Information report for OsGEN-L;OsGEN1
Gene Details
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Functional Descriptions
- OsGEN-L-RNAi plants lacked mature pollen, resulting from a defect in early microspore development.
- Our results suggest that OsGEN-L plays an essential role in DNA metabolism required for early microspore development in rice.
- RNAi-mediated silencing of OsGEN-L (OsGEN-Like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice.
- Most of the OsGEN-L-RNAi plants displayed low fertility, and some of them were male-sterile.
- OsGEN-L has a 5’-flap endonuclease activity and plays an essential role in rice microspore development.
- A OsGEN-L-green fluorescent protein (GFP) fusion protein was localized in the nucleus, and the OsGEN-L promoter was specifically active in the anthers.
- In contrast, depletion of OsSEND1 had no effects on plant development and did not enhance OsGEN1 defects.
- We first investigated the role of OsGEN1 during meiosis and found a reduction in chiasma frequency by ~6% in OsGEN1 mutants, compared to wild type, suggesting a possible involvement of OsGEN1 in the formation of crossovers.
- Post-meiosis, OsGEN1 foci were detected in wild-type microspore nuclei, but not in the OsGEN1 mutant concomitant with an increase in double strand breaks.
Functional Keywords
- microspore , sterile , fertility , anther , pollen , sterility , development , meiosis , plant-development
Literature and News
- The OsGEN-L protein from Oryza sativa possesses Holliday junction resolvase activity as well as 5’-flap endonuclease activity . DOI: 10.1093/jb/mvr145 ; PMID: 22247560
- RNAi-mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice . DOI: 10.1093/pcp/pci090 ; PMID: 15792960
- Resolvase OsGEN1 Mediates DNA Repair by Homologous Recombination . DOI: 10.1104/pp.16.01726 ; PMID: 28049740
Gene Resources
- UniProt: Q64MA3
- EMBL: AB158320, AB194139, AK063534
- AlphaFoldDB: Q64MA3
- EnsemblPlants: Os09t0521900-01
- Gramene: Os09t0521900-01
- Orthologous matrix: RNLYFRT
- InterPro: IPR006084, IPR006085, IPR006086
- PANTHER: PTHR11081, PTHR11081:SF59
- SUPFAM: SSF47807, SSF88723
- Gene3D: 1.10.150.20, 3.40.50.1010
- OrthoDB: Q64MA3
- SWISS-MODEL: Q64MA3
- Conserved Domain Database: cd09869
- eggNOG: KOG2519
Sequences
cDNA Sequence
- >LOC_Os09g35000.1
