Gene Details:

• MSU gene ID: LOC_Os09g29404
• RAPdb gene ID: Os09g0469400
• Gene Symbol: ISA3 OsISA3
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Furthermore, chloroplasts in the leaf blade of ISA3 seedlings were large and pleomorphic.
• We investigated whether ISA3 plays a role in amyloplast development and starch metabolism in the developing endosperm.
• ISA3-green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm.
• Both overexpression and loss of function of ISA3 in the endosperm generated pleomorphic amyloplasts and starch granules.
• A transposon-inserted rice mutant of isoamylase3 (ISA3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night.
• Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant.
• These results suggest that ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice.
• Systematic analyses of the transgenic rice indicated that co-overexpression of OsBMY4 and OsISA3 not only promoted rice yield and quality, but also improved seed germination and stress tolerance.

Function-related keywords:

• chloroplast ,  endosperm ,  starch ,  leaf ,  seedling ,  stress ,  seed ,  seed-germination ,  tolerance ,  yield ,  stress-tolerance

Literature:

• Rice debranching enzyme isoamylase3 facilitates starch metabolism and affects plastid morphogenesis .  DOI:  10.1093/pcp/pcr058 ;  PMID:  21551159
• Co-Overexpression of Two Key Source Genes, OsBMY4 and OsISA3, Improves Multiple Key Traits of Rice Seeds .  DOI:  10.1021/acs.jafc.2c06039 ;  PMID:  36537359

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: