Gene Details:

Functional Descriptions:

  • The OsDREB1B gene was differentially regulated at the transcriptional level by osmotic stress, oxidative stress, salicylic acid, ABA, and cold.
  • Histochemical analysis of GUS expression in T(2) transgenic Arabidopsis plants indicated that OsDREB1B shows stress-specific induction pattern in response to a variety of stresses like mannitol, NaCl, PEG, methyl viologen, cold, ABA, and salicylic acid.
  • Seven zinc-finger transcription factors are novel regulators of the stress responsive gene OsDREB1B.
  • Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high-salt stresses.
  • However, here it is shown that OsDREB1B is not only induced by low temperatures, but also by drought and mechanical stress.
  • The data obtained provide strong in vivo evidence that OsDREB1B is involved in both abiotic and biotic stress responses, and confers broad-spectrum stress tolerance to transgenic plants.
  • Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L.
  • Results indicated that the OsDREB1BI gene was over-expressed in transgenic plants under cold and high-temperature, meanwhile, those transgenic plants also revealed freezing and heat tolerance.
  • Overexpression of OsDREB1B in tobacco also improved the oxidative and freezing stress tolerance of transgenic plants.
  • Under salt stress treatment, the expression levels of OsbZIP05, OsHKT1;1 and OsDREB1B were significantly lower yet the level of OsHKT2;1 was significantly higher in oswrky28 mutants than those in wide type plants.
  • OsWRKY28 confers salinity tolerance by directly binding to OsDREB1B promoter and increasing its transcriptional activity, and negatively regulates abscisic acid mediated seedling establishment in rice.
  • OsWRKY28 positively regulates salinity tolerance by directly activating OsDREB1B expression in rice.
  • Our data of yeast one-hybrid assay and dual-luciferase assay supported that OsWRKY28 could directly bind to the promoter of OsDREB1B to enhance salinity tolerance in rice.
  • Together, OsWRKY28 confers salinity tolerance through directly targeting OsDREB1B promoter and further activating its transcription in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os09g35010.1
    GCTCAGCTCAAGCTCGCCGGCGAAGTGAACCAGAGAGAGTCATCCATGGAGGTGGAGGAGGCGGCGTACAGGACGGTGTGGTCGGAGCCGCCGAAGAGGCCGGCGGGAAGGACCAAGTTCAGGGAGACGAGGCACCCGGTGTACCGCGGCGTGCGGCGGCGCGGGGGGCGGCCGGGCGCGGCGGGGAGGTGGGTGTGCGAGGTGCGGGTGCCCGGGGCGCGCGGCTCCAGGCTGTGGCTCGGCACGTTCGCCACCGCCGAGGCGGCGGCGCGCGCGCACGACGCCGCCGCGCTGGCGCTCCGCGGCAGGGCCGCCTGCCTCAACTTCGCCGACTCCGCGTGGCGGATGCCGCCCGTCCCCGCGTCCGCCGCGCTCGCCGGCGCGAGGGGGGTCAGGGACGCCGTCGCCGTGGCCGTCGAGGCGTTCCAGCGCCAGTCGGCCGCGCCGTCGTCTCCGGCGGAGACCTTCGCCAACGATGGCGACGAAGAAGAAGACAACAAGGACGTGTTGCCGGTGGCGGCGGCGGAGGTGTTCGACGCGGGGGCGTTCGAGCTCGACGACGGGTTCAGGTTCGGCGGGATGGACGCCGGGTCGTACTACGCGAGCTTGGCGCAGGGGCTGCTCGTCGAGCCGCCGGCCGCCGGAGCGTGGTGGGAGGACGGCGAGCTCGCCGGCTCCGACATGCCGCTCTGGAGCTACTAATCAAAATCTCGCACTGAAAAGTGTGGACAAATTTTGATTCTCCAGAAATTGGGGGAAAAAAGAGAACAGAGTATTGGTGAATTTAGAACAGAGTAGGCAATGAGACTGAGGATGAATGGCAATTTTTGTAATTTTGGAATGTGCCAGATTTCTCCCTCCTTTTGTGATTCCATCTGATTTTGAATGTGCAGTCAATGAATTCCTGTAAATTTACTTCTCCTCTCCAAAGAGCTATTTTGGTCAAGTTCTTCTCTGATTTTATGGATTTTGGATTTACGTT
CDS Sequence
  • >LOC_Os09g35010.1
    ATGGAGGTGGAGGAGGCGGCGTACAGGACGGTGTGGTCGGAGCCGCCGAAGAGGCCGGCGGGAAGGACCAAGTTCAGGGAGACGAGGCACCCGGTGTACCGCGGCGTGCGGCGGCGCGGGGGGCGGCCGGGCGCGGCGGGGAGGTGGGTGTGCGAGGTGCGGGTGCCCGGGGCGCGCGGCTCCAGGCTGTGGCTCGGCACGTTCGCCACCGCCGAGGCGGCGGCGCGCGCGCACGACGCCGCCGCGCTGGCGCTCCGCGGCAGGGCCGCCTGCCTCAACTTCGCCGACTCCGCGTGGCGGATGCCGCCCGTCCCCGCGTCCGCCGCGCTCGCCGGCGCGAGGGGGGTCAGGGACGCCGTCGCCGTGGCCGTCGAGGCGTTCCAGCGCCAGTCGGCCGCGCCGTCGTCTCCGGCGGAGACCTTCGCCAACGATGGCGACGAAGAAGAAGACAACAAGGACGTGTTGCCGGTGGCGGCGGCGGAGGTGTTCGACGCGGGGGCGTTCGAGCTCGACGACGGGTTCAGGTTCGGCGGGATGGACGCCGGGTCGTACTACGCGAGCTTGGCGCAGGGGCTGCTCGTCGAGCCGCCGGCCGCCGGAGCGTGGTGGGAGGACGGCGAGCTCGCCGGCTCCGACATGCCGCTCTGGAGCTACTAA
Protein Sequence
  • >LOC_Os09g35010.1
    MEVEEAAYRTVWSEPPKRPAGRTKFRETRHPVYRGVRRRGGRPGAAGRWVCEVRVPGARGSRLWLGTFATAEAAARAHDAAALALRGRAACLNFADSAWRMPPVPASAALAGARGVRDAVAVAVEAFQRQSAAPSSPAETFANDGDEEEDNKDVLPVAAAEVFDAGAFELDDGFRFGGMDAGSYYASLAQGLLVEPPAAGAWWEDGELAGSDMPLWSY*