ACCTGCAGCGACCCAACCCGACCCAACCCAACTCGCGCGAGCCCTGCGGTTTCGCCGGCGAAACGAGGCCGACCTAAACCCTCCGCCGCCGCCGCCGCCGTCGCCGGGGAAGAAGAGAGATGGGGGTGGGGGGAAGCTTCTGGGACCTGCTCAAGCCGTACGCGCGGCACGAGGGCGCGGGGTACCTCCGCGGCCGCCGCGTCGCCGTCGACCTCTCCTTCTGGGTCGTCTCCCACAGCGCCGCCATCCGGGCGCGCTCGCCGCACGCGCGCCTCCCCCATCTCCGCACCCTCTTCTTCCGCACCCTCTCCCTCTTCTCCAAGATGGGTGCTTTTCCTGTGTTCGTGGTGGACGGCCAGCCGTCGCCGCTCAAGTCGCAGGTGAGGGCCGCGCGATTCTTCAGAGGATCTGGAATGGACCTCGCTGCGCTGCCGAGCACGGAGGCGGAGGCCAGTGCGGATGCGCTGGTTCAACCGAGGAATGCCAAGTTCACGCGATACGTGGAGGATTGCGTGGAACTGCTTGAATATCTTGGAATGCCTGTGCTGCGGGCAAAAGGTGAAGGTGAAGCTCTTTGTGCTCAGTTGAATAATCAAGGCCATGTAGATGCTTGCATCACTTCAGACAGTGACGCTTTCCTCTTTGGGGCTAAGACTGTCATAAAGGTTCTCCGATCGAATTGTAAGGAACCTTTTGAGTGCTACAACATGGCAGACATTGAGTCTGGTCTTGGACTAAAGAGGAAACAAATGGTAGCAATGGCACTTCTTGTTGGCAGTGACCATGACTTGCATGGTGTGCCTGGTTTTGGTCCTGAGACTGCACTTCGGTTTGTGCAGTTATTTGATGAAGATAATGTTCTAGCTAAATTATATGAAATTGGTAAAGGGGTCTATCCATTTATAGGAGTTAGTGCGCCCAATATTGATGATCTCCCATCACCCTCCACAAAGAGTCTACCGCGTGCGCGATCACCGCACTGCTCACATTGTGGTCACCCTGGCAACAAGAAAAATCATATCAAGGATGGGTGCAACTTTTGCTTGGTTGATTCATTAGAGAATTGTGTGGAGAAGCCAGCTGGATTCATATGTGAATGTCCTAGTTGTGACAAGGCACGTGATCTGAAGGTGCAGAGAAGAAATGAAAACTGGCAAATCAAGGTCTGTAAAAGGATAGCTGCTGAGACTAACTTTCCAAATGAGGAGATCATTAACTTGTACTTGAATGATGACAATTTGGATAATGAAAATGGTGTTCCATTGCTTACATGGAATAAACCTGATATGGAGATTTTGGTTGACTTCTTATCTTTCAAGCAGAACTGGGAGCCAGCGTATATTCGTCAGCGGATGCTTCCTATGCTATCAACCATATATTTACGCGAAATGGCTTCATCTCAATCTAAATCATTTCTTCTTTATGATCAATATAAATTTCATTCAATCCAACGGATTAAGATAAGATATGGTCACCCATATTACTTGGTCAAGTGGAAGAGAGTCACTCGTAGTATGATATCCAATGATCCACCTAGCAAGCAGACAGAATTGGAGGGGAAGAATGATAAGGTGGAGGTATTGGATGGAGATGATGAGGTAGTAGATGAAGAGGAGGAGGAGCCTACAATGATTTCCGAAACTACTGAACTGCTAGATGAGCCAGATGTTCCACAAGTACTGGATGATGATAAAGATTGCTTTTTATTAACTGATGAAGACATTGAGCTAGTGAATGCTGCATTTCCTGATGAAGCACAAAGATTTCAGGAAGAACAGAGGCTGAAAGAAGCAAAGTCAATAGCACGGAAATCCAAGCTCAACGTGGCAGGTTTCGAAACACCAAAAGGTCCAAGGCCTAGTGGAGTTCAGCTTAGCATCAAGGAGTTCTACCGCTCCAAGAAAGGGCTGAGTGGCGACTCAGGGAAGGACGGCTCAAGAAAATCCTCCGATGTGGACCTCAGCAAGAACTTGCCCAAGTCTGTCCGACGACGCCTCCTCTTCGACTGACTGACAATTGGTCTCCCTTCTGTCTTCCCAGTAGTCCTGCTGCTACTGTTGTACATGTTCCAGTAACATTTCTTTCGGCCAATGGATGTACATTGCGTGGCCCGGTTTTTGTACAGGGAACATGCATGTCTTGTAGCTGATGAGAAACAGTGTTTTGGGGGTTGAATCTTGTATCTGCTGCAACCATGTATATTTCATGCCATATCGCATCACTGCTTGCGTAGCATGGAAGAATTGGACCTGAAATGAGCTGTACCAGCTACAGTGCTTCCTTTGGACCAACAGTGTCAGCTCTACACAAGGGTCAATTCTGTCTACGGCGATGAAAAGTGAAGAGTTCATCACCAAAAAGGTGAAAAATGTGGACAGTTTTTTGCAACGAAGATTTATGCCCTTCCTCACAAGGAGTTGACCTTGAACGTATATACAATCTTATACTTGTGTGTGTACTTAATATCTGATACTGTTGGACCCAGT
CDS Sequence
- >LOC_Os09g35000.1
