Information report for OsPIP2;7
Gene Details
|
|
Functional Descriptions
- qRT-PCR analysis of PIPs in rice root and shoot tissues revealed a significant down regulation of transcripts encoding OsPIP1;2, OsPIP1;3, OsPIP2;4, OsPIP2;6, and OsPIP2;7 in response to arsenite treatment.
- Overexpression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Arabidopsis yielded enhanced arsenite tolerance and higher biomass accumulation.
- Transcript analysis demonstrated that the expression of OsPIP2;4 and OsPIP2;7 were downregulated in shoots and strongly upregulated in rice roots by high B treatment.
- Arabidopsis lines overexpressing OsPIP2;4 and OsPIP2;7 showed significantly higher biomass production and greater root length, however there was no difference in B accumulation in long term uptake assay.
- Short-term uptake assay using tracer B ((1)(0)B) in shoots and roots demonstrated increased (1)(0)B accumulation in Arabidopsis lines expressing OsPIP2;4 and OsPIP2;7, compare to wild type control plants.
- Efflux assay of B in the roots showed that (1)(0)B was effluxed from the Arabidopsis transgenic plants overexpressing OsPIP2;4 or OsPIP2;7 during the initial 1-h of assay.
- These data indicate that OsPIP2;4 and OsPIP2;7 are involved in mediating B transport in rice and provide tolerance via efflux of excess B from roots and shoot tissues.
- Two rice plasma membrane intrinsic proteins, OsPIP2;4 and OsPIP2;7, are involved in transport and providing tolerance to boron toxicity.
- We have previously shown that a rice gene, OsPIP2;7, was generally up-regulated in roots but down-regulated in shoots at the early stage of chilling stress.
- Heterologous expression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Xenopus laevis oocytes significantly increased the uptake of arsenite.
- Furthermore, OsPIP2;7 enhanced the transpiration rate and tolerance to low temperature when overexpressed in rice.
- These results indicated that OsPIP2;7 was involved in rapid water transport and maintenance of the water balance in cells, and ultimately improves the tolerance of yeast and rice to low temperature stress.
Literature and News
- Two rice plasma membrane intrinsic proteins, OsPIP2;4 and OsPIP2;7, are involved in transport and providing tolerance to boron toxicity . DOI: 10.1007/s00425-013-1969-y ; PMID: 24142111
- Characterization of OsPIP2;7, a water channel protein in rice . DOI: 10.1093/pcp/pcn166 ; PMID: 18988636
- Members of rice plasma membrane intrinsic proteins subfamily are involved in arsenite permeability and tolerance in plants . DOI: 10.1007/s11248-012-9600-8 ; PMID: 22350764
Gene Resources
- UniProt: Q651D5
- EMBL: AB109206, AK067792, AK104786
- AlphaFoldDB: Q651D5
- EnsemblPlants: Os09t0541000-01
