Gene Details:

Functional Descriptions:

  • OsGEN-L-RNAi plants lacked mature pollen, resulting from a defect in early microspore development.
  • Our results suggest that OsGEN-L plays an essential role in DNA metabolism required for early microspore development in rice.
  • RNAi-mediated silencing of OsGEN-L (OsGEN-Like), a new member of the RAD2/XPG nuclease family, causes male sterility by defect of microspore development in rice.
  • Most of the OsGEN-L-RNAi plants displayed low fertility, and some of them were male-sterile.
  • OsGEN-L has a 5’-flap endonuclease activity and plays an essential role in rice microspore development.
  • A OsGEN-L-green fluorescent protein (GFP) fusion protein was localized in the nucleus, and the OsGEN-L promoter was specifically active in the anthers.
  • In contrast, depletion of OsSEND1 had no effects on plant development and did not enhance OsGEN1 defects.
  • We first investigated the role of OsGEN1 during meiosis and found a reduction in chiasma frequency by ~6% in OsGEN1 mutants, compared to wild type, suggesting a possible involvement of OsGEN1 in the formation of crossovers.
  • Post-meiosis, OsGEN1 foci were detected in wild-type microspore nuclei, but not in the OsGEN1 mutant concomitant with an increase in double strand breaks.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os09g35000.1
    ACCTGCAGCGACCCAACCCGACCCAACCCAACTCGCGCGAGCCCTGCGGTTTCGCCGGCGAAACGAGGCCGACCTAAACCCTCCGCCGCCGCCGCCGCCGTCGCCGGGGAAGAAGAGAGATGGGGGTGGGGGGAAGCTTCTGGGACCTGCTCAAGCCGTACGCGCGGCACGAGGGCGCGGGGTACCTCCGCGGCCGCCGCGTCGCCGTCGACCTCTCCTTCTGGGTCGTCTCCCACAGCGCCGCCATCCGGGCGCGCTCGCCGCACGCGCGCCTCCCCCATCTCCGCACCCTCTTCTTCCGCACCCTCTCCCTCTTCTCCAAGATGGGTGCTTTTCCTGTGTTCGTGGTGGACGGCCAGCCGTCGCCGCTCAAGTCGCAGGTGAGGGCCGCGCGATTCTTCAGAGGATCTGGAATGGACCTCGCTGCGCTGCCGAGCACGGAGGCGGAGGCCAGTGCGGATGCGCTGGTTCAACCGAGGAATGCCAAGTTCACGCGATACGTGGAGGATTGCGTGGAACTGCTTGAATATCTTGGAATGCCTGTGCTGCGGGCAAAAGGTGAAGGTGAAGCTCTTTGTGCTCAGTTGAATAATCAAGGCCATGTAGATGCTTGCATCACTTCAGACAGTGACGCTTTCCTCTTTGGGGCTAAGACTGTCATAAAGGTTCTCCGATCGAATTGTAAGGAACCTTTTGAGTGCTACAACATGGCAGACATTGAGTCTGGTCTTGGACTAAAGAGGAAACAAATGGTAGCAATGGCACTTCTTGTTGGCAGTGACCATGACTTGCATGGTGTGCCTGGTTTTGGTCCTGAGACTGCACTTCGGTTTGTGCAGTTATTTGATGAAGATAATGTTCTAGCTAAATTATATGAAATTGGTAAAGGGGTCTATCCATTTATAGGAGTTAGTGCGCCCAATATTGATGATCTCCCATCACCCTCCACAAAGAGTCTACCGCGTGCGCGATCACCGCACTGCTCACATTGTGGTCACCCTGGCAACAAGAAAAATCATATCAAGGATGGGTGCAACTTTTGCTTGGTTGATTCATTAGAGAATTGTGTGGAGAAGCCAGCTGGATTCATATGTGAATGTCCTAGTTGTGACAAGGCACGTGATCTGAAGGTGCAGAGAAGAAATGAAAACTGGCAAATCAAGGTCTGTAAAAGGATAGCTGCTGAGACTAACTTTCCAAATGAGGAGATCATTAACTTGTACTTGAATGATGACAATTTGGATAATGAAAATGGTGTTCCATTGCTTACATGGAATAAACCTGATATGGAGATTTTGGTTGACTTCTTATCTTTCAAGCAGAACTGGGAGCCAGCGTATATTCGTCAGCGGATGCTTCCTATGCTATCAACCATATATTTACGCGAAATGGCTTCATCTCAATCTAAATCATTTCTTCTTTATGATCAATATAAATTTCATTCAATCCAACGGATTAAGATAAGATATGGTCACCCATATTACTTGGTCAAGTGGAAGAGAGTCACTCGTAGTATGATATCCAATGATCCACCTAGCAAGCAGACAGAATTGGAGGGGAAGAATGATAAGGTGGAGGTATTGGATGGAGATGATGAGGTAGTAGATGAAGAGGAGGAGGAGCCTACAATGATTTCCGAAACTACTGAACTGCTAGATGAGCCAGATGTTCCACAAGTACTGGATGATGATAAAGATTGCTTTTTATTAACTGATGAAGACATTGAGCTAGTGAATGCTGCATTTCCTGATGAAGCACAAAGATTTCAGGAAGAACAGAGGCTGAAAGAAGCAAAGTCAATAGCACGGAAATCCAAGCTCAACGTGGCAGGTTTCGAAACACCAAAAGGTCCAAGGCCTAGTGGAGTTCAGCTTAGCATCAAGGAGTTCTACCGCTCCAAGAAAGGGCTGAGTGGCGACTCAGGGAAGGACGGCTCAAGAAAATCCTCCGATGTGGACCTCAGCAAGAACTTGCCCAAGTCTGTCCGACGACGCCTCCTCTTCGACTGACTGACAATTGGTCTCCCTTCTGTCTTCCCAGTAGTCCTGCTGCTACTGTTGTACATGTTCCAGTAACATTTCTTTCGGCCAATGGATGTACATTGCGTGGCCCGGTTTTTGTACAGGGAACATGCATGTCTTGTAGCTGATGAGAAACAGTGTTTTGGGGGTTGAATCTTGTATCTGCTGCAACCATGTATATTTCATGCCATATCGCATCACTGCTTGCGTAGCATGGAAGAATTGGACCTGAAATGAGCTGTACCAGCTACAGTGCTTCCTTTGGACCAACAGTGTCAGCTCTACACAAGGGTCAATTCTGTCTACGGCGATGAAAAGTGAAGAGTTCATCACCAAAAAGGTGAAAAATGTGGACAGTTTTTTGCAACGAAGATTTATGCCCTTCCTCACAAGGAGTTGACCTTGAACGTATATACAATCTTATACTTGTGTGTGTACTTAATATCTGATACTGTTGGACCCAGT
CDS Sequence
  • >LOC_Os09g35000.1
    ATGGGGGTGGGGGGAAGCTTCTGGGACCTGCTCAAGCCGTACGCGCGGCACGAGGGCGCGGGGTACCTCCGCGGCCGCCGCGTCGCCGTCGACCTCTCCTTCTGGGTCGTCTCCCACAGCGCCGCCATCCGGGCGCGCTCGCCGCACGCGCGCCTCCCCCATCTCCGCACCCTCTTCTTCCGCACCCTCTCCCTCTTCTCCAAGATGGGTGCTTTTCCTGTGTTCGTGGTGGACGGCCAGCCGTCGCCGCTCAAGTCGCAGGTGAGGGCCGCGCGATTCTTCAGAGGATCTGGAATGGACCTCGCTGCGCTGCCGAGCACGGAGGCGGAGGCCAGTGCGGATGCGCTGGTTCAACCGAGGAATGCCAAGTTCACGCGATACGTGGAGGATTGCGTGGAACTGCTTGAATATCTTGGAATGCCTGTGCTGCGGGCAAAAGGTGAAGGTGAAGCTCTTTGTGCTCAGTTGAATAATCAAGGCCATGTAGATGCTTGCATCACTTCAGACAGTGACGCTTTCCTCTTTGGGGCTAAGACTGTCATAAAGGTTCTCCGATCGAATTGTAAGGAACCTTTTGAGTGCTACAACATGGCAGACATTGAGTCTGGTCTTGGACTAAAGAGGAAACAAATGGTAGCAATGGCACTTCTTGTTGGCAGTGACCATGACTTGCATGGTGTGCCTGGTTTTGGTCCTGAGACTGCACTTCGGTTTGTGCAGTTATTTGATGAAGATAATGTTCTAGCTAAATTATATGAAATTGGTAAAGGGGTCTATCCATTTATAGGAGTTAGTGCGCCCAATATTGATGATCTCCCATCACCCTCCACAAAGAGTCTACCGCGTGCGCGATCACCGCACTGCTCACATTGTGGTCACCCTGGCAACAAGAAAAATCATATCAAGGATGGGTGCAACTTTTGCTTGGTTGATTCATTAGAGAATTGTGTGGAGAAGCCAGCTGGATTCATATGTGAATGTCCTAGTTGTGACAAGGCACGTGATCTGAAGGTGCAGAGAAGAAATGAAAACTGGCAAATCAAGGTCTGTAAAAGGATAGCTGCTGAGACTAACTTTCCAAATGAGGAGATCATTAACTTGTACTTGAATGATGACAATTTGGATAATGAAAATGGTGTTCCATTGCTTACATGGAATAAACCTGATATGGAGATTTTGGTTGACTTCTTATCTTTCAAGCAGAACTGGGAGCCAGCGTATATTCGTCAGCGGATGCTTCCTATGCTATCAACCATATATTTACGCGAAATGGCTTCATCTCAATCTAAATCATTTCTTCTTTATGATCAATATAAATTTCATTCAATCCAACGGATTAAGATAAGATATGGTCACCCATATTACTTGGTCAAGTGGAAGAGAGTCACTCGTAGTATGATATCCAATGATCCACCTAGCAAGCAGACAGAATTGGAGGGGAAGAATGATAAGGTGGAGGTATTGGATGGAGATGATGAGGTAGTAGATGAAGAGGAGGAGGAGCCTACAATGATTTCCGAAACTACTGAACTGCTAGATGAGCCAGATGTTCCACAAGTACTGGATGATGATAAAGATTGCTTTTTATTAACTGATGAAGACATTGAGCTAGTGAATGCTGCATTTCCTGATGAAGCACAAAGATTTCAGGAAGAACAGAGGCTGAAAGAAGCAAAGTCAATAGCACGGAAATCCAAGCTCAACGTGGCAGGTTTCGAAACACCAAAAGGTCCAAGGCCTAGTGGAGTTCAGCTTAGCATCAAGGAGTTCTACCGCTCCAAGAAAGGGCTGAGTGGCGACTCAGGGAAGGACGGCTCAAGAAAATCCTCCGATGTGGACCTCAGCAAGAACTTGCCCAAGTCTGTCCGACGACGCCTCCTCTTCGACTGA
Protein Sequence
  • >LOC_Os09g35000.1
    MGVGGSFWDLLKPYARHEGAGYLRGRRVAVDLSFWVVSHSAAIRARSPHARLPHLRTLFFRTLSLFSKMGAFPVFVVDGQPSPLKSQVRAARFFRGSGMDLAALPSTEAEASADALVQPRNAKFTRYVEDCVELLEYLGMPVLRAKGEGEALCAQLNNQGHVDACITSDSDAFLFGAKTVIKVLRSNCKEPFECYNMADIESGLGLKRKQMVAMALLVGSDHDLHGVPGFGPETALRFVQLFDEDNVLAKLYEIGKGVYPFIGVSAPNIDDLPSPSTKSLPRARSPHCSHCGHPGNKKNHIKDGCNFCLVDSLENCVEKPAGFICECPSCDKARDLKVQRRNENWQIKVCKRIAAETNFPNEEIINLYLNDDNLDNENGVPLLTWNKPDMEILVDFLSFKQNWEPAYIRQRMLPMLSTIYLREMASSQSKSFLLYDQYKFHSIQRIKIRYGHPYYLVKWKRVTRSMISNDPPSKQTELEGKNDKVEVLDGDDEVVDEEEEEPTMISETTELLDEPDVPQVLDDDKDCFLLTDEDIELVNAAFPDEAQRFQEEQRLKEAKSIARKSKLNVAGFETPKGPRPSGVQLSIKEFYRSKKGLSGDSGKDGSRKSSDVDLSKNLPKSVRRRLLFD*