Information report for OsDREB1B
Gene Details
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Functional Descriptions
- The OsDREB1B gene was differentially regulated at the transcriptional level by osmotic stress, oxidative stress, salicylic acid, ABA, and cold.
- Histochemical analysis of GUS expression in T(2) transgenic Arabidopsis plants indicated that OsDREB1B shows stress-specific induction pattern in response to a variety of stresses like mannitol, NaCl, PEG, methyl viologen, cold, ABA, and salicylic acid.
- Seven zinc-finger transcription factors are novel regulators of the stress responsive gene OsDREB1B.
- Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high-salt stresses.
- However, here it is shown that OsDREB1B is not only induced by low temperatures, but also by drought and mechanical stress.
- The data obtained provide strong in vivo evidence that OsDREB1B is involved in both abiotic and biotic stress responses, and confers broad-spectrum stress tolerance to transgenic plants.
- Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L.
- Results indicated that the OsDREB1BI gene was over-expressed in transgenic plants under cold and high-temperature, meanwhile, those transgenic plants also revealed freezing and heat tolerance.
- Overexpression of OsDREB1B in tobacco also improved the oxidative and freezing stress tolerance of transgenic plants.
- Under salt stress treatment, the expression levels of OsbZIP05, OsHKT1;1 and OsDREB1B were significantly lower yet the level of OsHKT2;1 was significantly higher in oswrky28 mutants than those in wide type plants.
- OsWRKY28 confers salinity tolerance by directly binding to OsDREB1B promoter and increasing its transcriptional activity, and negatively regulates abscisic acid mediated seedling establishment in rice.
- OsWRKY28 positively regulates salinity tolerance by directly activating OsDREB1B expression in rice.
- Our data of yeast one-hybrid assay and dual-luciferase assay supported that OsWRKY28 could directly bind to the promoter of OsDREB1B to enhance salinity tolerance in rice.
- Together, OsWRKY28 confers salinity tolerance through directly targeting OsDREB1B promoter and further activating its transcription in rice.
Functional Keywords
- salicylic-acid , transcription-factor , salt , temperature , drought , biotic-stress , salt-stress , oxidative , stress , seedling , salinity , tolerance , abscisic-acid
Literature and News
- Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L. . DOI: 10.1007/s11032-006-9065-7
- Seven zinc-finger transcription factors are novel regulators of the stress responsive gene OsDREB1B . DOI: 10.1093/jxb/ers035 ; PMID: 22412187
- OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression . DOI: 10.1046/j.1365-313x.2003.01661.x ; PMID: 12609047
