Information report for OsABA8ox3
Gene Details
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Functional Descriptions
- Further experiments indicated that the expression of OsABA8ox3, a key gene in ABA catabolism and encoding ABA 8’-hydroxylase in rice, was significantly increased during the first 6 h of imbibition, which was consistent with the decline of ABA content in the imbibed seeds.
- Enzyme activity analysis indicated that OsABA8ox3 RNAi plants had higher superoxide dismutase (SOD) and catalase (CAT) activities and less malondialdehyde (MDA) content than those of wild type when the plants were exposed to dehydration treatment, indicating a better anti-oxidative stress capability and less membrane damage.
- However, OsABA8ox3 RNAi lines showed significant improvement in drought stress tolerance while the overexpression seedlings were hypersensitive to drought stress when compared with wild type in terms of plant survival rates after 10 days of unwatering.
- We therefore conclude that that OsABA8ox3 gene plays an important role in controlling ABA level and drought stress resistance in rice.
- Expression of OsABA8ox3 was promptly induced by rehydration after PEG-mimic dehydration, a tendency opposite to the changes of ABA level.
- LEA genes, were enhanced with higher transcript levels in OsABA8ox3 RNAi transgenic seedlings.
Functional Keywords
- seed , ABA , oxidative-stress , drought , tolerance , stress , drought-stress , stress-tolerance , seedlings , resistance
Literature and News
- Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L . DOI: 10.1007/s00425-013-1853-9 ; PMID: 23397192
- ABA regulates apoplastic sugar transport and is a potential signal for cold-induced pollen sterility in rice . DOI: 10.1093/pcp/pcm100 ; PMID: 17693452
- Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8’-hydroxylase in rice . DOI: 10.1093/pcp/pcm003 ; PMID: 17205969
- Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis . DOI: 10.1093/pcp/pcp022 ; PMID: 19208695
- A key ABA catabolic gene, OsABA8ox3, is involved in drought stress resistance in rice . DOI: 10.1371/journal.pone.0116646 ; PMID: 25647508
- Improving pre-harvest sprouting resistance in rice by editing OsABA8ox using CRISPR/Cas9 . DOI: 10.1007/s00299-022-02917-3 ; PMID: 35976402
- Antagonistic control of seed dormancy in rice by two bHLH transcription factors . DOI: 10.1038/s41588-022-01240-7 ; PMID: 36471073
Gene Resources
- UniProt: Q0J185
- EMBL: AP006057, AP008215, AP014965
- AlphaFoldDB: Q0J185
- EnsemblPlants: Os09t0457100-01
- Gramene: Os09t0457100-01
- KEGG: dosa:Os09g0457100
- Orthologous matrix: TPPFHGK
- InterPro: IPR001128, IPR002401, IPR017972
- PANTHER: PTHR24286, PTHR24286:SF376
- SUPFAM: SSF48264
- PROSITE: PS00086
- Gene3D: 1.10.630.10
- OrthoDB: Q0J185
- SWISS-MODEL: Q0J185
- Conserved Domain Database: cd11043
- eggNOG: KOG0157
Sequences
cDNA Sequence
- >LOC_Os09g28390.1
