAGACCTGGCATGGTCTCTCGCACCATGGACCCAGCCTTGAACGTGCTCGAACGCGCCTCAGAGAACTGAGAAAGCTACGTGGTGGCCTGTAG
Protein Sequence
  • >LOC_Os09g37930.1
    MPSWRAWRSAAATQMVWWSAEVPARALVGGGDTDGGVDGVFQGAAALLAFTRTGGAPVGGQRRPRPPLPPELACSTAHPRRPLPGKKREKGEGRREEGKLIVCWVRGGYSINTIKRLPKSVHSSLRSSIVLFDLSRVVEELVYNSIDANASKIDISVNARACYVKVEDDGCGITRDELVLVGEKYATSKFHNVMVDGEPSSRSFGLNGEALASLSDISVVEVRTKARGRPNSYCKIIKGSKCSHLGIDEQREVVGTTVIVRELFYNQPVRRKQMQSSYKRELHLVKKSVLRVALIHPQVSLRLFDIESEDELLYTIPSSSPLPLVSNILGKNVSSCLHEIATSDKHFALSGHISRPTDVFCNKDWQTVMYFFERTITDYWKKHAPQLPEVKAIGNDTCVPLERDVKSSQELLRRHGVQKKEDVAELYQTALQKNTVRDMNFDTAAPAEPKDNYLSLDMEPSTWRACYDQISDASHTDDVARNGRKFGHKQICSLQSYSYQWLEDGSSLLEDSDLSSANPTICKMQKTEDIFHGHAYSGKFGLLQDAEIEIGPEIKLQEYCFESPNKPNRMTCDFVQKQTNIEAHISGRDGFYVDFDKLNEDCLLNEISKTITDVSCPQMPHFNDGLCPEDVGSSKSSCSVRKKSSKRQNSANAIAQMKFHDMQAVCESDYMDRSFIKDTCGLHFFHPFSLADTPRSHSRARIDLELHGRSNESINSWNCENIGTDFGFTSDRFNIDSSMIFEGSKHLNNFGNGTQSPSYFNHEYCSVGQFASKQDRIPLKSKHDARMSYDISPEKSSTGCHLNVSFSQVAKSSKLTEDQYGCSQRPRLSRGRYRSRSAPPFYRGKRKFPRLNEPLTKLTTEGGKYTTVNDSGDITPVQEYTSHMNATQPIPETFSNDFSDLNFSLKGNVKMCEEKCSDELEDSTASDEITKWRDDSDHHAVEHFVPRVISLCTIFYAHILSLCEARVLLQLDKKFIPVISGEVLLLVDQEEFIFYVHILYEVVSVYMVTKHAADERIRLEELRRKVLSDDGRGITYLDSEEDLVLPETGFQLFQKYMQQIQSWGWIINSTNSCESFKKNMNVLRRQSRRLTLAAVPCILGVTLTGKDLMDFIQQLDDTDGSSAIPPAVIRILNFKACRGAIMFGDPLLPSECSLIIEELKATSLCFQCAHGRPTTVPIVNVASLRGELARLGAVNGRQEETWHGLSHHGPSLERARTRLRELRKLRGGL*