- Gramene: Os09t0541000-01
- KEGG: dosa:Os09g0541000
- Orthologous matrix: AMCHLWA
- InterPro: IPR000425, IPR022357, IPR023271
Sequences
cDNA Sequence
- >LOC_Os09g36930.1
ATCGTCTTCGTGGTGATAAGGAGTAGGAGAGAAGGCCATGGCGTCGAAGGAGGAGGTGGCCGTGGAGACGGTGGAGGGCGGAGCGGCGGCGGCGAAGGCGCCGTACTGGGACCCGCCGCCGGCGCCGCTGCTGGACACGAGCGAGCTGGGTAAGTGGTCGCTGTACCGTGCCCTCATCGCGGAGTTCATGGCGACGCTCATCTTCCTCTACGTGAGCATCGCCACCGTGATCGGGTACAAGAACCAGAGGGCCACCGTCGACGCGTGCACCGGCGTCGGCTACCTCGGCGTGGCGTGGTCGTTCGGTGCCACCATATTCGTCCTCGTCTACTGCACCGGCGGCGTCTCCGGCGGGCACATCAACCCGGCGGTGACGCTGGGCCTCTTCTTCGGGCGGAAGCTGTCGCTCGTCCGCACCGTGCTGTACGTCGTGGCGCAGTGCCTCGGCGCCATCGCCGGCGCCGGCATCGTCAAGGGGATCATGAAGCGCCCCTACGACGCGCTCGGCGGCGGCGCCAACACCGTCAGCGACGGCTACTCCGCCGCCGGCGCCCTCGGCGCCGAGATCGTCGGCACGTTCATCCTCGTCTACACCGTCTTCTCCGCCACCGACCCCAAGCGCACCGCCCGCGACTCCTTCATCCCCGTACTCGTGCCGCTGCCGATCGGGTTCGCGGTGTTCGTCGTGCACCTGGCGACGATTCCGATCACCGGCACGGGCATCAACCCGGCGAGGAGCCTCGGCGCCGCCGTGCTGTACAACCAGCACGCAGCTTGGAAAGACCACTGGATCTTCTGGGTGGGGCCGGTGATCGGGGCGTTCTTGGCGGCGGCGTACCACAAGCTGGTGCTGCGCGGCGAGGCCGCCAAGGCGCTCAGCTCGTTCAGGAGCACCAGCGTGACGGCGTGAGGAAGACGACGATGTTCATTTGATACGAGAGATCGATCAGCTGCTTGATTAATTGTTCTTGATTTCGTTGTTCAATTTACAATCTACGACTATGGACGATCATGCATATGATATGATCGATGCATGCGTCTGTTGTCAGTTTTATTTGGGACTTTCAGTGTGAGAGTGTGAATGGTTGTAAAATAAGTGGAGTGCCTGTATGTATCTGTATGCATGCACGGCACGGTACGCGTGTTTATGGATGATGCTTTGTTACGTAGCAGGCTTAATTATGTCAATATGTGTGCGTATGTTGTCGTGGTAATTATTATACTGGGTATATATATATATTGTGCAGCTTAATTATCTAGCCATGTACAAATTTTCGTATGCGGAATAATATAATGCATGCGATCGCCATTCATTAGGT
CDS Sequence
- >LOC_Os09g36930.1
ATGGCGTCGAAGGAGGAGGTGGCCGTGGAGACGGTGGAGGGCGGAGCGGCGGCGGCGAAGGCGCCGTACTGGGACCCGCCGCCGGCGCCGCTGCTGGACACGAGCGAGCTGGGTAAGTGGTCGCTGTACCGTGCCCTCATCGCGGAGTTCATGGCGACGCTCATCTTCCTCTACGTGAGCATCGCCACCGTGATCGGGTACAAGAACCAGAGGGCCACCGTCGACGCGTGCACCGGCGTCGGCTACCTCGGCGTGGCGTGGTCGTTCGGTGCCACCATATTCGTCCTCGTCTACTGCACCGGCGGCGTCTCCGGCGGGCACATCAACCCGGCGGTGACGCTGGGCCTCTTCTTCGGGCGGAAGCTGTCGCTCGTCCGCACCGTGCTGTACGTCGTGGCGCAGTGCCTCGGCGCCATCGCCGGCGCCGGCATCGTCAAGGGGATCATGAAGCGCCCCTACGACGCGCTCGGCGGCGGCGCCAACACCGTCAGCGACGGCTACTCCGCCGCCGGCGCCCTCGGCGCCGAGATCGTCGGCACGTTCATCCTCGTCTACACCGTCTTCTCCGCCACCGACCCCAAGCGCACCGCCCGCGACTCCTTCATCCCCGTACTCGTGCCGCTGCCGATCGGGTTCGCGGTGTTCGTCGTGCACCTGGCGACGATTCCGATCACCGGCACGGGCATCAACCCGGCGAGGAGCCTCGGCGCCGCCGTGCTGTACAACCAGCACGCAGCTTGGAAAGACCACTGGATCTTCTGGGTGGGGCCGGTGATCGGGGCGTTCTTGGCGGCGGCGTACCACAAGCTGGTGCTGCGCGGCGAGGCCGCCAAGGCGCTCAGCTCGTTCAGGAGCACCAGCGTGACGGCGTGA
Protein Sequence
- >LOC_Os09g36930.1
MASKEEVAVETVEGGAAAAKAPYWDPPPAPLLDTSELGKWSLYRALIAEFMATLIFLYVSIATVIGYKNQRATVDACTGVGYLGVAWSFGATIFVLVYCTGGVSGGHINPAVTLGLFFGRKLSLVRTVLYVVAQCLGAIAGAGIVKGIMKRPYDALGGGANTVSDGYSAAGALGAEIVGTFILVYTVFSATDPKRTARDSFIPVLVPLPIGFAVFVVHLATIPITGTGINPARSLGAAVLYNQHAAWKDHWIFWVGPVIGAFLAAAYHKLVLRGEAAKALSSFRSTSVTA*