CCTCGAAGATTTTTCTCCTCCCTCTCTCCCTCTCCTCACAGCTCTCTCCCTCTCTCCTAGCAACAGCATATATAGCGCAAAGACAATAACACACGACTAGCTAGCAAGAAGAAGACAGCTGGTTCAGAACTCTCAAAACAGCTGTGGTGTCCAAGTGATCCTTAATTCTCCAGGGTCATTTGGATGGCAGCCTCCTTCGTCATCGTCATCGTCATCTCCTTCTTCATTTCTCTTGCTTTTATGTGCTATGTCCACTACACGAGCCGGCAGAGGAGGAAACTCCATGGCTACGGCCATGAGAAAGCCGTCAGGCTGCCGCCGGGCTCCATGGGTTGGCCTTACATCGGCGAGACCCTTCAGCTCTACTCCCAAGACCCCAACGTCTTCTTCGCCTCCAAACAGAAGAGGTACGGCGAGATCTTCAAGACGCACATTCTGGGTTGCCCGTGCGTGATGCTGGCGAGCCCGGAGGCGGCGCGGTTCGTGCTGGTGACGCAGGCGCACCTGTTCAAGCCGACGTACCCGCGGAGCAAGGAGCGGATGATCGGCCCGTCGGCGCTCTTCTTCCACCAGGGCGACTACCACCTCCGCCTTCGCAAGCTCGTCCAGGGCCCTCTCGGCCCCGACGCCCTGCGCGCGCTCGTGCCGGACGTCGAGGCCGCCGTCCGCTCCACGCTCGCCTCCTGGGACGGCAACGTCTCCAGCACCTTCCACGCCATGAAGAGGCTCTCGTTCGATGTCGGCATCGTGACCATCTTCGGCGGGCGGCTGGACGAGCGGCGGAAAGCGGAGCTGAGGCAGAACTACGCCATCGTGGAGAAGGGCTACAACTCCTTCCCCAACAGCTTCCCCGGGACGCTGTACTACAAGGCGATCCAGGCGAGGCGGCGGCTGCACGGCGTGCTGAGCGACATCATGCGGGAGCGGCGGGCGCGGGGGGAGCCCGGCAGCGACCTCCTCGGCTGCCTCATGCAGTCGCGGGCGGGCGACGACGGCGCGCTCCTCACCGACGAGCAGGTCGCCGACAACATCATCGGCGTGCTGTTCGCGGCGCAGGACACGACGGCCAGCGTGCTCACCTGGATCGTCAAGTACCTCCACGACCATCCCAAGCTGCTCGAGGCCGTCAGGGCGGAGCAGGCGGCGATCCGCGCCGCCAACGACGGCGGCCGGCTGCCGCTGACGTGGGCGCAGACGCGGAGCATGGCCCTAACCCACAAGGTGATTTTGGAGAGCTTAAGGATGGCCAGCATCATCTCGTTCACGTTCAGGGAGGCCGTGGCTGACGTGGAGTACAAAGGGTTCCTTATCCCCAAGGGATGGAAGGTGATGCCGCTCTTCAGGAACATCCATCACAACCCAGACTACTTCCAGGATCCACAGAAGTTCGACCCTTCTAGATTCAAGGTGTCGCCGAGGCCGAACACCTTCATGCCATTTGGGAACGGCGTGCACGCGTGCCCCGGGAACGAGCTGGCCAAGCTCGAGATGCTCGTCCTCATCCACCACCTGGTCACTGGCTACAGGTGGGAGATTGTTGGTTCCAGCGACGAGGTTGAGTACAGCCCATTCCCTGTGCCCAAGCATGGCCTTCTCGCGAAATTATGGAGGGATGATAGTGTCAGTGTGGAAACAGATGGTTGCCAGAACGGTGATAATGACGACAATGGCGTAGCAATGGTTTGATTAGGCGTTTAATGTAGATAAGCTAGCTAGGGAGATATTTTTCTTTCTTTCATTGCCCTTCTATTTCCTCAAGGGAAGTCCGGGGAGAAAAAATGGAGTTTTCTTCTTTTTTAACTTGATCATAAGAAGGTAATGATGATGAGAATGGGTTCTCGGAGGAAAAACAAATGATAATGTGTAATATCATTGACATTGACCCAAATTAAATGGCTTCAGCAGTTAATGCTGCTGCATTTCGGCTCCCTTTCA
CDS Sequence
- >LOC_Os09g28390.1
ATGGCAGCCTCCTTCGTCATCGTCATCGTCATCTCCTTCTTCATTTCTCTTGCTTTTATGTGCTATGTCCACTACACGAGCCGGCAGAGGAGGAAACTCCATGGCTACGGCCATGAGAAAGCCGTCAGGCTGCCGCCGGGCTCCATGGGTTGGCCTTACATCGGCGAGACCCTTCAGCTCTACTCCCAAGACCCCAACGTCTTCTTCGCCTCCAAACAGAAGAGGTACGGCGAGATCTTCAAGACGCACATTCTGGGTTGCCCGTGCGTGATGCTGGCGAGCCCGGAGGCGGCGCGGTTCGTGCTGGTGACGCAGGCGCACCTGTTCAAGCCGACGTACCCGCGGAGCAAGGAGCGGATGATCGGCCCGTCGGCGCTCTTCTTCCACCAGGGCGACTACCACCTCCGCCTTCGCAAGCTCGTCCAGGGCCCTCTCGGCCCCGACGCCCTGCGCGCGCTCGTGCCGGACGTCGAGGCCGCCGTCCGCTCCACGCTCGCCTCCTGGGACGGCAACGTCTCCAGCACCTTCCACGCCATGAAGAGGCTCTCGTTCGATGTCGGCATCGTGACCATCTTCGGCGGGCGGCTGGACGAGCGGCGGAAAGCGGAGCTGAGGCAGAACTACGCCATCGTGGAGAAGGGCTACAACTCCTTCCCCAACAGCTTCCCCGGGACGCTGTACTACAAGGCGATCCAGGCGAGGCGGCGGCTGCACGGCGTGCTGAGCGACATCATGCGGGAGCGGCGGGCGCGGGGGGAGCCCGGCAGCGACCTCCTCGGCTGCCTCATGCAGTCGCGGGCGGGCGACGACGGCGCGCTCCTCACCGACGAGCAGGTCGCCGACAACATCATCGGCGTGCTGTTCGCGGCGCAGGACACGACGGCCAGCGTGCTCACCTGGATCGTCAAGTACCTCCACGACCATCCCAAGCTGCTCGAGGCCGTCAGGGCGGAGCAGGCGGCGATCCGCGCCGCCAACGACGGCGGCCGGCTGCCGCTGACGTGGGCGCAGACGCGGAGCATGGCCCTAACCCACAAGGTGATTTTGGAGAGCTTAAGGATGGCCAGCATCATCTCGTTCACGTTCAGGGAGGCCGTGGCTGACGTGGAGTACAAAGGGTTCCTTATCCCCAAGGGATGGAAGGTGATGCCGCTCTTCAGGAACATCCATCACAACCCAGACTACTTCCAGGATCCACAGAAGTTCGACCCTTCTAGATTCAAGGTGTCGCCGAGGCCGAACACCTTCATGCCATTTGGGAACGGCGTGCACGCGTGCCCCGGGAACGAGCTGGCCAAGCTCGAGATGCTCGTCCTCATCCACCACCTGGTCACTGGCTACAGGTGGGAGATTGTTGGTTCCAGCGACGAGGTTGAGTACAGCCCATTCCCTGTGCCCAAGCATGGCCTTCTCGCGAAATTATGGAGGGATGATAGTGTCAGTGTGGAAACAGATGGTTGCCAGAACGGTGATAATGACGACAATGGCGTAGCAATGGTTTGA
Protein Sequence
- >LOC_Os09g28390.1
MAASFVIVIVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQDPNVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASWDGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLWRDDSVSVETDGCQNGDNDDNGVAMV*