Gene Details:

Functional Descriptions:

  • Transgenic rice plants that overexpress transcription factors RF2a and RF2b are tolerant to rice tungro virus replication and disease.
  • RF2a, which stimulates Box II-dependent transcription in a homologous in vitro transcription system, accumulates in nuclei of phloem and certain other cell types in shoots, but is found at only very low levels in roots.
  • Conversely, lines with elevated expression of RF2a or RF2b showed weak or no symptoms of infection after Agrobacterium inoculation of RTBV, whereas control plants showed severe stunting and leaf discoloration.
  • RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease.
  • When a fusion gene comprising the cauliflower mosaic virus 35S promoter and RF2a cDNA was coexpressed with the GUS reporter genes, GUS activity was increased by 2-20-fold.
  • Transcription factor RF2a alters expression of the rice tungro bacilliform virus promoter in transgenic tobacco plants.
  • Rice transcription factor RF2a binds to the BoxII cis element of the promoter of rice tungro bacilliform virus and activates promoter expression.
  • Functional analysis of the activation domain of RF2a, a rice transcription factor.
  • Transgenic antisense plants in which RF2a accumulation was suppressed had normal roots but stunted, twisted leaves with small, disorganized vascular bundles, an enlarged sclerenchyma and large air spaces.
  • Two host transcription factors, RF2a and RF2b, regulate expression of the RTBV promoter and are important for plant development.
  • We also demonstrate a physical interaction between OsTBP2 and RF2a, a rice bZIP transcription factor that bound to the box II cis element of the promoter of Rice tungro bacilliform virus, resulting in enhanced transcription from the viral promoter.
  • We conclude that OsTBP2 undergoes promoter-specific functional interactions with both the basal transcription factor OsTFIIB and the accessory transcription factor RF2a.
  • Rice TATA Binding Protein Interacts Functionally with Transcription Factor IIB and the RF2a bZIP Transcriptional Activator in an Enhanced Plant in Vitro Transcription System.
  • RF2a is a bZIP transcription factor that regulates expression of the promoter of rice tungro bacilliform badnavirus.
  • Functional analysis of RF2a, a rice transcription factor.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os09g34060.1
    GCTTGTTGCATTTTACTGTCCCCATGCAGACAATGCCAAAGGGATAAACAGAGCTAAAATTGGTGAAAATTCACTCGAATCCAAATGTAAACAACCCATTTTTCTCCTGTTCCAATCCCATCCATTCCATTCCATTCCTTCTCCCTGTGCTCGGATTCCTGTTCTTGCAGCTGATCCCTAGATCTTCCTCTTTGCCCGAGAATTTTCCGCCCAATTCCCCACTCCGATTCCTCTTCTTTCGCCCAAATTTTGGCTCAACATTTCGCAGCGCAATCTCGAGCTGAATCTTGGGGGGTAAAGCTTTGCTTCTTGGAATCTTGGTGCGGTTTTTGGGGGAGATTTGCGAGCGTTTGGAGCCGAGAAGATGAACAGGGAGAAATCCCCGATCCCGGGAGACGGCGGCGACGGGTTGCCGCCGCAGGCCACCCGCCGGGCGGGCCCTCCGGCGGCGGCGGCGGCGGCGGAGTACGACATCAGCCGCATGCCGGATTTCCCGACGAGGAACCCCGGCCACAGGCGCGCCCACTCCGAGATCCTGAGCCTCCCCGAGGACCTCGACCTGTGCGCGGCCGGCGGCGGCGACGGGCCGTCGCTGTCGGACGAGAACGACGAGGAGCTCTTCTCCATGTTCCTCGACGTGGAGAAGCTGAACAGCACGTGCGGGGCGTCGTCGGAGGCGGAGGCGGAGTCGTCGTCCGCCGGCGCCGCGGCGGCGGTGGCGGCGGCGGCCGCCGCCGCCGCCCATGGAGCGAGGCCGAAGCACCAGCACAGCCTGTCCATGGATGAGTCGATGTCGATCAAGGCTGAGGAGCTCGTCGGGGCGTCGCCCGGGACGGAGGGGATGTCGTCGGCGGAGGCCAAGAAGGCCGTGTCCGCGGCCAAGCTCGCCGAGCTTGCTCTCGTCGATCCCAAGAGGGCGAAAAGGATTTGGGCTAACAGACAATCTGCGGCAAGATCAAAGGAAAGGAAAATGCGATATATTGCTGAACTTGAGCGCAAGGTGCAAACCCTGCAAACAGAAGCAACAACACTGTCAGCCCAGTTGGCACTGCTACAGAGAGATACCAGTGGGCTAACTACTGAGAATAGTGAACTGAAGCTACGTCTGCAGACCATGGAGCAGCAAGTCCACTTGCAAGATGCTTTGAATGACACCCTGAAGTCTGAGGTTCAGCGGCTTAAGGTTGCAACCGGTCAAATGGCGAATGGTGGAGGGATGATGATGAACTTCGGTGGCATGCCACACCAATTCGGAGGCAACCAGCAGATGTTCCAGAACAACCAGGCCATGCAATCTATGCTGGCAGCACACCAGCTGCAACAGCTCCAGCTTCATCCTCAGGCTCAGCAGCAGCAGGTGCTGCACCCTCAGCATCAGCAGCAGCAGCCATTGCACCCTCTACAAGCGCAGCAGCTCCAGCAGGCGGCACGAGACCTCAAGATGAAATCGCCGATGGGCGGCCAGAGCCAGTGGGGAGATGGCAAGTCAGGAAGCAGCGGCAACTGATGCGTCATTTACAAATTTACAAAAAAATTCCCCAAAGTTTCGGTGTAAGGTTGTGGTTTAACAACCATGGAGATGGTCTGTTTCATTGAGATGATGATGATGTGCAAGAGTAGTTATCATCATCATCATCGGTAGGTTCAGGTTGGCTTCAGCTTCAGTCATACAGTTGGGTGGATGTTCAGTTGAAGAGTTTATCTGTAGCTGGTTCAGTAAGGAAATGGTCGAATAATCAAGAATTCCAGGTTAAAATCTTTCGTTTCTTTGGTAAGGTAGCACATCACCAAGATGTACTGTTGTAGTGTTGTCTACAGTTCCATAAATTCAAGTAGCCAGTATACGCTGTTGTTTAGTTTTTCTCCGGTTTTTTAGAGGTAGAGGCGTTTCTAGTGTTAATCGAGAGGTATACTGTATACCTTCTGATTAACATGTAAATCACTCAACACCTCTACCTCCATAAGGACCGGAGAAAAACTCAATTGTTTAATTAAGATGCCATTCATGACTAGATGTTCTCTGTCAGATGATCATATTGCACTCTGCTCCCTCTGCCTCATTAAAAAAAAATAAACCTAGCACACATGATGTGAGCGTC
CDS Sequence
  • >LOC_Os09g34060.1
    ATGAACAGGGAGAAATCCCCGATCCCGGGAGACGGCGGCGACGGGTTGCCGCCGCAGGCCACCCGCCGGGCGGGCCCTCCGGCGGCGGCGGCGGCGGCGGAGTACGACATCAGCCGCATGCCGGATTTCCCGACGAGGAACCCCGGCCACAGGCGCGCCCACTCCGAGATCCTGAGCCTCCCCGAGGACCTCGACCTGTGCGCGGCCGGCGGCGGCGACGGGCCGTCGCTGTCGGACGAGAACGACGAGGAGCTCTTCTCCATGTTCCTCGACGTGGAGAAGCTGAACAGCACGTGCGGGGCGTCGTCGGAGGCGGAGGCGGAGTCGTCGTCCGCCGGCGCCGCGGCGGCGGTGGCGGCGGCGGCCGCCGCCGCCGCCCATGGAGCGAGGCCGAAGCACCAGCACAGCCTGTCCATGGATGAGTCGATGTCGATCAAGGCTGAGGAGCTCGTCGGGGCGTCGCCCGGGACGGAGGGGATGTCGTCGGCGGAGGCCAAGAAGGCCGTGTCCGCGGCCAAGCTCGCCGAGCTTGCTCTCGTCGATCCCAAGAGGGCGAAAAGGATTTGGGCTAACAGACAATCTGCGGCAAGATCAAAGGAAAGGAAAATGCGATATATTGCTGAACTTGAGCGCAAGGTGCAAACCCTGCAAACAGAAGCAACAACACTGTCAGCCCAGTTGGCACTGCTACAGAGAGATACCAGTGGGCTAACTACTGAGAATAGTGAACTGAAGCTACGTCTGCAGACCATGGAGCAGCAAGTCCACTTGCAAGATGCTTTGAATGACACCCTGAAGTCTGAGGTTCAGCGGCTTAAGGTTGCAACCGGTCAAATGGCGAATGGTGGAGGGATGATGATGAACTTCGGTGGCATGCCACACCAATTCGGAGGCAACCAGCAGATGTTCCAGAACAACCAGGCCATGCAATCTATGCTGGCAGCACACCAGCTGCAACAGCTCCAGCTTCATCCTCAGGCTCAGCAGCAGCAGGTGCTGCACCCTCAGCATCAGCAGCAGCAGCCATTGCACCCTCTACAAGCGCAGCAGCTCCAGCAGGCGGCACGAGACCTCAAGATGAAATCGCCGATGGGCGGCCAGAGCCAGTGGGGAGATGGCAAGTCAGGAAGCAGCGGCAACTGA
Protein Sequence
  • >LOC_Os09g34060.1
    MNREKSPIPGDGGDGLPPQATRRAGPPAAAAAAEYDISRMPDFPTRNPGHRRAHSEILSLPEDLDLCAAGGGDGPSLSDENDEELFSMFLDVEKLNSTCGASSEAEAESSSAGAAAAVAAAAAAAAHGARPKHQHSLSMDESMSIKAEELVGASPGTEGMSSAEAKKAVSAAKLAELALVDPKRAKRIWANRQSAARSKERKMRYIAELERKVQTLQTEATTLSAQLALLQRDTSGLTTENSELKLRLQTMEQQVHLQDALNDTLKSEVQRLKVATGQMANGGGMMMNFGGMPHQFGGNQQMFQNNQAMQSMLAAHQLQQLQLHPQAQQQQVLHPQHQQQQPLHPLQAQQLQQAARDLKMKSPMGGQSQWGDGKSGSSGN*