Information report for OsrbohB;Osrboh7
Gene Details
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Functional Descriptions
- Moreover, a number of genes involved in plant development, stress response, transcriptional regulation, and particularly ABA signaling are differentially expressed in OsrbohB plants under both normal growth and drought conditions.
- The OsrbohB mutant also exhibits lower seed germination rate, organ size and thousand seed weight, but higher stomatal aperture and sensitivity to drought.
- We found that a rice NADPH oxidase gene OsrbohB contributes drought tolerance and its functions are involved in the interaction of the OsrbohB-mediated ROS production and ABA signaling.
- All these results suggest the roles of OsrbohB in drought tolerance of rice, which probably performed through the interaction of the OsrbohB-mediated ROS production and ABA signaling.
- The transcriptional expression of OsrbohB is also strongly stimulated by dehydration, salt and several phytohormonal treatments.
- Overproduction of OsRACK1A restores the OsRACK1A-OsrbohB association and promotes OsrbohB phosphorylation to enhance ROS production, conferring rice floral resistance to U.
- Further analyses revealed that OsDMI3-mediated OsrbohB Ser-191 phosphorylation positively regulates the activity of NADPH oxidase and the production of H(2)O(2) in ABA signaling, thereby enhancing the sensitivity of seed germination and root growth to ABA and plant tolerance to water stress and oxidative stress.
- Phosphorylation of OsrbohB by the protein kinase OsDMI3 promotes H(2)O(2) production to potentiate ABA responses in rice.
- OsDMI3 directly interacts with and phosphorylates OsrbohB at Ser-191, which is OsDMI3-mediated site-specific phosphorylation in ABA signaling.
- Moreover, we discovered that the OsDMI3-mediated OsrbohB phosphorylation and H(2)O(2) production is dependent on the sucrose non-fermenting 1-related protein kinases SAPK8/9/10, which phosphorylate OsrbohB at Ser-140 in ABA signaling.
Functional Keywords
- growth , plant-development , seed , drought , salt , seed-germination , tolerance , ABA , drought-tolerance , stress , stomatal , seed-weight , organ-size , stress-response , resistance , floral , root , Kinase , kinase , oxidative-stress , oxidative , root-growth , protein-kinase , sucrose , ER-stress
Literature and News
- Reactive oxygen species production and activation mechanism of the rice NADPH oxidase OsRbohB . DOI: 10.1093/jb/mvs044 ; PMID: 22528669
- Phosphatidylinositol 3-kinase plays a vital role in regulation of rice seed vigor via altering NADPH oxidase activity . DOI: 10.1371/journal.pone.0033817 ; PMID: 22448275
- OsRbohB-mediated ROS production plays a crucial role in drought stress tolerance of rice . DOI: 10.1007/s00299-020-02603-2 ; PMID: 32980968
- Overproduction of OsRACK1A, an effector-targeted scaffold protein promoting OsRBOHB-mediated ROS production, confers rice floral resistance to false smut disease without yield penalty . DOI: 10.1016/j.molp.2022.10.009 ; PMID: 36245122
- Rice iron storage protein ferritin 2 (OsFER2) positively regulates ferroptotic cell death and defense responses against Magnaporthe oryzae . DOI: 10.3389/fpls.2022.1019669 ; PMID: 36352872
- Phosphorylation of OsRbohB by the protein kinase OsDMI3 promotes H2O2 production to potentiate ABA responses in rice . DOI: 10.1016/j.molp.2023.04.003 ; PMID: 37029489
Gene Resources
- UniProt: Q69QE0
- EMBL: AP005424, AP005833, AP008215
- EnsemblPlants: Os09t0438000-01
- Gramene: Os09t0438000-01
- KEGG: dosa:Os09g0438000, osa:4347158
- Orthologous matrix: IGRAKFG
- InterPro: IPR000778, IPR002048, IPR011992
- PANTHER: PTHR11972, PTHR11972:SF44
- SUPFAM: SSF47473, SSF52343, SSF63380
- PROSITE: PS50222, PS51384
- Gene3D: 1.10.238.10, 2.40.30.10, 3.40.50.80
- OrthoDB: Q69QE0
- SWISS-MODEL: Q69QE0
- Conserved Domain Database: cd00051, cd06186
Sequences
cDNA Sequence
- >LOC_Os09g26660.1
GAAGGACGCGCAACGCAACCAACGACCGCGCGTCGCGTTGCTCTCTCTCTCTCTCGCAGTCTCGCTTGCTTTGCCCTTGTAGACAGACACTCCCGTGGCGCGGCGCGGGCGCGGCGTGGCGTGGGCGCGGGCGCGGGCGCCCCGGCGGGCATGTGGACGCCGTCGCGCGGGAGCAACGCCGCGCGGCGGAGTGGCCATAGGCGCATCGCGGACTACCTCGCCGACGACCAGACCACCAACACCGACACCTCCGACAACGAGTCATACACCACCGCCTATGGCGATGAGTTCTTCGCCGCGGCGGCGGCGGCGGCCGGGAGTGGAGGGGGTGGTATGCTGCCGGCATTCCTCGCCGACCAGGGGGACCTCGTCGAGGTCATGCTGGAGCTCGACGAGGAGTCCATGGTCGTGCGCAGCGTCACTCCCACCAGCGCCACTCTCTACGGCGGCGGCGGCGGGCAGATGCCACAGCCGCTGCCGCCGCCGCTGCGCACGCCGGAGGGGGGCGGGGGAGCGCGGAGCCTGAGCCGGTGCTCGTCGACGTCGTCGCGGATACGGAAGAAGTTCGCGTGGCTGCGCTCGCCGTCCCCCTCGCCGTCGCCGCGCCCGCCGACGCCCGCGGAGCTGCAGCGCGAGGCGGCCATGGCGGCGCGCGAGCGCCGCCGCATCCAGGCCCGCCTCAACCGCTCCAGCACCGGCGCCAAGCGCGCCCTCAAGGGCCTCCGCTTCATCAGCCGCACCACCGGCACCGTCCAGGCCGCCGAGCTCTGGCGCCGCGTCGAGGACCGCTTCAACGCCCTCGCCCGCGACGGCCTCCTCTCCCGCGACGACTTCGGCGAATGCATCGGAATGGTGGACTCCAAGGAGTTCGCCGTCGGCATCTTCGACGCGCTGGCGCGGCGGCGGCGACAGAATCTGGAGCGGATCACCAGAGAGGAGCTCTACGACTTCTGGCTCCAGATCTCCGACCAGAGCTTCGACGCGCGCCTCCAGATCTTCTTCGACATGGTGGACACCAACGTGGATGGGAGGATAACGAGGGAGGAAGTACAGGAGCTGATCGTCCTGAGCGCGTCGGCGAACAAGCTGGCGAAGCTGAAGGAGCAAGCGGAGGAGTACGCGTCGCTCATCATGGAGGAGCTCGACCCGGAGAACCTCGGCTACATTGAGCTGTGGCAGCTGGAGGCGCTGCTGCTCCAGCGCGACAGCTACATGAATTACAGCCGGCCGCTGAGCACGGCGAGCGGCGCCCAGTGGAGCCAGAACCTCGGCGGCGCCGCCGTGGCGGCGGGGGCGGCAGCAGCAACAGGCGGAGGAGCGCACGCGGCGGTGGCGGCGAGAGGGGGGCAGCAGCAGCAGCAGCAGCAGGAGGGGAGGAGGGGAGGGTGGGGGGTGAGGAAGGCGGCGGCGAGGGTGAGGGTGGCGGCGGAGGAGAACTGGCGGCGGGCGTGGGTGGTGGCGCTGTGGTTCGCCGCCATGGCGTCGCTGTTCGTGTGGAAGTTCGTGCAGTACCGGCGCACGCCGGCGTTCCGGGTGATGGGGTACTGCCTCCCGACGGCGAAGGGCGCCGCCGAGACGCTCAAGCTCAACATGGCGCTCGTCCTCCTCCCCGTCTGCCGCAACACGCTGACGTGGCTCCGCTCCTCCTGGGCCCGCTTCTTCGTCCCCTTCGACGACAACATCACCTTCCACAAGATGATTGCGACGGCGATCGTGGTGGGGATCACGCTGCACGCGGGGAACCATCTGGCGTGCGATTTCCCGAGGCTGATCGCGTCGGGGCCGGAGGAGTACCGGCTGGTGGCGGACGCGTTCGGGCCGGAGAAGCCGACCTACGTGGGGCTCCTCTCGGGCGTGGAGGGCATCACGGGGGTCGCCATGGTGGTGCTCATGACCGTCTCCTTCACGCTGGCCACCCACCCGTTCCGCAAGGGCGAGAAGGGTGGCAGCGGCGGTGGCGCAGCCGCCACCGTCCTCCCCACGGTAGCGCGGCTCCCCTCGCCGTTCAACCGCCTCGCGGGGTTCAACGCCTTCTGGTACTCCCACCACCTCCTCGGCATCGTCTACGCTCTTCTTATCGCCCATGGGTACTTCCTCTTCCTCGTCCGTCGCTGGTATTTGAAAACGACATGGATGTACATTTCTGTTCCATTGATGCTGTATGTCGGTGAAAGGATGCTACGAGCCTTGCGGTCAAATGCTTATGCTGTCAAAATCTTGAAGGTCTGCCTTCTACCTGGAAATGTATTGACCATAACAATGTCAAAGCCCTACGGATTTCGATATAGAAGTGGACAGTACATATTTCTTCAGTGTCCCACAATTTCTCCCTTCGAATGGCATCCCTTCTCCATCACTTCAGCTCCTGGAGATGACTACCTCAGTGTTCACATCCGAACAAACGGAGACTGGACACAGGAGCTGAAGCGCATATTCGTCGAGAACTACTTCTCGCCACACCTTAACAGAAGAGCTTCATTCAGCGAGCTAGGCGCCACAGAACCAAGAAGCTTACCAAGATTGCTCGTCGACGGTCCCTATGGCGCCCCTGCACAGGATTTCAGAAACTACGACGTGCTGCTTCTTGTCGGCCTCGGGATCGGGGCGACACCATTCATCAGCATCCTGAGGGACCTACTTAACAATATTAAGCTAGCTGAAGAGCTGATGGACTTGGCAATGGAGACCAGTAGATCTGAGGACAGTGCCAACAGCTTCAGTGTGTCAACAGCAAGTAGCAACAAGAAGAGGGCATACAGAACAAGCCGCGCACATTTTTACTGGGTTACCCGGGAGCCATTGTCATTTGAGTGGTTCAAAGGAGTGATGAATGAGGTTGCTGAAATGGACAAGAAGGGTGTGATAGAGCTGCACAATTATCTGACGAGCGTTTACGAGGAGCGCGATGCGCGGACAACTCTGCTGTCCATGGTGCAAGCTCTGAACCATGCCAAGCATGGTGTTGACATCGTCTCAGGCACAAGGGTGAGGACACATTTTGCAAGACCAAACTGGAAAGAAGTCTTCACCAGGATTGCCTCCAAGCACCCCAATTCAACAGTTGGAGTTTTCTACTGCGGCGCGCCGACGCTAGCCAAGGAGCTGAAGACCCTGTCCCACGAGATGAGCCACAGGACGGGGACTCGCTTCCATTTCCACAAGGAGTACTTCTGAATTTCTCATAGAAGTAAAAAGAAAAAGGATATAGCTTCTCCTGATCTCCATAGCATTAGGTACGGTAACTAAAGAAGACGAAGACGAAGAAGAAAATTTTTTGCACAGAAGCAAAGCAAAGCTGACCTCTCTTCCTGATCTGGAACTGTACATATAGTATATATAAAGGAGCACACAGTATACAGGGTAGGTAATAGCGATTTATATATAATTAACAAGCAGGGGAAAATGCAGGGTAAAAAAGCGTTGCTAATTTTCGCTATAGACATCATCATACAGATATATACACTACACAGTATAGTTTAGTTTACAATTATAGTTTACAGTACCATGCACCGTTCAAAAAAACATCCTCTCTATCAGCTAGTATTCAGCAGCTAATAAGCTCCAGTTGGCAATAAAATTCGGCAGCAACCATTACCAGTGA
CDS Sequence
- >LOC_Os09g26660.1
ATGTGGACGCCGTCGCGCGGGAGCAACGCCGCGCGGCGGAGTGGCCATAGGCGCATCGCGGACTACCTCGCCGACGACCAGACCACCAACACCGACACCTCCGACAACGAGTCATACACCACCGCCTATGGCGATGAGTTCTTCGCCGCGGCGGCGGCGGCGGCCGGGAGTGGAGGGGGTGGTATGCTGCCGGCATTCCTCGCCGACCAGGGGGACCTCGTCGAGGTCATGCTGGAGCTCGACGAGGAGTCCATGGTCGTGCGCAGCGTCACTCCCACCAGCGCCACTCTCTACGGCGGCGGCGGCGGGCAGATGCCACAGCCGCTGCCGCCGCCGCTGCGCACGCCGGAGGGGGGCGGGGGAGCGCGGAGCCTGAGCCGGTGCTCGTCGACGTCGTCGCGGATACGGAAGAAGTTCGCGTGGCTGCGCTCGCCGTCCCCCTCGCCGTCGCCGCGCCCGCCGACGCCCGCGGAGCTGCAGCGCGAGGCGGCCATGGCGGCGCGCGAGCGCCGCCGCATCCAGGCCCGCCTCAACCGCTCCAGCACCGGCGCCAAGCGCGCCCTCAAGGGCCTCCGCTTCATCAGCCGCACCACCGGCACCGTCCAGGCCGCCGAGCTCTGGCGCCGCGTCGAGGACCGCTTCAACGCCCTCGCCCGCGACGGCCTCCTCTCCCGCGACGACTTCGGCGAATGCATCGGAATGGTGGACTCCAAGGAGTTCGCCGTCGGCATCTTCGACGCGCTGGCGCGGCGGCGGCGACAGAATCTGGAGCGGATCACCAGAGAGGAGCTCTACGACTTCTGGCTCCAGATCTCCGACCAGAGCTTCGACGCGCGCCTCCAGATCTTCTTCGACATGGTGGACACCAACGTGGATGGGAGGATAACGAGGGAGGAAGTACAGGAGCTGATCGTCCTGAGCGCGTCGGCGAACAAGCTGGCGAAGCTGAAGGAGCAAGCGGAGGAGTACGCGTCGCTCATCATGGAGGAGCTCGACCCGGAGAACCTCGGCTACATTGAGCTGTGGCAGCTGGAGGCGCTGCTGCTCCAGCGCGACAGCTACATGAATTACAGCCGGCCGCTGAGCACGGCGAGCGGCGCCCAGTGGAGCCAGAACCTCGGCGGCGCCGCCGTGGCGGCGGGGGCGGCAGCAGCAACAGGCGGAGGAGCGCACGCGGCGGTGGCGGCGAGAGGGGGGCAGCAGCAGCAGCAGCAGCAGGAGGGGAGGAGGGGAGGGTGGGGGGTGAGGAAGGCGGCGGCGAGGGTGAGGGTGGCGGCGGAGGAGAACTGGCGGCGGGCGTGGGTGGTGGCGCTGTGGTTCGCCGCCATGGCGTCGCTGTTCGTGTGGAAGTTCGTGCAGTACCGGCGCACGCCGGCGTTCCGGGTGATGGGGTACTGCCTCCCGACGGCGAAGGGCGCCGCCGAGACGCTCAAGCTCAACATGGCGCTCGTCCTCCTCCCCGTCTGCCGCAACACGCTGACGTGGCTCCGCTCCTCCTGGGCCCGCTTCTTCGTCCCCTTCGACGACAACATCACCTTCCACAAGATGATTGCGACGGCGATCGTGGTGGGGATCACGCTGCACGCGGGGAACCATCTGGCGTGCGATTTCCCGAGGCTGATCGCGTCGGGGCCGGAGGAGTACCGGCTGGTGGCGGACGCGTTCGGGCCGGAGAAGCCGACCTACGTGGGGCTCCTCTCGGGCGTGGAGGGCATCACGGGGGTCGCCATGGTGGTGCTCATGACCGTCTCCTTCACGCTGGCCACCCACCCGTTCCGCAAGGGCGAGAAGGGTGGCAGCGGCGGTGGCGCAGCCGCCACCGTCCTCCCCACGGTAGCGCGGCTCCCCTCGCCGTTCAACCGCCTCGCGGGGTTCAACGCCTTCTGGTACTCCCACCACCTCCTCGGCATCGTCTACGCTCTTCTTATCGCCCATGGGTACTTCCTCTTCCTCGTCCGTCGCTGGTATTTGAAAACGACATGGATGTACATTTCTGTTCCATTGATGCTGTATGTCGGTGAAAGGATGCTACGAGCCTTGCGGTCAAATGCTTATGCTGTCAAAATCTTGAAGGTCTGCCTTCTACCTGGAAATGTATTGACCATAACAATGTCAAAGCCCTACGGATTTCGATATAGAAGTGGACAGTACATATTTCTTCAGTGTCCCACAATTTCTCCCTTCGAATGGCATCCCTTCTCCATCACTTCAGCTCCTGGAGATGACTACCTCAGTGTTCACATCCGAACAAACGGAGACTGGACACAGGAGCTGAAGCGCATATTCGTCGAGAACTACTTCTCGCCACACCTTAACAGAAGAGCTTCATTCAGCGAGCTAGGCGCCACAGAACCAAGAAGCTTACCAAGATTGCTCGTCGACGGTCCCTATGGCGCCCCTGCACAGGATTTCAGAAACTACGACGTGCTGCTTCTTGTCGGCCTCGGGATCGGGGCGACACCATTCATCAGCATCCTGAGGGACCTACTTAACAATATTAAGCTAGCTGAAGAGCTGATGGACTTGGCAATGGAGACCAGTAGATCTGAGGACAGTGCCAACAGCTTCAGTGTGTCAACAGCAAGTAGCAACAAGAAGAGGGCATACAGAACAAGCCGCGCACATTTTTACTGGGTTACCCGGGAGCCATTGTCATTTGAGTGGTTCAAAGGAGTGATGAATGAGGTTGCTGAAATGGACAAGAAGGGTGTGATAGAGCTGCACAATTATCTGACGAGCGTTTACGAGGAGCGCGATGCGCGGACAACTCTGCTGTCCATGGTGCAAGCTCTGAACCATGCCAAGCATGGTGTTGACATCGTCTCAGGCACAAGGGTGAGGACACATTTTGCAAGACCAAACTGGAAAGAAGTCTTCACCAGGATTGCCTCCAAGCACCCCAATTCAACAGTTGGAGTTTTCTACTGCGGCGCGCCGACGCTAGCCAAGGAGCTGAAGACCCTGTCCCACGAGATGAGCCACAGGACGGGGACTCGCTTCCATTTCCACAAGGAGTACTTCTGA
Protein Sequence
- >LOC_Os09g26660.1
MWTPSRGSNAARRSGHRRIADYLADDQTTNTDTSDNESYTTAYGDEFFAAAAAAAGSGGGGMLPAFLADQGDLVEVMLELDEESMVVRSVTPTSATLYGGGGGQMPQPLPPPLRTPEGGGGARSLSRCSSTSSRIRKKFAWLRSPSPSPSPRPPTPAELQREAAMAARERRRIQARLNRSSTGAKRALKGLRFISRTTGTVQAAELWRRVEDRFNALARDGLLSRDDFGECIGMVDSKEFAVGIFDALARRRRQNLERITREELYDFWLQISDQSFDARLQIFFDMVDTNVDGRITREEVQELIVLSASANKLAKLKEQAEEYASLIMEELDPENLGYIELWQLEALLLQRDSYMNYSRPLSTASGAQWSQNLGGAAVAAGAAAATGGGAHAAVAARGGQQQQQQQEGRRGGWGVRKAAARVRVAAEENWRRAWVVALWFAAMASLFVWKFVQYRRTPAFRVMGYCLPTAKGAAETLKLNMALVLLPVCRNTLTWLRSSWARFFVPFDDNITFHKMIATAIVVGITLHAGNHLACDFPRLIASGPEEYRLVADAFGPEKPTYVGLLSGVEGITGVAMVVLMTVSFTLATHPFRKGEKGGSGGGAAATVLPTVARLPSPFNRLAGFNAFWYSHHLLGIVYALLIAHGYFLFLVRRWYLKTTWMYISVPLMLYVGERMLRALRSNAYAVKILKVCLLPGNVLTITMSKPYGFRYRSGQYIFLQCPTISPFEWHPFSITSAPGDDYLSVHIRTNGDWTQELKRIFVENYFSPHLNRRASFSELGATEPRSLPRLLVDGPYGAPAQDFRNYDVLLLVGLGIGATPFISILRDLLNNIKLAEELMDLAMETSRSEDSANSFSVSTASSNKKRAYRTSRAHFYWVTREPLSFEWFKGVMNEVAEMDKKGVIELHNYLTSVYEERDARTTLLSMVQALNHAKHGVDIVSGTRVRTHFARPNWKEVFTRIASKHPNSTVGVFYCGAPTLAKELKTLSHEMSHRTGTRFHFHKEYF*