ATGGGGGTGGGGGGAAGCTTCTGGGACCTGCTCAAGCCGTACGCGCGGCACGAGGGCGCGGGGTACCTCCGCGGCCGCCGCGTCGCCGTCGACCTCTCCTTCTGGGTCGTCTCCCACAGCGCCGCCATCCGGGCGCGCTCGCCGCACGCGCGCCTCCCCCATCTCCGCACCCTCTTCTTCCGCACCCTCTCCCTCTTCTCCAAGATGGGTGCTTTTCCTGTGTTCGTGGTGGACGGCCAGCCGTCGCCGCTCAAGTCGCAGGTGAGGGCCGCGCGATTCTTCAGAGGATCTGGAATGGACCTCGCTGCGCTGCCGAGCACGGAGGCGGAGGCCAGTGCGGATGCGCTGGTTCAACCGAGGAATGCCAAGTTCACGCGATACGTGGAGGATTGCGTGGAACTGCTTGAATATCTTGGAATGCCTGTGCTGCGGGCAAAAGGTGAAGGTGAAGCTCTTTGTGCTCAGTTGAATAATCAAGGCCATGTAGATGCTTGCATCACTTCAGACAGTGACGCTTTCCTCTTTGGGGCTAAGACTGTCATAAAGGTTCTCCGATCGAATTGTAAGGAACCTTTTGAGTGCTACAACATGGCAGACATTGAGTCTGGTCTTGGACTAAAGAGGAAACAAATGGTAGCAATGGCACTTCTTGTTGGCAGTGACCATGACTTGCATGGTGTGCCTGGTTTTGGTCCTGAGACTGCACTTCGGTTTGTGCAGTTATTTGATGAAGATAATGTTCTAGCTAAATTATATGAAATTGGTAAAGGGGTCTATCCATTTATAGGAGTTAGTGCGCCCAATATTGATGATCTCCCATCACCCTCCACAAAGAGTCTACCGCGTGCGCGATCACCGCACTGCTCACATTGTGGTCACCCTGGCAACAAGAAAAATCATATCAAGGATGGGTGCAACTTTTGCTTGGTTGATTCATTAGAGAATTGTGTGGAGAAGCCAGCTGGATTCATATGTGAATGTCCTAGTTGTGACAAGGCACGTGATCTGAAGGTGCAGAGAAGAAATGAAAACTGGCAAATCAAGGTCTGTAAAAGGATAGCTGCTGAGACTAACTTTCCAAATGAGGAGATCATTAACTTGTACTTGAATGATGACAATTTGGATAATGAAAATGGTGTTCCATTGCTTACATGGAATAAACCTGATATGGAGATTTTGGTTGACTTCTTATCTTTCAAGCAGAACTGGGAGCCAGCGTATATTCGTCAGCGGATGCTTCCTATGCTATCAACCATATATTTACGCGAAATGGCTTCATCTCAATCTAAATCATTTCTTCTTTATGATCAATATAAATTTCATTCAATCCAACGGATTAAGATAAGATATGGTCACCCATATTACTTGGTCAAGTGGAAGAGAGTCACTCGTAGTATGATATCCAATGATCCACCTAGCAAGCAGACAGAATTGGAGGGGAAGAATGATAAGGTGGAGGTATTGGATGGAGATGATGAGGTAGTAGATGAAGAGGAGGAGGAGCCTACAATGATTTCCGAAACTACTGAACTGCTAGATGAGCCAGATGTTCCACAAGTACTGGATGATGATAAAGATTGCTTTTTATTAACTGATGAAGACATTGAGCTAGTGAATGCTGCATTTCCTGATGAAGCACAAAGATTTCAGGAAGAACAGAGGCTGAAAGAAGCAAAGTCAATAGCACGGAAATCCAAGCTCAACGTGGCAGGTTTCGAAACACCAAAAGGTCCAAGGCCTAGTGGAGTTCAGCTTAGCATCAAGGAGTTCTACCGCTCCAAGAAAGGGCTGAGTGGCGACTCAGGGAAGGACGGCTCAAGAAAATCCTCCGATGTGGACCTCAGCAAGAACTTGCCCAAGTCTGTCCGACGACGCCTCCTCTTCGACTGA
Protein Sequence
- >LOC_Os09g35000.1
MGVGGSFWDLLKPYARHEGAGYLRGRRVAVDLSFWVVSHSAAIRARSPHARLPHLRTLFFRTLSLFSKMGAFPVFVVDGQPSPLKSQVRAARFFRGSGMDLAALPSTEAEASADALVQPRNAKFTRYVEDCVELLEYLGMPVLRAKGEGEALCAQLNNQGHVDACITSDSDAFLFGAKTVIKVLRSNCKEPFECYNMADIESGLGLKRKQMVAMALLVGSDHDLHGVPGFGPETALRFVQLFDEDNVLAKLYEIGKGVYPFIGVSAPNIDDLPSPSTKSLPRARSPHCSHCGHPGNKKNHIKDGCNFCLVDSLENCVEKPAGFICECPSCDKARDLKVQRRNENWQIKVCKRIAAETNFPNEEIINLYLNDDNLDNENGVPLLTWNKPDMEILVDFLSFKQNWEPAYIRQRMLPMLSTIYLREMASSQSKSFLLYDQYKFHSIQRIKIRYGHPYYLVKWKRVTRSMISNDPPSKQTELEGKNDKVEVLDGDDEVVDEEEEEPTMISETTELLDEPDVPQVLDDDKDCFLLTDEDIELVNAAFPDEAQRFQEEQRLKEAKSIARKSKLNVAGFETPKGPRPSGVQLSIKEFYRSKKGLSGDSGKDGSRKSSDVDLSKNLPKSVRRRLLFD*