- Colinearity and similar expression pattern of rice DREB1s reveal their functional conservation in the cold-responsive pathway . DOI: 10.1371/journal.pone.0047275 ; PMID: 23077584
- Rice DREB1B promoter shows distinct stress-specific responses, and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance . DOI: 10.1007/s11103-008-9391-8 ; PMID: 18754079
- OsWRKY28 positively regulates salinity tolerance by directly activating OsDREB1B expression in rice . DOI: 10.1007/s00299-022-02950-2 ; PMID: 36350394
Gene Resources
- UniProt: Q3T5N4
- EMBL: AF300972, AP008215, AP014965
- AlphaFoldDB: Q3T5N4
- EnsemblPlants: Os09t0522000-01
- Gramene: Os09t0522000-01
- KEGG: dosa:Os09g0522000
- InterPro: IPR001471, IPR016177, IPR036955
- PANTHER: PTHR31839, PTHR31839:SF57
- SUPFAM: SSF54171
- PROSITE: PS51032
- Gene3D: 3.30.730.10
- OrthoDB: Q3T5N4
- SWISS-MODEL: Q3T5N4
Sequences
cDNA Sequence
- >LOC_Os09g35010.1
GCTCAGCTCAAGCTCGCCGGCGAAGTGAACCAGAGAGAGTCATCCATGGAGGTGGAGGAGGCGGCGTACAGGACGGTGTGGTCGGAGCCGCCGAAGAGGCCGGCGGGAAGGACCAAGTTCAGGGAGACGAGGCACCCGGTGTACCGCGGCGTGCGGCGGCGCGGGGGGCGGCCGGGCGCGGCGGGGAGGTGGGTGTGCGAGGTGCGGGTGCCCGGGGCGCGCGGCTCCAGGCTGTGGCTCGGCACGTTCGCCACCGCCGAGGCGGCGGCGCGCGCGCACGACGCCGCCGCGCTGGCGCTCCGCGGCAGGGCCGCCTGCCTCAACTTCGCCGACTCCGCGTGGCGGATGCCGCCCGTCCCCGCGTCCGCCGCGCTCGCCGGCGCGAGGGGGGTCAGGGACGCCGTCGCCGTGGCCGTCGAGGCGTTCCAGCGCCAGTCGGCCGCGCCGTCGTCTCCGGCGGAGACCTTCGCCAACGATGGCGACGAAGAAGAAGACAACAAGGACGTGTTGCCGGTGGCGGCGGCGGAGGTGTTCGACGCGGGGGCGTTCGAGCTCGACGACGGGTTCAGGTTCGGCGGGATGGACGCCGGGTCGTACTACGCGAGCTTGGCGCAGGGGCTGCTCGTCGAGCCGCCGGCCGCCGGAGCGTGGTGGGAGGACGGCGAGCTCGCCGGCTCCGACATGCCGCTCTGGAGCTACTAATCAAAATCTCGCACTGAAAAGTGTGGACAAATTTTGATTCTCCAGAAATTGGGGGAAAAAAGAGAACAGAGTATTGGTGAATTTAGAACAGAGTAGGCAATGAGACTGAGGATGAATGGCAATTTTTGTAATTTTGGAATGTGCCAGATTTCTCCCTCCTTTTGTGATTCCATCTGATTTTGAATGTGCAGTCAATGAATTCCTGTAAATTTACTTCTCCTCTCCAAAGAGCTATTTTGGTCAAGTTCTTCTCTGATTTTATGGATTTTGGATTTACGTT
CDS Sequence
- >LOC_Os09g35010.1
ATGGAGGTGGAGGAGGCGGCGTACAGGACGGTGTGGTCGGAGCCGCCGAAGAGGCCGGCGGGAAGGACCAAGTTCAGGGAGACGAGGCACCCGGTGTACCGCGGCGTGCGGCGGCGCGGGGGGCGGCCGGGCGCGGCGGGGAGGTGGGTGTGCGAGGTGCGGGTGCCCGGGGCGCGCGGCTCCAGGCTGTGGCTCGGCACGTTCGCCACCGCCGAGGCGGCGGCGCGCGCGCACGACGCCGCCGCGCTGGCGCTCCGCGGCAGGGCCGCCTGCCTCAACTTCGCCGACTCCGCGTGGCGGATGCCGCCCGTCCCCGCGTCCGCCGCGCTCGCCGGCGCGAGGGGGGTCAGGGACGCCGTCGCCGTGGCCGTCGAGGCGTTCCAGCGCCAGTCGGCCGCGCCGTCGTCTCCGGCGGAGACCTTCGCCAACGATGGCGACGAAGAAGAAGACAACAAGGACGTGTTGCCGGTGGCGGCGGCGGAGGTGTTCGACGCGGGGGCGTTCGAGCTCGACGACGGGTTCAGGTTCGGCGGGATGGACGCCGGGTCGTACTACGCGAGCTTGGCGCAGGGGCTGCTCGTCGAGCCGCCGGCCGCCGGAGCGTGGTGGGAGGACGGCGAGCTCGCCGGCTCCGACATGCCGCTCTGGAGCTACTAA
Protein Sequence
- >LOC_Os09g35010.1
MEVEEAAYRTVWSEPPKRPAGRTKFRETRHPVYRGVRRRGGRPGAAGRWVCEVRVPGARGSRLWLGTFATAEAAARAHDAAALALRGRAACLNFADSAWRMPPVPASAALAGARGVRDAVAVAVEAFQRQSAAPSSPAETFANDGDEEEDNKDVLPVAAAEVFDAGAFELDDGFRFGGMDAGSYYASLAQGLLVEPPAAGAWWEDGELAGSDMPLWSY*